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Genet Mol Res ; 14(1): 2742-9, 2015 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-25867423

RESUMO

Adipocyte fatty acid-binding protein (A-FABP), the most abundant FABP in adipocytes, controls fatty acid uptake, transport, and metabolism in fat cells. We constructed a transgenic mice model that overexpressed the cattle A-FABP gene to investigate the relationship between A-FABP expression and intermuscular fat deposition. There was no significant difference in body weight and serum biochemical indexes between transgenic and wild-type mice. Further, there were no significant differences in intermuscular triglyceride content and A-FABP expression levels over three generations of transgenic mice. However, abdominal adipose rate, A-FABP protein content, and intermuscular triglyceride levels of transgenic mice were significantly higher than those of wild-type mice. In addition, triglycerides were remarkably higher in the skeletal muscle but lower in the myocardium of transgenic mice. Thus, overexpression of cattle A-FABP gene promoted fat deposition in the skeletal muscle of transgenic mice.


Assuntos
Adipócitos/metabolismo , Tecido Adiposo/metabolismo , Proteínas de Ligação a Ácido Graxo/metabolismo , Músculos/metabolismo , Gordura Abdominal/metabolismo , Animais , Bovinos , Ensaio de Imunoadsorção Enzimática , Gorduras/metabolismo , Proteínas de Ligação a Ácido Graxo/genética , Ácidos Graxos/metabolismo , Expressão Gênica , Camundongos Transgênicos , Músculo Esquelético/metabolismo , Miocárdio/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Triglicerídeos/metabolismo
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