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Mol Gen Mikrobiol Virusol ; (2): 33-6, 1995.
Artigo em Russo | MEDLINE | ID: mdl-7477032

RESUMO

The cDNA fragments complementary to RNA-polymerase gene and 3'-untranslated genome region of attenuated foot-and-mouth disease virus strain A(22)645 have been synthesized and cloned into a plasmid vector pUC19 in E. coli JM109. The cloned cDNA fragments were characterized as to their size, orientation towards the plasmid, and localization in the virus genome. Restriction maps for complete gene and two cDNA clones were constructed.


Assuntos
Aphthovirus/genética , RNA Polimerases Dirigidas por DNA/genética , Fragmentos de Peptídeos/genética , Aphthovirus/enzimologia , Aphthovirus/imunologia , Clonagem Molecular , DNA Complementar , RNA Polimerases Dirigidas por DNA/química , Escherichia coli/genética , Plasmídeos , Vacinas Atenuadas/genética , Vacinas Virais/genética
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