Estudo comparativo entre esfregaços de punção esplênica e de sangue periférico para diagnóstico de babesiose equina / Comparative study of smears of spleen and peripheral blood for diagnosis of babesiosis in horses

O objetivo deste trabalho foi o de comparar os resultados obtidos com o uso das técnicas de esfregaço de sangue periférico e de punção esplênica e de verificar uma metodologia simples e precisa para diagnóstico de babesioses em cavalos portadores subclínicos, submetidos ao esforço físico ou competições. Quinze equinos hígidos, sem sinais clínicos de babesiose, com peso médio de 350 Kg, idade entre 6 a 13 anos, de ambos os sexos e com histórico de infestação por carrapatos, foram utilizados. No esfregaço de sangue colhido da orelha, nenhum animal apresentou resultado positivo enquanto que na punção esplênica cinco animais foram positivos para a presença de Babesia caballi ou Theileria equi. Os dois métodos são de fácil realização, seguros e pouco invasivos, porém o esfregaço da punção esplênica parece ser mais eficaz em detectar casos latentes de babesiose em equinos

The objective of this work was to compare the efficacy of these two techniques and to search for a simple and more precise diagnostic method for subclinical babesiosis in horses submitted to physical effort or competitions. Then, splenic punction and periferic blood smear were compared. Fifteen healthy horses, without clinical signs of babesiosis, median weight 350 Kg, age between six to thirteen years, both genders and with an history of tick infestation were used. Five animals were positive for Babesia caballi or Theileria equi, in splenic punction and none in blood smear. Both methods were little invasive, safe and easy to do, but the splenic punction smear seems to be more efficace to detect cronic babesioses in horses

COMPARATIVE STUDY OF SPLENIC PUNCTURE AND PERIPHERAL BLOOD SMEARS FOR DIAGNOSIS OF BABESIOSIS IN HORSES / Estudo comparativo entre esfregaços de punção esplênica e sangue periférico para diagnóstico de babesiose equina

O objetivo deste trabalho foi o de comparar os resultados obtidos com o uso das técnicas de esfregaço de sangue periférico e de punção esplênica e de verificar uma metodologia simples e precisa para diagnóstico de babesioses em cavalos portadores subclínicos, submetidos ao esforço físico ou competições. Quinze equinos hígidos, sem sinais clínicos de babesiose, com peso médio de 350 Kg, idade entre 6 a 13 anos, de ambos os sexos e com histórico de infestação por carrapatos, foram utilizados. No esfregaço de sangue colhido  da orelha, nenhum animal apresentou resultado positivo enquanto que na punção esplênica cinco animais foram positivos para a presença de Babesia caballi ou Theileria equi. Os dois métodos são de fácil realização, seguros e pouco invasivos, porém o esfregaço da punção esplênica parece ser mais eficaz em detectar casos latentes de babesiose em equinos.   SUMMARY The objective of this work is to compare the efficacy of the splenic puncture and peripheral blood smear techniqu

Estudo comparativo entre esfregaços de punção esplênica e de sangue periférico para diagnóstico de babesiose equina / Comparative study of smears of spleen and peripheral blood for diagnosis of babesiosis in horses

O objetivo deste trabalho foi o de comparar os resultados obtidos com o uso das técnicas de esfregaço de sangue periférico e de punção esplênica e de verificar uma metodologia simples e precisa para diagnóstico de babesioses em cavalos portadores subclínicos, submetidos ao esforço físico ou competições. Quinze equinos hígidos, sem sinais clínicos de babesiose, com peso médio de 350 Kg, idade entre 6 a 13 anos, de ambos os sexos e com histórico de infestação por carrapatos, foram utilizados. No esfregaço de sangue colhido da orelha, nenhum animal apresentou resultado positivo enquanto que na punção esplênica cinco animais foram positivos para a presença de Babesia caballi ou Theileria equi. Os dois métodos são de fácil realização, seguros e pouco invasivos, porém o esfregaço da punção esplênica parece ser mais eficaz em detectar casos latentes de babesiose em equinos(AU)

The objective of this work was to compare the efficacy of these two techniques and to search for a simple and more precise diagnostic method for subclinical babesiosis in horses submitted to physical effort or competitions. Then, splenic punction and periferic blood smear were compared. Fifteen healthy horses, without clinical signs of babesiosis, median weight 350 Kg, age between six to thirteen years, both genders and with an history of tick infestation were used. Five animals were positive for Babesia caballi or Theileria equi, in splenic punction and none in blood smear. Both methods were little invasive, safe and easy to do, but the splenic punction smear seems to be more efficace to detect cronic babesioses in horses(AU)

COMPARATIVE STUDY OF SPLENIC PUNCTURE AND PERIPHERAL BLOOD SMEARS FOR DIAGNOSIS OF BABESIOSIS IN HORSES / Estudo comparativo entre esfregaços de punção esplênica e sangue periférico para diagnóstico de babesiose equina.

O objetivo deste trabalho foi o de comparar os resultados obtidos com o uso das técnicas de esfregaço de sangue periférico e de punção esplênica e de verificar uma metodologia simples e precisa para diagnóstico de babesioses em cavalos portadores subclínicos, submetidos ao esforço físico ou competições. Quinze equinos hígidos, sem sinais clínicos de babesiose, com peso médio de 350 Kg, idade entre 6 a 13 anos, de ambos os sexos e com histórico de infestação por carrapatos, foram utilizados. No esfregaço de sangue colhido  da orelha, nenhum animal apresentou resultado positivo enquanto que na punção esplênica cinco animais foram positivos para a presença de Babesia caballi ou Theileria equi. Os dois métodos são de fácil realização, seguros e pouco invasivos, porém o esfregaço da punção esplênica parece ser mais eficaz em detectar casos latentes de babesiose em equinos.   SUMMARY The objective of this work is to compare the efficacy of the splenic puncture and peripheral blood smear techniqu

Congenital papillomas and papillomatoses associated with the human papilloma virus (HPV)-report on 5 cases.

The authors present a study of five cases of vulvar congenital papillomas and papillomatoses in stillborns and neonates dead upon birth. The studied material was collected from five necropsies. The histopathological evaluation showed hyperkeratosis, acanthosis, papillomatosis, perinuclear haloes, and nuclear abnormalities. In three of the cases, the electron microscopy identified nuclear and cytoplasmatic viral particles ranging from 40 to 60 nm in size, compatible with HPV. The immunohistochemical study of those lesions showed nuclear and cytoplasmatic positivity. The authors concluded that the presence of viral particles suggestive of HPV added to the immunopositivity indicated the possibility of viral infection.

Placental morphology in cytomegalovirus infection.

Three groups of placentae from 27 cases of cytomegalovirus infection were examined morphologically and by specific immunofluorescence. Placentae in Group 1 (four cases) had a mean gestational age of 24.5 +/- 2.5 weeks; those in Group 2 (11 cases) had a mean gestational age of 36.4 +/- 2.7 weeks; those in Group 3 (12 cases) had a mean gestational age of 40 +/- 1.8 weeks. A tendency towards increased placental weight was observed. In 23 cases, a haematogenous placentitis was suspected on gross examination. Some specific features were detected on light microscopy. In Group 1 the lesions were represented by pronounced dysmaturity of villous structures, diffuse reparative villitis and intervillitis allied to an abundance of cytomegalic cells and areas of necrosis and calcification. In Group 2 a chronic active process was indicated by the presence of vascular involvement, proliferative-necrotic and reparative villitis and fresh villous necrosis; cytomegalic cells were scarce. In Group 3 placentae there was a predominance of villous plate lesions similar to those found in the septicaemic phase of maternal infections; necrotizing, proliferative and, rarely, reparative villitis was present; cytomegalic cells were rare. We may conclude that placental morphology needs to be substantiated by special laboratory techniques for the detection of cytomegalovirus infection.

Association between HBsAg and race in a mixed population of Northeastern Brazil.

The frequency of Australian antigen carriers (HBsAg) was studied in three samples from northeastern Brazil. In sample A there were 1000 women from Tsylla Balbino Maternity in Salvador; in sample B there were 1,512 blood donors from Salvador, and in sample C there were 930 blood donors from Aracaju. In sample A, B and C the observed frequency of carriers (HBsAg) was 1.20%, 1.58% and 2.04% respectively. These differences were not significant (x22 = 2.2; p greater than 0.25). There was no association between the carrier state and sex, age, ABO and Rh blood groups, Chagas' disease and rural origin. However, the frequency of carriers (HBsAg) increases from White to Black, as follows: Whites = 0.5%; Mulattoes = 1.91%, and Black = 1.96% (x22 = 7.91; p less than 0.02).

Human aconitase polymorphism in three samples from northeastern Brazil.

Human aconitase (ACONS) polymorphism was studied in three samples from northeastern Brazil. Two of the samples were collected in the State of Bahia and one in the State of Sergipe. The main characteristic of the samples was given by different degrees of Black admixture. The results showed that the more negroid the samples the higher the frequencies of the alleles ACONS4, ACONS2 and ACONS6. These findings fit well with the known ACONS gene frequencies in present-day Nigerians and with the past history of Yoruba slaves in Bahia.