Stranded cetaceans warn of high perfluoroalkyl substance pollution in the western Mediterranean Sea.

Perfluoroalkyl substances (PFASs) are a class of organohalogenated compounds of environmental concern due to similar characteristics as the well-studied legacy persistent organic pollutants (POPs) that typically show environmental persistence, biomagnification and toxicity. Nevertheless, PFAS are still poorly regulated internationally and in many aspects poorly understood. Here, we studied liver and muscle concentrations in five cetacean species stranded at the southeastern coast of Spain during 2009-2018. Twelve of the fifteen targeted compounds were detected in >50% of the liver samples. Hepatic concentrations were significantly higher than those in muscle reflecting the particular toxicokinetics of these compounds. Bottlenose dolphins Tursiops truncatus showed the highest hepatic ΣPFAS (n = 5; 796.8 ± 709.0 ng g-1 ww) concentrations, followed by striped dolphin Stenella coeruleoalba (n = 29; 259.5 ± 136.2 ng g-1 ww), sperm whale Physeter macrocephalus (n = 1; 252.8 ng g-1 ww), short-beaked common dolphin Delphinus delphis (n = 2; 240.3 ± 218.6 ng g-1 ww) and Risso's dolphin Grampus griseus (n = 1; 78.7 ng g-1 ww). These interspecies differences could be partially explained by habitat preferences, although they could generally not be related to trophic position or food chain proxied by stable N (δ15N) and C (δ13C) isotope values, respectively. PFAS profiles in all species showed a similar pattern of concentration prevalence in the order PFOS>PFOSA>PFNA≈PFFUnA>PFDA. The higher number of samples available for striped dolphin allowed for evaluating their PFAS burden and profile in relation to the stranding year, stable isotope values, and biological variables including sex and length. However, we could only find links between δ15N and PFAS burdens in muscle tissue, and between stranding year and PFAS profile composition. Despite reductions in the manufacturing industry, these compounds still appear in high concentrations compared to more than two decades ago in the Mediterranean Sea and PFOS remains the dominating compound.

White-tailed eagle (Haliaeetus albicilla) and great cormorant (Phalacrocorax carbo) nestlings as spatial sentinels of Baltic acidic sulphate soil associated metal contamination.

Sulphate soils, characterised by low pH conditions, are found worldwide, and are potentially large sources of metal contamination, often exceeding industrial emissions. Metal leaching from sulphate soils has been shown to be harmful to aquatic organisms, but the cascading effect on exposure in apex avian predators has not been studied earlier. With the present study we aimed at evaluating the potential of white-tailed eagle (Haliaeetus albicilla) and great cormorant (Phalacrocorax carbo) nestlings, collected from nests located either in sulphate soil or control areas, for monitoring spatial contaminant trends of metals typically associated with sulphate soils. In blood of white-tailed eagles, the concentrations of aluminium and cobalt were significantly higher in sulphate soil areas. In blood of great cormorants, the concentrations of copper and manganese were so, while the concentration of zinc was found to be lower. Also, we observed an interaction between the latitude and soil type in cobalt and lithium concentrations of great cormorants, showing that concentrations in the sulphate soil associated nestlings rose more steeply towards the north than in the control group. Latitudinal trends of higher concentrations in the south were found in cadmium, manganese, and copper of white-tailed eagle nestlings, while thallium of white-tailed eagle nestlings, and thallium and zinc of great cormorant nestlings showed a latitudinal trend of higher concentrations in the north. Concentrations of several metals correlated positively within a species indicating covariation in metal exposure. Generally, the metal concentrations in both species were similar to levels reported to be below toxicity thresholds in other species. These results indicate, that white-tailed eagle and great cormorant nestling metal burdens may indicate environmental contamination from acidic sulphate soil runoff, and that they may act as indicators of latitudinal gradient identifying different contamination sources.

Per- and polyfluoroalkyl substances in plasma and feathers of nestling birds of prey from northern Norway.

Plasma samples from nestlings of two top predators, White-tailed eagle (Haliaeetus albicilla) and Northern goshawk (Accipiter gentilis) from northern Norway were analysed for a wide range of per- and polyfluoroalkyl substances (PFASs). Body feathers from the White-tailed eagles were also analysed and significant associations between specific PFASs in blood plasma and body feathers were found (0.36

Tracking pan-continental trends in environmental contamination using sentinel raptors-what types of samples should we use?

Biomonitoring using birds of prey as sentinel species has been mooted as a way to evaluate the success of European Union directives that are designed to protect people and the environment across Europe from industrial contaminants and pesticides. No such pan-European evaluation currently exists. Coordination of such large scale monitoring would require harmonisation across multiple countries of the types of samples collected and analysed-matrices vary in the ease with which they can be collected and the information they provide. We report the first ever pan-European assessment of which raptor samples are collected across Europe and review their suitability for biomonitoring. Currently, some 182 monitoring programmes across 33 European countries collect a variety of raptor samples, and we discuss the relative merits of each for monitoring current priority and emerging compounds. Of the matrices collected, blood and liver are used most extensively for quantifying trends in recent and longer-term contaminant exposure, respectively. These matrices are potentially the most effective for pan-European biomonitoring but are not so widely and frequently collected as others. We found that failed eggs and feathers are the most widely collected samples. Because of this ubiquity, they may provide the best opportunities for widescale biomonitoring, although neither is suitable for all compounds. We advocate piloting pan-European monitoring of selected priority compounds using these matrices and developing read-across approaches to accommodate any effects that trophic pathway and species differences in accumulation may have on our ability to track environmental trends in contaminants.

Measuring environmental stress in East Greenland polar bears, 1892-1927 and 1988-2009: what does hair cortisol tell us?

Hair sampled from 96 East Greenland polar bears (Ursus maritimus) over the periods 1892-1927 and 1988-2009 was analyzed for cortisol as a proxy to investigate temporal patterns of environmental stress. Cortisol concentration was independent of sex and age, and was found at significantly higher (p<0.001) concentrations in historical hair samples (1892-1927; n=8) relative to recent ones (1988-2009; n=88). In addition, there was a linear time trend in cortisol concentration of the recent samples (p<0.01), with an annual decrease of 2.7%. The recent hair samples were also analyzed for major bioaccumulative, persistent organic pollutants (POPs). There were no obvious POP related time trends or correlations between hair cortisol and hair POP concentrations. Thus, polar bear hair appears to be a relatively poor indicator of the animal's general POP load in adipose tissue. However, further investigations are warranted to explore the reasons for the temporal decrease found in the bears' hair cortisol levels.