RESUMO
Silver nanoparticles (AgNPs) are synthesized using biological sources due to its high specificity in biomedical applications. Herein, we report the size and shape controlled synthesis of AgNPs using the aqueous extract of blue green alga, Spirulina platensis. Size, shape and elemental composition of AgNPs were characterized using UV-vis spectroscopy, Fluorescence spectroscopy, FT-IR (Fourier Transform-Infrared Spectroscopy), FT-RS (Fourier Transform-Raman Spectroscopy), SEM-EDAX (Scanning Electron Microscopy-Energy Dispersive X-ray analysis) and HR-TEM (High Resolution Transmission Electron Microscopy). AgNPs were stable, well defined and monodispersed (spherical) with an average size of 6 nm. The synthesized AgNPs were tested for its antibacterial potency against isolates obtained from HIV patients.
Assuntos
Antibacterianos/uso terapêutico , Infecções por HIV/tratamento farmacológico , Infecções por HIV/microbiologia , Nanopartículas Metálicas/química , Tamanho da Partícula , Prata/farmacologia , Spirulina/química , Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Escherichia coli/crescimento & desenvolvimento , Humanos , Nanopartículas Metálicas/ultraestrutura , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/crescimento & desenvolvimento , RNA Ribossômico 16S/genética , Análise de Sequência de RNA , Espectrofotometria Ultravioleta , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman , Difração de Raios XRESUMO
Biofunctionalized gold nanoparticles (AuNPs) play an important role in design and development of nanomedicine. Synthesis of AuNPs from biogenic materials is environmentally benign and possesses high bacterial inhibition and bactericidal properties. In the present study, blue green alga Spirulina platensis protein mediated synthesis of AuNPs and its antibacterial activity against Gram positive bacteria is discussed. AuNPs were characterized using Ultraviolet-visible (UV-vis) spectroscopy, Fluorescence spectroscopy, Fourier Transform-Infrared (FTIR) spectroscopy, Raman spectroscopy, High Resolution-Transmission Electron Microscopy (HR-TEM) and Energy Dispersive X-ray analysis (EDAX). Stable, well defined AuNPs of smaller and uniform shape with an average size of ~ 5 nm were obtained. The antibacterial efficacy of protein functionalized AuNPs were tested against Gram positive organisms Bacillus subtilis and Staphylococcus aureus.
Assuntos
Antibacterianos/química , Antibacterianos/farmacologia , Cianobactérias/química , Ouro/química , Ouro/farmacologia , Bactérias Gram-Positivas/efeitos dos fármacos , Nanopartículas Metálicas/química , Microscopia Eletrônica de Transmissão/métodos , Espectrofotometria Ultravioleta/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Análise Espectral Raman/métodosRESUMO
Pongamia pinnata, Linn., an Indian medicinal plant used in the Ayurvedha and Siddha traditional medicine systems, for treatment of clinical lesions of skin and genitalia, was evaluated for antiviral properties against herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) by in-vitro studies in Vero cells. A crude aqueous seed extract of P. pinnata completely inhibited the growth of HSV-1 and HSV-2 at concentrations of 1 and 20 mg/ml (w/v), respectively, as shown by complete absence of cytopathic effect.
Assuntos
Antivirais/farmacologia , Plantas Medicinais , Sementes , Simplexvirus/efeitos dos fármacos , Animais , Efeito Citopatogênico Viral/efeitos dos fármacos , Relação Dose-Resposta a Droga , Índia , Medicina Tradicional do Leste Asiático , Extratos Vegetais/farmacologia , Células VeroRESUMO
The mechanisms of pathogenicity in EPEC strains were studied in tissue culture. Escherichia coli was isolated as the predominant organism in the primary culture of 1293 (70.54%) diarrhoeal cases. 284 (90.44%) cases from the age group of 1-6 months showed Escherichia coli as the predominant organism. Classical Enteropathogenic Escherichia coli (EPEC) were detected in 311 (24.05%) cases. Among EPEC isolates 277 (89.06%) did not produce either LT or ST. 32 (10.28%) produced LT or ST. 2 strains produced verotoxins belong to serotypes 0:86; K:61, 0.26; K:60, serogroups 0.86:K61 0142:K86, 0:128:K67, 0 126: 71, 0125:K 70, 0119: k69 showed localised adherence and serogroups 0111:K58-055:K59 showed both localised and diffused adherence to HeLa cells.
Assuntos
Diarreia/microbiologia , Escherichia coli/patogenicidade , Aderência Bacteriana , Pré-Escolar , Enterotoxinas/biossíntese , Escherichia coli/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Humanos , Lactente , Recém-NascidoRESUMO
The mechanisms of pathogenicity in EPEC strains were studied in tissue culture. Escherichia coli was isolated as the predominant organism in the primary culture of 1293 (70.54%) diarrhoeal cases. 284 (90.44%) cases from the age group of 1-6 months showed Escherichia coli as the predominant organism. Classical Enteropathogenic Escherichia coli) were detected in 311 (24.05%) cases. Among EPEC isolates 277 (89.06%) did not produce either LT or ST 32(10.28%) produced LT or ST. 2 strains produced verotoxins belong to serotypes 0:86; K:61, 0:26; K:60, sero groups 0.86 :K:61, 0.142:K 86, 0.128:K 67, 0.126:K 71, 0125:K 70 0119:K69 showed localised adherence and serogroups 0111:K58-055:K59 showed both localised and diffused adherence to HeLa cells.
