RESUMO
Background. Orthodontic tooth movement (OTM) occurs in the alveolar bone; therefore, any condition affecting bone quality can alter OTM. This study aimed to evaluate the effect of amitriptyline on OTM in rats. Methods. Forty-five male Wistar rats were randomly divided into three groups: (I) no injection, (II) injection with saline solution, and (III) injection of amitriptyline. Next, a 60-gr force was applied to the maxillary left first molar tooth of all the rats, using a nickelâtitanium closed-coil spring ligated between the maxillary incisors and the left first molar tooth. The rats were sacrificed after 21 days to measure OTM and perform histological analysis to determine the number, width, and depth of resorptive lacunae, osteoclast counts, and periodontal ligament (PDL) width. Results. The highest and the lowest OTM rates were found in the control and amitriptyline groups, respectively; however, there was no significant difference between the study groups in this regard. Histological analysis showed a significantly lower number of resorption lacunae in the amitriptyline group than the saline group. Conclusion. Although no significant difference was noted in OTM after amitriptyline administration, a reduction in the number of resorptive lacunae in rats injected with amitriptyline suggests that amitriptyline affects the bone tissue at the cellular level.
RESUMO
CONTEXT: Fascin is an actin-bundling protein that has been shown to be overexpressed in various carcinomas, but the effect of doxorubicin on its protein and mRNA has not been investigated. AIMS: The aim of the present study is to evaluate any changes that may be induced by doxorubicin on the fascin molecule in a cancer cell line. SETTINGS AND DESIGN: Experimental study. MATERIALS AND METHODS: The expression of fascin protein and mRNA in the human KB carcinoma cell line was evaluated by qRT-PCR, and immunocytochemistry following treatment with doxorubicin. Apoptosis was also analyzed using flow cytometry. STATISTICAL ANALYSIS USED: Group comparisons were performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test to determine differences between groups (P < 0.05). RESULTS: Treatment with doxorubicin promoted a significant increase of fascin mRNA and decrease of fascin protein (P <0.05). Doxorubicin also induced apoptosis in the KB cell line. CONCLUSIONS: Regarding the changes in the actin-bundling protein, fascin, which was found following treatment with doxorubicin, it can be hypothesized that this drug may possess antimigratory characteristics by influencing fascin. Further studies are suggested to clarify this theory.
