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1.
J Dairy Sci ; 98(7): 4560-71, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25935241

RESUMO

Assessment of lameness prevalence and severity requires visual evaluation of thelocomotion of a cow. Welfare schemes including locomotion assessments are increasingly being adopted, and more farmers and their veterinarians might implement a locomotion-scoring routine together. However, high within-observer agreement is a prerequisite for obtaining valid mobility scorings, and within-observer agreement cannot be estimated in a barn, because the gait of cows is dynamic and may change between 2 occasions. The objective of this study was to estimate the within-observer agreement according to the observers' educational background and experience with cattle, based on video recordings with very diverse types of gait. Groups of farmers, bovine veterinarians, first- and fourth-year veterinary students, researchers, and cattle-inexperienced sensory assessors evaluated mobility using a 5-point mobility score system developed specifically for walking cows (n=102 observers). The evaluation sessions were similar for all groups, lasted 75 min, and were organized as follows: introduction, test A, short training session, break, and test B. In total, video recordings of 22 cows were displayed twice in a random order (11 cows in each test × 2 replicates). Data were analyzed applying kappa coefficient, logistic regression, and testing for random effects of observers. The crude estimates of 95% confidence interval for weighted kappa in test A and B ranged, respectively, from 0.76 to 0.80 and 0.70 to 0.75. When adjusting for the fixed effects of video sample and gait scoring preferences, the probability of assigning the same mobility score twice to the same cow varied from 55% (sensory assessors) to 72% (fourth-year veterinary students). The random effect of the individual observers was negligible. That is, in general observers could categorize the mobility characteristics of cows quite well. Observers who preferred to assess the attributes back arch or the overall mobility score (based on uneven gait) had the highest agreement, respectively, 69 or 68%. The training session seemed insufficient to improve agreement. Nonetheless, even novice observers were able to achieve perfect agreement up to 60% of the 22 scorings with merely the experience obtained during the study (introduction and training session). The relatively small differences between groups, together with a high agreement, demonstrate that the new system is easy to follow compared with previously described scoring systems. The mobility score achieves sufficiently high within-observer repeatability to allow between-observer agreement estimates, which are reliable compared with other more-complex scoring systems. Consequently, the new scoring scale seems feasible for on-farm applications as a tool to monitor mobility within and between cows, for communication between farmers and veterinarians with diverse educational background, and for lamenessbenchmarking of herds.


Assuntos
Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/epidemiologia , Coxeadura Animal/diagnóstico , Variações Dependentes do Observador , Gravação em Vídeo , Adulto , Animais , Bovinos , Feminino , Marcha , Humanos , Modelos Logísticos , Masculino , Prevalência , Inquéritos e Questionários , Adulto Jovem
2.
J Dairy Sci ; 97(12): 7476-86, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25282423

RESUMO

Lameness causes decreased animal welfare and leads to higher production costs. This study explored data from an automatic milking system (AMS) to model on-farm gait scoring from a commercial farm. A total of 88 cows were gait scored once per week, for 2 5-wk periods. Eighty variables retrieved from AMS were summarized week-wise and used to predict 2 defined classes: nonlame and clinically lame cows. Variables were represented with 2 transformations of the week summarized variables, using 2-wk data blocks before gait scoring, totaling 320 variables (2 × 2 × 80). The reference gait scoring error was estimated in the first week of the study and was, on average, 15%. Two partial least squares discriminant analysis models were fitted to parity 1 and parity 2 groups, respectively, to assign the lameness class according to the predicted probability of being lame (score 3 or 4/4) or not lame (score 1/4). Both models achieved sensitivity and specificity values around 80%, both in calibration and cross-validation. At the optimum values in the receiver operating characteristic curve, the false-positive rate was 28% in the parity 1 model, whereas in the parity 2 model it was about half (16%), which makes it more suitable for practical application; the model error rates were, 23 and 19%, respectively. Based on data registered automatically from one AMS farm, we were able to discriminate nonlame and lame cows, where partial least squares discriminant analysis achieved similar performance to the reference method.


Assuntos
Comportamento Animal/fisiologia , Doenças dos Bovinos/diagnóstico , Lactação/fisiologia , Coxeadura Animal/diagnóstico , Animais , Automação , Bovinos , Doenças dos Bovinos/fisiopatologia , Indústria de Laticínios , Análise Discriminante , Feminino , Marcha , Coxeadura Animal/fisiopatologia , Análise dos Mínimos Quadrados , Leite/metabolismo , Monitorização Fisiológica/veterinária , Paridade , Gravidez , Sensibilidade e Especificidade
3.
J Dairy Sci ; 97(1): 552-61, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24239087

RESUMO

Evaluating the effects of all interventions in a dairy herd, including the effects of various herd health management programs (HHMP), is highly relevant. A traditional randomized controlled trial is the gold standard but is likely practically impossible or prohibitively expensive to use for a general evaluation of a HHMP. Generalizability may also be poor because of the dynamics of the production contexts. In this study, we demonstrate an approach for evaluating the effects of an HHMP in the field, specifying an intervention theory for an ongoing HHMP in the context of the Danish dairy industry. As an example, we suggest one coherent analytical approach for studying the possible effects on milk production of systematic postpartum examinations of vaginal discharge, which is supposed to improve detection and treatment of metritis or endometritis. This routine is one component of the HHMP. The data consisted of 121 herds and 76,953 lactations over a 15-yr period. For parity group 1, the negative effects of metritis (despite treatment) on 305-d milk production after a normal calving were reduced by 116 kg of energy-corrected milk after enrollment in the HHMP. For parity group 2 and parity group >2, enrollment in the HHMP resulted in a 129-kg and an 80-kg energy-corrected milk yield increase in milk production, respectively. The results indicate that effects of the HHMP existed, which were mediated through improved metritis detection. This study demonstrates the importance of a clear-cut intervention theory, although even with a theory, the research question can be too herd and context specific. In such a case, a within-herd randomized controlled trial study design seems to be the only way to achieve a valid result for a given herd, and acquiring valid results from an observational multi-herd study will be very difficult.


Assuntos
Criação de Animais Domésticos/métodos , Doenças dos Bovinos/prevenção & controle , Endometrite/veterinária , Animais , Bovinos , Indústria de Laticínios , Endometrite/prevenção & controle , Feminino , Lactação , Paridade , Gravidez
4.
J Dairy Sci ; 94(5): 2360-7, 2011 May.
Artigo em Inglês | MEDLINE | ID: mdl-21524525

RESUMO

In this study, 3 commonly used tests to diagnose ketosis were evaluated with a latent class model to avoid the assumption of an available perfect test. The 3 tests were the KetoLac BHB (Sanwa Kagaku Kenkyusho Co. Ltd., Nagoya, Japan) test strip that tests milk for ß-hydroxybutyrate, the KetoStix (Bayer Diagnostics Europe Ltd., Dublin, Ireland) test strip that tests urine for acetoacetate, and the fat-to-protein percentage ratio (FPR) in milk. A total of 8,902 cows were included in the analysis. The cows were considered to be a random sample from the population of Danish dairy cattle under intensive management, thus representing a natural spectrum of ketosis as a disease. All cows had a recorded FPR between 7 and 21 d postpartum. The KetoLac BHB recordings were available from 2,257 cows and 6,645 cows had a KetoStix recording. The recordings were analyzed with a modified Hui-Walter model, in a Bayesian framework. The specificity of the KetoLac BHB test and the KetoStix test were both high [0.99 (0.97-0.99)], whereas the specificity of FPR was somewhat lower [0.79 (0.77-0.81)]. The best sensitivity was for the KetoStix test [0.78 (0.55-0.98)], followed by the FPR [0.63 (0.58-0.71)] and KetoLac BHB test [0.58 (0.35-0.93)].


Assuntos
Acetoacetatos/urina , Doenças dos Bovinos/diagnóstico , Cetose/veterinária , Leite/química , Kit de Reagentes para Diagnóstico/veterinária , Ácido 3-Hidroxibutírico/análise , Animais , Bovinos , Doenças dos Bovinos/urina , Gorduras na Dieta/análise , Feminino , Cetose/diagnóstico , Cetose/urina , Proteínas do Leite/análise , Sensibilidade e Especificidade
5.
J Dairy Sci ; 92(1): 149-55, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19109273

RESUMO

Infection with Mycobacterium avium ssp. paratuberculosis (MAP) in dairy cattle often results in reduced milk production and premature culling. Some test-positive animals can live for years without being affected by infection, whereas others are test negative when they die from the infection. Our objective was to describe the deviation in milk production of cows with various MAP antibody profiles compared with their repeatedly test-negative herdmates in the same parity. Data were obtained from herds participating in the Danish control program on paratuberculosis, for which 4 annual MAP antibody ELISA of individual cows were performed per herd per year. A total of 136,489 ELISA results from 38,998 dairy cows in 64 herds were used along with 484,285 test-day records on energy-corrected milk (ECM) yield. Cows were divided into 6 antibody groups based on their repeated milk ELISA results: A0) repeated ELISA negative; A1) ELISA negative, but only once; A2) ELISA negative on the last 3 tests, but with 1 previous positive result; A3) ELISA negative on the last test, but with 1 or more previous positive results; A4) last sample was ELISA positive, but all previous were negative; and A5) at least the last 2 samples were ELISA positive. The expected test-day kilograms of ECM by herd and parity were estimated for cows in antibody group A0. Deviations from expected milk production were then assessed for cows in the other antibody groups relative to the time of the first test-positive ELISA result (D 0). Cows in groups A2, A3, and A5 produced approximately 0.5 kg of ECM/d more than cows in group A0 at 300 d before D 0. Cows in group A4 had a decline in milk production from d 300 before D 0, with daily milk production reduced by 5 kg of ECM at 200 d after D 0. Milk production of cows in group A5 was reduced by 2.5 kg of ECM at 300 d after D 0 compared with 300 d before D 0, whereas cows in groups A2 and A3 produced 0.5 kg of ECM more than cows in group A0. The conclusions of the study were that 1) increasing the number ELISA tests increases the predictive value of ELISA for inference on milk production losses, 2) a combination of ELISA with assessment of observed milk production may be a valuable tool for decisions on culling, and 3) the declines in milk production attributable to MAP occurred over a long time period, and may not be realized by the herd manager without more advanced management tools such as the model proposed here.


Assuntos
Anticorpos Antibacterianos/sangue , Doenças dos Bovinos/imunologia , Lactação/imunologia , Leite/metabolismo , Paratuberculose/imunologia , Animais , Bovinos , Feminino , Fatores de Tempo
6.
Vet Res Commun ; 33(2): 123-37, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18686006

RESUMO

The disease risk is very high among transition cows that may suffer from poor appetite. The aim of the present study was therefore to investigate the effect of drenching on energy balance, hydration state and selected production parameters in fresh cows. Twenty-one Danish Holstein-Friesian dairy cows in late pregnancy were randomly allocated to either treatment (TG) or control group (CG). TG cows were drenched twice with 20 l of water containing a mixture of calcium propionate, MgSO(4), and KCL specifically developed for prophylactic treatment of fresh cows. The results indicated that cows become dehydrated around calving, losing on average 53 l (TG) and 24.5 l (CG) of extra-cellular fluid, respectively. The drenching volume applied in the present study did not affect the degree of hydration after calving. Based on blood NEFA and BHB values it was shown that drenching caused a reduction in the degree of negative energy balance (NEB). Somatic cell count (SCC) for cows in first lactation was lower in the TG compared to CG. Milk yield was unaffected by treatment. We conclude that prophylactic drenching added little to the health promotion in the transition cows in the present study. Instead, increased focus on management routines would probably be of more value.


Assuntos
Bovinos/fisiologia , Metabolismo Energético/fisiologia , Período Pós-Parto/fisiologia , Água/administração & dosagem , Ácido 3-Hidroxibutírico/sangue , Animais , Temperatura Corporal/fisiologia , Desidratação/metabolismo , Desidratação/terapia , Ácidos Graxos não Esterificados/sangue , Feminino , Hematócrito/veterinária , Lactação , Sulfato de Magnésio/administração & dosagem , Leite/química , Leite/citologia , Leite/metabolismo , Cloreto de Potássio/administração & dosagem , Gravidez , Propionatos/administração & dosagem , Distribuição Aleatória , Análise de Regressão
7.
Vet Rec ; 163(3): 80-5, 2008 Jul 19.
Artigo em Inglês | MEDLINE | ID: mdl-18641376

RESUMO

In a cross-sectional study of 6240 Danish Holstein cows in 55 herds using loose-housing systems, sole haemorrhages and heel horn erosions occurred frequently in almost all the herds. Digital dermatitis occurred in 47 of the 55 herds (85 per cent). Spearman correlation coefficients revealed relationships between lameness and sole ulcer (0.36), between heel horn erosion and sole haemorrhage (0.39), between heel horn erosion and interdigital dermatitis (0.29) and between sole ulcer and double sole (0.26). The prevalence of heel horn erosion, sole haemorrhage, interdigital dermatitis and digital dermatitis appeared to be most affected by herd-level factors. The associations between individual foot trimmers and all the foot lesions were statistically significant. The risk of interdigital dermatitis increased with decreasing amounts of bedding. Cows housed throughout the year had a marginally higher risk of sole haemorrhage.


Assuntos
Doenças dos Bovinos/patologia , Doenças do Pé/veterinária , Criação de Animais Domésticos , Animais , Bovinos , Doenças dos Bovinos/epidemiologia , Estudos Transversais , Dinamarca/epidemiologia , Feminino , Doenças do Pé/epidemiologia , Doenças do Pé/patologia , Abrigo para Animais , Prevalência
8.
J Dairy Sci ; 91(5): 2089-97, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18420640

RESUMO

Lameness causes major financial losses and welfare problems in dairy herds. Prevention of foot lesions may suffice in the majority of lameness cases. The objectives of this longitudinal study were to describe the dynamics and associations between abnormal hind leg conformation, asymmetric claws, lameness, and foot lesions in 122 Danish Holstein heifers from an average of 41 d before first calving until dry off or culling. The cattle were housed either in a free-stall system with cubicles or in deep-bedded straw yard. The claws of all cattle were examined on up to 5 occasions. The associations between foot lesions, lameness, symmetry of the claws, shape of the dorsal toe-wall, and the conformation of the hind legs were examined statistically using mixed models. A large proportion (81%) of the heifers had cow-hocked conformation, with wide-based stance, hocks together, and lateral rotation of the foot before calving, and 25% of the heifers had locomotion scores above 2, indicating signs of lameness, before calving. Our results indicate that lameness, abnormal conformation, and lesions acquired precalving persist throughout the first lactation. Thinner cows were more cow-hocked, and cow-hocked cows had a higher frequency of sole hemorrhages. Increased severity of white line lesion was associated with greater claw asymmetry. More severe lameness and sole hemorrhages were found in symmetric claws.


Assuntos
Doenças dos Bovinos/etiologia , Membro Posterior/anormalidades , Coxeadura Animal/etiologia , Animais , Bovinos , Dinamarca , Feminino , Doenças do Pé/etiologia , Doenças do Pé/veterinária , Casco e Garras/anormalidades , Modelos Lineares , Estudos Longitudinais , Estudos Prospectivos , Reprodutibilidade dos Testes
9.
J Dairy Sci ; 91(2): 620-31, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18218749

RESUMO

Monte Carlo simulation was used to predict the long-term financial performance related to the technical performance of dairy herds. The indicators addressed were derived from data collected routinely in the herd. They indicated technical performance that can be affected by the farmer or the consultant, and they were derived from expected cause-effect relations between technical performance and financial performance at the herd level. The study included the indicators shape of lactation curve, reproduction efficiency, heifer management, variation between cows in lactation curve persistency, mortality in cows and calves, dynamics of body condition, and somatic cell counts. Each indicator was defined by 2 or 3 levels, and 2- and 3-factor interactions were included in the simulation experiment, which included 72 scenarios. Each scenario was replicated 200 times, and the resulting gross margin per cow was analyzed as the measure of financial performance. The potential effects of the selected indicators on the gross margin were estimated by means of an ANOVA. The final model allowed estimation of the financial value of specific changes within the key performance indicators. This study indicated that improving the shape of the herd-level lactation curve by 1 quartile was associated with an increase in gross margin of euro 227 per cow year. This represents 53% of the additional available gross margin associated with all the management changes included in the study. The improved herd-level lactation curve increased the gross margin 2.6 times more than improved reproduction efficiency, which again increased the gross margin 2.6 to 5.9 times more than improved management related to heifers, body condition score, mortality, and somatic cell counts. These results were implemented in a simple "metamodel" that used data extracted from ordinary management software to predict herd-specific financial performance related to major management changes. The metamodel was derived from systematic experiments with a complex simulation model that was used directly for advanced herd-specific decision support. We demonstrated the use of these key performance indicators to forecast the financial consequences of different "what-if" herd management options, with emphasis on herd health economics.


Assuntos
Bovinos , Indústria de Laticínios/economia , Leite/economia , Modelos Econômicos , Animais , Simulação por Computador , Dinamarca , Feminino , Método de Monte Carlo , Reprodução
10.
Acta Vet Scand ; 48: 15, 2006 Aug 22.
Artigo em Inglês | MEDLINE | ID: mdl-16987394

RESUMO

A cross-sectional observational study with repeated observations was conducted on 16 Danish dairy farms to quantify the influence of observer, parity, time (stage in lactation) and farm on variables routinely selected for inclusion in clinical protocols, thereby to enable a more valid comparison of udder health between different herds. During 12 months, participating herds were visited 5 times by project technicians, who examined 20 cows and scored the selected clinical variables. The estimates of effect on variables were derived from a random regression model procedure. Statistical analyses revealed that, although estimates for occurrence of several the variables, e.g. degree of oedema, varied significantly between observers, the effects on many of these estimates were similar in size. Almost all estimates for occurrences of variables were significantly affected either parity and lactation stage, or by both e.g. udder tissue consistency. Some variables, e.g. mange, had high estimates for the farm component, and others e.g. teat skin quality had a high individual component. Several of the variables, e.g. wounds on warts, had a high residual component indicating that a there still was a major part of the variation in data, which was unexplained. It was concluded that most of the variables were relevant for implementation in herd health management, but that adjustments need to be made to improve reliability.


Assuntos
Indicadores Básicos de Saúde , Glândulas Mamárias Animais/fisiologia , Variações Dependentes do Observador , Animais , Bovinos , Estudos Transversais , Indústria de Laticínios , Feminino , Humanos , Lactação/fisiologia , Paridade/fisiologia , Gravidez
11.
J Dairy Sci ; 89(9): 3721-8, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16899709

RESUMO

Body condition scores (BCS) are very useful for dairy herd management and breeding programs, but the consistency and quality of recordings made by consultants in the field are unknown. The objectives of this study were 1) to estimate the agreement in BCS within and among practicing dairy veterinarians and 2) to provide an indication of the effects of training and the value of calibration, and of what efforts need to be made to obtain a validity and precision in BCS adequate for management purposes. A total of 2,230 scores were recorded by 51 practicing dairy veterinarians and 6 highly trained instructors. The 6 instructors were cross-trained to validate calibration consistency in assigning BCS. Each individual scored approximately 20 cows twice, with the second scoring occurring approximately 2.5 h after the first. Between the 2 recordings, the respective instructors conducted a training session for the practicing veterinarians using other cows. A weighted kappa coefficient was used to assess agreement among and within classifiers. Excellent agreement (kappa > or = 0.86) was documented between repeated BCS recorded for the same cows by the highly trained instructors. In addition, the BCS provided by multiple classifiers from the instructor team appeared to be comparable across herds and classifiers. This legitimizes the use of BCS for benchmarking at both the cow and the herd level. The within-classifier and between-classifier kappa values were in the ranges of 0.22 to 0.75 and 0.17 to 0.78, respectively, in the group of practicing dairy veterinarians. Many of the veterinarians provided estimates of average BCS that differed considerably from the BCS recorded by the instructors. Between-classifier comparisons of herd BCS are not warranted unless a validation has been performed. If scores are collected by multiple classifiers with varying experience, a valid but imprecise estimate of the true population mean of BCS may be obtained if classifiers are inexperienced. The limited training effort used in this study seemed to have brought about substantial improvement in the validity and precision of the BCS determined by practicing veterinarians, compared with the BCS recorded on the same cows by highly trained classifiers.


Assuntos
Bovinos/fisiologia , Indústria de Laticínios/métodos , Nível de Saúde , Variações Dependentes do Observador , Animais , Constituição Corporal , Dinamarca , Feminino , Humanos , Reprodutibilidade dos Testes
13.
J Dairy Sci ; 88(1): 128-36, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15591375

RESUMO

Milk leakage in dairy cows is a symptom of impaired teat sphincter function. Milk leakage is related to an increased risk of mastitis in heifers and cows, and causes hygiene problems. The aim of our study was to assess whether teat shape, condition of teat orifice, and peak milk flow rate are risk factors for milk leakage. We conducted a longitudinal observational study in 15 German dairy farms in which cows were maintained in loose housing. The farms were visited monthly at 2 consecutive milkings. During the evening milking, milk flow curves were measured with the LactoCorder. Milk leakage was recorded during the subsequent morning milking, when cows entered the milking parlor. Immediately after detachment of the milking cluster, teat shape, teat end shape, and condition of the teat orifice of cows were assessed between 9 and 100 d in milk (DIM) and during late lactation (>250 DIM). Data from 1600 cows were analyzed. Milk leakage was treated as the binary response variable in a logistic regression model with herd as a random effect. Primiparous cows with high peak milk flow and teat canal protrusion were at greater risk of milk leakage. High peak milk flow rate, short teats, teat canal protrusion, inverted teat ends, and early lactation increased the risk of milk leakage in multiparous cows. Random herd effects accounted for only 10% of the total variation, indicating that the impact of management or other herd-level factors on the occurrence of milk leakage is virtually negligible for practical purposes.


Assuntos
Doenças dos Bovinos/etiologia , Lactação , Glândulas Mamárias Animais , Animais , Bovinos , Doenças dos Bovinos/patologia , Doenças dos Bovinos/fisiopatologia , Feminino , Alemanha , Modelos Logísticos , Estudos Longitudinais , Glândulas Mamárias Animais/patologia , Glândulas Mamárias Animais/fisiopatologia , Mastite Bovina/etiologia , Fatores de Risco
14.
J Dairy Sci ; 87(5): 1217-28, 2004 May.
Artigo em Inglês | MEDLINE | ID: mdl-15290970

RESUMO

A cross-sectional study was conducted to explore the applicability of systematic clinical examinations of udders as an additional tool for the evaluation of udder health status on dairy farms. During 2000, each of the 16 dairy farms was visited 5 times; 20 cows per farm were chosen at random at each visit for clinical udder examination immediately after milking. The clinical examination included both pathological and morphological variables. One examination per cow was included in the analysis (n = 707 cows). Principal component analysis (PCA) was performed in 3 steps. First, 19 variables characterizing udder and teats were analyzed (PCA 1). Second, the variables parity and stage of lactation were included (PCA 2). Finally, somatic cell count (SCC) and milk yield (PCA 3) were included. The PCA resulted in 4 components that explained 30% of the variation of the data: 1) small udder, 2) distressed udder, 3) mastitis udder, and 4) soiled udder. Variables with high positive correlation to the "small udder" were small udder shape, short teats, and first parity. Impaired teat surface, hard udder texture, and a long udder shape were related to the "distressed udder." The "mastitis udder" was characterized by the clinical variables asymmetry between front quarters, asymmetry between hind quarters, knotty tissue, and acute clinical mastitis. Reduced milk yield and high SCC were related to the "mastitis udder," whereas low SCC was related to the "small udder." The "soiled udder" was related to early lactation. Including this information in the assessment of udder health may be of substantial value for data analysis in farms with suspected under-reporting of clinical mastitis.


Assuntos
Bovinos , Glândulas Mamárias Animais , Exame Físico/veterinária , Animais , Contagem de Células , Dinamarca , Feminino , Nível de Saúde , Lactação , Glândulas Mamárias Animais/patologia , Mastite Bovina/diagnóstico , Leite/citologia , Paridade
15.
J Dairy Sci ; 86(10): 3174-83, 2003 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-14594237

RESUMO

Production and disease data from 17,488 lactations in 48 Danish organic dairy herds from 1997 to 2001 were analyzed to obtain estimates on the effect of somatic cell counts (SCC) and mastitis treatment on milk production. A multilevel three-parameter piecewise random coefficients linear model with energy-corrected milk (ECM) as dependent variable and herd, lactation, and test days as levels, was used to model the lactation curve. Covariates related to production, SCC, veterinary treatments, and reproductive performance in the previous lactation as well as information on other diseases in the current lactation were included to describe the production capacity of the individual cow. The average daily milk production at herd level was 20.8, 24.2, and 25.8 kg of ECM/d in first, second, and third or later lactation. The estimates for production losses were on average 0.2, 0.3, and 0.4 kg of ECM/d in first, second, and third or later lactation with each twofold increase in SCC between 100,000 and 1,500,000 cells/ml. The effect varied with the stage of lactation and was nonsignificant around 60 d postpartum and highest at the end of the lactation. The production losses in cows treated for mastitis varied with parity and stage of lactation and were modified by the SCC after treatment. For a cow in third lactation with a SCC below 100,000 cells/ ml before treatment at days in milk = 15, the predicted loss was 435 kg of ECM, including a loss of 135 kg of ECM because of higher SCC compared with the level before treatment. Most of the variation in production related to SCC and mastitis was at the lactation level, and no significant differences were found between herds grouped according to milk production level, SCC, or prevalence of mastitis treatment.


Assuntos
Contagem de Células , Lactação , Mastite Bovina/tratamento farmacológico , Leite/citologia , Animais , Bovinos , Indústria de Laticínios , Feminino , Alimentos Orgânicos , Mastite Bovina/fisiopatologia , Paridade
16.
J Dairy Sci ; 85(11): 2795-802, 2002 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-12487446

RESUMO

A longitudinal study was performed to determine the course of the milk antibody response in cows presumably infected with Mycobacterium avium subsp. paratuberculosis. Milk samples were collected repeatedly (1 to 10 times) from all lactating cows in seven Danish dairy herds. A total of 4,289 observations from 812 cows was analyzed after exclusion of samples collected after 280 days in milk (DIM). The level of antibodies in the milk samples was assessed using an indirect ELISA. A piece-wise linear random coefficient regression model was specified. The model controlled for the effect of herd, breed, laboratory effects, and age at first calving to estimate parity-specific antibody responses in relation to DIM. Separate antibody profiles were estimated for fecal culture-positive and fecal culture-negative cows. The resulting population average models showed higher antibody levels for fecal culture-positive cows and higher antibody levels with increasing parity. On average, the antibody response was high at the beginning and end of lactation. However, evaluating the cows individually indicated that most cows actually had quite stable ELISA levels throughout lactation, with some cows having higher levels than others. Thus, two criteria seem applicable to assess whether a cow is infected: stability and ELISA level. The random coefficients for each cow were highly significant. Thus, the study suggests that all cows can be classified into one of the four categories by combining the cow-level ELISA characteristics "stability" and "level" as an aid in the diagnosis ofparatuberculosis and thereby substantially increasing the sensitivity of the ELISA.


Assuntos
Anticorpos Antibacterianos/biossíntese , Doenças dos Bovinos/imunologia , Leite/imunologia , Mycobacterium avium subsp. paratuberculosis/imunologia , Paratuberculose/imunologia , Animais , Bovinos , Doenças dos Bovinos/diagnóstico , Ensaio de Imunoadsorção Enzimática/veterinária , Fezes/microbiologia , Feminino , Lactação/imunologia , Modelos Lineares , Estudos Longitudinais , Leite/microbiologia , Paratuberculose/diagnóstico , Paridade/imunologia , Sensibilidade e Especificidade
17.
Acta Vet Scand ; 43(3): 173-84, 2002.
Artigo em Inglês | MEDLINE | ID: mdl-12564547

RESUMO

This study examined the possibilities of using clinical parameters related to the bovine udder for characterisation of udder health. Five clinicians performed systematic clinical recordings of udder health at 3 visits to 4 dairy herds. Several of the clinical parameters were scored on an ordinal scale. The agreement between clinicians was compared using kappa statistics. Factor analysis was used to identify udder types. The clinical evaluations showed substantial variation among clinicians. Parameters that were not directly related to pathological conditions showed the highest variation e.g. length of the claws, teat shape and hardness of the udder parenchyma. On the other hand, evaluation of pathological parameters such as nodes in the udder, skin lesions and oedema showed good agreement between clinicians. Udder types identified by means of factor analysis were found to be suitable for characterisation of udder health. Especially one factor related to dry quarters and udder asymmetry showed a more consistent relationship to milk yield than traditionally applied udder health parameters such as treatment rate and cell count. It is concluded that there is a considerable need for increased efforts among clinicians in order to standardise clinical recordings. It is further concluded that certain combinations of extended clinical recordings have significant perspectives for future characterisation of udder health.


Assuntos
Indústria de Laticínios , Glândulas Mamárias Animais/patologia , Animais , Bovinos , Dinamarca , Análise Fatorial , Feminino , Nível de Saúde
18.
Vet Microbiol ; 71(1-2): 89-101, 2000 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-10665537

RESUMO

The aim of the present study was to examine the diversity of Staphylococcus aureus isolates from bovine intramammary infections (IMI) in nine dairy herds, and compare these with isolates from other sites on the cows by phage- and ribotyping. Whether colonisation of milkers with S. aureus could be a source of infection for bovine IMI was investigated. In addition, 100 epidemiologically unrelated S. aureus isolates from asymptomatic human carriers were also phage- and ribotyped to compare the human and bovine reservoir of S. aureus in Denmark. A total of 625 S. aureus isolates from bovine IMI, bovine skin lesions, milking personnel, and non-farm-related human carriers were included in the study. Certain types predominated in one or several herds during the study period of one-and-a-half to two years, whereas the presence of other types was of a more sporadic nature. Within the individual herds, there was a close correspondence between ribo- and phage types of S. aureus isolated from bovine intramammary infections and skin lesions. Isolates from milking personnel, however, were not identical to any of the predominant intramammary strains. Furthermore, several of the isolates from milking personnel showed ribo- and phage patterns identical to S. aureus isolates from human carriers. The findings of the present study underline the importance of strict milking hygiene and improvement of current mastitis therapy. The results support the hypothesis that some S. aureus mastitis strains are more contagious, virulent or persistent than others. The human reservoir of S. aureus does not play a major role as a source of bovine intramammary infections.


Assuntos
Mastite Bovina/microbiologia , Infecções Estafilocócicas/veterinária , Staphylococcus aureus/isolamento & purificação , Doenças dos Trabalhadores Agrícolas/microbiologia , Animais , Portador Sadio/microbiologia , Bovinos , Indústria de Laticínios , Dinamarca , Reservatórios de Doenças , Feminino , Humanos , Infecções Estafilocócicas/microbiologia
19.
J Dairy Res ; 65(2): 175-86, 1998 May.
Artigo em Inglês | MEDLINE | ID: mdl-9627837

RESUMO

Records of claw trimmings were analysed in seven organic and six conventional Danish herds (a total of 974 cows). The housing systems represented were tie stall systems, loose housing system with slatted floor (one organic herd), and deep litter systems (deep straw bedding). Occurrence of sole disorders was analysed separately for cows in first lactation and for cows in later lactations. Three different responses (acute haemorrhage, sole ulcer in one leg and sole ulcer in two or more legs) were analysed using three binomial logistic regression analyses for each group. Herd analysed as a fixed effect was a strong risk factor for all kinds of sole ulcer. Lactation stage was a risk factor for acute haemorrhage in both groups of cows, and for sole ulcer in first parity cows. In general, there was a strong positive association between the period 61-120 d post partum and the presence of sole disorders. Breed was associated with acute haemorrhage in cows in second and later parities, and sole ulcer in one leg only in first parity cows in an interaction with lactation stage in both conditions. Danish Friesian cows were strongly associated with sole disorder, although the combination of lactation stage from 61 to 120 d post partum in cows of other dual purpose breeds was positively associated with the presence of sole ulcer in one leg only in first parity cows. The time of year for claw trimming was a risk factor for acute haemorrhage in first parity cows, with the period from December to January most strongly associated with acute haemorrhage. Previous disease treatment was a risk factor for sole ulcer in two or more legs in second and later parities. Udder related disorders and disorders other than reproductive problems were positively associated with the occurrence of sole ulcer. Body weight at calving was associated with acute haemorrhage in cows in second and subsequent parities. Body weight lower than the mean herd level by > 50 kg was negatively associated with acute haemorrhage.


Assuntos
Criação de Animais Domésticos , Doenças dos Bovinos/epidemiologia , Doenças do Pé/veterinária , Casco e Garras , Abrigo para Animais , Análise de Variância , Animais , Bovinos , Doenças dos Bovinos/etiologia , Doenças dos Bovinos/prevenção & controle , Feminino , Doenças do Pé/epidemiologia , Doenças do Pé/prevenção & controle , Lactação , Análise de Regressão , Estações do Ano
20.
J Dairy Sci ; 80(9): 1988-95, 1997 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-9313139

RESUMO

The objective of this study was to evaluate the use of hip height and width, body condition score, and relevant demographic information to predict body weight (BW) of dairy cows. Seven regression models were developed from data from 972 observations of 554 cows. Parity, hip height, hip width, and body condition score were consistently associated with BW. The coefficients of multiple determination varied from 80 to 89%. The number of significant terms and the parameter estimates of the models differed markedly among groups of cows. Apparently, these differences were due to breed and feeding regimen. Results from this study indicate that a reliable model for estimating BW of very different dairy cows maintained in a wide range of environments can be developed using body condition score, demographic information, and measurements of hip height and hip width. However, for management purposes, substantial improvements can be obtained by developing models that are specific to a given site.


Assuntos
Constituição Corporal , Peso Corporal , Bovinos/fisiologia , Animais , Feminino , Modelos Biológicos
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