RESUMO
Canine filariosis is caused by filiform nematodes and affects several species of animals as well as humans. The disease produces a wide range of symptoms that can often be confused with other diseases, which increases the complexity of its diagnosis. The search for methodologies to facilitate its diagnosis is a challenge, and specific and differential identification of the parasite species causing the disease holds key to a successful diagnosis. In Colombia, there is a problem of underdiagnosis of filariosis in microfilaremic dogs infected by Dirofilaria immitis and Acanthocheilonema reconditum, and of microfilaremias not related to heartworm disease. The highest prevalences have been reported for D. immitis infections, although new cases of A. reconditum infections are beginning to appear. The aim of this study was to differentiate the microfilariae infections caused by D. immitis and A. reconditum by a morphological and molecular characterization of microfilariae so as to facilitate an accurate diagnosis of canine filariosis in the metropolitan area of Bucaramanga (Colombia). For this purpose, 400 blood samples with anticoagulants were collected from the dogs and analyzed with the help of a commercial immunochromatography kit for the detection of D. immitis circulating antigen. The Woo, Knott, and polymerase chain reaction (PCR) techniques were employed for determining the parasite count, morphological observation, and molecular identification of microfilariae present in the dogs respectively. The prevalence of microfilaremic dogs in Bucaramanga metropolitan area was 18.75% (75/400). The prevalence of dogs that tested positive for D. immitis in the antigen and in PCR tests was 1.25% (5/400) and 1% (4/400), respectively. Furthermore, the PCR test revealed that 17.75% of the microfilaremic dogs tested positive for A. reconditum (71/400) (first report in the metropolitan area of Bucaramanga), with one animal co-infected by both species, and 0% for D. repens (0/400). However, by morphological characterization, 4% of the microfilariae (3/75) corresponded to D. immitis, 20% (15/75) to D. repens, and 76% (57/75) to A. reconditum. The use of molecular diagnostic methods such as PCR aids in the specific identification of the parasite, thus making it a more accurate method than the morphological characterization of microfilariae. The identification of the parasites by PCR helps improve the veterinary diagnosis of canine filariosis in Colombia, which would lead to the establishment of an appropriate treatment protocol for each species of filaria and also to the generation of reliable data to be used at the clinical and epidemiological levels.
RESUMO
The cardiopulmonary dirofilariosis caused by Dirofilaria immitis, is a vector-borne infection, which can be transmitted to humans. The main hosts are both domestic and wild canids. This species mainly occurs in tropical and subtropical climates, and temperature and humidity are the main factors that favor the presence and proliferation of culicid mosquitoes as vectors of the disease. There are few reports of cardiopulmonary dirofilariosis in dogs and humans in Colombia, a region with favorable climatic conditions which favors the presence of mosquitoes that act as vectors of the disease. Therefore, this study aimed to examine its current prevalence in dogs and the risk of human exposure to the disease in Bucaramanga, one of the most populated areas in Colombia located at the center of the country. Furthermore, its demographic and environmental characteristics could be useful as a study model for other similar locations and neighboring countries. Serum samples from 351 dogs and 506 humans from the Bucaramanga Metropolitan area were analyzed. All dog samples were analyzed by Knott's technique and tested with a commercial immunochromatographic to detect the presence of circulating antigens of D. immitis. Human samples were analyzed using a non-commercial ELISA test kit to detect IgG against the somatic antigens of adult D. immitis and Wolbachia. Positive results were further confirmed using western blot analysis. Thirty-eight dogs tested positive with a overall prevalence of 10.82%. Of these dogs, 18 showed D. immitis microfilariae, being 5.12% of the total population. The overall seroprevalence in humans was 6.71%; seroprevalence was significantly higher in individuals aged 16-34 years-old and in women than in men. To our knowledge, this study describes seropositivity to D. immitis for the first time in a Colombian human population located in the same area as that of dogs infected with D. immitis, which represents a potential threat to public health. In humans, age and gender can be considered risk factors for exposure to D. immitis.
RESUMO
In the last few years, an unusual increase in the number of acute Chagas disease outbreaks, presumably due to oral transmission, has been reported in urban areas in Santander, Colombia. Given the importance of dogs (Canis lupus familiaris) as reservoir hosts and sentinels of T. cruzi infection across different regions of America, we carried out a serological and molecular survey on T. cruzi infection in 215 dogs from the metropolitan area of Bucaramanga, Santander. Serological detection was carried out using enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), and indirect hemagglutination assay (IHA), while molecular detection was done using a nested PCR (nPCR), targeting the microsatellite region of T. cruzi nuclear DNA. Animals were defined as seropositive when at least two of the three serological tests were positive, and only these animals were evaluated with the nPCR. To discriminate DTU TcI from other DTUs, a multiplex PCR was performed in the T. cruzi-positive samples. Additionally, clinical and hematological traits were evaluated in these hosts. The dog sera showed a seropositivity rate of 27.9 % (60/215), of which 43.3 % (26/60) were positive for nPCR. Statistical analysis indicated that T. cruzi seropositive in dogs was associated with specific socioeconomic sectors and a lack of garbage collection in these municipalities. Hematological analyses showed that T. cruzi infection was associated with anemia and platelet alterations but not with alterations of aspartate aminotransferase (ASAT) and creatine kinase myocardial band (CK-MB). The high seroprevalence of infection and active circulation of T. cruzi I (TcI) in dogs reflect the risk of infection to humans in this area, which should be taken into consideration when Chagas disease control programs are implemented. In addition, T. cruzi infection may take a toll on dog health, which should be considered during dog care and management.
