A study of antibody formation by Baptisia tinctoria T.M. in experimental animals

Injections of Baptisia tinctoria T.M. (Wild Indigo) were administered i.m. to two healthy rabbits six times at weekly intervals. Blood samples were collected before, during and at the conclusion of the trial. Blood was analysed for antibodies against Salmonella typhosa, using the Widal reaction. A total leucocyte count was also carried out. A third rabbit was kept as control and was injected only with normal saline i.m. Its blood samples were also collected according to the above schedule. No formation of antibodies against Salmonella typhosa was observed in any experimental animal or control whereas marked leucopenia was observed in both experimental rabbits