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1.
J Phys Chem B ; 128(6): 1438-1447, 2024 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-38316620

RESUMO

The Milestoning algorithm is a method for long-time molecular dynamics simulations. It enables the sampling of rare events. The precise calculations of observables depend on accurately determining the first hitting point distribution (FHPD) for each milestone. There is no analytical expression for FHPD, which is estimated numerically. Several variants of Milestoning offer approximations to the FHPD. Here, we examine in detail the FHPD of an exact calculation and Milestoning variants. We also introduce a new version of the Milestoning algorithm, buffer Milestoning, with a comparable cost to conventional Milestoning but higher accuracy. We use the mean first passage time and the free energy to assess the simulation quality, and we compare the accuracy and efficiency of buffer Milestoning to exact calculations, conventional Milestoning, local-passage-time-weighted Milestoning, Markovian Milestoning with Voronoi tessellation, and exact Milestoning. Conventional Milestoning requires milestone decorrelation. If this condition is not satisfied, it is the least accurate approach of all the techniques we examined. We conclude that for a small increase in cost compared to conventional Milestoning, buffer Milestoning provides accurate results for a range of problems, including more correlated milestones and is, therefore, versatile compared to other variants. Local-passage-time-weighted Milestoning provides accuracy similar to that of buffer Milestoning but at an increased simulation cost. Markovian Milestoning with Voronoi tessellation is the most accurate compared with other approximations, but it is less stable for high barriers and more expensive.

2.
J Phys Chem B ; 127(41): 8796-8808, 2023 10 19.
Artigo em Inglês | MEDLINE | ID: mdl-37815452

RESUMO

Biological processes require DNA and RNA helices to pack together in specific interhelical orientations. While electrostatic repulsion between backbone charges is expected to be maximized when helices are in parallel alignment, such orientations are commonplace in nature. To better understand how the repulsion is overcome, we used experimental and computational approaches to investigate how the orientational preferences of DNA helices depend on the concentration and valence of mobile cations. We used Förster resonance energy transfer (FRET) to probe the relative orientations of two 24-bp helices held together via a freely rotating PEG linker. At low cation concentrations, the helices preferred more "cross"-like orientations over those closer to parallel, and this preference was reduced with increasing salt concentrations. The results were in good quantitative agreement with Poisson-Boltzmann (PB) calculations for monovalent salt (Na+). However, PB underestimated the ability of mixtures of monovalent and divalent ions (Mg2+) to reduce the conformational preference. As a complementary approach, we performed all-atom molecular dynamics (MD) simulations and found better agreement with the experimental results. While MD and PB predict similar electrostatic forces, MD predicts a greater accumulation of Mg2+ in the ion atmosphere surrounding the DNA. Mg2+ occupancy is predicted to be greater in conformations close to the parallel orientation than in conformations close to the crossed orientation, enabling a greater release of Na+ ions and providing an entropic gain (one bound ion for two released). MD predicts an entropy gain larger than that of PB because of the increased Mg2+ occupancy. The entropy changes have a negligible effect at low Mg2+ concentrations because the free energies are dominated by electrostatic repulsion. However, as the Mg2+ concentration increases, charge screening is more effective and the mixing entropy produces readily detectable changes in packing preferences. Our results underline the importance of mixing entropy of counterions in nucleic acid interactions and provide a new understanding on the impact of a mixed ion atmosphere on the packing of DNA helices.


Assuntos
DNA , Transferência Ressonante de Energia de Fluorescência , Entropia , Conformação de Ácido Nucleico , DNA/metabolismo , Simulação de Dinâmica Molecular , Cátions , Sódio , Cloreto de Sódio , Eletricidade Estática
3.
J Phys Chem B ; 127(37): 7810-7818, 2023 09 21.
Artigo em Inglês | MEDLINE | ID: mdl-37678235

RESUMO

Cell penetrating peptides (CPPs) are natural agents that efficiently permeate biological membranes. They are frequently positively charged, which is surprising since membranes pose hydrophobic barriers. In this Perspective, I discuss computations and experiments of a permeation model that couples permeant displacement with a membrane defect. We call the proposed mechanism Defect Assisted by Charge (DAC) and illustrate that it reduces the free energy barrier for translocation. A metastable state at the center of the membrane may be observed due to the charge interactions with the phospholipid head groups at the two leaflets. The combination of experiments and simulations sheds light on the mechanisms of a charged peptide translocation across phospholipid membranes.


Assuntos
Peptídeos Penetradores de Células , Membranas , Membrana Celular , Fosfolipídeos
4.
J Chem Theory Comput ; 19(19): 6816-6826, 2023 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-37695680

RESUMO

Milestoning is an efficient method for rare event kinetics calculation using short trajectory parallelization. Mean first passage time (MFPT) is the key kinetic output of Milestoning, whose accuracy crucially depends on the initial distribution of the short trajectory ensemble. The true initial distribution, i.e., the first hitting point distribution (FHPD), has no analytic expression in the general case. Here, we introduce two algorithms, local passage time weighted Milestoning (LPT-M) and Bayesian inference Milestoning (BI-M), to accurately and efficiently approximate FHPD for systems at equilibrium condition. Starting from sampling the Boltzmann distribution on milestones, we calculate the proper weighting factor for the short trajectory ensemble. The methods are tested on two model examples for illustration purpose. Both methods improve significantly over the widely used classical Milestoning (CM) method in terms of the accuracy of MFPT. In particular, BI-M covers the directional Milestoning method as a special case in deterministic Hamiltonian dynamics. LPT-M is especially advantageous in terms of computational costs and robustness with respect to the increasing number of intermediate milestones. Furthermore, a locally iterative correction algorithm for nonequilibrium stationary FHPD is developed for exact MFPT calculation, which can be combined with LPT-M/BI-M and is much cheaper than the exact Milestoning (ExM) method.

5.
Cell Death Discov ; 9(1): 325, 2023 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-37652915

RESUMO

Cell Penetrating Peptides (CPPs) are promising anticancer and antimicrobial drugs. We recently reported that a peptide derived from the human mitochondrial/ER membrane-anchored NEET protein, Nutrient Autophagy Factor 1 (NAF-1; NAF-144-67), selectively permeates and kills human metastatic epithelial breast cancer cells (MDA-MB-231), but not control epithelial cells. As cancer cells alter their phenotype during growth and metastasis, we tested whether NAF-144-67 would also be efficient in killing other human epithelial breast cancer cells that may have a different phenotype. Here we report that NAF-144-67 is efficient in killing BT-549, Hs 578T, MDA-MB-436, and MDA-MB-453 breast cancer cells, but that MDA-MB-157 cells are resistant to it. Upon closer examination, we found that MDA-MB-157 cells display a high content of intracellular vesicles and cellular protrusions, compared to MDA-MB-231 cells, that could protect them from NAF-144-67. Inhibiting the formation of intracellular vesicles and dynamics of cellular protrusions of MDA-MB-157 cells, using a protein translation inhibitor (the antibiotic Cycloheximide), rendered these cells highly susceptible to NAF-144-67, suggesting that under certain conditions, the killing effect of CPPs could be augmented when they are applied in combination with an antibiotic or chemotherapy agent. These findings could prove important for the treatment of metastatic cancers with CPPs and/or treatment combinations that include CPPs.

6.
J Phys Chem Lett ; 14(28): 6349-6354, 2023 Jul 20.
Artigo em Inglês | MEDLINE | ID: mdl-37418426

RESUMO

Passive permeation of small molecules into vesicles with multiple compartments is a critical event in many chemical and biological processes. We consider the translocation of the peptide NAF-144-67 labeled with a fluorescent fluorescein dye across membranes of rhodamine-labeled 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) into liposomes with internal vesicles. Time-resolved microscopy revealed a sequential absorbance of the peptide in both the outer and inner micrometer vesicles that developed over a time period of minutes to hours, illustrating the spatial and temporal progress of the permeation. There is minimal perturbation of the membrane structure and no evidence for pore formation. On the basis of molecular dynamics simulations of NAF-144-67, we extended a local defect model to migration processes that include multiple compartments. The model captures the long residence time of the peptide within the membrane and the rate of permeation through the liposome and its internal compartments. Imaging experiments confirm the semi-quantitative description of the permeation of the model by activated diffusion and open the way for studies of more complex systems.


Assuntos
Lipossomos , Fosfolipídeos , Fosfolipídeos/química , Lipossomos/química , Fenômenos Químicos , Corantes Fluorescentes/química , Peptídeos , Bicamadas Lipídicas/química , Fosfatidilcolinas/química
7.
Proteins ; 91(2): 209-217, 2023 02.
Artigo em Inglês | MEDLINE | ID: mdl-36104870

RESUMO

As drug-binding kinetics has become an important factor to be considered in modern drug discovery, this work evaluated the ability of the Milestoning method in computing the absolute dissociation rate of a ligand from the serine-threonine kinase, glycogen synthase kinase 3ß, which is a target for designing drugs to treat diseases such as neurodegenerative disorders and diabetes. We found that the Milestoning method gave good agreement with experiment with modest computational costs. Although the time scale for dissociation lasted tens of seconds, the collective molecular dynamics simulations total less than 1µs. Computing the committor function helped to identify the transition states (TSs), in which the ligand moved substantially away from the binding pocket. The glycine-rich loop with a serine residue attaching to its tips was found to undergo large movement from the bound to the TSs and might play a role in controlling drug-dissociation kinetics.


Assuntos
Simulação de Dinâmica Molecular , Ligantes , Quinases da Glicogênio Sintase , Glicogênio Sintase Quinase 3 beta
8.
J Chem Theory Comput ; 18(11): 6952-6965, 2022 Nov 08.
Artigo em Inglês | MEDLINE | ID: mdl-36191005

RESUMO

Milestoning is a theory and an algorithm that computes kinetics and thermodynamics at long time scales. It is based on partitioning the (phase) space into cells and running a large number of short trajectories between the boundaries of the cells. The termination points of the trajectories are analyzed with the Milestoning theory to obtain kinetic and thermodynamic information. Managing the tens to hundreds of thousands of Milestoning trajectories is a challenge, which we handle with a python script, ScMiles. Here, we introduce a new version of the python script ScMiles2 to conduct Milestoning simulations. Major enhancements are: (i) post analysis of Milestoning trajectories to obtain the free energy, mean first passage time, the committor function, and exit times; (ii) similar to (i) but the post analysis is for a single long trajectory; (iii) we support the use of the GROMACS software in addition to NAMD; (iv) a restart option; (v) the automated finding, sampling, and launching trajectories from new milestones that are found on the fly; and (vi) support Milestoning calculations with several coarse variables and for complex reaction coordinates. We also evaluate the simulation parameters and suggest new algorithmic features to enhance the rate of convergence of observables. We propose the use of an iteration-averaged kinetic matrix for a rapid approach to asymptotic values. Illustrations are provided for small systems and one large example.


Assuntos
Algoritmos , Simulação de Dinâmica Molecular , Cinética , Termodinâmica , Simulação por Computador
9.
Life (Basel) ; 12(10)2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36294908

RESUMO

We conduct molecular dynamics simulations of model heterogeneous membranes and their interactions with a 24-amino acid peptide-NAF-144-67. NAF-144-67 is an anticancer peptide that selectively permeates and kills malignant cells; it does not permeate normal cells. We examine three membranes with different binary mixtures of lipids, DOPC-DOPA, DOPC-DOPS, and DOPC-DOPE, with a single peptide embedded in each as models for the diversity of biological membranes. We illustrate that the peptide organization in the membrane depends on the types of nearby phospholipids and is influenced by the charge and size of the head groups. The present study sheds light on early events of permeation and the mechanisms by which an amphiphilic peptide crosses from an aqueous solution to a hydrophobic membrane. Understanding the translocation mechanism is likely to help the design of new permeants.

10.
J Phys Chem B ; 126(34): 6454-6463, 2022 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-35997537

RESUMO

A physical understanding of membrane permeation and translocation by small, positively charged molecules can illuminate cell penetrating peptide mechanisms of entry and inform drug design. We have previously investigated the permeation of the doubly charged peptide WKW and proposed a defect-assisted permeation mechanism where a small molecule with +2 charge can achieve a metastable state spanning the bilayer by forming a membrane defect with charges stabilized by phospholipid phosphate groups. Here, we investigate the membrane permeation of two doubly charged peptides, WWK and WWWK, with charges separated by different lengths. Through complementary experiments and molecular dynamics simulations, we show that membrane permeation was an order of magnitude more favorable when charges were separated by an ∼2-3 Šgreater distance on WWWK compared to WWK. These results agree with the previously proposed defect-assisted permeation mechanism, where a greater distance between positive charges would require a less extreme membrane defect to stabilize the membrane-spanning metastable state. We discuss the implications of these results in understanding the membrane permeation of cell-penetrating peptides and other small, positively charged membrane permeants.


Assuntos
Peptídeos Penetradores de Células , Bicamadas Lipídicas , Peptídeos Penetradores de Células/química , Bicamadas Lipídicas/química , Simulação de Dinâmica Molecular
11.
Chem Sci ; 13(23): 6929-6941, 2022 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-35774163

RESUMO

An effective anti-cancer therapy should exclusively target cancer cells and trigger in them a broad spectrum of cell death pathways that will prevent avoidance. Here, we present a new approach in cancer therapy that specifically targets the mitochondria and ER of cancer cells. We developed a peptide derived from the flexible and transmembrane domains of the human protein NAF-1/CISD2. This peptide (NAF-144-67) specifically permeates through the plasma membranes of human epithelial breast cancer cells, abolishes their mitochondria and ER, and triggers cell death with characteristics of apoptosis, ferroptosis and necroptosis. In vivo analysis revealed that the peptide significantly decreases tumor growth in mice carrying xenograft human tumors. Computational simulations of cancer vs. normal cell membranes reveal that the specificity of the peptide to cancer cells is due to its selective recognition of their membrane composition. NAF-144-67 represents a promising anti-cancer lead compound that acts via a unique mechanism.

12.
J Phys Chem B ; 126(15): 2834-2849, 2022 04 21.
Artigo em Inglês | MEDLINE | ID: mdl-35388695

RESUMO

Cell-penetrating peptides (CPPs) facilitate translocation across biological membranes and are of significant biological and medical interest. Several CPPs can permeate into specific cells and organelles. We examine the incorporation and translocation of a novel anticancer CPP in a dioleoylphosphatidylcholine (DOPC) lipid bilayer membrane. The peptide, NAF-144-67, is a short fragment of a transmembrane protein, consisting of hydrophobic N-terminal and charged C-terminal segments. Experiments using fluorescently labeled NAF-144-67 in ∼100 nm DOPC vesicles and atomically detailed simulations conducted with Milestoning support a model in which a significant barrier for peptide-membrane entry is found at the interface between the aqueous solution and membrane. The initial step is the insertion of the N-terminal segment and the hydrophobic helix into the membrane, passing the hydrophilic head groups. Both experiments and simulations suggest that the free energy difference in the first step of the permeation mechanism in which the hydrophobic helix crosses the phospholipid head groups is -0.4 kcal mol-1 slightly favoring motion into the membrane. Milestoning calculations of the mean first passage time and the committor function underscore the existence of an early polar barrier followed by a diffusive barrierless motion in the lipid tail region. Permeation events are coupled to membrane fluctuations that are examined in detail. Our study opens the way to investigate in atomistic resolution the molecular mechanism, kinetics, and thermodynamics of CPP permeation to diverse membranes.


Assuntos
Peptídeos Penetradores de Células , Fosforilcolina , Peptídeos Penetradores de Células/química , Cinética , Bicamadas Lipídicas/química , Termodinâmica
13.
J Phys Chem B ; 125(33): 9547-9556, 2021 08 26.
Artigo em Inglês | MEDLINE | ID: mdl-34396776

RESUMO

The binding of phosphatidylinositol 4,5-bisphosphate (PIP2) to the ion channel transient receptor potential vanilloid 5 (TRPV5) is critical for its function. We use atomically detailed simulations and the milestoning theory to compute the free energy profile and the kinetics of PIP2 binding to TRPV5. We estimate the rate of binding and the impact of the protonation state on the process. Several channel residues are identified as influential in the association event and will be interesting targets for mutation analysis. Our simulations reveal that PIP2 binds to TRPV5 in an unprotonated state and is protonated in the membrane. The switch between the protonation state of PIP2 is modeled as a diabatic transition and occurs about halfway through the reaction.


Assuntos
Fosfatidilinositol 4,5-Difosfato , Fosfatidilinositóis , Fenômenos Biofísicos , Cinética , Termodinâmica
14.
J Phys Chem B ; 125(27): 7573-7574, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-34192468
15.
J Phys Chem B ; 125(22): 5706-5715, 2021 06 10.
Artigo em Inglês | MEDLINE | ID: mdl-33930271

RESUMO

Gleevec (a.k.a., imatinib) is an important anticancer (e.g., chronic myeloid leukemia) chemotherapeutic drug due to its inhibitory interaction with the Abl kinase. Here, we use atomically detailed simulations within the Milestoning framework to study the molecular dissociation mechanism of Gleevec from Abl kinase. We compute the dissociation free energy profile, the mean first passage time for unbinding, and explore the transition state ensemble of conformations. The milestones form a multidimensional network with average connectivity of about 2.93, which is significantly higher than the connectivity for a one-dimensional reaction coordinate. The free energy barrier for Gleevec dissociation is estimated to be ∼10 kcal/mol, and the exit time is ∼55 ms. We examined the transition state conformations using both, the committor and transition function. We show that near the transition state the highly conserved salt bridge K217 and E286 is transiently broken. Together with the calculated free energy profile, these calculations can advance the understanding of the molecular interaction mechanisms between Gleevec and Abl kinase and play a role in future drug design and optimization studies.


Assuntos
Leucemia Mielogênica Crônica BCR-ABL Positiva , Simulação por Computador , Humanos , Mesilato de Imatinib , Leucemia Mielogênica Crônica BCR-ABL Positiva/tratamento farmacológico , Conformação Molecular
16.
J Phys Chem B ; 125(14): 3494-3500, 2021 04 15.
Artigo em Inglês | MEDLINE | ID: mdl-33819040

RESUMO

The protein HIV Reverse Transcriptase (HIV RT) synthesizes a DNA strand according to a template. During the synthesis, the polymerase slides on the double stranded DNA to allow the entry of a new nucleotide to the active site. We use Molecular Dynamics simulations to estimate the free energy profile and the time scale of the DNA-protein's relative displacement in the complex's closed state. We illustrate that the presence of the catalytic magnesium slows down the process. Upon removing the catalytic magnesium ion, the process is rapid and significantly faster than reopening the active site in preparation for the new substrate. We speculate that magnesium regulates DNA translocation. The magnesium locks the DNA into a specific orientation during the chemical addition of the nucleotide. The release of Mg2+ eases DNA sliding and the acceptance of a new substrate.


Assuntos
Transcriptase Reversa do HIV , Magnésio , Domínio Catalítico , DNA , DNA Viral , Transcriptase Reversa do HIV/genética , Transcriptase Reversa do HIV/metabolismo , Nucleotídeos
17.
J Phys Chem B ; 125(5): 1343-1350, 2021 02 11.
Artigo em Inglês | MEDLINE | ID: mdl-33507760

RESUMO

Phospholipid membranes support essential biochemical processes, yet remain difficult to characterize due to their compositional and structural heterogeneity. The two most common phospholipid headgroup structures in biological membranes are phosphatidylcholine (PC) and phosphatidylethanolamine (PE), but interactions between PC and PE lipids remain underexplored. In this study, we apply ultrafast two-dimensional infrared (2D IR) spectroscopy to quantify the headgroup effects on interfacial dynamics in PC/PE lipid mixtures. Experiments are interpreted through molecular dynamics simulations using the molecular dynamics with alchemical step (MDAS) algorithm for enhanced sampling. Experimental results indicate that the PE content decreases H-bond formation at the ester carbonyl positions near the lipid membrane's hydrophobic core as a result of increased packing density. The observed dehydration is linked to faster molecular dynamics within the interfacial region.


Assuntos
Bicamadas Lipídicas , Fosfatidilcolinas , Membrana Celular , Membranas , Simulação de Dinâmica Molecular
18.
bioRxiv ; 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33299995

RESUMO

Severe acute respiratory syndrome (SARS) and novel coronavirus disease (COVID-19) are caused by two closely related beta-coronaviruses, SARS-CoV and SARS-CoV-2, respectively. The envelopes surrounding these viruses are decorated with spike proteins, whose receptor binding domains (RBDs) initiate invasion by binding to the human angiotensin-converting enzyme 2 (ACE2). Subtle changes at the interface with ACE2 seem to be responsible for the enhanced affinity for the receptor of the SARS-CoV-2 RBD compared to SARS-CoV RBD. Here, we use Elastic Network Models (ENMs) to study the response of the viral RBDs and ACE2 upon dissassembly of the complexes. We identify a dominant detachment mode, in which the RBD rotates away from the surface of ACE2, while the receptor undergoes a conformational transition which stretches the active-site cleft. Using the Structural Perturbation Method, we determine the network of residues, referred to as the Allostery Wiring Diagram (AWD), which drives the large-scale motion activated by the detachment of the complex. The AWD for SARS-CoV and SARS-CoV-2 are remarkably similar, showing a network that spans the interface of the complex and reaches the active site of ACE2, thus establishing an allosteric connection between RBD binding and receptor catalytic function. Informed in part by the AWD, we used Molecular Dynamics simulations to probe the effect of interfacial mutations in which SARS-CoV-2 residues are replaced by their SARS-CoV counterparts. We focused on a conserved glycine (G502 in SARS-CoV-2, G488 in SARS-CoV) because it belongs to a region that initiates the dissociation of the complex along the dominant detachment mode, and is prominent in the AWD. Molecular Dynamics simulations of SARS-CoV-2 wild-type and G502P mutant show that the affinity for the human receptor of the mutant is drastically diminished. Our results suggest that in addition to residues that are in direct contact with the interface those involved in long range allosteric communication are also a determinant of the stability of the RBD-ACE2 complex.

19.
J Chem Phys ; 153(14): 144110, 2020 Oct 14.
Artigo em Inglês | MEDLINE | ID: mdl-33086798

RESUMO

Computational determination of the equilibrium state of heterogeneous phospholipid membranes is a significant challenge. We wish to explore the rich phase diagram of these multi-component systems. However, the diffusion and mixing times in membranes are long compared to typical time scales of computer simulations. Here, we evaluate the combination of the enhanced sampling techniques molecular dynamics with alchemical steps and Monte Carlo with molecular dynamics with a coarse-grained model of membranes (Martini) to reduce the number of steps and force evaluations that are needed to reach equilibrium. We illustrate a significant gain compared to straightforward molecular dynamics of the Martini model by factors between 3 and 10. The combination is a useful tool to enhance the study of phase separation and the formation of domains in biological membranes.


Assuntos
Membrana Celular/metabolismo , Bicamadas Lipídicas/metabolismo , 1,2-Dipalmitoilfosfatidilcolina/química , Algoritmos , Membrana Celular/química , Bicamadas Lipídicas/química , Simulação de Dinâmica Molecular , Método de Monte Carlo , Fosfatidilserinas/química
20.
J Phys Chem B ; 124(38): 8240-8248, 2020 09 24.
Artigo em Inglês | MEDLINE | ID: mdl-32840372

RESUMO

Extensive experimental studies on the folding of cytochrome c (Cyt c) make this small protein an ideal target for atomic detailed simulations for the purposes of quantitatively characterizing the structural transitions and the associated time scales for folding to the native state from an ensemble of unfolded states. We use previously generated atomically detailed folding trajectories by the stochastic difference equation in length to calculate the time-dependent changes in the small-angle X-ray scattering (SAXS) profiles. Excellent agreement is obtained between experiments and simulations for the time-dependent SAXS spectra, allowing us to identify the structures of the folding intermediates, which shows that Cyt c reaches the native state by a sequential folding mechanism. Using the ensembles of structures along the folding pathways, we show that compaction and the sphericity of Cyt c change dramatically from the prolate ellipsoid shape in the unfolded state to the spherical native state. Our data, which are in unprecedented quantitative agreement with all aspects of time-resolved SAXS experiments, show that hydrophobic collapse and amide group protection coincide on the 100 microseconds time scale, which is in accordance with ultrafast hydrogen/deuterium exchange studies. Based on these results, we propose that compaction of polypeptide chains, accompanied by dramatic shape changes, is a universal characteristic of globular proteins, regardless of the underlying folding mechanism.


Assuntos
Citocromos c , Dobramento de Proteína , Citocromos c/metabolismo , Cinética , Espalhamento a Baixo Ângulo , Difração de Raios X
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