RESUMO
The magnetic particles modified with silicon dioxide (SiO2) and amino groups (-NH2), as well as the magnetic particles modified with human serum albumin (HSA) were synthesized using the approaches we developed before and characterized by physico-chemical methods in this study. Plasminogen was chosen as a model protein since plasminogen plays a major role in the fibrinolytic system and plasminogen level correlates with different pathologies and conditions. For the first time it has been carried out qualitative and quantitative assessment of plasminogen nonspecific binding (noncovalent adsorption) by the particles in buffer and plasma solutions. The fibrinolytic activity of plasminogen on the surface of the particles has been measured by the aid of commercially available kits and appeared to be 28-30% of its initial value. Plasminogen desorption from the surface of particles was studied in phosphate buffer with NaCl and ε-aminocaproic acid. Despite nonspecific plasminogen binding is an undesirable process, the data obtained is valuable for further modification of particles for high-specific proteins extraction from biological fluids or transport of plasminogen by the particles. The perspectives of particles modified with SiO2 and -NH2, and particles modified with HSA for isolation of protein analytes and their quantitative assessment thereafter have been discussed.
