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1.
Genetics ; 225(2)2023 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-37232380

RESUMO

The maize female gametophyte contains four cell types: two synergids, an egg cell, a central cell, and a variable number of antipodal cells. In maize, these cells are produced after three rounds of free-nuclear divisions followed by cellularization, differentiation, and proliferation of the antipodal cells. Cellularization of the eight-nucleate syncytium produces seven cells with two polar nuclei in the central cell. Nuclear localization is tightly controlled in the embryo sac. This leads to precise allocation of the nuclei into the cells upon cellularization. Nuclear positioning within the syncytium is highly correlated with their identity after cellularization. Two mutants are described with extra polar nuclei, abnormal antipodal cell morphology, and reduced antipodal cell number, as well as frequent loss of antipodal cell marker expression. Mutations in one of these genes, indeterminate gametophyte2 encoding a MICROTUBULE ASSOCIATED PROTEIN65-3 homolog, shows a requirement for MAP65-3 in cellularization of the syncytial embryo sac as well as for normal seed development. The timing of the effects of ig2 suggests that the identity of the nuclei in the syncytial female gametophyte can be changed very late before cellularization.


Assuntos
Óvulo Vegetal , Zea mays , Zea mays/genética , Diferenciação Celular , Óvulo Vegetal/genética , Sementes/genética
2.
Plant Reprod ; 33(3-4): 117-128, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-32865620

RESUMO

Gametophytic cross-incompatibility systems in corn have been the subject of genetic studies for more than a century. They have tremendous economic potential as a genetic mechanism for controlling fertilization without controlling pollination. Three major genetically distinct and functionally equivalent cross-incompatibility systems exist in Zea mays: Ga1, Tcb1, and Ga2. All three confer reproductive isolation between maize or teosinte varieties with different haplotypes at any one locus. These loci confer genetically separable functions to the silk and pollen: a female function that allows the silk to block fertilization by non-self-type pollen and a male function that overcomes the block of the female function from the same locus. Identification of some of these genes has shed light on the reproductive isolation they confer. The identification of both male and female factors as pectin methylesterases reveals the importance of pectin methylesterase activity in controlling the decision between pollen acceptance versus rejection, possibly by regulating the degree of methylesterification of the pollen tube cell wall. The appropriate level and spatial distribution of pectin methylesterification is critical for pollen tube growth and is affected by both pectin methylesterases and pectin methylesterase inhibitors. We present a molecular model that explains how cross-incompatibility systems may function that can be tested in Zea and uncharacterized cross-incompatibility systems. Molecular characterization of these loci in conjunction with further refinement of the underlying molecular and cellular mechanisms will allow researchers to bring new and powerful tools to bear on understanding reproductive isolation in Zea mays and related species.


Assuntos
Genes de Plantas , Zea mays , Cruzamento , Genes de Plantas/genética , Pólen/genética , Tubo Polínico , Polinização , Reprodução/genética , Autoincompatibilidade em Angiospermas/genética , Zea mays/genética
3.
Curr Top Dev Biol ; 140: 257-282, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32591076

RESUMO

The plant haploid generation is specified late in higher plant development, and post-meiotic haploid plant cells divide mitotically to produce a haploid gametophyte, in which a subset of cells differentiates into the gametes. The immediate mother of the angiosperm seed is the female gametophyte, also called the embryo sac. In most flowering plants the embryo sac is comprised of two kinds of gametes (egg and central cell) and two kinds of subsidiary cells (antipodals and synergids) all of which descend from a single haploid spore produced by meiosis. The embryo sac develops within a specialized organ of the flower called the ovule, which supports and controls many steps in the development of both the embryo sac and the seed. Double fertilization of the central cell and egg cell by the two sperm cells of a pollen grain produce the endosperm and embryo of the seed, respectively. The endosperm and embryo develop under the influence of their precursor gametes and the surrounding tissues of the ovule and the gametophyte. The final size and pattern of the angiosperm seed then is the result of complex interactions across multiple tissues of three different generations (maternal sporophyte, maternal gametophyte, and the fertilization products) and three different ploidies (haploid gametophyte, diploid parental sporophyte and embryo, and triploid endosperm).


Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Magnoliopsida/genética , Meiose/genética , Óvulo Vegetal/genética , Sementes/genética , Arabidopsis/genética , Arabidopsis/crescimento & desenvolvimento , Endosperma/genética , Endosperma/crescimento & desenvolvimento , Magnoliopsida/classificação , Magnoliopsida/crescimento & desenvolvimento , Óvulo Vegetal/crescimento & desenvolvimento , Reprodução/genética , Sementes/crescimento & desenvolvimento , Zea mays/genética , Zea mays/crescimento & desenvolvimento
4.
PLoS Genet ; 16(4): e1008462, 2020 04.
Artigo em Inglês | MEDLINE | ID: mdl-32236090

RESUMO

In flowering plants, gene expression in the haploid male gametophyte (pollen) is essential for sperm delivery and double fertilization. Pollen also undergoes dynamic epigenetic regulation of expression from transposable elements (TEs), but how this process interacts with gene expression is not clearly understood. To explore relationships among these processes, we quantified transcript levels in four male reproductive stages of maize (tassel primordia, microspores, mature pollen, and sperm cells) via RNA-seq. We found that, in contrast with vegetative cell-limited TE expression in Arabidopsis pollen, TE transcripts in maize accumulate as early as the microspore stage and are also present in sperm cells. Intriguingly, coordinate expression was observed between highly expressed protein-coding genes and their neighboring TEs, specifically in mature pollen and sperm cells. To investigate a potential relationship between elevated gene transcript level and pollen function, we measured the fitness cost (male-specific transmission defect) of GFP-tagged coding sequence insertion mutations in over 50 genes identified as highly expressed in the pollen vegetative cell, sperm cell, or seedling (as a sporophytic control). Insertions in seedling genes or sperm cell genes (with one exception) exhibited no difference from the expected 1:1 transmission ratio. In contrast, insertions in over 20% of vegetative cell genes were associated with significant reductions in fitness, showing a positive correlation of transcript level with non-Mendelian segregation when mutant. Insertions in maize gamete expressed2 (Zm gex2), the sole sperm cell gene with measured contributions to fitness, also triggered seed defects when crossed as a male, indicating a conserved role in double fertilization, given the similar phenotype previously demonstrated for the Arabidopsis ortholog GEX2. Overall, our study demonstrates a developmentally programmed and coordinated transcriptional activation of TEs and genes in pollen, and further identifies maize pollen as a model in which transcriptomic data have predictive value for quantitative phenotypes.


Assuntos
Elementos de DNA Transponíveis/genética , Regulação da Expressão Gênica de Plantas , Aptidão Genética , Pólen/genética , Transcrição Gênica , Zea mays/genética , Linhagem da Célula , Perfilação da Expressão Gênica , Genes de Plantas/genética , Genoma de Planta/genética , Meiose , Mutagênese Insercional , Mutação , Polinização , Reprodutibilidade dos Testes , Reprodução , Sementes/genética , Sementes/crescimento & desenvolvimento , Regulação para Cima , Zea mays/citologia , Zea mays/crescimento & desenvolvimento
5.
Methods Mol Biol ; 1933: 67-86, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30945179

RESUMO

The explosion of RNA-Seq data has enabled the identification of expressed genes without relying on gene models with biases toward open reading frames, allowing the identification of many more long noncoding RNAs (lncRNAs) in eukaryotes. Various tissue enrichment strategies and deep sequencing have also enabled the identification of an extensive list of genes expressed in maize gametophytes, tissues that are intractable to both traditional genetic and gene expression analyses. However, the function of very few genes from the lncRNA and gametophyte sets (or from their intersection) has been tested. Methods for isolating and identifying lncRNAs from gametophyte samples of maize are described here. This method is transferable to any maize gametophyte mutant enabling the development of gene networks involving both protein-coding genes and lncRNAs. Additionally, these methods can be adapted to apply to other grass model systems to test for evolutionary conservation of lncRNA expression patterns.


Assuntos
Perfilação da Expressão Gênica/métodos , Genes de Plantas/genética , Células Germinativas Vegetais/metabolismo , RNA Longo não Codificante/genética , RNA de Plantas/genética , RNA de Plantas/isolamento & purificação , Zea mays/genética , Biologia Computacional/métodos , Regulação da Expressão Gênica de Plantas , Redes Reguladoras de Genes , Células Germinativas Vegetais/crescimento & desenvolvimento , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Transcriptoma , Zea mays/crescimento & desenvolvimento
6.
Genome Biol ; 19(1): 122, 2018 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-30134966

RESUMO

The original version [1] of this article unfortunately contained a mistake. The additive effects of the eQTLs of lncRNAs were flipped, meaning that the base allele in the contrast to derive the additive effects should have been B73, rather than Mo17, due to the original coding of biallele SNPs as "0s" and "1s". Going through the entire analysis procedure, it was determined that the mistake was made while tabulating the eQTL results from QTL Cartographer.

7.
Methods Mol Biol ; 1669: 95-101, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28936653

RESUMO

The plant life cycle is characterized by the alternation of generations between genetically active diploid sporophytes and haploid gametophytes. The gametophytes of flowering plants are sexually dimorphic. While the male gametophyte consists of only three cells (two sperm and a vegetative cell) and is released by the parent sporophyte, the female gametophyte (or embryo sac) is more complex and remains imbedded within diploid sporophyte tissues. In maize, the female gametophyte is embedded in a large ovule surrounded with multiple nucellar cell layers impeding live-cell imaging approaches to study embryo sac functions. Here, we describe a simple protocol to visualize embryo sacs with hormonal fluorescent reporters by increasing accessibility of the female gametophyte. The method described is applicable for visualization of any fluorescent embryo sac reporter. The embryo sacs visualization method developed for maize could be extended to facilitate visualization of embryos sac in other important cereals like wheat, rice, and oats.


Assuntos
Citocininas/metabolismo , Ácidos Indolacéticos/metabolismo , Imagem Óptica/métodos , Óvulo Vegetal/metabolismo , Zea mays/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Células Germinativas Vegetais , Transdução de Sinais/fisiologia
8.
Genetics ; 204(1): 221-31, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27440865

RESUMO

Parent-of-origin-effect loci have non-Mendelian inheritance in which phenotypes are determined by either the maternal or paternal allele alone. In angiosperms, parent-of-origin effects can be caused by loci required for gametophyte development or by imprinted genes needed for seed development. Few parent-of-origin-effect loci have been identified in maize (Zea mays) even though there are a large number of imprinted genes known from transcriptomics. We screened rough endosperm (rgh) mutants for parent-of-origin effects using reciprocal crosses with inbred parents. Six maternal rough endosperm (mre) and three paternal rough endosperm (pre) mutants were identified with three mre loci mapped. When inherited from the female parent, mre/+ seeds reduce grain fill with a rough, etched, or pitted endosperm surface. Pollen transmission of pre mutants results in rgh endosperm as well as embryo lethality. Eight of the mutants had significant distortion from the expected one-to-one ratio for parent-of-origin effects. Linked markers for mre1, mre2, and mre3 indicated that the mutant alleles have no bias in transmission. Histological analysis of mre1, mre2, mre3, and pre*-949 showed altered timing of starch grain accumulation and basal endosperm transfer cell layer (BETL) development. The mre1 locus delays BETL and starchy endosperm development, while mre2 and pre*-949 cause ectopic starchy endosperm differentiation. We conclude that many parent-of-origin effects in maize have incomplete penetrance of kernel phenotypes and that there is a large diversity of endosperm developmental roles for parent-of-origin-effect loci.


Assuntos
Endosperma/genética , Zea mays/genética , Alelos , Perfilação da Expressão Gênica , Genes de Plantas/genética , Loci Gênicos , Impressão Genômica , Mutação , Fenótipo , Sementes/genética , Análise de Sequência de RNA
9.
Genetics ; 204(1): 233-48, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27466227

RESUMO

Flowering plants, like placental mammals, have an extensive maternal contribution toward progeny development. Plants are distinguished from animals by a genetically active haploid phase of growth and development between meiosis and fertilization, called the gametophyte. Flowering plants are further distinguished by the process of double fertilization that produces sister progeny, the endosperm and the embryo, of the seed. Because of this, there is substantial gene expression in the female gametophyte that contributes to the regulation of growth and development of the seed. A primary function of the endosperm is to provide growth support to its sister embryo. Several mutations in Zea mays subsp. mays have been identified that affect the contribution of the mother gametophyte to the seed. The majority affect both the endosperm and the embryo, although some embryo-specific effects have been observed. Many alter the pattern of expression of a marker for the basal endosperm transfer layer, a tissue that transports nutrients from the mother plant to the developing seed. Many of them cause abnormal development of the female gametophyte prior to fertilization, revealing potential cellular mechanisms of maternal control of seed development. These effects include reduced central cell size, abnormal architecture of the central cell, abnormal numbers and morphology of the antipodal cells, and abnormal egg cell morphology. These mutants provide insight into the logic of seed development, including necessary features of the gametes and supporting cells prior to fertilization, and set up future studies on the mechanisms regulating maternal contributions to the seed.


Assuntos
Zea mays/crescimento & desenvolvimento , Zea mays/genética , Arabidopsis/genética , Fertilização , Regulação da Expressão Gênica de Plantas , Células Germinativas Vegetais , Mutação , Óvulo Vegetal , Sementes/genética , Sementes/crescimento & desenvolvimento
10.
Front Plant Sci ; 6: 187, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25859254

RESUMO

The plant life cycle alternates between two genetically active generations: the diploid sporophyte and the haploid gametophyte. In angiosperms the gametophytes are sexually dimorphic and consist of only a few cells. The female gametophyte, or embryo sac, is comprised of four cell types: two synergids, an egg cell, a central cell, and a variable number of antipodal cells. In some species the antipodal cells are indistinct and fail to proliferate, so many aspects of antipodal cell function and development have been unclear. In maize and many other grasses, the antipodal cells proliferate to produce a highly distinct cluster at the chalazal end of the embryo sac that persists at the apex of the endosperm after fertilization. The antipodal cells are a site of auxin accumulation in the maize embryo sac. Analysis of different families of genes involved in auxin biosynthesis, distribution, and signaling for expression in the embryo sac demonstrates that all steps are expressed within the embryo sac. In contrast to auxin signaling, cytokinin signaling is absent in the embryo sac and instead occurs adjacent to but outside of the antipodal cells. Mutant analysis shows a correlation between a loss of auxin signaling and a loss of proliferation of the antipodal cells. The leaf polarity mutant Laxmidrib1 causes a lack of antipodal cell proliferation coupled with a loss of DR5 and PIN1a expression in the antipodal cells.

11.
Genome Biol ; 15(2): R40, 2014 Feb 27.
Artigo em Inglês | MEDLINE | ID: mdl-24576388

RESUMO

BACKGROUND: Long non-coding RNAs (lncRNAs) are transcripts that are 200 bp or longer, do not encode proteins, and potentially play important roles in eukaryotic gene regulation. However, the number, characteristics and expression inheritance pattern of lncRNAs in maize are still largely unknown. RESULTS: By exploiting available public EST databases, maize whole genome sequence annotation and RNA-seq datasets from 30 different experiments, we identified 20,163 putative lncRNAs. Of these lncRNAs, more than 90% are predicted to be the precursors of small RNAs, while 1,704 are considered to be high-confidence lncRNAs. High confidence lncRNAs have an average transcript length of 463 bp and genes encoding them contain fewer exons than annotated genes. By analyzing the expression pattern of these lncRNAs in 13 distinct tissues and 105 maize recombinant inbred lines, we show that more than 50% of the high confidence lncRNAs are expressed in a tissue-specific manner, a result that is supported by epigenetic marks. Intriguingly, the inheritance of lncRNA expression patterns in 105 recombinant inbred lines reveals apparent transgressive segregation, and maize lncRNAs are less affected by cis- than by trans-genetic factors. CONCLUSIONS: We integrate all available transcriptomic datasets to identify a comprehensive set of maize lncRNAs, provide a unique annotation resource of the maize genome and a genome-wide characterization of maize lncRNAs, and explore the genetic control of their expression using expression quantitative trait locus mapping.


Assuntos
Genoma de Planta , RNA Longo não Codificante/genética , Transcrição Gênica , Zea mays/genética , Bases de Dados Genéticas , Éxons , Regulação da Expressão Gênica de Plantas , Anotação de Sequência Molecular , Fases de Leitura Aberta , RNA Longo não Codificante/isolamento & purificação
12.
Plant Reprod ; 27(1): 19-29, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24193168

RESUMO

Three genetic systems conferring cross-incompatibility have been described in Zea mays: Teosinte crossing barrier1-strong (Tcb1-s) found in teosinte, and Gametophyte factor1-strong (Ga1-s) and Ga2-s found in maize and teosinte. The reproductive barrier between maize and some weedy teosintes is controlled by the Tcb1-s locus. Multi-generation inheritance experiments on two independent Tcb1-s lineages show that the Tcb1-s barrier is unstable in some maize lines. Reciprocal crosses between Tcb1-s tester plants and three recombinants in the Tcb1-s mapping region demonstrate that the Tcb1-s haplotype contains separable male and female components. In vivo assays of the dynamics of pollen tube growth and pollen tube morphology during rejection of incompatible pollen in silks carrying the Tcb1-s, Ga1-s, or Ga2-s barriers showed that, in all three, pollen tube growth is slower than in compatible crosses at early stages and had ceased by 24 h after pollination. In all three crossing barrier systems, incompatible pollen tubes have clustered callose plugs in contrast to pollen tubes of compatible crosses. Incompatible pollen tubes growing in the Tcb1-s, Ga1-s, and Ga2-s silks have different morphologies: straight, curved, and kinked, respectively. The distinct morphologies suggest that these crossing barriers block incompatible pollen through different mechanisms. This study lays the foundation for cloning the Tcb1 genes and provides clues about the cellular mechanisms involved in pollen rejection in the Tcb1-s, Ga1-s, and Ga2-s crossing barriers.


Assuntos
Proteínas de Plantas/genética , Zea mays/genética , Cruzamento , Cruzamentos Genéticos , Proteínas de Plantas/metabolismo , Tubo Polínico/citologia , Tubo Polínico/genética , Tubo Polínico/crescimento & desenvolvimento , Tubo Polínico/fisiologia , Polinização , Reprodução , Sementes/citologia , Sementes/genética , Sementes/crescimento & desenvolvimento , Sementes/fisiologia , Autofertilização , Zea mays/citologia , Zea mays/crescimento & desenvolvimento , Zea mays/fisiologia
13.
Genetics ; 187(4): 1085-97, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21270392

RESUMO

Many higher eukaryotes have evolved strategies for the maternal control of growth and development of their offspring. In higher plants this is achieved in part by postmeiotic gene activity controlling the development of the haploid female gametophyte. stunter1 (stt1) is a novel, recessive, maternal effect mutant in maize that displays viable, miniature kernels. Maternal inheritance of stt1 results in seeds with reduced but otherwise normal endosperms and embryos. The stt1 mutation displays reduced transmission through the male and female parents and causes significant changes in the sizes of both male and female gametophytes. stt1 pollen grains are smaller than wild type, have reduced germination efficiency, and reduced pollen tube growth. stt1 embryo sacs have smaller central cells and abnormal antipodal cells that are larger, more vacuolated, and fewer in number than wild type. Embryos and endosperms produced by fertilization of stt1 embryo sacs develop and grow more slowly than wild type. The data suggest that the morphology of mutant embryo sacs influences endosperm development, leading to the production of miniature kernels in stt1. Analysis of seeds carrying a mutant maternal allele of stt1 over a deletion of the paternal allele demonstrates that both parental alleles are active after fertilization in both the endosperm and embryo. This analysis also indicates that embryo development until the globular stage in maize can proceed without endosperm development and is likely supported directly by the diploid mother plant.


Assuntos
Genes de Plantas , Células Germinativas Vegetais/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento , Zea mays/genética , Alelos , Mapeamento Cromossômico , Endosperma/embriologia , Endosperma/genética , Fertilização , Regulação da Expressão Gênica no Desenvolvimento , Regulação da Expressão Gênica de Plantas , Germinação , Mutação INDEL , Modelos Biológicos , Mutação , Fenótipo , Pólen/embriologia , Pólen/genética , Sementes/embriologia , Sementes/genética , Zea mays/embriologia , Zea mays/crescimento & desenvolvimento
14.
J Hered ; 101(6): 737-49, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20696670

RESUMO

Major genes govern the fertilization of teosinte ovules by maize pollen. A pollen-pistil compatibility system different from the previously described systems, Ga1-s and Tcb1-s, was identified among maize lines introgressed with chromosome segments from 2 teosinte populations. The pistil barrier is dominant, and pollen competence is determined by genotype of the individual pollen grain. A major gene governing this incompatibility behaves as a strong allele of ga2, a locus identified previously among maize genetic stocks on the basis of transmission ratio distortion. Additionally, pollen simultaneously carrying both ga2 and Ga2 was functional on Ga2 silks, which have the pistil barrier, indicating that Ga2 conditions acceptance of the pollen grain rather than ga2 conditioning rejection of the pollen grain by Ga2 silks. The strong allele (Ga2-s), a weaker one such as reported among maize genetic stocks (Ga2-w), and an allele having only pollen competence (Ga2-m), or some combination of these, was found in all 13 of the teosinte populations sampled. Sympatric and parapatric maize landraces carried Ga2-m or the presumed null allele ga2, but Ga2-s or Ga2-w was not found. The combination of exclusively Ga2-s teosinte with ga2 maize, which could provide strong reproductive isolation, was not characteristic of the 5, paired populations tested.


Assuntos
Cruzamento , Genes de Plantas , Polinização , Zea mays/genética , Zea mays/fisiologia , Alelos , Cruzamentos Genéticos , Flores/genética , Flores/fisiologia , Variação Genética , Genótipo , Endogamia , Óvulo Vegetal/genética , Óvulo Vegetal/fisiologia , Fenótipo , Pólen/genética , Pólen/fisiologia , Reprodução/genética , Reprodução/fisiologia , Sementes/genética , Sementes/fisiologia
15.
Plant Cell ; 19(11): 3379-90, 2007 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18055602

RESUMO

The Arabidopsis thaliana REVOLUTA (REV) protein is a member of the class III homeodomain-leucine zipper (HD-ZIPIII) proteins. REV is a potent regulator of leaf polarity and vascular development. Here, we report the identification of a gene family that encodes small leucine zipper-containing proteins (LITTLE ZIPPER [ZPR] proteins) where the leucine zipper is similar to that found in REV, PHABULOSA, and PHAVOLUTA proteins. The transcript levels of the ZPR genes increase in response to activation of a steroid-inducible REV protein. We show that the ZPR proteins interact with REV in vitro and that ZPR3 prevents DNA binding by REV in vitro. Overexpression of ZPR proteins in Arabidopsis results in phenotypes similar to those seen when HD-ZIPIII function is reduced. We propose a negative feedback model in which REV promotes transcription of the ZPR genes. The ZPR proteins in turn form heterodimers with the REV protein, preventing it from binding DNA. The HD-ZIPIII/ZPR regulatory module would serve not only to dampen the effect of fluctuations in HD-ZIPIII protein levels but more importantly would provide a potential point of regulation (control over the ratio of inactive heterodimers to active homodimers) that could be influenced by other components of the pathway governing leaf polarity.


Assuntos
Proteínas de Arabidopsis/metabolismo , Arabidopsis/genética , Retroalimentação Fisiológica , Genes de Plantas , Sequência de Aminoácidos , Arabidopsis/citologia , Arabidopsis/efeitos dos fármacos , Proteínas de Arabidopsis/química , Dexametasona/farmacologia , Retroalimentação Fisiológica/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Proteínas de Homeodomínio/metabolismo , Dados de Sequência Molecular , Folhas de Planta/citologia , Folhas de Planta/efeitos dos fármacos , Ligação Proteica/efeitos dos fármacos
16.
Plant Cell ; 19(1): 46-62, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-17209126

RESUMO

Angiosperm embryo sac development begins with a phase of free nuclear division followed by cellularization and differentiation of cell types. The indeterminate gametophyte1 (ig1) gene of maize (Zea mays) restricts the proliferative phase of female gametophyte development. ig1 mutant female gametophytes have a prolonged phase of free nuclear divisions leading to a variety of embryo sac abnormalities, including extra egg cells, extra polar nuclei, and extra synergids. Positional cloning of ig1 was performed based on the genome sequence of the orthologous region in rice. ig1 encodes a LATERAL ORGAN BOUNDARIES domain protein with high similarity to ASYMMETRIC LEAVES2 of Arabidopsis thaliana. A second mutant allele of ig1 was identified in a noncomplementation screen using active Mutator transposable element lines. Homozygous ig1 mutants have abnormal leaf morphology as well as abnormal embryo sac development. Affected leaves have disrupted abaxial-adaxial polarity and fail to repress the expression of meristem-specific knotted-like homeobox (knox) genes in leaf primordia, causing a proliferative, stem cell identity to persist in these cells. Despite the superficial similarity of ig1-O leaves and embryo sacs, ectopic knox gene expression cannot be detected in ig1-O embryo sacs.


Assuntos
Proteínas de Plantas/fisiologia , Sementes/crescimento & desenvolvimento , Zea mays/embriologia , Zea mays/crescimento & desenvolvimento , Sequência de Aminoácidos , Proteínas de Arabidopsis/química , Proliferação de Células , Mapeamento Cromossômico , Clonagem Molecular , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Dados de Sequência Molecular , Família Multigênica , Mutação , Fenótipo , Filogenia , Folhas de Planta/citologia , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/genética , Estrutura Terciária de Proteína , RNA Mensageiro/metabolismo , Sementes/citologia , Sementes/metabolismo , Alinhamento de Sequência , Células-Tronco/citologia , Células-Tronco/metabolismo , Fatores de Transcrição/química , Zea mays/genética
17.
Genetics ; 174(1): 317-29, 2006 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-16849604

RESUMO

In angiosperms, double fertilization of an egg cell and a central cell with two sperm cells results in the formation of a seed containing a diploid embryo and a triploid endosperm. The extent to which the embryo sac controls postfertilization events in the seed is unknown. The novel gametophytic maternal-effect maize mutation, baseless1 (bsl1) affects central cell development within the embryo sac, frequently by altering the position of the two polar nuclei. Despite this irregularity, fertilization is as efficient as in wild type. The spatial expression of basal endosperm-specific transcripts is altered in free-nuclear and cellular mutant endosperms. At later stages of seed development, bsl1 predominantly affects development of the basal endosperm transfer layer (BETL). When bsl1/+ diploid plants were pollinated by wild-type tetraploid plants, the BETL abnormalities observed in bsl1/bsl1/+/+ tetraploid endosperms were diverse and of variable severity. Moreover, the frequency of kernels with severely perturbed BETL development correlated with the percentage of severely affected bsl1 central cells. Therefore, BSL1 is likely required in the central cell before fertilization for correct BETL patterning to occur. These findings provide new genetic evidence that a maternal gametophytic component is necessary for correct endosperm patterning.


Assuntos
Genes de Plantas/fisiologia , Fenótipo , Zea mays/embriologia , Zea mays/crescimento & desenvolvimento , Zea mays/genética , Gametogênese/genética , Regulação da Expressão Gênica de Plantas , Modelos Biológicos , Mutação/fisiologia , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Pólen/embriologia , Pólen/genética , Sementes/embriologia , Sementes/genética , Distribuição Tecidual
18.
Nat Rev Genet ; 4(5): 369-79, 2003 May.
Artigo em Inglês | MEDLINE | ID: mdl-12728279

RESUMO

Continuous development, the absence of a germline, flexible and reversible cellular differentiation, and the existence of haploid and diploid generations--both of which express genes--are characteristics that distinguish plants from animals. Because these differences alter the impact of mutations, animals and plants experience varied selection pressures. Despite different life-cycles, both flowering plants and multicellular animals have evolved complex sensing mechanisms that act after fertilization as 'quality checks' on reproduction, and that detect chromosome dosage and the parent of origin for specific genes. Although flowering plant embryos escape such surveillance in vitro, embryo success in the seed often depends on a healthy endosperm--a nutritive tissue that is produced by a second fertilization event in which maternal and paternal gene contributions can be monitored immediately after fertilization and throughout development.


Assuntos
Estágios do Ciclo de Vida/fisiologia , Desenvolvimento Vegetal , Animais , Genes de Plantas/fisiologia
19.
Artigo em Inglês | MEDLINE | ID: mdl-15012278

RESUMO

Recent progress has been made in the genetic dissection of angiosperm shoot apical meristem (SAM) structure and function. Genes required for proper SAM development have been identified in a variety of species through the isolation of mutants. In addition, genes with expression patterns indicating they play a role in SAM function have been identified molecularly. The processes of SAM formation, self-renewal, and pattern formation within the SAM are examined with an emphasis on the contributions of recent classical and molecular genetic experiments to our understanding of this basic problem in plant developmental biology.

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