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1.
Elife ; 92020 04 16.
Artigo em Inglês | MEDLINE | ID: mdl-32297861

RESUMO

In Wnt/ß-catenin signaling, the transcriptional coactivator ß-catenin is regulated by its phosphorylation in a complex that includes the scaffold protein Axin and associated kinases. Wnt binding to its coreceptors activates the cytosolic effector Dishevelled (Dvl), leading to the recruitment of Axin and the inhibition of ß-catenin phosphorylation. This process requires interaction of homologous DIX domains present in Dvl and Axin, but is mechanistically undefined. We show that Dvl DIX forms antiparallel, double-stranded oligomers in vitro, and that Dvl in cells forms oligomers typically <10 molecules at endogenous expression levels. Axin DIX (DAX) forms small single-stranded oligomers, but its self-association is stronger than that of DIX. DAX caps the ends of DIX oligomers, such that a DIX oligomer has at most four DAX binding sites. The relative affinities and stoichiometry of the DIX-DAX interaction provide a mechanism for efficient inhibition of ß-catenin phosphorylation upon Axin recruitment to the Wnt receptor complex.


Stem cells can give rise to many types of specialized cells through a process called differentiation, which is partly regulated by changes in the levels of a protein known as ß-catenin. On one hand, a 'destruction complex' can keep ß-catenin levels low; this complex includes a protein called Axin and an enzyme known as GSK-3, which can tag ß-catenin for degradation. On the other hand, when ß-catenin levels need to increase, another protein called Dishevelled is activated. By binding to Axin, Dishevelled can bring the destruction complex in contact with other proteins, which leads to the deactivation of GSK-3. Dishevelled and Axin interact via a region that is similar in the two proteins, called DIX in Dishevelled and DAX in Axin. Studies of DIX and DAX have shown that both regions can form polymers ­ that is, a high number of similar units can bind together to form larger structures. However, these experiments were at higher concentrations than would be found in the cell. It was thought that, when combined, DIX and DAX might form these long chains together, preventing Axin from carrying out its role in destroying ß-catenin. Kan et al. set out to better understand this process by studying how DIX and DAX behave separately, and how they interact. The proteins were examined using a technique called cryo-electron microscopy, which allows scientists to dissect the structure of large proteins. When there was a high concentration of DIX in the sample, the molecules attached to one another to form long double-stranded helices. Similarly, DAX also formed helices, but these were shorter and only single-stranded. When the two proteins were combined, DAX bound only to the ends of short DIX chains, so that there are not more than four DAX chains attached to each DIX double helix. To see if this behaviour happens naturally, Kan et al. attached fluorescent tags to Dishevelled proteins and followed them in living cells: this showed that Dishevelled forms smaller chains with fewer than ten molecules. Together these results highlight how Dishevelled binds to Axin to deactivate GSK-3, to prevent the enzyme from promoting ß-catenin destruction. Mutations in the genes that encode ß-catenin or its regulators are associated with cancer. Ultimately, a better understanding of how ß-catenin is regulated could help to identify new opportunities for drug development.


Assuntos
Proteína Axina/metabolismo , Diferenciação Celular/fisiologia , Proteínas Desgrenhadas/metabolismo , Via de Sinalização Wnt/fisiologia , Animais , Humanos , Camundongos
2.
J Biol Chem ; 290(4): 2175-88, 2015 Jan 23.
Artigo em Inglês | MEDLINE | ID: mdl-25492864

RESUMO

The fusion of intracellular membranes is driven by the formation of a highly stable four-helix bundle of SNARE proteins embedded in the vesicle and target membranes. N-Ethylmaleimide sensitive factor recycles SNAREs after fusion by binding to the SNARE complex through an adaptor protein, αSNAP, and using the energy of ATP hydrolysis to disassemble the complex. Although only a single molecule of αSNAP binds to a soluble form of the SNARE complex, we find that three molecules of αSNAP are used for SNARE complex disassembly. We describe an engineered αSNAP trimer that supports more efficient SNARE complex disassembly than monomeric αSNAP. Using the trimerized αSNAP, we find that N-ethylmaleimide-sensitive factor hydrolyzes 10 ATP molecules on average to disassemble a single SNARE complex.


Assuntos
Proteínas SNARE/química , Proteínas de Ligação a Fator Solúvel Sensível a N-Etilmaleimida/química , Trifosfato de Adenosina/química , Animais , Anisotropia , Sequência de Bases , Membrana Celular/metabolismo , Cricetulus , Escherichia coli/metabolismo , Hidrólise , Fusão de Membrana , Microscopia de Fluorescência , Dados de Sequência Molecular , Nucleotídeos/química , Ligação Proteica , Engenharia de Proteínas , Transporte Proteico
3.
Elife ; 3: e01998, 2014 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-24642411

RESUMO

Glycogen synthase kinase-3 (GSK-3) is a key regulator of many cellular signaling pathways. Unlike most kinases, GSK-3 is controlled by inhibition rather than by specific activation. In the insulin and several other signaling pathways, phosphorylation of a serine present in a conserved sequence near the amino terminus of GSK-3 generates an auto-inhibitory peptide. In contrast, Wnt/ß-catenin signal transduction requires phosphorylation of Ser/Pro rich sequences present in the Wnt co-receptors LRP5/6, and these motifs inhibit GSK-3 activity. We present crystal structures of GSK-3 bound to its phosphorylated N-terminus and to two of the phosphorylated LRP6 motifs. A conserved loop unique to GSK-3 undergoes a dramatic conformational change that clamps the bound pseudo-substrate peptides, and reveals the mechanism of primed substrate recognition. The structures rationalize target sequence preferences and suggest avenues for the design of inhibitors selective for a subset of pathways regulated by GSK-3. DOI: http://dx.doi.org/10.7554/eLife.01998.001.


Assuntos
Quinase 3 da Glicogênio Sintase/antagonistas & inibidores , Sequência de Aminoácidos , Animais , Catálise , Cristalografia por Raios X , Quinase 3 da Glicogênio Sintase/química , Quinase 3 da Glicogênio Sintase/metabolismo , Humanos , Dados de Sequência Molecular , Fosforilação , Conformação Proteica , Homologia de Sequência de Aminoácidos , Especificidade por Substrato
4.
Nature ; 502(7472): 575-579, 2013 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-24056936

RESUMO

G-protein-coupled receptors (GPCRs) are integral membrane proteins that have an essential role in human physiology, yet the molecular processes through which they bind to their endogenous agonists and activate effector proteins remain poorly understood. So far, it has not been possible to capture an active-state GPCR bound to its native neurotransmitter. Crystal structures of agonist-bound GPCRs have relied on the use of either exceptionally high-affinity agonists or receptor stabilization by mutagenesis. Many natural agonists such as adrenaline, which activates the ß2-adrenoceptor (ß2AR), bind with relatively low affinity, and they are often chemically unstable. Using directed evolution, we engineered a high-affinity camelid antibody fragment that stabilizes the active state of the ß2AR, and used this to obtain crystal structures of the activated receptor bound to multiple ligands. Here we present structures of the active-state human ß2AR bound to three chemically distinct agonists: the ultrahigh-affinity agonist BI167107, the high-affinity catecholamine agonist hydroxybenzyl isoproterenol, and the low-affinity endogenous agonist adrenaline. The crystal structures reveal a highly conserved overall ligand recognition and activation mode despite diverse ligand chemical structures and affinities that range from 100 nM to ∼80 pM. Overall, the adrenaline-bound receptor structure is similar to the others, but it has substantial rearrangements in extracellular loop three and the extracellular tip of transmembrane helix 6. These structures also reveal a water-mediated hydrogen bond between two conserved tyrosines, which appears to stabilize the active state of the ß2AR and related GPCRs.


Assuntos
Agonistas de Receptores Adrenérgicos beta 2/farmacologia , Epinefrina/farmacologia , Engenharia de Proteínas , Receptores Adrenérgicos beta 2/química , Receptores Adrenérgicos beta 2/metabolismo , Anticorpos de Cadeia Única/farmacologia , Benzoxazinas/farmacologia , Sítios de Ligação/efeitos dos fármacos , Cristalografia por Raios X , Evolução Molecular Direcionada , Humanos , Ligação de Hidrogênio/efeitos dos fármacos , Isoproterenol/análogos & derivados , Isoproterenol/farmacologia , Ligantes , Modelos Moleculares , Estabilidade Proteica/efeitos dos fármacos , Receptores Adrenérgicos beta 2/efeitos dos fármacos , Anticorpos de Cadeia Única/genética , Tirosina/química , Tirosina/metabolismo , Água/química , Água/farmacologia
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