Loading and Release of Phenolic Compounds Present in Mexican Oregano (Lippia graveolens) in Different Chitosan Bio-Polymeric Cationic Matrixes.

Mexican oregano (Lippia graveolens) polyphenols have antioxidant and anti-inflammatory potential, but low bioaccessibility. Therefore, in the present work the micro/nano-encapsulation of these compounds in two different matrixes of chitosan (CS) and chitosan-b-poly(PEGMA2000) (CS-b-PPEGMA) is described and assessed. The particle sizes of matrixes of CS (~955 nm) and CS-b-PPEGMA (~190 nm) increased by 10% and 50%, respectively, when the phenolic compounds were encapsulated, yielding loading efficiencies (LE) between 90-99% and 50-60%, correspondingly. The release profiles in simulated fluids revealed a better control of host-guest interactions by using the CS-b-PPEGMA matrix, reaching phenolic compounds release of 80% after 24 h, while single CS retained the guest compounds. The total reducing capacity (TRC) and Trolox equivalent antioxidant capacity (TEAC) of the phenolic compounds (PPHs) are protected and increased (more than five times) when they are encapsulated. Thus, this investigation provides a standard encapsulation strategy and relevant results regarding nutraceuticals stabilization and their improved bioaccessibility.

Cytotoxic Activity of Polyphenol Extracts from Three Oregano Species: Hedeoma patens, Lippia graveolens and Lippia palmeri, and Antiproliferative Potential of Lippia graveolens against Two Types of Breast Cancer Cell Lines (MDA-MB-231 and MCF-7).

Oregano infusions have traditionally been used to treat some diseases related to inflammation and cancer; also, some species have shown antiproliferative activity on cancer cell lines, for example, colon and liver, and this has been attributed to its phytochemical profile, mainly its phenolic compounds. This study aimed to evaluate the cytotoxicity and antiproliferative potential of the polyphenols-rich extracts (PRE) of the oregano species H. patens, L. graveolens, and L. palmeri on breast cancer cell lines. The PRE of the three oregano species were obtained from dried leaves. The extract was characterized by determining antioxidant activity, total phenols content, and identifying the profile of phenolic acids and flavonoids by chromatography UPLC-MS/MS. Furthermore, the cytotoxicity of the extracts was evaluated in vitro on a non-cancer cell line of fibroblast NIH3T3 and the antiproliferative potential on the breast cancer cell lines MDA-MB-231 and MCF-7. L. graveolens showed the highest antioxidant capacity and significantly inhibited the proliferation of MCF-7 and MDA-MB-231 cells at non-cytotoxic concentrations in normal cells, with a similar effect to that cisplatin in MDA-MB-231 cells. Therefore, the polyphenol-rich extract from L. graveolens showed the greatest potential to guide future research on the antiproliferative mechanism of action.

Peptides in Colorectal Cancer: Current State of Knowledge.

Colorectal cancer (CRC) is the second most deadly and the third most commonly diagnosed cancer in the world. CRC treatment is mainly based on surgery, chemotherapy, and even though the probability of complications after surgery is very low, chemo drugs affect the patient's quality of life. Multiple studies have shown a strong correlation between diet and the onset and progression of CRC. Thus, the consumption of dietary nutraceuticals for its treatment and prevention has been suggested as a promising option. Peptides have increasingly become of interest in human health due to their antioxidant, antihypertensive, and anticancer potential. In recent years, there have been extensive reports on peptides with anti-tumor activity, and some studies suggest that peptides modulate cell proliferation, evasion of cell death, and metastasis in malignant cells. Plant-derived peptides such as soybean, bean, and rice have received main attention. In this review, we show evidence of several mechanisms through which bioactive peptides exert anti-tumor activity over in vitro and in vivo CRC models. We also report the current status of major production techniques, as well as limitations and future perspectives. Graphical Abstract.

Pollen morphology of four species of Jatropha (Euphorbiaceae), including toxic varieties, in Northwestern Mexico / Morfología del polen de cuatro especies de Jatropha (Euphorbiaceae), incluyendo variedades tóxicas, en el noroeste de México

AbstractJatropha curcas is a native Mexican plant, distributed in the forests of coastal regions; it has shown potential for the production of biofuel, and its raw protein can be used as animal feed. Nevertheless, its current varieties have low yield and production variability, as it is in domestication period. The knowledge of the pollen grains is fundamental for studies of Jatropha reproductive biology and breeding for genetic improvement to generate new hybrids and improve fruit and seed yield. This study compared pollen morphology of four Jatropha species, which include two varieties of J. curcas (one toxic from India and one non-toxic from Mexico), and two varieties of wild J. cinerea (Sinaloa and Baja California Sur); additionally, pollen was collected from wild J. platyphylla (Sinaloa) and J. vernicosa (Baja California Sur) to characterize them palynologically. We used a scanning electron microscope to describe the size and shape of pollen grains. Pollen grains were observed spheroidal. The diameter of the varieties of toxic and non-toxic J. curcas and that of J. platyphylla were 58 ± 2.3, 54 ± 1.7, and 51 ± 2.4 µm, respectively; the number of clavae was 84 ± 10, 108 ± 15, and 180 ± 15.5, respectively. Pollen grains of J.cinerea (Sinaloa) had a diameter of 50 ± 1.7 µm and 220 ± 14 clavae by area; J. cinerea (Baja California Sur) had a diameter of 45 ± 1.9 µm and 195 ± 14.7 clavae. Pollen grains of J. vernicosa had a diameter of 46 ± 2.1 µm and 231 ± 25.6 clavae. The ornamentation of the exine showed clava with capita gemma shaped for varieties of J.curcas species and clavum shaped for J. platyphylla, J. cinerea and J. vernicosa species. The studied varieties have a cross-linked endexine and ectexine intectate. Luminal bacules were observed in the reticular space of J. curcas varieties while none were present in the varieties of J. cinerea, J. platyphylla, and J. vernicosa.

ResumenJatropha curcas es nativa de México, y se distribuye en los bosques de las regiones costeras; las plantas han mostrado potencial para la producción de biodiesel, y la proteína cruda puede utilizarse para alimentación animal. Sin embargo, sus variedades actuales tienen bajo rendimiento y variabilidad en la producción, por estar en periodo de domesticación. El conocimiento de los granos de polen es fundamental para el estudio de la biología reproductiva de Jatropha y mejoramiento genético para generar nuevos híbridos y mejorar el rendimiento de frutos y semillas. Este estudio compara la morfología del polen de cuatro especies de Jatropha, que incluyen dos variedades de J. curcas, (una tóxica de India y una no tóxica de México) y dos variedades silvestres de J. cinerea (Sinaloa y Baja California Sur), por último, se obtuvo polen de J. platyphylla (Sinaloa) y J. vernicosa (Baja California Sur) silvestres para caracterizarlos palinológicamente. Para describir el tamaño y la forma de los granos de polen, se utilizó un microscopio electrónico de barrido. Los granos de polen tienen forma esferoidal. El diámetro de las variedades de J. curcas tóxica y no tóxica, y J. platyphylla fueron 58 ± 2.3, 54 ± 1.7, y 51 ± 2.4 micras, respectivamente; el número de clavas fue de 84 ± 10, 108 ± 15 y 180 ± 15.5, respectivamente. Los granos de polen de J. cinerea (Sinaloa) tienen un diámetro de 50 ± 1.7 micras y 220 ± 14 clavas por zona; J. cinerea (Baja California Sur) tiene un diámetro de 45 ± 1.9 micras y 195 ± 14.7 clavas. Los granos de polen de J. vernicosa tienen un diámetro de 46 ± 2.1 micras y 231 ± 25.6 clavas. La ornamentación de la exina mostró clavas con cápita en forma de gema y pequeños báculos en el lumen para ambas J. curcas y en forma de clavum para J. cinerea, J. platyphylla y J. vernicosa y no presentan báculos en el lumen. Las variedades estudiadas tienen una endexina reticulada y ectexina intectada.

Protective Role of Flavonoids and Lipophilic Compounds from Jatropha platyphylla on the Suppression of Lipopolysaccharide (LPS)-Induced Inflammation in Macrophage Cells.

Seventeen polyphenols (e.g, apigenin, genistein, and luteolin glycosides) and 11 lipophilic compounds (e.g., fatty acids, sterols, and terpenes) were detected by LC-MS/MS-ESI and GC-MS, respectively, in Jatropha platyphylla. Extracts from pulp, kernel, and leaves and fractions were studied to know their effect on some pro-inflammatory mediators. Phenolic and lipophilic extracts showed significant inhibitory effects on ROS and NO production while not affecting mitochondrial activity or superoxide generation rate in lipopolysaccharide (LPS)-induced inflammation in RAW 264.7 macrophage cells. In addition, NO production was also diminished by lipophilic leaf fractions F1 and F2 with the latter fraction showing a greater effect and composed mainly of sterols and terpene. Furthermore, total extracts showed nonselective inhibitions against cyclooxygenase COX-1 and COX-2 activities. All together, these results suggest that J. platyphylla extracts have potential in treating inflammatory diseases and their activity is mediated by flavonoids and lipophilic compounds.

Diagnóstico evaluativo para el fortalecimiento de estrategias de intervención en salud mental en Argentina / Diagnostic evaluation for the strengthening of strategies of intervention in mental health in Argentina

El estudio muestra los principales hallazgos de un diagnóstico evaluativo sobre políticas, planes y servicios que brindan cuidados en salud mental en Argentina. Con un diseño plural cuali-cuantitativo y un carácter multicéntrico y colaborativo, su desarrollo incorporó diversas contribuciones teórico-metodológicas, a partir del relevamiento de información de diferentes fuentes primarias y secundarias. Se trabajó sobre seis áreas geográficas que ya contaban con legislación para salud mental en 2007: Río Negro, Córdoba, EntreRíos, San Juan, Santa Fe y Ciudad Autónoma de Buenos Aires. Su objetivo fue producir conocimientos que contribuyeran a estimular el debate y a priorizar la salud mental en la agenda de las políticas sanitarias. Tomando por referencia los principios que impulsan los procesos internacionales de reforma de la atención en salud mental, se señala que Argentina aún carece de una ley nacional de salud mental. La expansión de los servicios de salud mental articulados a la atención primaria muestra una dirección ya consistente y asumida en las políticas sanitarias. A excepción de Río Negro, en las demás jurisdicciones se evidencia un modelo dual que superpone la atención hospitalaria centrada en la enfermedad mental junto a incipientes estrategias y dispositivos alternativos insertos en la vida comunitaria. La escasa información epidemiológica compone un cuadro disperso que no permite conocer problemáticas prevalentes ni priorizar grupos poblacionales vulnerables

The study shows the main findings from a diagnostic evaluation up on strategies, planning and services formental health care in Argentina. With an extensive qualitative and quantitative design and a multicentric and collaborative character, the study has incorporated several theoretical and methodological contributions and information primary and secondary sources. It has worked in five areas that had legislation for mental health in 2007: Río Negro, Córdoba, Entre Ríos, San Juan, Santa Feand Ciudad Autónoma de Buenos Aires. It intended to produce know ledge to stimulate the debate and give a priority status to mental health in the agenda of health policies. Taking into account, as a reference, the principles that gave impulse to the international processes to reform the attention in mental health, it is important to point out that Argentina do not have, yet, a national law for mental health. The expansion of services in mental health, articulated to primary care, shows a consistent direction assumed by the sanitary policies. Exception made to Río Negro, in the remaining jurisdictions there is a dual model that overlaps the hospital centered care in mental health, together with incipient strategies and alternative devices, inserted in the communitarian life. The very little epidemiological information created an elusive panorama that makes impossible to reach the core of the main problems, or give a priority attendance to the most vulnerable population