Antimicrobial and cytotoxic properties of calcium-enriched mixture cement, Iranian propolis, and propolis with herbal extracts in primary dental pulp stem cells.

Objectives: In this study, natural substances were introduced as primary dental pulp caps for use in pulp therapy, and the antimicrobial and cytotoxic properties of these substances were investigated. Materials and Methods: In this in vitro study, the antimicrobial properties of calcium-enriched mixture (CEM) cement, propolis, and propolis individually combined with the extracts of several medicinal plants were investigated against Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. Then, the cytotoxicity of each substance or mixture against pulp stem cells extracted from 30 primary healthy teeth was evaluated at 4 concentrations. Data were gathered via observation, and optical density values were obtained using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test and recorded. SPSS software version 23 was used to analyze the data. Data were evaluated using 2-way analysis of variance and the Tukey test. Results: Regarding antimicrobial properties, thyme alone and thyme + propolis had the lowest minimum inhibitory concentrations (MICs) against the growth of S. aureus, E. coli, and P. aeruginosa bacteria. For E. faecalis, thyme + propolis had the lowest MIC, followed by thyme alone. At 24 and 72 hours, thyme + propolis, CEM cement, and propolis had the greatest bioviability in the primary dental pulp stem cells, and lavender + propolis had the lowest bioviability. Conclusions: Of the studied materials, thyme + propolis showed the best results in the measures of practical performance as a dental pulp cap.

The effects of Pre-hospital Trauma Life Support (PHTLS) training program on the on-scene time interval.

BACKGROUND: Recent studies have shown that reducing pre-hospital time could improve the outcomes of trauma victims. Due to the importance of pre-hospital time management, this study aims to determine the effects of the Pre-hospital Trauma Life Support (PHTLS) training program on the on-scene time interval reduction. METHODS: The PHTLS training program was implemented based on global standards for pre-hospital emergency technicians. The research tool was a questionnaire designed by the Ministry of Health and Medical Education in Iran. The mean on-scene time interval was calculated before, after and one month after the intervention in the control (n = 32) and experimental group (n = 32). The data were analyzed using SPSS. RESULTS: The mean on-scene time interval in the target group (one month after intervention) has been significantly lower than that of the control group. Moreover, the mean and standard deviation from the on-scene time interval in the target group has been reduced from 17.6 ± 5.5 (before intervention) to 12 ± 3.8 min (one month after intervention) which was statistically significant. CONCLUSION: The implementation of the PHTLS training program can lead to the reduction of on-scene time interval. Therefore, considering the role of reducing on-scene time intervals on victims' survival, the integration of the PHTLS training programs with pre-hospital emergency medical service systems seems inevitable.

Evaluation of the disintegration of linen fabric under composting conditions.

In recent years, about 10% of the total wastes generated around the world were fabric wastes and, due to the large volume of these wastes, should be well managed. Among the various types of fabrics, some have greater biodegradability and can be decomposed at the acceptable rates under controlled conditions. In this study, the disintegration of linen fabric was investigated under composting conditions. During the composting process, temperature, moisture, and aeration were controlled. According to FTIR spectra of linen fabrics during the composting process, the disintegration of linen fibers was identified as the breakdown of carbonyl groups and hydrogen bonds of cellulosic chains. Regarding the SEM images of linen fabrics, the composting process in the first 45 days led to the breakdown of the fibers, and in the second 45 days of the process, most of the fibers were degraded. Since in the second 45 days of the process, the compost pile temperature is always between 39 and 44 °C and 65-70 °C, it can be concluded that the rate of microbial disintegration of the linen fabric is higher at these temperatures. At the end of the test, the disintegration value of the linen fabric was 55% and the weight loss due to organic matter conversion was 61%. The samples weight loss showed that linen fabric has high biodegradability under composting conditions. The germination index (GI) of the resulting compost was 73.88%, which indicates the produced compost is mature.

Deep phenotyping: deep learning for temporal phenotype/genotype classification.

BACKGROUND: High resolution and high throughput genotype to phenotype studies in plants are underway to accelerate breeding of climate ready crops. In the recent years, deep learning techniques and in particular Convolutional Neural Networks (CNNs), Recurrent Neural Networks and Long-Short Term Memories (LSTMs), have shown great success in visual data recognition, classification, and sequence learning tasks. More recently, CNNs have been used for plant classification and phenotyping, using individual static images of the plants. On the other hand, dynamic behavior of the plants as well as their growth has been an important phenotype for plant biologists, and this motivated us to study the potential of LSTMs in encoding these temporal information for the accession classification task, which is useful in automation of plant production and care. METHODS: In this paper, we propose a CNN-LSTM framework for plant classification of various genotypes. Here, we exploit the power of deep CNNs for automatic joint feature and classifier learning, compared to using hand-crafted features. In addition, we leverage the potential of LSTMs to study the growth of the plants and their dynamic behaviors as important discriminative phenotypes for accession classification. Moreover, we collected a dataset of time-series image sequences of four accessions of Arabidopsis, captured in similar imaging conditions, which could be used as a standard benchmark by researchers in the field. We made this dataset publicly available. CONCLUSION: The results provide evidence of the benefits of our accession classification approach over using traditional hand-crafted image analysis features and other accession classification frameworks. We also demonstrate that utilizing temporal information using LSTMs can further improve the performance of the system. The proposed framework can be used in other applications such as in plant classification given the environment conditions or in distinguishing diseased plants from healthy ones.

Antibacterial Efficacy of Calcium Hydroxide and Chlorhexidine Mixture for Treatment of Teeth with Primary Endodontic Lesions: A Randomized Clinical Trial.

INTRODUCTION: This study compared the root canal microbial count of necrotic teeth after irrigation with 6% sodium hypochlorite (NaOCl) (single session treatment) and two-session root canal treatment with two-week application of calcium hydroxide (CH) mixed with 0.2% chlorhexidine (CHX) as intracanal medicament. METHODS AND MATERIALS: In this randomized clinical trial, single-rooted necrotic teeth were divided into two groups. Root canal was irrigated with 2 mL of 6% NaOCl in one group, and a mixture of 0.2% CHX and CH powder as an intracanal medicament for two weeks, in the other group. Root canal samples were obtained before and after the intervention and number of colony forming units (CFUs) was counted in each phase. RESULTS: The reduction of Enterococcus faecalis CFU was not significantly different between the two groups (P=0.233) but the CFU of aerobic and anaerobic bacteria was significantly lower in CH+CHX group (P<0.001). CONCLUSION: Two-week application of CH+CHX caused significant reduced the aerobic, anaerobic and E. faecalis colony counts. Thus, it may be beneficial to carry out primary root canal treatment of necrotic teeth with endodontic lesions in two sessions with intracanal medicaments to achieve predictable results.

Cone-Beam Computed Tomography Study Of Crown Dilaceration With a Talon Cusp in an Unerupted Permanent Maxillary Tooth.

This article describes a rare case of crown dilaceration with a talon cusp in an unerupted permanent maxillary central incisor. Our patient was a 7-year-old boy with a history of trauma to his primary maxillary teeth (#51 and 52), at 3 years of age complaining of failure of eruption of tooth #11. Periapical radiography showed incomplete formation of tooth root #11 and more superior position of tooth bud #11 relative to tooth bud #12. A cone-beam computed tomography was ordered, which revealed crown dilaceration with a talon cusp in tooth bud #11. The patient was scheduled for follow-up at 6 months.

The Status of School Events in Middle School Students in Isfahan Province.

BACKGROUND: Adolescents are being threatened by several accidents because of entering the new world of adulthood, separation from families, a greater presence in community individually, the lack of knowledge of rules and risk-taking spirit of adolescents, and events. The contribution of these damages and their related costs are higher in developing and low-income countries. The current study was conducted for proper planning focused on reducing accidents that have the highest incidence, evaluating school events, determining the place and time that most accidents happened to students and determining the body part injured. METHODS: A cross-sectional study using a multistage cluster sampling was conducted on 6933 students 11-14 years old (middle school) in 2009-2010 in Isfahan Province. The data were collected through observation and a researcher-made questionnaire and then entered into the EPi6 software, and analyzed by the SPSS 11.5 software and the statistical test of Chi-square. RESULTS: The findings showed that 49.9% of students were girls (n = 3462) and 50.1% (n = 3471) were boys, 84.5% of students (n = 5860) were living in urban areas and 15.5% of students (n = 1073) were living in rural areas. Frequency distribution of incidents was 53.9% (3739 cases) at school and 10.6% (732 cases) on the commuting route. The most injured body part was hand with 1018 cases (18.5%) and foot with 1267 cases (23.1%), and mostly they were injured in boys. CONCLUSIONS: The findings showed that a high percentage of students were injured at school, where the first step to prevent the incidence of such preventable incidents at school is to secure workshop environments and school yards.