RESUMO
The seeds of grass pea (Lathyrus sativus L.), a drought tolerant crop, were analysed for quantitative determination of the free amino acids ß-N-oxalyl-l-α,ß-diaminopropionic acid (ß-ODAP), homoarginine and asparagine by a simple and fast capillary electrophoretic method. In boric acid (80mM, pH 8.0) running buffer system, not only were α and ß-ODAP successfully separated, but also an efficient sample stacking was achieved during hydrodynamic sample introduction. The validated method was used for quantification of ß-ODAP, homoarginine and asparagine in seed extracts of 52 Lathyrus local landraces from various regions of Turkey and one released cultivar. The concentration ranges of amino acids were found as 0.21-1.27% (w/w) for homoarginine, 0.10-0.87% (w/w) for ß-ODAP and 0.006-0.47% (w/w) for asparagine. A positive correlation between homoarginine and ß-ODAP quantities in seeds of 53 Lathyrus local landraces was shown to exist (r(2)=0.649).
