RESUMO
Recently, a contamination incident was described in which the challenge inoculum used in a bluetongue virus serotype 8 (BTV-8) vaccination trial was contaminated with a BTV-11 virus that was closely related to the Belgian BTV-11 virus from 2008. This study reports the first complete genome sequences of four BTV-11 viruses: the BTV-11 contaminant, BTV-11 reference strain, BTV-11 vaccine strain and a recently isolated BTV-11 field strain from Martinique. Full-genome analysis showed that these viruses belong to serotype 11/nucleotype A and cluster together with other western topotype bluetongue viruses. Detailed comparisons of the genomes further indicated that the contaminant was derived from the BTV-11 reference strain, as they were distinguished by a single synonymous nucleotide substitution. The previously reported partial sequence of genome segment 2 of the Belgian BTV-11 was found to be identical to that of the BTV-11 vaccine strain, indicating that it most likely was the BTV-11 vaccine strain. These findings also suggest that the BTV-11 contaminant and the Belgian BTV-11 are not the same viruses. Finally, comparison of the reference and vaccine strain did not allow determining the amino acid substitutions that contribute to the attenuated phenotype.
Assuntos
Vírus Bluetongue/genética , Bluetongue/prevenção & controle , Genoma Viral/genética , Vacinas Virais , Animais , Sequência de Bases , Vírus Bluetongue/classificação , Vírus Bluetongue/imunologia , Vírus Bluetongue/isolamento & purificação , Europa (Continente) , Dados de Sequência Molecular , Filogenia , Sorogrupo , Ovinos , Vacinação/veterinária , Vacinas Virais/administração & dosagemRESUMO
Between August 2010 and February 2011, over 750 deaths were recorded among wild goats (Capra aegagrus, the endangered progenitor of the domestic goat) in Kurdistan. Based on the clinical signs and post-mortem findings, the involvement of peste des petits ruminants virus (PPRV) was suspected. This was confirmed by laboratory analysis, and the virus was found to be closely related to a Turkish strain isolated in 2000. During the outbreak in wild goats, no disease in domestic animals was reported. Domestic ruminants in the region are routinely vaccinated with an attenuated vaccine based on the 'Nigeria/75/1' strain of PPRV. This is the first report of active PPRV infection in Kurdistan and most likely the immunity afforded by vaccination prevented spillover infections. It is therefore recommended to continue with the campaign. Conversely, there is no justification for the use of force to keep the endangered wild goats away from domestic flocks.
