Transport and retention of graphene oxide nanoparticles in sandy and carbonaceous aquifer sediments: Effect of physicochemical factors and natural biofilm.

There is a paucity of information regarding the interaction between GONPs and natural aquifer sediments. Therefore, batch and column experiments were carried out to determine the transport, retention and attachment behavior of GONPs with the surfaces of native aquifer sediments. The experiments were performed with sediments comprising contrasting mineralogical features (sand grains, quartz and limestone sediments), at different temperatures, ionic strength and compositions. Uniquely, this research also investigated the effect of natural biofilm on the retention behavior of nanoparticles in porous media. The retention rate of GONPs at 22 °C was higher than at 4 °C. Moreover, there was greater retention of GONPs onto the surfaces of collectors at higher ionic strengths and cation valence. The retention profiles (RPs) of GONPs in pristine porous media at low ionic strength were linear, which contrasted with hyper-exponential shape of RPs at high ionic strength. The size-distribution analysis of retained GONPs showed decreasing particle diameter with increasing distance from the column inlet at high ionic strength and equal diameter at low ionic strengths. The GONP retention rate was higher for natural porous media than for sand, due to the presence of metal oxides heterogeneities. The presence of biofilm on porous media increased the retention rate of GONPs when compared to the porous media in the absence of biofilm.

Changes of viral and prokaryote abundances in a high rate algal pond using flow cytometry detection.

High rate algal ponds (HRAPs) are shallow, mixed systems for wastewater treatment, which use sunlight exposure for disinfection. Little is known regarding the relationships between the bacteria and viruses within HRAP systems. Uniquely, flow cytometry permits the rapid identification of bacterial and viral populations in wastewater samples, separating populations based on genome and particle size. Treated wastewater samples were collected from an HRAP at Kingston on Murray, South Australia. Flow cytometry analysis detected bacterial populations and discriminated virus-like particles (VLP) and large VLP (LVLP). Rapid, short term, fluctuations in the abundance of all three populations were observed. Changes in the abundance of these populations was compared; wastewater composition was used as metadata for the comparisons. Linear regression determined relationships in abundances between bacteria and LVLP (R2 0.2985); LVLP and VLP (R2 0.5829) and bacteria and VLP (R2 0.5778) all with p-values of <0.001. Bacterial, LVLP and VLP abundance positively correlated with each other, indicating potential microbial interactions. Overall, the results suggest a parasitic relationship was occurring and driving the abundances of bacteria and viruses within the system.

Role of biofilm on virus inactivation in limestone aquifers: implications for managed aquifer recharge.

BACKGROUND: Virus, as nano-sized microorganisms are prevalent in aquifers, which threaten groundwater quality and human health wellbeing. Virus inactivation by attachment onto the limestone surfaces is a determining factor in the transport and retention behavior of virus in carbonaceous aquifers. METHODS: In the present study, the inactivation of MS2 -as a model virus- by attachment onto the surfaces of limestone grains was investigated in a series of batch experiments under different conditions such as limestone particle size distribution (0.25-0.50, 0.5-1 and 1-2 mm), treated wastewater and RO water, temperature (4 and 22 °C), initial MS2 concentrations (103-107 PFU/mL) and static and dynamic conditions. The experimental data of MS2 inactivation was also fitted to a non-linear kinetic model with shoulder and tailing. The characteristics of biofilm on the surfaces of limestone aquifer materials were assessed using scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). RESULTS: The inactivation rate of virus decreased with increasing the adsorbent diameter. Furthermore, virus inactivation was greater at room temperature (22 °C) than 4 °C, in both static and dynamic conditions. The inactivation of virus via attachment onto the limestone aquifer materials in dynamic conditions was higher than under static conditions. In addition, fitting the experimental data with a kinetic model showed that virus inactivation was high at higher temperature, smaller limestone grains and dynamic conditions. Moreover, the experiments with treated wastewater showed that in authentic aqueous media, the virus inactivation was considerably higher than in RO water, due to the presence of either monovalent or divalent cations and surface roughness created by biofilms. CONCLUSION: Finally, in terms of managed aquifer recharge systems, the presence of biofilm increases bacteria and virus retention onto the aquifer surfaces. Graphical abstract.

Effect of bacteria and virus on transport and retention of graphene oxide nanoparticles in natural limestone sediments.

This research was conducted to evaluate the effect of co-transport of different-sized microorganisms on graphene oxide nanoparticles (GONPs) transport and retention in saturated pristine and biofilm-conditioned limestone columns. The transport and retention behavior of GONPs was studied in columns in the presence of MS2 -as a nano-sized- and Escherichia coli (E.coli) -as a micro-sized- microorganisms at low and high ionic strength conditions. Results showed no changes in GONPs transport and retention at high ionic strength in the presence of MS2 or E. coli, which was attributed to the effect of high concentration of divalent cation on aggregation of nanoparticles and microorganisms. Furthermore, simultaneous enhanced transport and decreased retention of GONPs in column was observed in the co-presence of microorganisms at low ionic strength. Results revealed that the main mechanism governing increasing GONPs transport in porous media was occupation of reactive surface sites of collectors by microorganisms, which prevented attachment of nanoparticles. The pre-saturation of columns with MS2 and E. coli caused increasing transport of GONPs in the columns, due to the occupation of surface reactive sites. Moreover, conditioning limestone collectors with natural biofilm resulted in the same rates of nanoparticle elution and retention (i.e., in the presence or absence of microorganisms) by straining of GONPs in the inlet end of columns which shows that the biofilm acts as a bio-filter against discharging nanoparticles into the effluents. Finally, from the obtained results, it can be postulated that the presence of microorganisms in a MAR site causes risk of groundwater pollution by toxic nanoparticles.

Case study on the effect continuous CO2 enrichment, via biogas scrubbing, has on biomass production and wastewater treatment in a high rate algal pond.

Microalgae grown in high rate algal ponds (HRAP) treating wastewater are considered a promising feed for biofuel production. Biomass productivity is often considered to be limited by carbon availability, with the addition of CO2 being the proposed solution. Biogas from anaerobic wastewater treatment potentially provides a cheap, co-located CO2 source. Two identical 223 m2 HRAPs were constructed at Melbourne Water's Western Treatment Plant, where biogas from an anaerobic lagoon is used to generate electricity. One HRAP was fed secondary treated wastewater that had been enriched with CO2 recovered from the biogas using industry standard biogas scrubbers, the Enriched HRAP, while the other HRAP was fed the same wastewater expect it had by passed the biogas scrubbers, the Control HRAP. The biomass production and wastewater treatment performance of the two HRAPs was compared over 12 months. The inlet to the Enriched HRAP had significantly higher free CO2 and inorganic carbon, 175.00 ±â€¯49.30 mg L-1 and 110.00 ±â€¯10.2 mg L-1, than the inlet to the Control HRAP, 9.30 ±â€¯7.08 mg L-1 and 89.62 ±â€¯5.12 mg L-1. There were no significant differences in biomass production between the HRAPs as measured by dry matter, particulate organic carbon or nitrogen. Chlorophyll a was statistically higher in the Enriched HRAP, however, this measurement is potentially unreliable. Regarding wastewater treatment, only total nitrogen and ammonium removal differed significantly between the HRAPs, with the Control HRAP, 59.13 ±â€¯21.13% and 76.46 ±â€¯32.33%, slightly outperforming the Enriched HRAP, 53.52 ±â€¯17.41% and 68.76 ±â€¯31.17%. Overall, neither biomass production nor wastewater treatment was meaningfully improved by CO2 enrichment, however, wastewater treatment was still effective in both HRAPs.

Nitrification performance of high rate nitrifying trickling filters at low ammonia concentrations: does the aspect ratio matter?

Nitrifying trickling filters (NTFs) are often introduced to pre-treat waters before chlorination process, to reduce the ammonia-driven chlorine consumption in wastewater treatment. As a passive aerated system, the only power needed is to transport the water to the top of the filter for distribution. Thus, understanding the role of filter aspect ratio on ammonia oxidation might save energy cost. In the present study, a pilot-scale comparison NTF system was conducted on two filters with different aspect ratios (height/diameter) and the same specific surface area. The nitrification efficiencies of these two filters under relatively low influent ammonia-nitrogen concentrations (1.0-4.0 mg NH4-N L-1) were investigated. Results obtained from the present study indicated that the constructional aspect ratio of NTF showed no significant effect on nitrification performance of NTFs. Additionally, the operational parameters showed similar effects on nitrification in NTFs with different aspect ratios. Our findings could provide important information for the construction design of future NTFs.

Comparison of the treatment performance of a high rate algal pond and a facultative waste stabilisation pond operating in rural South Australia.

South Australian community wastewater management schemes (CWMS) treat wastewater using waste stabilisation ponds before disposal or reuse. This study compared the performance of a facultative pond, 6,300 m2, 27.5 d theoretical hydraulic retention time (THRT), with a high rate algal pond (HRAP) operated at depths of 0.32, 0.43 and 0.55 m with THRT equivalent to 4.5, 6.4 and 9.1 d respectively. Both ponds received influents of identical quality, differing only in quantity, and were operated in similar climatic conditions. The depth of HRAP operation had only a minor influence on treatment performance. The study showed that the quality of the treated effluent from the HRAP was equivalent to that of the facultative pond, 5-day biochemical oxygen demand removal >89%, NH4-N removal 59.09-74.45%. Significantly, Escherichia coli log10 reduction values by the HRAP, 1.74-2.10, were equivalent to those of the facultative pond. Consequently, HRAPs could replace facultative ponds within CWMS while maintaining treated effluent quality. The benefit would be halving the surface area requirement from 4.2 m2 capita-1 for the facultative pond to between 2.0 and 2.3 m2 capita-1, depth dependent, for an HRAP, with significant attendant reductions in the capital costs for construction.

Natural and surfactant modified zeolites: A review of their applications for water remediation with a focus on surfactant desorption and toxicity towards microorganisms.

The objective of this review is to highlight the need for further investigation of microbial toxicity caused by desorption of surfactant from Surfactant Modified Zeolite (SMZ). SMZ is a low cost, versatile permeable reactive media which has the potential to treat multiple classes of contaminants. With this combination of characteristics, SMZ has significant potential to enhance water and wastewater treatment processes. Surfactant desorption has been identified as a potential issue for the ongoing usability of SMZ. Few studies have investigated the toxicity of surfactants used in zeolite modification towards microorganisms and fewer have drawn linkages between surfactant desorption and surfactant toxicity. This review provides an overview of natural zeolite chemistry, characteristics and practical applications. The chemistry of commonly used surfactants is outlined, along with the kinetics that drive their adsorption to the zeolite surface. Methodologies to characterise this surfactant loading are also described. Applications of SMZ in water remediation are highlighted, giving focus to applications which deal with biological pollutants and where microorganisms play a role in the remediation process. Studies that have identified surfactant desorption from SMZ are outlined. Finally, the toxicity of a commonly used cationic surfactant towards microorganisms is discussed. This review highlights the potential for surfactant to desorb from the zeolite surface and the need for further research into the toxicity of this desorbed surfactant towards microorganisms, including pathogens and environmental microbes.

The toxicity of cationic surfactant HDTMA-Br, desorbed from surfactant modified zeolite, towards faecal indicator and environmental microorganisms.

Surfactant Modified Zeolite (SMZ) represents a versatile, cost-effective permeable reactive material, capable of treating multiple classes of contaminants. The potential for HDTMA-Br, a cationic surfactant commonly used to modify zeolite, to desorb from the zeolite surface has been identified as a potential issue for the ongoing use of SMZ in water remediation contexts. This paper investigates the toxicity of HDTMA-Br towards enteric virus surrogates, F-RNA bacteriophage MS2 and E. coli, Bacillus subtilis, and soil microflora. The concentration of surfactant desorbing from SMZ was quantified through a bioassay using E. coli. Results showed HDTMA-Br concentrations of ≥10-5M were toxic to MS2, ≥10-4M were toxic to E. coli and ≥10-6M were toxic to B. subtilis. No toxic relationship was established between HDTMA-Br and soil microflora. Desorption of ≥10-4M of HDTMA-Br was shown for the two SMZ samples under the mixing conditions used. Effects of this surfactant on total soil microflora were ambiguous since no toxic relationship could be established, however, HDTMA-Br, at concentrations desorbing from SMZ, were shown to impact the soil bacterium B. subtilis. Further research is required to determine the effect of this surfactant on microbial populations and species diversity in soils.

Structure of nitrifying biofilms in a high-rate trickling filter designed for potable water pre-treatment.

This study examined the composition and structure of nitrifying biofilms sampled from a high-rate nitrifying trickling filter which was designed to pre-treat raw surface water for potable supply. The filter was operated under a range of feed water ammonia and organic carbon concentrations that mimicked the raw water quality of poorly protected catchments. The biofilm structure was examined using a combination of fluorescence in situ hybridisation and scanning electron microscopy. Biopolymers (carbohydrate and protein) were also measured. When the filter was operated under low organic loads, nitrifiers were abundant, representing the majority of microorganisms present. Uniquely, the study identified not only Nitrospira but also the less common Nitrobacter. Small increases in organic carbon promoted the rapid growth of filamentous heterotrophs, as well as the production of large amounts of polysaccharide. Stratification of nitrifiers and heterotrophs, and high polysaccharide were observed at all filter bed depths, which coincided with the impediment of nitrification throughout most of the filter bed. Observations presented here specifically linked biofilm structure with filter functionality, physically validating previous empirical modelling hypotheses regarding competitive interactions between autotrophic and heterotrophic bacteria in biofilms.

The adsorption of cyanobacterial hepatoxins as a function of soil properties.

Cyanobacterial hepatotoxins present a risk to public health when present in drinking water supplies. Existing removal strategies, although efficient, are not economically viable or practical for remote Australian communities and developing nations. Bank filtration is a natural process and a potential low cost, toxin removal strategy. Batch studies were conducted in 12 texturally diverse soils to examine the soil properties influencing the adsorption of the cyanobacterial hepatotoxins, microcystin-LR and nodularin. Sorption isotherms were measured. Freundlich and linear isotherms were observed for both toxins with adsorption coefficients not exceeding 2.751 kg(-1) for nodularin and 3.81 kg(-1) for microcystin. Significant positive correlations were identified between hepatotoxin sorption and clay and silt contents of the soils. Desorption of toxins was also measured in three different soils. Pure nodularin and microcystin-LR readily desorbed from all soils.