Immuno-histologic and histomorphometric evaluation of Angelica sinensis adjunctive to ß-tricalcium phosphate in critical-sized class II furcation defects in dogs.

OBJECTIVE: The study evaluates the effectiveness of Angelica sinensis (As) adjunctive to Beta-tricalcium phosphate (ß-TCP) bone graft in the management of induced critical sized class II furcation defects in dogs. MATERIAL AND METHOD: A randomized study design was conducted on the third and fourth premolars of six dogs. A total of twenty-four defects were surgically created. After reflecting a mucoperiosteal flap, twelve defects were filled with As granules mixed with ß-TCP (Experimental group) while the other twelve defects were filled with ß-TCP only (Control group) and both were covered by collagen membrane. At the fourth and eighth weeks, jaw segments were dissected and processed for immune-histological examination and histomorphometry analysis. RESULTS: At four and eight weeks after treatment, experimental group showed a statistically significant increase in the height of newly formed interradicular bone (p = 0.001 and p = 0.0001 respectively), its surface area (p = 0.002 and p = 0.02 respectively), and the thickness of its trabeculae (p = 0.0001 and p = 0.001 respectively), when compared to control group. Moreover. alkaline phosphatase immunoreaction showed higher intensity in the osteoblast cells of experimental group compared to control group. CONCLUSION: As enhances periodontal regeneration and bone-formation when used in the management of furcation defects.

Histologic and histomorphometric evaluation of Aloe vera adjunctive to ß-tricalcium phosphate in class II furcation defects in dogs.

Aloe vera has antimicrobial activity and enhances the osseointegration process, thus it may have the potential in treating periodontal defects. This study aimed to evaluate the effect of Aloe vera as an adjunction to Beta-tricalcium phosphate (ß-TCP) bone graft in Grade II furcation defects. A randomized study was conducted on six healthy mongrel dogs' premolars. A total of twenty-four Grade II furcation critical-sized defects were surgically created after reflecting a full-thickness flap, twelve defects were filled with ß-TCP while the other twelve defects were filled with Aloe vera mixed with ß-TCP and both covered by collagen membrane. Animals were euthanized at the end of the fourth and eighth week and defects were analyzed histologically and histomorphometrically. Histologically, Aloe vera mixed with ß-TCP resulted in more bone formation and new PDL fibers compared to ß-TCP alone. After 2 and 4 weeks, the experimental group had significantly higher newly formed interradicular bone height (p < 0.0001, and p < 0.0001, respectively), bone thickness (p < 0.0001, and p < 0.0001, respectively), and percentage of the surface area (p = 0.009, and p = 0.023, respectively). Aloe vera gel adjunctive to ß-TCP is an effective bioactive agent that enhances periodontal tissue regeneration and bone formation in critically sized defects.