The Rapid Non-Destructive Differentiation of Different Varieties of Rice by Fluorescence Hyperspectral Technology Combined with Machine Learning.

A rice classification method for the fast and non-destructive differentiation of different varieties is significant in research at present. In this study, fluorescence hyperspectral technology combined with machine learning techniques was used to distinguish five rice varieties by analyzing the fluorescence hyperspectral features of Thai jasmine rice and four rice varieties with a similar appearance to Thai jasmine rice in the wavelength range of 475-1000 nm. The fluorescence hyperspectral data were preprocessed by a first-order derivative (FD) to reduce the background and baseline drift effects of the rice samples. Then, a principal component analysis (PCA) and t-distributed stochastic neighborhood embedding (t-SNE) were used for feature reduction and 3D visualization display. A partial least squares discriminant analysis (PLS-DA), BP neural network (BP), and random forest (RF) were used to build the rice classification models. The RF classification model parameters were optimized using the gray wolf algorithm (GWO). The results show that FD-t-SNE-GWO-RF is the best model for rice classification, with accuracy values of 99.8% and 95.3% for the training and test sets, respectively. The fluorescence hyperspectral technique combined with machine learning is feasible for classifying rice varieties.

Distinguishing Different Varieties of Oolong Tea by Fluorescence Hyperspectral Technology Combined with Chemometrics.

Oolong tea is a semi-fermented tea that is popular among people. This study aims to establish a classification method for oolong tea based on fluorescence hyperspectral technology(FHSI) combined with chemometrics. First, the spectral data of Tieguanyin, Benshan, Maoxie and Huangjingui were obtained. Then, standard normal variation (SNV) and multiple scatter correction (MSC) were used for preprocessing. Principal component analysis (PCA) was used for data visualization, and with tolerance ellipses that were drawn according to Hotelling, outliers in the spectra were removed. Variable importance for the projection (VIP) > 1 in partial least squares discriminant analysis (PLS−DA) was used for feature selection. Finally, the processed spectral data was entered into the support vector machine (SVM) and PLS−DA. MSC_VIP_PLS−DA was the best model for the classification of oolong tea. The results showed that the use of FHSI could accurately distinguish these four types of oolong tea and was able to identify the key wavelengths affecting the tea classification, which were 650.11, 660.29, 665.39, 675.6, 701.17, 706.31, 742.34 and 747.5 nm. In these wavelengths, different kinds of tea have significant differences (p < 0.05). This study could provide a non-destructive and rapid method for future tea identification.