RESUMO
To begin to characterize the components of the 20S proteasome of Giardia lamblia, we have cloned a genomic sequence encoding an alpha-chain (type alpha3/C9, predicted size 244 amino acid residues). Southern analysis indicated that a single gene codes for this protein, and a Northern blot exhibited a single signal at 850 nt. An antiserum against a C-terminal fragment of the alpha-chain expressed in Escherichia coli reacted with a single protein band of Mr 27,000 that was present at constant levels in trophozoites and encysting cells. On a 2D blot of the purified 20S proteasome, we identified the cross-reacting component as a single protein of IEP 6.0, in agreement with the IEP predicted by the coding sequence. Our data confirm that the G. lamblia 20S proteasome is typically eukaryotic in containing a set of diverged alpha-subunits.
Assuntos
Cisteína Endopeptidases/química , Cisteína Endopeptidases/genética , Giardia lamblia/genética , Complexos Multienzimáticos/química , Complexos Multienzimáticos/genética , Sequência de Aminoácidos , Animais , Southern Blotting , Cisteína Endopeptidases/metabolismo , Giardia lamblia/enzimologia , Dados de Sequência Molecular , Complexos Multienzimáticos/metabolismo , Complexo de Endopeptidases do Proteassoma , Proteínas Recombinantes/química , Proteínas Recombinantes/metabolismo , Transcrição GênicaAssuntos
Cisteína Endopeptidases/química , Cisteína Endopeptidases/isolamento & purificação , Giardia lamblia/enzimologia , Complexos Multienzimáticos/química , Complexos Multienzimáticos/isolamento & purificação , Animais , Eletroforese em Gel Bidimensional , Eletroforese em Gel de Poliacrilamida , Microscopia Eletrônica , Complexo de Endopeptidases do ProteassomaRESUMO
Laparoscopic gastric banding has become a common procedure in bariatric surgery. Early as well as late complications are, in comparison to conventional techniques, rare. Complications arising from the operative technique are: perforation, early pouch dilatation, gastric slippage, infections of the port and the band, erosion and defects of the band tube. Insufficient weight loss and late pouch dilatation arise from unsatisfactory compliance on the part of the patient. In 7.5% of 146 cases, reoperations were necessary. Obstruction of the pouch stoma and a slippage resulted in total food intolerance. After radiologic diagnosis the band system should be deflated first and a naso-gastric tube applied. If this does not result in the pouch collapsing, a revision operation is needed.
