Purification, composition and biological activities of a novel heteropolysaccharide extracted from Linum usitatissimum L. seeds on laser burn wound.

The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model. LWSP is a heteropolysaccharide that consists of glucose, mannose, xylose and arabinose. Three different tests were used to evaluate the antioxidant activity of this carbohydrate. The scavenging activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical at a concentration of 5 mg/ml was 99.77%. The total antioxidant capacity of LWSP at 12 mg/ml was equivalent to 166.61 µg acsorbic acid. LWSP displayed a high protection effect against DNA damage induced byhydroxyl radical. No hemolytic activity was observed towards human erythrocytes. LWSP was tested in functional properties. The results showed good emulsion properties and high water (WHC) and oil holding (OHC) capacities (11.23 and 1.05%, respectively). In addition, the application of LWSP on the burn wound site in rat model increased significantly the percentage of burn contraction (98.6%) after 8 days of injury. According to the histological assessment, the LWSP-treated group had a higher content of hydroxyproline (846. 67 ±â€¯92.28 mg/g tissue) than the other groups. Overall, the findings demonstrate that the application of this novel LWSP may open promising opportunities for burn wound healing in modern medicine.

Novel Sorghum bicolor (L.) seed polysaccharide structure, hemolytic and antioxidant activities, and laser burn wound healing effect.

This work was undertaken to determine the structural characteristics of a novel polysaccharide, Sorghum Water-Soluble Polysaccharide (SWSP), extracted from Sorghum (Sorghum bicolor (L.)) seeds and to assess its in vitro hemolytic and antioxidant activities as well as its in vivo wound healing ability to treat burns induced by fractional CO2 laser. Results obtained by 13C, 1H and HSQC Nuclear magnetic resonance (NMR) indicated that SWSP showed the characteristic bands of polysaccharides. Scanning electron microscopy of SWSP showed a rough surface with numerous cavities. SWSP exhibited strong antioxidant activities determined through three different assays: ferrous chelating activity, reducing power and DNA nicking. Also, no hemolytic activity was detected towards human erythrocytes. On the other hand, the application of SWSP hydrogel on the burn site in a rat model significantly improved wound appearance and accelerated the wound closure after 8 days of induction. Histological assessment of biopsies also demonstrated complete re-epithelialized wounds with a full epidermal regeneration. Thus, SWSP hydrogel which was efficient on wound closure might be useful as a wound healing agent in modern medicine.