RESUMO
We report, in this article, a piperidin-4-one derivative carrying pyrenyl fluorescent reporter groups which acts as a Cd2+ ion sensor. The compound is synthesized and characterized using IR and NMR spectral techniques. The compound forms an inclusion complex with ß-cyclodextrin. It selectively binds to Cd2+ ions in water and aqueous ß-cyclodextrin media. The stoichiometry of the host-guest complex of the compound with ß-cyclodextrin is 1:2. The ligand-metal ion binding stoichiometry is 1:1 both in water and in ß-cyclodextrin. The linear concentration range of detection of the metal ion is reported. Cyclodextrin complex formation does not affect the metal ion selectivity of the compound.
Assuntos
Cádmio/análise , Corantes Fluorescentes/química , beta-Ciclodextrinas/química , Cádmio/metabolismo , Técnicas de Química Sintética , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/metabolismo , Espectroscopia de Ressonância Magnética , Piperidonas/química , Espectrofotometria Ultravioleta , Poluentes Químicos da Água/análiseRESUMO
Magnetic nanoparticles (MNPs) are intriguing due to their potency to deliver anti-cancer drugs. This paper presents the inference from our experimental attempts to add merit to the concept of magnetic drug carrier, by designing calcium ferrite nanoparticles and coating them with a biocompatible dextran tethered with a hydrophobic cavity-containing molecule, ß-cyclodextrin (ß-CD). The size, crystal system, and the morphology of the MNPs are studied. The magnetic properties are explored using vibrating sample magnetometry, SQUID and Mössbauer spectroscopy. The roughly 75nm MNPs, encapsulated with the ß-CD-dextran conjugate allows a slow and sustained in vitro release of the loaded anti-cancer drug, Camptothecin, from the polymer shell. The study of cytotoxicity reveals that the loaded Camptothecin retains its potency as efficient as an effective carrier of the anti-cancer drug. Further, the toxicity of the nanomaterial is tested on an organism which is highly sensitive to toxicity i.e., brine shrimp (Artemia salina). The polymer coating brings down the toxicity of the MNPs.
Assuntos
Compostos de Cálcio/química , Ciclodextrinas/química , Preparações de Ação Retardada/química , Compostos Férricos/química , Magnetismo , Nanopartículas/química , Polímeros/química , Animais , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/farmacocinética , Antineoplásicos Fitogênicos/farmacologia , Artemia/efeitos dos fármacos , Camptotecina/química , Camptotecina/farmacocinética , Camptotecina/farmacologia , Sobrevivência Celular/efeitos dos fármacos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacocinética , Materiais Revestidos Biocompatíveis/farmacologia , Preparações de Ação Retardada/farmacocinética , Preparações de Ação Retardada/farmacologia , Liberação Controlada de Fármacos , Células HeLa , HumanosRESUMO
Ion-induced change in fluorescence is a straight-forward method for detection of toxic metal ions showing immediate response. Cadmium ions are toxic to the environment. We report in this paper a piperidine-4-one-based fluorescent chemosensor of Cd2+ ions, designed and synthesized by a simple method. The compound is characterized using infra-red (IR) and 1 H-NMR spectral techniques. The chemosensor showed Cd2+ ion selectivity and sensitivity in aqueous solution. The stoichiometry and the binding constants were determined using fluorescence spectroscopy. Piperidine-4-one shows a 1:1 stoichiometric binding to Cd2+ . The limit of detection of Cd2+ was reported.
