Seasonal dynamics and life histories of three sympatric species of Pseudocalanus in two Svalbard fjords.

Small copepods are the most diverse and numerous group in high-latitude zooplankton, yet our knowledge of important species remains poor because of the difficulties involved in correct species identification. In this study, we use a molecular method of identification, a species-specific polymerase chain reaction, to provide the first description of the seasonal dynamics and life histories of the important genus Pseudocalanus in two Svalbard fjords with contrasting environments. We conducted monthly investigations in the relatively warm and ice-free Adventfjorden, supplemented with seasonal samples from the colder, seasonally ice-covered Billefjorden. We found three species of Pseudocalanus (the Arctic P. acuspes and P. minutus, and the boreal P. moultoni). Pseudocalanus acuspes had a distinct annual life cycle and dominated during summer, when it actively reproduced. Surprisingly, the boreal P. moultoni was present year-round in both fjords and was the dominant species during winter; the presence of all life stages of this species throughout the year suggests a more continuous reproduction. The Arctic P. minutus was the rarest of the three species and was likely able to complete its life cycle in Billefjorden but not in Adventfjorden. Our study demonstrates that closely related species may have different life strategies and environmental preferences, which presumably make high-latitude zooplankton communities more resilient to climate change impacts on genus but not necessarily on species level.

Sea ice decline drives biogeographical shifts of key Calanus species in the central Arctic Ocean.

In recent decades, the central Arctic Ocean has been experiencing dramatic decline in sea ice coverage, thickness and extent, which is expected to have a tremendous impact on all levels of Arctic marine life. Here, we analyze the regional and temporal changes in pan-Arctic distribution and population structure of the key zooplankton species Calanus glacialis and C. hyperboreus in relation to recent changes in ice conditions, based on historical (1993-1998) and recent (2007-2016) zooplankton collections and satellite-based sea ice observations. We found strong correlations between Calanus abundance/population structure and a number of sea ice parameters. These relationships were particularly strong for C. glacialis, with higher numbers being observed at locations with a lower ice concentration, a shorter distance to the ice edge, and more days of open water. Interestingly, early stages of C. hyperboreus followed the same trends, suggesting that these two species substantially overlap in their core distribution area in the Arctic Ocean. Calanus glacialis and C. hyperboreus have been historically classified as shelf versus basin species, yet we conclude that both species can inhabit a wide range of bottom depths and their distribution in the Arctic Ocean is largely shaped by sea ice dynamics. Our data suggest that the core distribution patterns of these key zooplankton are shifting northwards with retreating sea ice and changing climate conditions.

Daily transcriptomes of the copepod Calanus finmarchicus during the summer solstice at high Arctic latitudes.

The zooplankter Calanus finmarchicus is a member of the so-called "Calanus Complex", a group of copepods that constitutes a key element of the Arctic polar marine ecosystem, providing a crucial link between primary production and higher trophic levels. Climate change induces the shift of C. finmarchicus to higher latitudes with currently unknown impacts on its endogenous timing. Here we generated a daily transcriptome of C. finmarchicus at two high Arctic stations, during the more extreme time of Midnight Sun, the summer solstice. While the southern station (74.5 °N) was sea ice-free, the northern one (82.5 °N) was sea ice-covered. The mRNAs of the 42 samples have been sequenced with an average of 126 ± 5 million reads (mean ± SE) per sample, and aligned to the reference transcriptome. We detail the quality assessment of the datasets and the complete annotation procedure, providing the possibility to investigate daily gene expression of this ecologically important species at high Arctic latitudes, and to compare gene expression according to latitude and sea ice-coverage.