1.
J Immunol Methods
; 190(1): 117-25, 1996 Mar 28.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8601704
RESUMO
The competitive binding of a panel of monoclonal antibodies against hemocyanin of Panulirus interruptus hemocyanin was investigated with three different methods. A competitive-binding immunoassay method was more successful in the determination of ternary complexes than gel electrophoresis and gel filtration. The latter two methods could only be applied with antibodies possessing a higher affinity. Eleven monoclonal antibodies were assigned to groups on the basis of their interactions with five antigenic regions.