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1.
Int J Biol Macromol ; 203: 583-592, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35090942

RESUMO

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.


Assuntos
Melanoma , Espectrometria de Massas em Tandem , Animais , Melanoma/metabolismo , Proteínas , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Suínos , Porco Miniatura
2.
Food Chem ; 368: 130499, 2022 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-34496333

RESUMO

Here, we present the potential analytical applications of photochemistry in combination with fluorescence fingerprinting. Our approach analyzes the fluorescence of samples after ultraviolet light (UV) treatment. Especially in presence of metal ions and thiol-containing compounds, the fluorescence behavior changes considerably. The UV-induced reactions (changes) are unique to a given sample composition, resulting in distinct patterns or fingerprints (typically in the 230-600 nm spectral region). This method works without the need for additional chemicals or fluorescent probes, only suitable diluent must be used. The proposed method (UV fingerprinting) suggests the option of recognizing various types of pharmaceuticals, beverages (juices and wines), and other samples within only a few minutes. In some studied samples (e.g. pharmaceuticals), significant changes in fluorescence characteristics (mainly fluorescence intensity) were observed. We believe that the fingerprinting technique can provide an innovative solution for analytical detection.


Assuntos
Corantes Fluorescentes , Raios Ultravioleta , Íons , Metais , Análise Espectral
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