RESUMO
The immunomorphological reaction of regional lymphoid organs, the pathomorphology of wound tissues, humoral antitoxic response and a protective effect after local (wound) booster immunization with tetanus toxoid have been studied in observations on 100 guinea pigs with experimental wound infection. The study has shown that the local application of tetanus toxoid, besides stimulating humoral response, induces a more rapid effect aimed at the primary elements of the infectious process (the germination of spores, the adhesion, colonization and toxin formation of the causative agent), thus facilitating the localization of the focus of infection, the development of reparative processes in the wound and the arresting of the infection.
Assuntos
Imunização Secundária , Toxoide Tetânico/imunologia , Tétano/imunologia , Ferimentos Penetrantes/imunologia , Animais , Formação de Anticorpos/imunologia , Cobaias , Imunidade Celular/imunologia , Linfonodos/imunologia , Linfonodos/patologia , Tétano/patologia , Tétano/prevenção & controle , Toxoide Tetânico/administração & dosagem , Fatores de Tempo , Ferimentos Penetrantes/complicações , Ferimentos Penetrantes/patologiaRESUMO
Morphological studies carried out in the igated loop of the rabbit small intestine, used as a model, have revealed that the action of the enterotoxins of C. perfringens type A reference strain and C. perfringens type A strains isolated in the USSR on the intestinal mucosa is absolutely identical. This action is manifested by the destruction of epithelial cells on the tops of the villi, inflammatory infiltration and considerable exudation of fluid into the lumen of the intestinal loop. Early lesions of the brush border of enterocytes, accompanied by the loss of alkaline phosphatase activity and the disappearance of the glycocalix, have-been histochemically established. C. perfrinegens antienterotoxic serum has proved to be capable of completely neutralizing the histopathological activity of C. perfringens enterotoxin.
Assuntos
Clostridium perfringens , Enterotoxinas/farmacologia , Fosfatase Alcalina/metabolismo , Animais , Relação Dose-Resposta a Droga , Epitélio/efeitos dos fármacos , Epitélio/enzimologia , Epitélio/patologia , Histocitoquímica , Íleo/efeitos dos fármacos , Íleo/enzimologia , Íleo/patologia , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/enzimologia , Mucosa Intestinal/patologia , Ligadura , RNA/metabolismo , Coelhos , Fatores de TempoRESUMO
There were revealed morphological peculiarities of the action of C1. perfringens toxins, types B, C, D, E and F on the cultures of fibroblasts of chick embryo, amniotic cells and intestinal tissue. The toxin type B was characterized by a marked vocuolization of the cell cytoplasm; the action of the toxin of type C was expressed in the swelling of the nuclei and the lysis of the chromatine substance, the toxin of type E casued kariorhexis, and the toxin of type F--hyperchromatosis of the nuclei. All the cultures proved to be insensitive to the toxin of type D. Peculiarity of the morphological affection of the cells permitted to differentiate toxin of type B in the cultures of the fibroblasts of chick embryo, whereas the toxins of types C, E and F--in the cultures of the amniotic cells under control of the reaction of neutralization with the homologous antitoxic sera.