Posted to Germany: Early Cold War Canadian Military Policy and Its Impact on One Family's Experience.

In the summer of 1954, military surgeon Major Robert Elliott was posted to the British Military Hospital in Iserlohn, Germany, to provide medical care to Canadian soldiers, members of the 5,500-strong Canadian Brigade that had earlier been stationed there as part of Canada's commitment to the North Atlantic Treaty Organization. Like many other military families, Elliott's family had to remain behind until suitable accommodation for them could be found. Based on the letters that Elliott wrote home to his wife during their eight-month separation, this article provides a glimpse of how both old and new Canadian military policies during the early Cold War period had an impact on his work and his family. The Canadian government's decision to place the Brigade under British control reflected, in part, the long-standing attachment to Britain, but Elliott was often frustrated with how imperial/colonial relations played out in the hospital setting. And the military's initial reluctance to officially allow dependents to join their loved ones overseas, a new phenomenon in Canadian military life, undoubtedly contributed to his confusion and anxiety over when family quarters would finally be finished.

Posted to Germany: Early Cold War Canadian Military Policy and Its Impact on One Family's Experience.

In the summer of 1954, military surgeon Major Robert Elliott was posted to the British Military Hospital in Iserlohn, Germany, to provide medical care to Canadian soldiers, members of the 5,500-strong Canadian Brigade that had earlier been stationed there as part of Canada's commitment to the North Atlantic Treaty Organization. Like many other military families, Elliott's family had to remain behind until suitable accommodation for them could be found. Based on the letters that Elliott wrote home to his wife during their eight-month separation, this article provides a glimpse of how both old and new Canadian military policies during the early Cold War period had an impact on his work and his family. The Canadian government's decision to place the Brigade under British control reflected, in part, the long-standing attachment to Britain, but Elliott was often frustrated with how imperial/colonial relations played out in the hospital setting. And the military's initial reluctance to officially allow dependents to join their loved ones overseas, a new phenomenon in Canadian military life, undoubtedly contributed to his confusion and anxiety over when family quarters would finally be finished.

αB-crystallin is a sensor for assembly intermediates and for the subunit topology of desmin intermediate filaments.

Mutations in the small heat shock protein chaperone CRYAB (αB-crystallin/HSPB5) and the intermediate filament protein desmin, phenocopy each other causing cardiomyopathies. Whilst the binding sites for desmin on CRYAB have been determined, desmin epitopes responsible for CRYAB binding and also the parameters that determine CRYAB binding to desmin filaments are unknown. Using a combination of co-sedimentation centrifugation, viscometric assays and electron microscopy of negatively stained filaments to analyse the in vitro assembly of desmin filaments, we show that the binding of CRYAB to desmin is subject to its assembly status, to the subunit organization within filaments formed and to the integrity of the C-terminal tail domain of desmin. Our in vitro studies using a rapid assembly protocol, C-terminally truncated desmin and two disease-causing mutants (I451M and R454W) suggest that CRYAB is a sensor for the surface topology of the desmin filament. Our data also suggest that CRYAB performs an assembly chaperone role because the assembling filaments have different CRYAB-binding properties during the maturation process. We suggest that the capability of CRYAB to distinguish between filaments with different surface topologies due either to mutation (R454W) or assembly protocol is important to understanding the pathomechanism(s) of desmin-CRYAB myopathies.

The specificity of the interaction between αB-crystallin and desmin filaments and its impact on filament aggregation and cell viability.

CRYAB (αB-crystallin) is expressed in many tissues and yet the R120G mutation in CRYAB causes tissue-specific pathologies, namely cardiomyopathy and cataract. Here, we present evidence to demonstrate that there is a specific functional interaction of CRYAB with desmin intermediate filaments that predisposes myocytes to disease caused by the R120G mutation. We use a variety of biochemical and biophysical techniques to show that plant, animal and ascidian small heat-shock proteins (sHSPs) can interact with intermediate filaments. Nevertheless, the mutation R120G in CRYAB does specifically change that interaction when compared with equivalent substitutions in HSP27 (R140G) and into the Caenorhabditis elegans HSP16.2 (R95G). By transient transfection, we show that R120G CRYAB specifically promotes intermediate filament aggregation in MCF7 cells. The transient transfection of R120G CRYAB alone has no significant effect upon cell viability, although bundling of the endogenous intermediate filament network occurs and the mitochondria are concentrated into the perinuclear region. The combination of R120G CRYAB co-transfected with wild-type desmin, however, causes a significant reduction in cell viability. Therefore, we suggest that while there is an innate ability of sHSPs to interact with and to bind to intermediate filaments, it is the specific combination of desmin and CRYAB that compromises cell viability and this is potentially the key to the muscle pathology caused by the R120G CRYAB.

Blurring the boundaries of space: shaping nursing lives at the Red Cross outposts in Ontario, 1922-1945.

Historians and other scholars interested in the history of hospitals have investigated the links between medical architecture and the organization of space with the evolution of modern medicine. The transformation over time in the architectural for of medical institutions has tended to reflect developments in medical science and therapeutic efficiency as well as elements in the broader social climate. Some authors, however, have argued for the agency of structure and spatial organization, to consider that they are not just containers with which human activities take place, but which also actively construct or constitute social practices and relations. Most studies of this nature have centred on large medical buildings especially in urban areas, and have examined the impact of architectural arrangement in relation to administrators and architects, physicians and patients. Fe have considered the interconnections of form and space with nurses, despite the prominence of institutional nursing labour since the late 19th-century. The following discussion begins an exploration of these concepts within the rural environment. Between 1922 and 1984, the Ontario Division of the Canadian Red Cross Society administered an outpost program in which it operated small hospitals and nursing stations in isolated communities throughout the northern reaches of the province. This article will focus primarily o n the one-nurse stations that the Division managed during the interwar years and the nurses that it hired to staff them. The interior spatial organization of these outposts, which led in particular to their multiple functions as tiny hospitals, community health centres and nurses' homes, not only shaped both the professional practice and the social or private lives of the Red Cross nurses but also contributed to the diffusion of contemporary precepts in health and medical care throughout a remote population.