RESUMO
Acrosin activity is influenced by various inhibitors and stimulators. The present study investigated the ability of tubular and follicular fluid, taken at different stages of the menstrual cycle, to stimulate or inhibit acrosin activity. Endometrial histology and cervical mucus microscopy were used to ascertain the stage of the menstrual cycle. Normal human sperm were incubated separately with tubular and follicular fluid and then assessed for gross motility and acrosin activity. The fluids were also investigated immunoelectrophoretically against anti-alpha 1-antitrypsin and anti-alpha-2-macroglobulin. Nonovulatory tubular fluid inhibited acrosin activity, while ovulatory tubular fluid and that from the late proliferative stages lacked inhibition. Ovulatory follicular fluid acrosin activity, while nonovulatory follicular fluid did not. Ovulatory fluid also enhanced sperm motility. Immunoelectrophoresis of tubular fluid suggests the presence of some other potential inhibitors in addition to anti-alpha 1-antitrypsin. It would appear that tubular fluid maintains rather than activates sperm function, while follicular fluid at ovulation stimulates acrosin proteolytic activity and sperm motility.
Assuntos
Acrosina/metabolismo , Líquidos Corporais/fisiologia , Tubas Uterinas , Líquido Folicular/fisiologia , Ciclo Menstrual/fisiologia , Motilidade dos Espermatozoides/fisiologia , Acrosina/antagonistas & inibidores , Ativação Enzimática , Feminino , Humanos , MasculinoRESUMO
High titre antiserum was obtained by immunization of rabbits with phalacidine-concanavaline A. Specific antiphalacidine antibodies were isolated from this serum on immobilized phalacidine. It was established by testing with antirabbit globulin sera that these antibodies were of IgG class only. IgG fraction was isolated from the immune serum by ion-exchange chromatography, but specific antibodies, which represented 8% of IgG of the serum, were isolated from it by affinity chromatography. The antibodies isolated by affinity, were tested against other phalatoxines and amotoxines. The results showed that they reacted with phalatoxines, but not with amatoxines and could be used in tests for quantitative determination of phalatoxines in mushroom extracts and body fluids.
Assuntos
Amanitinas/imunologia , Anticorpos/análise , Especificidade de Anticorpos , Concanavalina A/imunologia , Imunização/métodos , Peptídeos Cíclicos/imunologia , Animais , Cromatografia de Afinidade/métodos , Cromatografia em Gel/métodos , Imunodifusão/métodos , Masculino , CoelhosRESUMO
The patients was a 16-year old girl with the spinal muscular atrophy. Clinical laboratory and genetic investigations were performed. A close relative of the proband suffering from the same ailment was found in analysing the genealogic tree. Enzymatic study (creatine phosphokinase, lactic dehydrogenase) of the proband and her parents proved the autosome-recessive type of inheriting the disorder.
Assuntos
Atrofia Muscular Espinal/genética , Adolescente , Feminino , Humanos , Atrofia Muscular Espinal/classificação , Atrofia Muscular Espinal/diagnóstico , Linhagem , Terminologia como AssuntoRESUMO
The isoenzyme polymorphism and kinetics of glucose-6-phosphate dehydrogenase (G6PD) in embryonal and mature brain were studied. Two types of electropherograms were found in embryonal brains: type I is characterized by two G6PD fractions: slow and fast, and in the type II a single, fast-moving G6PD fraction was seen in a single embryo. The additional studies carried out, namely reelectrophoresis following 2-mercaptoethanol and NADP treatment and the specific enzyme kinetics, i.e. altered Michaelis constant and enhanced utilization of the desamino-NADP analogue which are typical of the fast-moving fraction in embryonal brain, speak unequivocally in favor of the presence of a new, unknown, phase-specific isoenzyme of G6PD. Its synthesis takes place only during embryonal brain development. A probable autosomal localization for the gene of brain G6PD is suggested.
