A customized multi-cycle model for measuring the sustainability of circular pathways in agri-food supply chains.

Circular economy (CE) is claimed to be a promising pathway to achieve the Sustainable Development Goals (SDGs), but a reliable metric is needed to validate closed-loop strategies by measuring sustainability performances together with the degree of circularity. A significant contribution is offered by Life Cycle (LC) scholars in terms of methodological advances and operational tools for different sectors, also those more complex such as the agro-industrial systems that encompass biological and anthropogenic variables at different scales. However, to date, LC methodologies have not yet answered how to model the complexity of circular pathways. LC evaluations are often modelled for cradle-to-grave analyses, while a circularity evaluation would require an extension of the system boundaries to more interconnected life cycles, orienting towards a cradle-to-cradle perspective. This research gap led us to propose a multi-cycle approach with expanded assessment boundaries, including co-products, into a cradle-to-cradle perspective, in an attempt to internalize circularity impacts. The customized LC framework here proposed is based on the Life Cycle Assessment (LCA), the Environmental Life Cycle Costing (ELCC) in terms of internal and external costs, and the Social Life Cycle Assessment (SLCA) in terms of Psychosocial Risk Factor (PRF) impact pathway. The model is designed to be applied to the olive-oil sector, which commonly causes significant impacts by generating many by-products whose management is often problematic. Results are expected to show that the customized LC framework proposed can better highlight the environmental and socioeconomic performances of the system of cycles, allowing CE to deliver its promises of sustainability, as the circularity of materials per se is a means, not an end in itself.

Increasing the Content of Olive Mill Wastewater in Biogas Reactors for a Sustainable Recovery: Methane Productivity and Life Cycle Analyses of the Process.

Anaerobic codigestion of olive mill wastewater for renewable energy production constitutes a promising process to overcome management and environmental issues due to their conventional disposal. The present study aims at assessing biogas and biomethane production from olive mill wastewater by performing biochemical methane potential tests. Hence, mixtures containing 0% (blank), 20% and 30% olive mill wastewater, in volume, were experimented on under mesophilic conditions. In addition, life cycle assessment and life cycle costing were performed for sustainability analysis. Particularly, life cycle assessment allowed assessing the potential environmental impact resulting from the tested process, while life cycle costing in conjunction with specific economic indicators allowed performing the economic feasibility analysis. The research highlighted reliable outcomes: higher amounts of biogas (80.22 ± 24.49 NL.kgSV-1) and methane (47.68 ± 17.55 NL.kgSV-1) were obtained when implementing a higher amount of olive mill wastewater (30%) (v/v) in the batch reactors. According to life cycle assessment, the biogas ecoprofile was better when using 20% (v/v) olive mill wastewater. Similarly, the economic results demonstrated the profitability of the process, with better performances when using 20% (v/v) olive mill wastewater. These findings confirm the advantages from using farm and food industry by-products for the production of renewable energy as well as organic fertilizers, which could be used in situ to enhance farm sustainability.

Highly efficient and automated extraction of DNA from human remains using a modified EZ1 protocol.

Bones and teeth often represent the only sources of DNA available for identifying human remains. DNA in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of calcium. Because of the extensive mineralisation, the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction (PCR) inhibitors. Some protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification platform. To improve the efficiency of DNA extraction from skeletal remains, the present study focuses on a modification to these already available protocols. In this study, different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol, a supplementary protocol, and a modified protocol. The modified approach included a decalcification step, whereas the Qiagen protocols worked directly on non-decalcified powder. In all three procedures, 150 mg samples were used for DNA extraction. We evaluated the quantity of DNA recovered from samples, the presence of any PCR inhibitors co-extracted, the level of DNA degradation, the quality of short tandem repeat (STR) profiles, and the reproducibility of the modified procedure. When compared with the other protocols, the modified protocol resulted in the best recovery of DNA that was free of PCR inhibitors. Additionally, the STR profiles were reliable and of high quality. In our opinion, the decalcification step increases DNA recovery by softening tissues, which allows lysis solutions to act more effectively. Furthermore, the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based protocols. These findings may be helpful solutions to the problems commonly encountered when dealing with difficult samples, such as bones and teeth.Key pointsBones and teeth often represent the only sources of DNA for identifying human remains.The choice of an efficient DNA extraction procedure is important for maximizing DNA recovery and removing PCR inhibitors.This study focuses on modifications to the previously available Qiagen-based protocols.The modified protocol enabled the best recovery of DNA, and both quality and quantity were superior to those of the previously available Qiagen-based protocols.The STR profiles obtained from samples extracted using the modified protocol were reliable and of high quality.

Harvesting system sustainability in Mediterranean olive cultivation: Other principal cultivar.

In the olive production sector, which is increasingly expanding beyond the borders of the Mediterranean basin, harvesting is the most demanding phase, from both an economic and organisational point of view. Traditional olive orchards are still predominant, with centuries-old and large plants, and are characterised by the gradual ripening of drupes and irregular planting patterns. Even though the structural conversion of these olive orchards into more modern cultivations may be difficult owing to their historical, monumental, and landscaping importance, as well as the existing legal restrictions, supporting a "modernisation" process aimed at mechanising the main farming operations remains a priority. Technological innovation is, therefore, a primary objective for Mediterranean olive growing, as well as for the enhancement of its strengths. The present study aimed at assessing different olive harvesting sites, considering the technical, economic, and environmental aspects, to develop a better version of the "olive harvesting database". The applied methodology, also called the "modular approach", represents a useful tool to apply in unitary process assessment to obtain a comprehensive database of diverse agricultural operations. Eight olive harvesting systems were compared: six highly mechanised scenarios, one based on mechanical-aided harvesting, and the final one based on fully manual harvesting. The mechanised systems obtained a better performance in terms of working capacity, as only 3.5 h ha-1 were needed to harvest 12 t using a self-propelled trunk shaker. In addition, the economic results revealed that mechanical harvesting, diversely from manual or aided harvesting, is the only way to decrease production costs. From an environmental point of view, manual and mechanical-aided harvesting showed the best performance in terms of impact per hour. However, using the mass-based unit (1 kg of harvested olives), the results were the opposite and this could be very relevant for the ecoprofile of olive oil.

A Life Cycle Perspective to Assess the Environmental and Economic Impacts of Innovative Technologies in Extra Virgin Olive Oil Extraction.

Advances in the adoption of technological innovations represent a great driver to improve the competitiveness of the Italian extra virgin olive oil (EVOO) industry. This work assesses the efficiency of an innovative extraction plant (with low oxidative impact, heating of paste before malaxation and a special decanter that avoids the final vertical centrifugation) in terms of oil yield and quality, and economic and environmental impacts. Economic and environmental impacts were evaluated by using both life cycle costing and life cycle assessment methodologies. A sensitivity analysis was also performed to highlight the uncertain factors that may strongly affect the results. Findings showed that olive milling with the innovative plant resulted in olive oil with a significant increase in quality, although the extraction yield was significantly higher when using conventional technology. In terms of environmental results, an average growth of 4.5% of the impacts in all categories was reached. The economic results revealed the highest extraction cost for the innovative scenario as well as the lower profitability, although a positive return in investment feasibility can be achieved due to an increase in the olive oil selling price. These findings could be useful to highlight the main hotspots in EVOO production and to suggest improvements for more sustainable management.

Life cycle assessment of olive oil: A case study in southern Italy.

The paper describes the results of a specific LCA based analysis of the production of olive oil in the region of Calabria, in southern Italy. The goal of the study is to assess the energy and environmental impacts of different scenarios involving conventional and organic cultivations, plains and hills cultivations and involving different operating techniques. The study also aims at assessing the share of each life cycle step on the total of energy and environmental impacts. The functional unit chosen for the comparative analysis is a glass bottle of 0.75 L of extra virgin olive oil. A "from cradle to gate" perspective was chosen. The analysis was developed according to the LCA standards of the ISO 14040 series. The analysis is based on a field analysis developed in the last years in the province of Reggio Calabria between more than 50 enterprises and stakeholders of the field, representative of the whole Calabria region and of most southern Italy. The data used for the development of mass and energy balances are related to the years 2013-2015. The results clarify that for all indicators that the first part of the life cycle - from the production, including the growth of the olive plant to the full production stage - is the most relevant, variable between 80.6% share in the case of the particulate matter indicator to the 99.64% in the case of land use (Hill - Biological agriculture scenario). Relevant differences can be also traced for each specific indicator among all scenarios; high impacts are traced for the agricultural stages among all scenarios (70% -90% in all indicators) with high impacts caused by fertilizers. Among the transformation stages the bottle production is one of the most relevant sources of life cycle energy uses and environmental impacts (80-90%).

Harvesting system sustainability in Mediterranean olive cultivation.

The mechanization of farming operation plays an important role in improving the profitability of the agricultural sector by increasing work productivity and reducing production costs. However, the new challenges of agriculture also include the environmental issues. The choice between different alternatives to perform a determined agricultural practice should be based on reliable information, considering technical, economic and environmental aspects. Olive growing represents the most important agricultural production in the Mediterranean Basin and its mechanization, particularly harvesting, could have major impacts on the sustainability of this production. This study aims at assessing various olive-harvesting scenarios, while considering technical, economic and environmental aspects in order to build a beta version of the "olive-harvesting database". The proposed methodology called "modular approach" could represent a useful tool to apply in unitary process assessment in order to obtain a comprehensive database of the diverse agricultural operations. The methodology was based on Life Cycle Assessment and production cost analysis. Technical performance evaluation showed that the recorded work capacities varied between 5 tons of harvested olives per day when employing mechanical harvest aids and 18 tons per day when employing trunk shakers. The economic evaluation highlighted that the harvesting costs are variable as a function of the given cost type (costs per hour, costs per kg of harvested olives and costs per hectare). The LCA revealed that mechanically aided techniques were the most sustainable ones when the functional unit is considered as one harvesting hour, although this FU is not the most suitable unit for choosing the best environmental solution. The surface and production mass units are more appropriate FUs in comparative studies, although they are strictly linked to the "work capacity". A significant variation in the environmental performances depended on the FUs and on the average yields when the FU represented one kg of harvested olives.

Shelf life extension as solution for environmental impact mitigation: A case study for bakery products.

Over the last years, increasing attention has been paid to environmental concerns related to food production and potential solutions to this issue. Among the different strategies being considered to reduce the impact food production has on the environment, only moderate has been paid to the extension of shelf life; a longer shelf life can reduce food losses as well as the economic and environmental impacts of the distribution logistics. The aim of this study is to assess the environmental performance of whole-wheat breadsticks with extended shelf lives and to evaluate whether the shelf-life extension is an effective mitigation solution from an environmental point of view. To this purpose, the life cycle assessment (LCA) approach was applied from a "cradle-to-grave" perspective. Rosmarinic acid was used as an antioxidant to extend the shelf life. To test the robustness of the results and to investigate the influence of the choices made in the modelling phase, a sensitivity and uncertainty analysis were carried out. The achieved results highlighted how, for 10 of the 12 evaluated impact categories, the shelf-life extension is a proper mitigation solution, and its effectiveness depends on the magnitude of product loss reduction that is achieved. The shelf-life extension doesn't allow for the reduction of environmental impact in the categories of human toxicity, cancer effects and freshwater eutrophication.

Life cycle tools combined with multi-criteria and participatory methods for agricultural sustainability: Insights from a systematic and critical review.

Life cycle (LC) methodologies have attracted a great interest in agricultural sustainability assessments, even if, at the same time, they have sometimes been criticized for making unrealistic assumptions and subjective choices. To cope with these weaknesses, Multi-Criteria Decision Analysis (MCDA) and/or participatory methods can be used to balance and integrate different sustainability dimensions. The purpose of this study is to highlight how life cycle approaches were combined with MCDA and participatory methods to address agricultural sustainability in the published scientific literature. A systematic and critical review was developed, highlighting the following features: which multi-criterial and/or participatory methods have been associated with LC tools; how they have been integrated or complemented (methodological relationships); the intensity of the involvement of stakeholders (degree of participation); and which synergies have been achieved by combining the methods. The main typology of integration was represented by multi-criterial frameworks integrating LC evaluations. LC tools can provide MCDA studies with local and global information on how to reduce negative impacts and avoid burden shifts, while MCDA methods can help LC practitioners deal with subjective assumptions in an objective way, to take into consideration actors' values and to overcome trade-offs among the different dimensions of sustainability. Considerations concerning the further development of Life Cycle Sustainability Assessment (LCSA) have been identified as well.

Social life cycle assessment and participatory approaches: A methodological proposal applied to citrus farming in Southern Italy.

UNLABELLED: Recently, Social Life Cycle Assessment (S-LCA) has been developed under the methodological framework of Life Cycle Thinking (LCT) to evaluate the social impacts that emerge during the overall life cycle of a product or service. There is not yet a standardized methodology for S-LCA as there is for environmental LCA (eLCA), due to the nature of social impacts that do not depend only on the processes themselves, but also on the behavior and context of actors (manufactures, consumers, local community members, etc.). One of the most critical steps in the application of S-LCA concerns the choice of criteria for selecting affected actors, impact categories, subcategories, and the taxonomic relation among them. Moreover, the importance (in terms of weight) of these impacts may be felt differently by affected actors, confirming the importance of the context within which impacts arise. In this sense, the integration of participatory tools can be useful in making the S-LCA more locally relevant. The aim of the present study is twofold. First, we will outline a methodology that combines S-LCA with two research tools. The first is the focus group, adopted from qualitative research. The second is the Analytic Hierarchy Process (AHP), adopted from operational research, which belongs to the framework of Multicriteria Decision Analysis (MCDA). These have been used to make the S-LCA more locally relevant and to legitimate the criteria used. Second, we will test this methodology by applying it to a specific field, i.e., 3 production areas and 3 different crop systems of citrus growing in the Calabria region in Southern Italy. Citrus growing is one of the most important agricultural sectors at regional level, and it is also well known for issues of social concern, particularly in relation to immigrant workers. The results show a number of differences between cases and could offer useful insights to both local decision makers, such as agricultural entrepreneurs, and to those public decision makers that design and implement territorial planning strategies. Results have allowed the authors to rank the social performance of each case and to reflect on the most critical steps in conducting an S-LCA. KEY FINDINGS: The integration of qualitative techniques and a multicriteria in sLCA allows catching local specificities by involving local experts and stakeholders Results highlighted that impact categories mostly contributed to performance differences Public deciders can be supported in deciding which farming practices should be encouraged, which social domains must be paid more attention, and where social problems mostly occur The methodological application allowed the authors also to foresee the feasibility of the integration of LCA and LCC results as inputs in sLCA to conduct a Life Cycle Sustainability Assessment (LCSA).

Genetic study of 11 Y-STRs in the populations of Reggio Calabria, Catanzaro, Cosenza (Calabria--South of Italy).

The ability to identify male-specific DNA renders Y-chromosomal STR systems an invaluable help in cases of rape, other sexual assault as well as in kinship testing. The "PowerPlex Y System" is a recent kit by Promega, that co-amplifies the repeat regions of 11 Y-STRs loci. In the present study, we analyzed the allelic distribution of Y-STRs loci in around 300 unrelated males belonging to the three populations of Calabria region.