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1.
Arch Virol ; 165(1): 157-167, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31748876

RESUMO

Endogenous retroviruses of domestic cats (ERV-DCs) are members of the genus Gammaretrovirus that infect domestic cats (Felis silvestris catus). Uniquely, domestic cats harbor replication-competent proviruses such as ERV-DC10 (ERV-DC18) and ERV-DC14 (xenotropic and nonecotropic viruses, respectively). The purpose of this study was to assess invasion by two distinct infectious ERV-DCs, ERV-DC10 and ERV-DC14, in domestic cats. Of a total sample of 1646 cats, 568 animals (34.5%) were positive for ERV-DC10 (heterozygous: 377; homozygous: 191), 68 animals (4.1%) were positive for ERV-DC14 (heterozygous: 67; homozygous: 1), and 10 animals (0.6%) were positive for both ERV-DC10 and ERV-DC14. ERV-DC10 and ERV-DC14 were detected in domestic cats in Japan as well as in Tanzania, Sri Lanka, Vietnam, South Korea and Spain. Breeding cats, including Singapura, Norwegian Forest and Ragdoll cats, showed high frequencies of ERV-DC10 (60-100%). By contrast, ERV-DC14 was detected at low frequency in breeding cats. Our results suggest that ERV-DC10 is widely distributed while ERV-DC14 is maintained in a minor population of cats. Thus, ERV-DC10 and ERV-DC14 have invaded cat populations independently.


Assuntos
Gammaretrovirus/classificação , Técnicas de Genotipagem/métodos , Infecções por Retroviridae/epidemiologia , Animais , Animais Domésticos , Ásia , Cruzamento , Gatos , Gammaretrovirus/genética , Gammaretrovirus/isolamento & purificação , Noruega , Filogenia , Filogeografia , Infecções por Retroviridae/virologia , Espanha , Tanzânia
2.
Arch Virol ; 163(4): 1073-1077, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29353423

RESUMO

Feline lymphomas are associated with the transduction and activation of cellular proto-oncogenes, such as c-myc, by feline leukemia virus (FeLV). We describe a polymerase chain reaction assay for detection of myc transduction usable in clinical diagnosis. The assay targets c-myc exons 2 and 3, which together result in a FeLV-specific fusion gene following c-myc transduction. When this assay was conducted on FeLV-infected feline tissues submitted for clinical diagnosis of tumors, myc transduction was detected in 14% of T-cell lymphoma/leukemias. This newly established system could become a useful diagnostic tool in veterinary medicine.


Assuntos
DNA Viral/genética , Vírus da Leucemia Felina/genética , Leucemia Felina/diagnóstico , Linfoma de Células T/diagnóstico , Reação em Cadeia da Polimerase/métodos , Proteínas Proto-Oncogênicas c-myc/genética , Animais , Linfócitos B/metabolismo , Linfócitos B/patologia , Gatos , Feminino , Expressão Gênica , Vírus da Leucemia Felina/isolamento & purificação , Leucemia Felina/genética , Leucemia Felina/metabolismo , Leucemia Felina/patologia , Linfoma de Células T/genética , Linfoma de Células T/metabolismo , Linfoma de Células T/patologia , Masculino , Proteínas de Fusão Oncogênica/genética , Proteínas de Fusão Oncogênica/metabolismo , Proteínas Proto-Oncogênicas c-myc/metabolismo , Linfócitos T/metabolismo , Linfócitos T/patologia , Transdução Genética
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