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1.
Environ Sci Pollut Res Int ; 30(47): 103958-103971, 2023 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-37691062

RESUMO

With the growing nature of the ecological footprint, research studies focus on exploring new determinants of environmental degradation. Moreover, the role of natural resources and energy consumption in environmental quality has gained much attention in the literature. However, tourism raises the demand for energy consumption and extraction of natural resources. This research study investigates the influence of natural resources, tourism, and renewable energy in MINT countries, using novel Cross-Sectional Auto Regressive Distributive Lag (CS-ARDL) methodological techniques and employing yearly data from 1995 to 2018. The study also applied recently developed Kónya (Econ Model 23:978-992, 2006) causality to identify the causal relationship between the variables of the heterogenous panel. The result shows that tourism, natural resources, and economic growth are positively associated with the ecological footprint in the long-run. However, renewable energy consumption negatively impacts ecological footprint in both in short-run and the long-run. Further, the study explored a bidirectional causality between economic growth and ecological footprint in MINT countries. Finally, based on the empirical results, the study recommends that the authorities in MINT countries revisit their tourism, natural resources, and economic activities policies to enhance the environmental quality and reduce the ecological footprint.


Assuntos
Dióxido de Carbono , Turismo , Estudos Transversais , Recursos Naturais , Desenvolvimento Econômico , Energia Renovável
2.
Heliyon ; 9(7): e17791, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37483806

RESUMO

This research study examined the influence of financial market development on the shadow economy and the moderating effect of country risk (political, economic, and financial) in this nexus in Pakistan. Using data from 1995 to 2018, the study applied the Augmented Dickey-Fuller (ADF) and Phillips-Perron (PP) unit root tests, followed by the F-bounds test to investigate stationarity and cointegration in the series, respectively. The study utilized the Autoregressive Distributed Lag (ARDL) approach to estimate the long-run relationship, and to examine the possible causal relationship among the variables, the study employed Breitung and Candelon's (2006) spectral test. The study identified that financial market development is negative, and the country's risk determinants are positively associated with the shadow economy's size. Moreover, the study found that country risk positively moderates the influence of financial market development on the shadow economy. The results also highlighted a unidirectional relationship from economic and financial risk towards the shadow economy. Finally, based on the empirical findings, the study recommends some policy implications to the regulators of financial markets and the shadow economy.

3.
Heliyon ; 9(1): e12765, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36685379

RESUMO

Brand attitude has been the primary concern for corporation sustainability for a long. Studies have focused on various attributes concerning brand attitude comprising of many research articles. This study focuses on bibliometric analysis of brand attitude examining 1497 documents published for the years 1944-2021. The purpose of this study is to examine historic research trends, developments, and future conduits based on co-authorship and co-occurrence analysis using VOSviewer. The study highlights the most prolific articles containing the most productive authors and their affiliations, keywords, and influential journals, along with future research trends. The result shows that the number of publications follows a very slow trail until 2008, mildly until 2012, following which the cumulative growth in publication increased manifold. Further, results show that researchers from the United States, South Korea, and Taiwan extended many efforts to the global knowledge of literature leading other countries. The emerging hotspot keywords analysis suggests that brand avoidance, subtle, brand betrayal, consumer ethnocentrism, environmental sustainability and policy, brand activism, brand authenticity, consumer brand engagement, and brand competence are the key areas that attract researchers' attention policymakers as future research boulevards. In the case of consumers' brand attitudes, there is a scarcity of studies that investigated the consumers' implicit and explicit brand attitudes.

4.
J Family Med Prim Care ; 11(8): 4753-4757, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-36353010

RESUMO

Background: A single child is precious for every parent. There is an increasing demand for children to perform and to excel in all aspects of their lives. Due to increased unemployment and less job opportunities, tough competition to get admission in good school and college and to get respectable job put every parent under constant stress. These circumstances lead to parents starting micromanagement of their children. This micromanagement can be harmful to the mental and emotional wellbeing of the child, and is especially seen in single-child parents. Objective: This study aimed to ascertain if parents of single child show behavior of helicopter parenting as compared to patents having more than one child. Methods: This is an open-ended observational study, wherein 100 families with single child and 50 families with more than one child were interviewed based on self-explanatory questionnaire methods. Results: We noted that 83% parents showed behavior of helicopter parenting, especially those who have single child as compared to those having more than one child. Conclusion: Based on our survey, we can conclude that parents of single child showed behavior of helicopter parenting as compared to patents having more than one child because only child is precious and center of attraction in the families especially in Indian culture. However, large studies are required to reach a definitive conclusion.

5.
Int J Gen Med ; 15: 7631-7637, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36226309

RESUMO

Objective: To determine the correlation of first trimester maternal 25-(OH)-D level with postnatal infant growth in Indonesia. Materials and Methods: A cohort of 116 mother-infant pairs from Indonesia was prospectively studied. 25-(OH)-D level was measured in maternal serum at 10-14 weeks of gestation and in umbilical cord blood shortly after birth. The newborns were observed longitudinally for 24 months. Length and head circumference were measured at birth and at ages 3, 6, 12, and 24 months. Spearman correlation and multiple linear regression analyses were performed. Results: Mean 25-(OH)-D levels in the prenatal maternal serum and umbilical cord blood were 17.55 ± 7.33 ng/mL and 16.27 ± 6.14 ng/mL, respectively. Prenatal maternal 25-(OH)-D level weakly correlated with infant length (r = -0.35) and head circumference (r = -0.21) z-scores at age 3 months. Umbilical cord blood 25-(OH)-D level did not correlate with infant length or head circumference at any time point. Multiple linear regression showed an independent association between prenatal maternal 25-(OH)-D level and infant length z-score at age 3 months (p = 0.01, SE ß = 0.02, and coefficient ß = -0.06). Conclusion: First trimester maternal serum 25(OH)D level correlated with infant length and head circumference at age 3 months.

6.
Environ Dev Sustain ; : 1-30, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36039365

RESUMO

The finance and natural resources revenue nexus play a critical role in an economy. The recent development and significant increase in academic literature regarding the resource-finance nexus are the primary motivations for conducting this study. This study aims to conduct a bibliometric analysis of 363 documents published between 1976 and 2021 collected from the Scopus database. The results have been demonstrated via graphs, tables, knowledge maps about the past trends, growth, and prospects using co-occurrence, co-authorship, and co-citation analysis via the VOSviewer tool. This study has identified prolific authors, journals, countries, academic institutions, and future pathways. The findings indicate that China has the highest share of publications (88, 24.2%), followed by Pakistan (58, 15.9%) and Turkey (37, 10.2%). The most productive academic institution is the Beijing Institute of Technology in China (13, 3.6%). This study proposes new avenues for further research concerning the resource-finance nexus, such as ecological footprint, sustainability, fiscal decentralization, green investment, energy prices, environmental quality, technological innovation, financial resource curse (especially the stock market resource curse), human capital, and renewable energy in policy development and sustainability towards the achievement of the SDGs.

7.
Air Qual Atmos Health ; 14(11): 1773-1784, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34093896

RESUMO

This study is an attempt to explain the nexus between health expenditures, GDP, human development index (HDI), CO2 emissions (COEM), renewable energy (RENE), financial development (FD) and electricity consumption (EC) using data from 2000Q1 to 2014Q4 for Brazil, India, China and South Africa. The study applies CIPS and CADF to determine the integration order. The tests confirmed the unique order of integration. The study further uses the Westerlund panel cointegration, which suggests the existence of a long-run relationship. Moreover, the panels dynamic ordinary least squares (DOLS) and fully modified ordinary least squares (FMOLS) are applied to ascertain the long-run elasticity. The health expenditure and electricity consumption affect the COEM positively. Moreover, HDI and RE affect COEM negatively. The study further confirms the existence of an N-shaped EKC in the long run. The pairwise Dumitrescu and Hurlin, Econ Model 29:1450-1460, (2012) test is used to uncover the direction of the association between the variables. The findings obtained from DH confirm a bidirectional causality between HDI and FD. Likewise, another bidirectional causal relationship has also been found between FD and EC. The findings of our study advocate policies in the direction of HDI and health expenditure by adopting RENE. This study highlights the importance of RENE, which can facilitate a reduction in carbon emissions and decreasing health expenditures. Moreover, the financial sector needs to be improved to create entrepreneurship opportunities for the public in improving the HDI in ensuring sustainable development.

8.
Environ Sci Pollut Res Int ; 28(27): 36344-36353, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33694110

RESUMO

The majority of studies investigating the environmental Kuznets curve predominantly focus on atmospheric indicators, thereby neglecting other environmental indicators such as land, sea, coastal, coral reefs, freshwater, and biodiversity indicators. This study aims to examine the environmental Kuznets curve by using capture fisheries production as a biodiversity indicator. The study uses a panel of 14 countries, of which 10 are newly industrialized and the other 4 are fast-emerging countries. The study applies the CADF and CIPS unit root tests to identify the integration order as proposed by Pesaran (2007). After identifying the unique order of integration, the Westerlund (2007) panel cointegration is applied. A long-run relationship is confirmed among the variables. The study revealed that an N-pattern relationship exists between capture fisheries production (CFP) and growth of the economy in the panel of selected countries. The industry focuses on achieving a cleaner environment and promotes the sustainable development of the fisheries. Financial development has a negative and significant effect on CFP. This reflects that domestic credit is not only used for the capture of fish but also for conservation purposes. The exports of goods and services have a positive relationship with CFP, while imports have a negative and significant effect on CFP. Policies to promote investments in the conservation of fisheries should be implemented, and credit creation should be directed by appropriate legislation to ensure the conservation of biodiversity and environmental sustainability.


Assuntos
Dióxido de Carbono , Desenvolvimento Econômico , Animais , Biodiversidade , Pesqueiros , Investimentos em Saúde
9.
Environ Sci Pollut Res Int ; 28(5): 5953-5965, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32981018

RESUMO

This study is conducted to address the research question of whether hydroelectricity and fossil fuels contribute to sustainable economic development in an emerging economy in this era of globalization? Further, this study applies the novel approach of Harvey unit root test which is a linearity test to predict the possible existence of non-linearity. The results confirmed that the majority of the series in this study are linear. Furthermore, the two break test is applied to investigate the integration sequence of the series. The bounds test approach confirms the existence of a long-run association among the variables. Additionally, the long-run relationship is analysed within the framework of the ARDL approach. Financial development, fossil fuel, and capital positively contribute to economic development, while the effect of hydroelectricity is insignificant. Moreover, globalization effects GDP negatively. The symmetric causality suggests a uni-directional causal movement from hydroelectricity consumption and globalization towards GDP. The outcome of the study emphasizes the importance of renewable sources such as hydropower energy for ensuring sustainable development in the presence of globalization.


Assuntos
Desenvolvimento Econômico , Combustíveis Fósseis , Dióxido de Carbono/análise , Internacionalidade , Desenvolvimento Sustentável
10.
Afr J Reprod Health ; 24(1): 53-61, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32358937

RESUMO

This study aimed to describe the current incidence and mortality rates of gynecologic cancer and their association with socio- economic development. The data for the age-standardized incidence rate (ASRI) and age-standardized mortality rate (ASRM) were acquired from the GLOBOCAN-2012 database. Human Development Index (HDI) data were obtained from the 2015- Human Development Report. The correlation between HDI and Mortality to Incidence Ratio (MIR) was assessed by Pearson- correlation. The effect of national-HDI on MIR was analyzed by linear regression analysis. The ASRI, ASRM, and MIR of cervix cancer were higher in the less developed regions (LDRs) than in more developed regions (MDRs). However, for corpus uteri cancer, the ASRI was 3.6 times and the ASRM was 1.5-times higher in the MDRs than in the LDRs. Strong inverse associations between MIR and HDI were reported from cervix (adjusted R2 = 0.825, ß = - 0.908, p < 0.001), corpus uteri (adjusted R2 = 0.554, ß = - 0.746, p < 0.001) and ovarian cancer (adjusted R2 = 0.579, ß = - 0.763, p < 0.001). The higher MIR of gynecologic cancer in LDRs demand for sustainable investment in health systems and balanced cancer control plans in the region.


Assuntos
Colo do Útero/patologia , Neoplasias dos Genitais Femininos/epidemiologia , Neoplasias dos Genitais Femininos/mortalidade , Disparidades em Assistência à Saúde , Desenvolvimento Humano , Fatores Socioeconômicos , Adulto , Distribuição por Idade , Fatores Etários , Feminino , Humanos , Incidência , Expectativa de Vida , Pessoa de Meia-Idade , Mortalidade , Neoplasias Ovarianas/epidemiologia , Características de Residência , Neoplasias do Colo do Útero/epidemiologia , Neoplasias Uterinas/epidemiologia
11.
Environ Sci Pollut Res Int ; 27(10): 10778-10789, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31942718

RESUMO

This study examines the effects of electricity consumption, financial development, economic growth, trade and ICT on CO2 emissions in the fast-emerging countries, excluding Russia due to the unavailability of data. Cross-sectional dependency was identified using the Pesaran (2004) and Breusch and Pagan CD tests from Breusch and Pagan (1980) using annual data from 1993 to 2014 based on data availability. The second-generation panel unit root test was applied to investigate the integration order of the series. The long-run relationship among the variables was confirmed using second-generation panel cointegration techniques, which take cross-sectional dependency into account. Additionally, this study utilized the FMOLS, DOLS and robust least square estimators to determine the long-run coefficients. The results suggested that electricity usage and financial development have a positive and significant impact, while economic growth and trade have a negative and significant impact on CO2 emissions. Additionally, an inverted U-shaped relationship between ICT and CO2 emission was confirmed. This implies that pollution declines after attaining a threshold point as the ICT usage increases. Furthermore, the Dumitrescu and Hurlin (2012) heterogeneous panel causality test suggested that there is a unidirectional causal relationship between electricity consumption and CO2 emissions, CO2 emissions and ICT, gross domestic product and CO2 emissions. Another unidirectional causality exists between financial development and CO2 emissions. The study suggests that renewable energy sources can be adopted to decrease carbon emissions and to promote clean energy. Financial development needs to be further strengthened to promote the use of eco-friendly ICT products.


Assuntos
Dióxido de Carbono/análise , Desenvolvimento Econômico , Estudos Transversais , Produto Interno Bruto , Federação Russa
12.
Saudi J Kidney Dis Transpl ; 30(3): 706-709, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31249237

RESUMO

Diabetes mellitus (DM) is a common disease in Oman as in rest of Gulf Cooperation Council where metabolic syndrome is of high prevalence. DM is a foremost risk factor for urinary tract infections (UTIs). It is also linked to more complicated infections such as emphysematous pyelonephritis (EPN), emphysematous pyelitis (EP), renal/perirenal abscess, emphysematous cystitis, xanthogranulomatous pyelonephritis, and renal papillary necrosis. The diagnosis of these cases is frequently delayed because the clinical manifestations are generic and not different from the typical triad of upper UTI, which include fever, flank pain, and pyuria. A middle-aged female with DM and chronic kidney disease stage IV was admitted with recurrent UTI with extended-spectrum beta-lactamase-producing Escherichia coli. At presentation, she was afebrile, clinically stable, had no flank pain and there was no leukocytosis. Laboratory test for C- reactive protein done twice and was only mildly elevated at 7 and 11 mg/dL. A computed tomography scan of kidney-ureter-bladder (CT-KUB) was recommended and reported as "no KUB stone but small atrophic left kidney with dilatation of the pelvicalycial system and ureter and the presence of air in the collecting system suggestive of EP." Thus, commonly associated with DM, especially in females, debilitated immune-deficient individuals, and patients harboring obstructed urinary system with infective nidus. Air in the kidney is not always due to EPN. UTI with a gas-producing organism can ascend to the kidney in the presence of vesicoureteral reflux.


Assuntos
Complicações do Diabetes/mortalidade , Enfisema/microbiologia , Infecções por Escherichia coli/microbiologia , Pielite/microbiologia , Infecções Urinárias/microbiologia , Refluxo Vesicoureteral/complicações , Doenças Assintomáticas , Complicações do Diabetes/diagnóstico , Enfisema/diagnóstico por imagem , Infecções por Escherichia coli/complicações , Infecções por Escherichia coli/diagnóstico , Feminino , Humanos , Pessoa de Meia-Idade , Pielite/diagnóstico por imagem , Recidiva , Fatores de Risco , Infecções Urinárias/complicações , Infecções Urinárias/diagnóstico , Refluxo Vesicoureteral/diagnóstico
13.
Environ Sci Pollut Res Int ; 25(12): 11536-11555, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29427275

RESUMO

This study investigates the relationship between Internet usage, financial development, economic growth, capital and electricity consumption using quarterly data from 1993Q1 to 2014Q4. The integration order of the series is analysed using the structural break unit root test. The ARDL bounds test for cointegration in addition to the Bayer-Hanck (2013) combined cointegration test is applied to analyse the existence of cointegration among the variables. The study found strong evidence of a long-run relationship between the variables. The long-run results under the ARDL framework confirm the existence of an inverted U-shaped relationship between financial development and electricity consumption, not only in the long-run, but also in the short-run. The study also confirms the existence of a U-shaped relationship between Internet usage and electricity consumption; however, the effect is insignificant. Additionally, the influence of trade, capital and economic growth is examined in both the long run and short run (ARDL-ECM). Finally, the results of asymmetric causality suggest a positive shock in electricity consumption that has a positive causal impact on Internet usage. The authors recommend that the Turkish Government should direct financial institutions to moderate the investment in the ICT sector by advancing credits at lower cost for purchasing energy-efficient technologies. In doing so, the Turkish Government can increase productivity in order to achieve sustainable growth, while simultaneously reducing emissions to improve environmental quality.


Assuntos
Conservação de Recursos Energéticos/economia , Desenvolvimento Econômico/tendências , Eletricidade , Internet/economia , Conservação de Recursos Energéticos/tendências , Desenvolvimento Econômico/estatística & dados numéricos , Internet/tendências , Investimentos em Saúde , Modelos Lineares , Turquia
14.
J Biosoc Sci ; 50(3): 414-420, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-28502253

RESUMO

This study aimed to investigate the frequency of consanguineous marriages and level of understanding of consanguinity-associated genetic risks in the Pashtun population, Pakistan. Information was gathered using a detailed questionnaire completed by 1500 individuals of both sexes over the 11-month period between April 2015 and February 2016. The mean inbreeding coefficient of the respondents was calculated and a five-point response scale was used to assess their understanding of consanguinity risks. The frequency of consanguineous marriages in the Pashtun population was found to be 58.3%, with a mean inbreeding coefficient of 0.0259. Marriage between second cousins was found to be the dominant marriage type. Level of education was found to be negatively related to the incidence of consanguineous marriage (p<0.001), and higher consanguinity was reported among the rural than the urban population (p<0.001). Participants in the ≥25-year age group, those with a higher level of education and those residing in urban areas exhibited a significantly higher understanding of consanguinity risks. The overall prevalence of consanguinity in the Pashtun population is high, demonstrating the need for awareness of its risks in the target population. The timely dissemination of information on potential health-related risks and the introduction of genetic counselling in the region would benefit both the individuals concerned and the community in general.


Assuntos
Consanguinidade , Doenças Genéticas Inatas/psicologia , Conhecimentos, Atitudes e Prática em Saúde , Adulto , Escolaridade , Feminino , Aconselhamento Genético , Doenças Genéticas Inatas/epidemiologia , Doenças Genéticas Inatas/genética , Humanos , Masculino , Prevalência , Estudos Retrospectivos , Risco , População Rural , Inquéritos e Questionários , População Urbana
15.
J Egypt Natl Canc Inst ; 29(3): 123-126, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28669451

RESUMO

BACKGROUND: This study aimed to analyse the correlation between cancer incidence and mortality and the Human Development Index (HDI) in Africa. Furthermore, to analyse the variations in cancer Mortality to Incidence Ratio (MIR) based on health care systems in African countries. MATERIAL AND METHODS: Cancer incidence and mortality data for 53 countries were obtained from GLOBOCAN database. Country-wise data on National-HDI were obtained from Human Development Report 2015. Health System Attainment (HSA) data were acquired from World Health Report 2000. The parametric data were analysed by Pearson-correlation and Linear-regression analysis for the effect of HDI and HSA on MIR in African Countries. One-way ANOVA was used to test the differences of MIR in each HDI group. All analyses were performed in SPSS version 20. RESULTS: An inverse correlation was revealed by cancer MIR with both HDI (r=-0.897, p<0.001) and HSA (r=-0.750, p<0.001). A significantly low MIR was reported from high HDI countries compared to medium and low HDI countries by one-way ANOVA analysis (p<0.001). Linear regression analysis also reported a negative effect of MIR with both HDI (adjusted R2=0 0.801, ß=-0.897, p<0.001) and HSA (adjusted R2=0.554, ß=-0.750, p<0.001). CONCLUSIONS: Based on their HDIs, different African countries has different health system attainments, which is the cause of variations in MIR in these countries. To control their cancer burden, these low and medium HDI countries should focus on improvement of their economic status and policies making, regarding the provision of better health systems to the masses.


Assuntos
Atenção à Saúde , Desenvolvimento Humano , Neoplasias/epidemiologia , África/epidemiologia , Bases de Dados Factuais , Humanos , Incidência , Mortalidade , Neoplasias/mortalidade , Vigilância da População
16.
Turk J Med Sci ; 47(2): 523-530, 2017 Apr 18.
Artigo em Inglês | MEDLINE | ID: mdl-28425241

RESUMO

BACKGROUND/AIM: This study analyzed the incidence, trends, and common types of cancer in the Turkish Republic of Northern Cyprus (TRNC). MATERIALS AND METHODS: This study is based on data collected from the office of the North Cyprus Cancer Registry, Ministry of Health, for 2007-2012. Data were arranged on the basis of age group, sex, and cancer site. Age standardized incidence rates (ASRs) were estimated with the world standard population. EVIEWS (version 9) software was used for statistical analysis. RESULTS: Of 1395 registered cases, 52.33% (730) were reported in men and 47.67% (665) in women. The crude incidence rate was 96.41 in men and 101.74 in women. The average annual ASR was 88.88 in men and 87.76 in women with the cumulative rate of 21.47% and 14.69% in men and women, respectively. The most common cancers in men were skin (ASR 15.62), prostate (ASR 11.23), bladder (ASR 11.71), lung (ASR 8.01), and colorectal cancer (ASR 7.61), while in women these were breast (ASR 24.07), thyroid (ASR 14.93), skin (ASR 10.75), colorectal (ASR 6.05), and lymphoma (ASR 4.79). Linear regression analysis confirmed rising trends for both men's (10.79, P ≤ 0.03) and women's (14.67, P ≤ 0.04) cancers. CONCLUSION: Our findings revealed an increasing trend of cancer incidence in the TRNC. For control and prevention, public awareness of the risk factors and proper screening programs should be recommended.


Assuntos
Neoplasias/epidemiologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , Chipre/epidemiologia , Feminino , Humanos , Incidência , Lactente , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Turquia/epidemiologia , Adulto Jovem
17.
Vet World ; 8(7): 875-80, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-27047168

RESUMO

AIM: Molecular identification and genetic diversity of open reading frame 7 (ORF7) of field isolated porcine reproductive and respiratory syndrome virus (PRRSV) in North Sumatera, Indonesia, in the period of 2008-2014. MATERIALS AND METHODS: A total of 47 PRRSV samples were collected from the death case of pigs. The samples were collected from different districts in the period of 2008-2014 from North Sumatera province. Two pairs of primer were designed to amplify ORF7 of Type 1 and 2 PRRSV based on the sequence of reference viruses VR2332 and Lelystad. Viral RNAs were extracted from samples using PureLink™ micro-to-Midi total RNA purification system (Invitrogen). To amplify the ORF7 of PRRSV, the synthesis cDNA and DNA amplification were performed by reverse transcription polymerase chain reaction (RT-PCR) and nested PCR method. Then the DNA sequencing of PCR products and phylogenetic analysis were accomplished by molecular evolutionary genetics analysis version 6.0 software program. RESULTS: RT-: PCR and nested PCR used in this study had successfully detected of 18 samples positive PRRS virus with the amplification products at 703bp and 508bp, respectively. Sequencing of the ORF7 shows that 18 PRRS viruses isolated from North Sumatera belonged to North American (NA). JXA1 Like and classic NA type viruses. Several mutations were detected, particularly in the area of nuclear localization signal (NLS1) and in NLS2. In the local viruses, which were related closed to JXA1 virus; there are two differences in amino acids in position 12 and 43 of ORF7. Our tested viruses showed that the amino acid positions 12 and 43 are Asparagine and Arginine, while the reference virus (VR2332, Lelystad, and JXA1) occupied both by Lysine. Based on differences in two amino acids at position 12 and 43 showed that viruses from North Sumatera has its own uniqueness and related closed to highly pathogenic PRRS (HP-PRRS) virus (JXA1). CONCLUSION: The results demonstrated that North Sumatera type PRRS virus has caused PRRS outbreaks in pig in North Sumatera between 2008 and 2014. The JAX1 like viruses had unique amino acid residue in position 12 and 43 of asparagine and lysine, and these were genetic determinants of North Sumatera viruses compared to other PRRS viruses.

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