RESUMO
The steroidal saponins of Tribulus terrestris L. (Zygophyllaceae) are considered to be the factor responsible for biological activity of products derived from this plant. The activity depends on the concentration and the composition of active saponins, which in turn is influenced by the geographical origin of plant material. Samples of T. terrestris collected in Bulgaria, Greece, Serbia, Macedonia, Turkey, Georgia, Iran, Vietnam and India were analyzed by LC-ESI/MS/MS for the presence and the concentration of protodioscin (1), prototribestin (2), pseudoprotodioscin (3), dioscin (4), tribestin (5) and tribulosin (6). The flavonoid rutin (7) was also included in the comparison. The results revealed distinct differences in the content of these compounds depending on region of sample collection, plant part studied and stage of plant development. The samples from Bulgaria, Turkey, Greece, Serbia, Macedonia, Georgia and Iran exhibited similar chemical profile and only some quantitative difference in the content of 1-7 with protodioscin (1) and prototribestin (2) as main components. The Vietnamese and Indian samples exhibit totally different chemical profile. They lack 2 and 5, while tribulosin (6) is present in high amounts. Compounds different from 1 to 7 are dominating in these 3 samples. The presented results suggested the existence of one chemotype common to the East South European and West Asian regions. Most probably, the Vietnamese and Indian samples belong to other chemotypes which are still to be studied and characterized. No clear correlation between the burrs morphology and the chemical composition of the samples has been found.
Assuntos
Geografia , Saponinas/análise , Saponinas/química , Esteroides/química , Tribulus/química , Ásia Ocidental , Cromatografia Líquida de Alta Pressão , Cromatografia em Camada Fina , Europa (Continente) , Componentes Aéreos da Planta/química , Espectrometria de Massas por Ionização por Electrospray , Esteroides/análiseRESUMO
The new alkaloid 6alpha-angeloylplatynecine, together with other four known pyrrolizidine alkaloids was identified from above ground parts of Senecio nemorensis subs. fuchsii.
Assuntos
Alcenos/química , Compostos Heterocíclicos com 2 Anéis/química , Senécio/química , Estrutura Molecular , Componentes Aéreos da Planta/químicaRESUMO
Twenty-seven pyrrolizidine alkaloids were identified by a detailed phytochemical study and GC/MS analysis on twelve Bulgarian species from genus Senecio. Twenty of them are new for the corresponding species. Other six structures remain tentatively identified.
Assuntos
Alcaloides de Pirrolizidina/química , Senécio/química , Bulgária , Alcaloides de Pirrolizidina/isolamento & purificação , Senécio/classificaçãoRESUMO
The new alkaloids 7-,9-diangeloylplatynecine (1) and 8-episarracine N-oxide (2), were isolated and identified from the roots of Senecio macedonicus. Another one, 8-epineosarracine was detected by GC/MS analyses of the crude alkaloid mixture. The cytotoxicity and biological activity of the alkaloids were tested on normal murine spleen lymphocytes and P3U1 mouse myeloma.
