The role of the ABF1 gene in regulation of Cd-induced hormesis in Arabidopsis thaliana.

Hormesis is important in plant performance in contaminated environments, but the underlying genetic mechanisms are poorly understood. This study aimed at mining key genes in regulating Cd-induced hormesis in Arabidopsis thaliana and verifying their biological function. Hormesis of fresh weight, dry weight, and root length occurred at concentrations of 0.003-2.4, 0.03-0.6, and 0.03-0.6 µM Cd, respectively. Superoxide dismutase and catalase activities, and chlorophyll content displayed inverted U-shaped curves, indicating that the antioxidant defense system and photosynthesis system played roles in hormesis. Based on KEGG pathway analysis with the trend chart of differentially expressed genes and weighted correlation network analysis, the key gene ABF1 in the metabolic pathway of abscisic acid was identified. Subsequently, genetic experiments with wild, overexpressing, and knockdown lines of A. thaliana were conducted to further verify the biological function of ABF1 involving Cd-induced hormesis in A. thaliana. The results revealed that the resistance capability of the overexpressing type to Cd stress was significantly enhanced and implicated that the ABF1 gene is essential for Cd-induced hormesis in A. thaliana. Mining key genes that regulate Cd-induced hormesis in plants and stimulate them could have a transformative impact on the phytoremediation of metal-contaminated environments.

New insights into the role of soil properties in driving cadmium-induced hormesis in soil alkaline phosphatase under vegetation cover change.

This study investigated the hormetic responses of soil alkaline phosphatase (ALP) to exogenous Cd under five different vegetation cover types in a typical coastal wetland, including mudflat (Mud), Phragmites australis (PA), Spartina alterniflora (SA), Metasequoia glyptostroboides (MG), and Cinnamomum camphora (CC). The results showed that the activity of soil ALP was significantly enhanced by exogenous 0.3-1.0, 0.2-0.8, 0.05-0.3, 0.05-0.6, and 0.05-0.60 mg Cd /kg in Mud, PA, SA, MG, and CC, respectively. Moreover, the Horzone (an integrated indicator of the stimulation phase) of Mud and PA was significantly higher than that of SA, MG, and CC. Multiple factor analysis revealed that soil chemical properties and soil bacteria community play an important role in the hormetic effect of soil ALP to Cd stress. Soil electric conductivity (EC) and the relative abundance of Gammaproteobacteria were also identified as key drivers of the hormetic effects of Cd on soil ALP under five vegetation cover types. These findings suggest that the soil ecosystem had better resistance to exogenous Cd stress under mudflat and native species (PA) than invasive species (SA), and artificial forests (MG and CC) when soil ALP activity was the test endpoint. Consequently, this study is beneficial for future ecological risk assessment of soil Cd contamination under divergent vegetation covers.

Measurement and modeling of hormesis in soil bacteria and fungi under single and combined treatments of Cd and Pb.

Heavy metals are considered major environmental pollutants. Soil microorganisms represent a predominant component of soils ecosystems, yet there is little information regarding hormetic responses of soil microorganisms to single and combined exposures to heavy metals. In the present study, to explore and predict the hormetic response of soil microorganisms, dose-response relationships of bacterial and fungal populations to single and combined treatments of cadmium (Cd) and lead (Pb) were evaluated. The results revealed hormetic responses of bacterial and fungal populations to both single and combined Cd and Pb treatments. The maximum stimulation (Mmax; relative to control treatment with no metals) of bacterial and fungal populations was 40% at 2 mg Cd/kg and 60% at 160 mg Pb/kg. An enhanced Mmax occurred in bacterial (50%) and fungal (75%) populations in the presence of the binary mixtures of 0.6 mg Cd/kg + 160 mg Pb/kg and 4.0 mg Cd/kg + 200 mg Pb/kg, suggesting positive additivity. This study showed that the hormetic effects of the mixtures were related to the independent effect of Cd and Pb, but they could not be predicted by the single effect of Cd or Pb. These new findings of the hormetic response of soil microorganisms to single treatments of Cd and Pb and their binary mixtures can facilitate the determination and minimization of ecological risks in heavy metal-polluted soils.

Hormetic responses of soil microbiota to exogenous Cd: A step toward linking community-level hormesis to ecological risk assessment.

We investigated hormetic responses of soil microbial communities to exogenous Cd by assessing microbial count, bacterial and fungal abundance, and microbial community diversity. We found that the bacterial count (BC) decreased (3-40%) by 0.2-40 mg Cdkg-1. Addition of 0.6-2.0 mgkg-1 significantly increased fungal count (FC) by 7-42%, while addition of 4.0-40 mgkg-1 Cd decreased FC by 29-51%, indicating a hormetic dose response. We also found that the FC/BC ratio increased by 0.6-2.0 mg Cdkg-1, with a maximum stimulation of 51%, and decreased (18-27%) by 4.0-40 mg Cdkg-1. Cd had no adverse effect on the α-diversity of bacterial or fungal communities. For relative abundances (RAs) of bacteria and fungi at phylum level, Bacteroidetes RA exhibited a biphasic dose-response curve, with an 18-24% increase at 0.6-4.0 mgkg-1 and a 10% decrease at 40 mgkg-1 compared with control. The results of FC, FC/BC, and Bacteroidetes RAs suggest that hormesis occurred at microbial community level, with positive effects occurring at 0.6-2.0 mgkg-1. This study can contribute to incorporating microbial community hormesis into the ecological risk assessments in the future.

The role of bacterial communities in shaping Cd-induced hormesis in 'living' soil as a function of land-use change.

Bacterial communities and soil physicochemical properties shape soil enzymes activities. However, how environmental factors and bacterial communities affect the relationship between increasing doses of soil pollutants and soil alkaline phosphatase (ALP), an index of soil microbiota activity, remains poorly understood. In this study, we investigated the response of soil ALP to 13 doses of Cd (0 and 0.01-100 mg/kg) under four land uses, viz. grassland (GL), natural forest (NF), plantation forest (PF), and wheat field (WF). We found that Cd commonly induced hormetic-like responses of soil ALP, with a maximum stimulation of 10.7%, 10.1%, 11.6%, and 14.5% in GL, NF, PF, and WF, respectively. The size of the hormetic zone (Horzone), an integrated indicator of the stimulation phase and biological plasticity, was in the order GL > WF > PF > NF, and the hormetic zone occurred in the dose range of 5-10, 0.3-10, 0.8-3, and 3-5 mg/kg, respectively. These results indicate highly pleiotropic responses of 'living' soil system to promote resilience to Cd contamination, with soil microbiota potentially contributing to soil ALP's hormetic-like response under different land uses. The hormetic-like response of 'living' soil ALP in different land uses offers a new insight into the identification and minimization of the ecological risks of land-use change in Cd-contaminated lands.

Cd induced biphasic response in soil alkaline phosphatase and changed soil bacterial community composition: The role of background Cd contamination and time as additional factors.

Hormesis is an intriguing phenomenon characterized by low-dose stimulation and high-dose inhibition. The hormetic phenomena have been frequently reported in the past decades, but the researches on the biphasic responses of soil enzymes are still limited. The main objective of this study is to explore dose response of alkaline phosphatase (ALP) to Cd (0, 0.003, 0.03, 0.3, 3.0 and 30 mg/kg) in the presence of different levels of background Cd contamination (bulk soil with no added Cd, BS; low background Cd, LB; medium background Cd, MB; and high background Cd, HB). ALP activity at 0.003-0.3 mg Cd/kg was 13-39% higher than that of the control (0 mg Cd/kg) for HB after 7 d. Similarly, the enzyme activities at 0.003-0.03 mg Cd/kg were 2-25% and 14-17% higher than those of the controls for MB and HB after 60 d. After 90 d, ALP activities at 0.3-3.0 mg Cd/kg increased by 11-17% for LB. The dose-response curves had the shape of an inverted U, showing biphasic responses at days 7 (HB), 60 (MB and HB) and 90 (LB). After 60 days of exposure, total operational taxonomic units (OTU) numbers and unique species exposed to Cd stress displayed hormetic-response curve for MB. The relative abundances of Agrobacterium, Salinimicrobiums, Bacilllus, and Oceanobacillus displayed significantly positive correlations with ALP activity. This suggested that bacterial communities potentially contribute to ALP's hormesis. This study further provides new insights into the ecological mechanisms of pollutant-induced hormesis, and substantially contributes to the ecological risk assessment of Cd pollution.

Toluene induces hormetic response of soil alkaline phosphatase and the potential enzyme kinetic mechanism.

Hormesis of soil enzyme that involved in heavy metal has been attracting much more attention for risk assessment of heavy metal toxicity, but insufficient studies were conducted to define the hormetic responses induced by toluene or other organic pollutions. The objectives of this study were to investigate the hormetic responses of soil enzyme induced by toluene and explore the potential enzyme kinetic mechanism. Soil alkaline phosphatase (ALP) activity was regarded as the endpoint to explore the hormetic responses under different doses of toluene (0.0, 0.1, 0.5, 1.0, 2.0, 3.0, 5.0, 10.0, 50.0 and 100.0 µL g-1). Subsequently, we conducted the experiments of enzymatic reaction kinetics and pure enzyme to further verify the potential mechanisms of soil ALP's hormesis. Results showed that ALP activities at 0.1-1.0 µL g-1 toluene were significantly increased in contrast to the control (0 µL g-1 toluene) (P < 0.05) at the exposure time of 30, 36, 48 and 54 h, with the maximum stimulation magnitudes of 24-43%. ALP activities were almost not affected by toluene (2-100 µL L-1) in the whole experimental period (6-54 h). Meanwhile, the values of catalytic efficiency (the radio Vmax/Km, Vmax: maximum reaction velocity and Km: Michaelis constant) and Vmax significantly increased compared with the control, but the value of Km decreased from 2.5 to 1.6. Overall, low dose toluene can induce hormesis of soil ALP. The potential reason is that low-dose toluene could enhance the combination of soil ALP and substrates. We believe that this study will provide a new viewpoint for ecological risk assessment of toluene contaminated soils.

Lysobacter may drive the hormetic effects of Pb on soil alkaline phosphatase.

It has become increasingly recognized that hormesis phenomena exist in soil ecosystem, but the research on the hormetic responses of soil enzymes are still limited. This study was conducted to investigate the hormetic effects of lead (Pb) on the activity of soil alkaline phosphatase (ALP) and the associated microbial groups. Soils were treated by adding Pb (NO3)2 solution with 0, 10, 100, 500, 1000, 2000, 4000, and 5000 mg/kg of Pb, respectively. A moist heat sterilization method (121 °C × 30 min) was used to discriminate the microbial effect on soil ALP hormesis from other factors. The bacterial community composition and abundance in the control (CK) and Pb-treated soils were detected by the high-throughput sequencing technique. The ALP activity at doses of 500-1000 mg/kg of Pb was significantly higher than that of CK (0 mg/kg of Pb), showing a typical inverted U-shaped dose response with the stimulation magnitude of 9.8-10.3% within 48 h of incubation. In addition, ALP activity decreased by 80% on average after soil sterilization. Analysis of bacterial community composition indicated that the relative abundance of Lysobacter at 1000 mg Pb/kg was higher than that of CK at genus level, with the increase of 69.82%. The highly significant correlation between soil ALP activities and relative abundance of Lysobacter indicated that this bacterial genus could possibly contribute to the hormetic responses of soil ALP to added doses of Pb in soils.

Time-Dependent Hormetic Response of Soil Alkaline Phosphatase Induced by Cd and the Association with Bacterial Community Composition.

Hormetic dose-response that involved Cd in soils is increasingly paid attentions for risk assessment of Cd toxicity, but insufficient studies were conducted to define the temporary modification of soil enzyme and the potential microbial responses. The present study chooses soil alkaline phosphatase (ALP) as endpoint to uncover the time-dependent hormetic responses to low doses of Cd and its association with bacterial community composition. The results showed that addition of 0.01-3.0 mg kg-1 Cd significantly increased ALP's activities with maximum stimulatory magnitude of 11.4-27.2%, indicating a typical hormesis. The response started at 12 h after Cd addition and maintained about 24 h. This demonstrated that the hormetic response is time-dependent and transient. Changes of soil bacterial community composition showed that, at 6 h, relative abundances (RAs) of Proteobacteria and Firmicutes at phylum and Pontibacter, Bacillaceae-Bacillus, Bacillaceae1-Bacillus, and Paenisporosarcina at genus significantly correlated with ALP's activities at 12-36 h (P < 0.05). This suggests that soil bacteria likely showed an earlier response to Cd and potentially contributes to the subsequent soil enzyme's hormesis. In addition, it was found that Gram-negative bacteria other than Gram-positive bacteria are prone to exhibiting a hormetic response under Cd stress. Our findings provide much insight into ecotoxicological risk assessment for soil Cd pollution.

Hormetic effects of Cd on alkaline phosphatase in soils across particle-size fractions in a typical coastal wetland.

Hormetic responses in soil ecosystem are increasingly reported recently. Soil enzymes are involved in almost all biochemical reactions, but insufficient investigations were conducted to define its hormetic responses. The objective of this study is to investigate the hormetic responses across soil particle-size fractions with cadmium (Cd) as a stressor and alkaline phosphatase (ALP) as a potential endpoint. Soils were treated by adding CdCl2·2.5H2O solution with 0, 0.003, 0.03, 0.3, 3.0 and 30.0mg·kg-1 of Cd, respectively. A low-power ultrasonic method was used to separate the bulk soil into 0.1-2, 2-63, 63-200 and 200-2000µm fractions. In 2-63µm, ALP activity at doses of 0.3-3.0mg·kg-1 of Cd was significantly higher than that of CK (0.0mg·kg-1 of Cd), showing a typical U-shaped dose-response with the amplitude of 72.3-118.6%. Similarly, ALP activity at 0.003-0.3mg·kg-1 of Cd was 36.4-66.1% higher than that of CK in 63-200µm. However, no similar phenomenon was observed in 0.1-2 and 200-2000µm fractions. This suggested that low doses of Cd induced the hormetic responses of soil ALP, particularly in 2-63 and 63-200µm. In addition, analysis of the microbial community structure and diversity indicates that, at genus level, the relative abundance (RA) of Gillisia at 0.03-0.3mg·kg-1 of Cd was significantly higher than that of CK with the amplitude of 3.7-37.5% in 2-63µm. The similar responses were observed that the RA of Pontibacter at 0.003-0.03mg·kg-1 of Cd was 4.0-85.4% higher than that of CK in 63-200µm. This showed that Gillisia and Pontibacter possibly contribute to the hormetic responses of soil ALP when low contents of Cd presented in soils. This study will provide a good insight into the hormetic phenomenon at soil ecosystem scales.