[Autoantibodies to nerve growth factor and astroglial protein S-100 in Rett's syndrome]. / Autoantitela k faktoru rosta nervov i astrotsitarnomu belku S-100 pri sindrome Retta.

The levels of autoantibodies (AAB) to nerve growth factor (NGF) and to S-100 protein were examined in blood of both mentally normal girls and girls with Rett's syndrome (RS). The elevated titer of anti-NGF AAB, but not of anti-S-100 AAB was determined in the blood of patients with RS. There was relative individual stability of anti-NGF AAB level which didn't depend on the stage of the disease. At the same time, a reverse correlation was found between the level of these antibodies and severity of the disease: the mild form of the disease (with relative preservation of the speech elements and locomotor functions as well as with the later age of the onset of the disease and the later development of the regressive symptoms) is characterized by the higher AAB level. Autoimmune processes role (anti-NGF AAB) in formation of RS clinical features is discussed.

[Lytic activity against yeasts in Bacillus brevis culture broth]. / Drozhzheliticheskaia aktivnost' kul'tural'noi zhidkosti Bacillus brevis.

The cultural broth of Bacillus brevis, strain 5/4, is capable of lysing the cell walls of Rhodotorula rubra, Candida utilis and other yeasts. The lytic action of the cultural broth is ascribed to proteolysis.

Accumulation of pyrophosphate and other energy-rich phosphorous compounds under various conditions of yeast growth.

In the cells of hybrid yeast strain Saccharomyces N.C.Y.C. 644 SU3 (Karlsberg collection), a large amount of pyrophosphate (30-300 micro mol per g of dry weight) accumulates whatever the aeration conditions and the contest of glucose in the medium. The content of pyrophosphate is 10-100 times higher than that of ATP. At the early and mid-exponential growth phases two maxima of pyrophosphate accumulation are observable. The periods of maximal pyrophosphate accumulation in yeast coincide with those of the minimal contest of polymeric acid-soluble polyphosphates and intense budding. In the light of the data obtained, the question is discussed as to the relationship between the metabolism of pyrophosphates and acid-soluble polyphosphates in yeats.

[Extramitochondrial energy dependent synthesis of inorganic pyrophosphate in yeasts]. / Vnemitokhondrial'nyi énergozavisimyi sintez neorganicheskogo pirofosfata u drozhzhei

In yeast Saccharomyces N.C.Y.C. 644SU3 the simplest energy donor, inorganic pyrophosphate, can be formed not only in the processes coupled with the respiratory chain, but also under conditions when mitochondrial synthesis of the compound is inhibited (glucose repression, anaerobiosis). Stimulation of inorganic pyrophosphate formed after exogeneous addition of glycolytic system components and inhibition studies suggest that under those conditions the non-mitochondrial energy-dependent formation of inorganic pyrophosphate is due to glycolytic phosphorilation.

[Inorganic pyrophosphate synthesis by the mitochondria of the yeast-like fungus Endomyces magnusii coupled with the work of the respiratory chain]. / Sintez neorganicheskogo pirofosfata mitokhondriiami drozhzhepodobnogo griba Endomyces magnusii, sopriazhennyi s rabotoi dykhatel'noi tsepi

The synthesis of inorganic pyrophosphate by the mitochondria of Endomyces magnusii was found to be coupled with respiration. The mitochondria of E. magnusii can utilize the energy of the phosphoanhydride bond of inorganic pyrophosphate for the synthesis of ATP. The study with inhibitors suggests the participation of inorganic pyrophosphatase and ATPase in this process.