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1.
Zhonghua Er Ke Za Zhi ; 61(4): 345-350, 2023 Apr 02.
Artigo em Chinês | MEDLINE | ID: mdl-37011981

RESUMO

Objective: To analyze the clinical and genetic characteristics of pediatric patients with dual genetic diagnoses (DGD). Methods: Clinical and genetic data of pediatric patients with DGD from January 2021 to February 2022 in Peking University First Hospital were collected and analyzed retrospectively. Results: Among the 9 children, 6 were boys and 3 were girls. The age of last visit or follow-up was 5.0 (2.7,6.8) years. The main clinical manifestations included motor retardation, mental retardation, multiple malformations, and skeletal deformity. Cases 1-4 were all all boys, showed myopathic gait, poor running and jumping, and significantly increased level of serum creatine kinase. Disease-causing variations in Duchenne muscular dystrophy (DMD) gene were confirmed by genetic testing. The 4 children were diagnosed with DMD or Becker muscular dystrophy combined with a second genetic disease, including hypertrophic osteoarthropathy, spinal muscular atrophy, fragile X syndrome, and cerebral cavernous malformations type 3, respectively. Cases 5-9 were clinically and genetically diagnosed as COL9A1 gene-related multiple epiphyseal dysplasia type 6 combined with NF1 gene-related neurofibromatosis type 1, COL6A3 gene-related Bethlem myopathy with WNT1 gene-related osteogenesis imperfecta type XV, Turner syndrome (45, X0/46, XX chimera) with TH gene-related Segawa syndrome, Chromosome 22q11.2 microduplication syndrome with DYNC1H1 gene-related autosomal dominant lower extremity-predominant spinal muscular atrophy-1, and ANKRD11 gene-related KBG syndrome combined with IRF2BPL gene-related neurodevelopmental disorder with regression, abnormal movement, language loss and epilepsy. DMD was the most common, and there were 6 autosomal dominant diseases caused by de novo heterozygous pathogenic variations. Conclusions: Pediatric patients with coexistence of double genetic diagnoses show complex phenotypes. When the clinical manifestations and progression are not fully consistent with the diagnosed rare genetic disease, a second rare genetic disease should be considered, and autosomal dominant diseases caused by de novo heterozygous pathogenic variation should be paid attention to. Trio-based whole-exome sequencing combining a variety of molecular genetic tests would be helpful for precise diagnosis.


Assuntos
Anormalidades Múltiplas , Doenças do Desenvolvimento Ósseo , Deficiência Intelectual , Atrofia Muscular Espinal , Distrofia Muscular de Duchenne , Anormalidades Dentárias , Humanos , Estudos Retrospectivos , Deficiência Intelectual/genética , Doenças do Desenvolvimento Ósseo/complicações , Anormalidades Dentárias/complicações , Fácies , Distrofia Muscular de Duchenne/diagnóstico , Distrofia Muscular de Duchenne/genética , Distrofia Muscular de Duchenne/complicações , Atrofia Muscular Espinal/complicações , Proteínas de Transporte , Proteínas Nucleares
2.
Zhonghua Er Ke Za Zhi ; 61(2): 154-158, 2023 Feb 02.
Artigo em Chinês | MEDLINE | ID: mdl-36720598

RESUMO

Objective: To investigate the clinical features and gene variation characteristics of children with dynein cytoplasmic 1 heavy chain 1 (DYNC1H1) gene associated spinal muscular atrophy with lower extremity predominant (SMALED) 1. Methods: The clinical data of 4 SMALED1 children admitted to Peking University First Hospital from December 2018 to May 2021, who were found to have pathogenic variation of DYNC1H1 gene through genetic testing, except for other genes known to be related to motor retardation, were retrospectively summarized to analyze the phenotype and genotype characteristics. Results: There were 3 males and 1 female. The age of onset was 1 year, 1 day, 1 day and 4 months, respectively. The age of diagnosis was 4 years and 10 months, 9 months, 5 years and 9 months, and 3 years and 1 month, respectively. The clinical manifestations were muscle weakness and muscular atrophy of lower limbs, 2 cases with foot deformity, 1 case with early non progressive joint contracture, 1 case with hip dislocation and 1 case with mental retardation. De novo heterozygous missense variations in DYNC1H1 gene were found in all 4 children. According to the rating of American College of medical genetics and genomics, they were all possible pathogenic and pathogenic variations, with p.R598C, p.P776L, p.Y1109D variations had been reported, and p.I1086R variation had not been reported. Conclusions: For those with unexplained lower limb muscle weakness, muscle atrophy, joint contracture and foot deformity, upper limb motor ability related retention, with or without mental retardation, as well as the motor ability progresses slowly, it is necessary to consider the possibility of SMALED1 and the detection of DYNC1H1 gene when necessary.


Assuntos
Contratura , Deficiência Intelectual , Atrofia Muscular Espinal , Feminino , Masculino , Humanos , Estudos Retrospectivos , Atrofia Muscular Espinal/genética , Extremidade Inferior , Debilidade Muscular , Atrofia Muscular , Dineínas do Citoplasma/genética
3.
Zhonghua Fu Chan Ke Za Zhi ; 57(6): 419-425, 2022 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-35775249

RESUMO

Objective: To determine the effects of menopausal stage, age and other associated risk factors on symptoms of anxiety and depression among women in a community in Beijing. Methods: This study was a community-based prospective cohort. Participants who had transitioned through natural menopause, completed two or more depressive and anxiety symptoms evaluations, aged 35 to 64 years, and did not use hormone therapy were selected from the Peking Union Medical College Hospital aging longitudinal cohort of women in midlife to this analysis. The primary outcome variables were depressive and anxiety symptoms, assessed by hospital anxiety and depression scale (HADS). The generalized estimation equation was used in the statistical analysis. Results: Followed up from 2006 to 2014, 430 women and 2 533 HADS assessments were retained in the cohort. Depressive symptoms were more common than anxiety symptoms during all menopausal stages. The incidences of depressive and anxiety symptoms were 14.5% (19/191) and 3.1% (4/191) in the premenopausal -3 stage, respectively. The incidence increased in both menopausal transition and postmenopausal stage, with the highest incidence in the +1c stage [20.6% (155/751) and 8.8% (66/751), respectively]. However, these differences were not statistically significant (all P>0.05). Depressive symptoms were highest in the ≥60-<65 age group [20.8% (74/355)], and anxiety symptoms were highest in the ≥50-<55 age group [8.2% (62/754)]; but there were no statistical significances between different age groups and depressive and anxiety symptoms (all P>0.05). Multivariable analysis showed that high body mass index, low education status, and poor health status were independently associated with depressive symptoms (all P<0.05), and that poor health status, trouble falling asleep, and early awakening were independently associated with anxiety symptoms (all P<0.01). Conclusions: Depressive and anxiety symptoms are more common during menopausal transition and postmenopausal stage compared with reproductive stage. Depressive symptoms are more common than anxiety symptoms. To screen and assess depressive and anxiety symptoms in perimenopausal women is essential, especially for women with high risk factors.


Assuntos
Depressão , Menopausa , Ansiedade/epidemiologia , Pequim/epidemiologia , Depressão/diagnóstico , Depressão/epidemiologia , Feminino , Humanos , Pessoa de Meia-Idade , Estudos Prospectivos
4.
Zhonghua Yu Fang Yi Xue Za Zhi ; 55(2): 271-276, 2021 Feb 06.
Artigo em Chinês | MEDLINE | ID: mdl-34645192

RESUMO

Bacterial resistance is a serious problem in use of antibiotics and an urgent global public health challenge. The drug-resistant bacteria and drug-resistant genes carried by migratory birds are not only related to clinical antibiotics, but also the use of pesticides and veterinary drugs as well as the pollution of the surrounding environment of drug factories. However, studies on drug-resistant genes carried in migratory birds have been gradually reported around the world. Migratory birds have the characteristics of large range of movement and long flight distance, which leads to the complexity of bacterial resistance. Under the influence of environment and human activities, drug-resistant genes carried in bacteria are transmitted among species,human beings, domestic animals, environment and wild birds through mobile elements. This study summarizes the current situation of antibiotic resistance bacteria carried by migratory birds,the status of drug-resistant genes in migratory birds and the relationship between the resistance of migratory birds and the environment and human activities. The aim is to better understand the important role of migratory birds as hosts and vectors in the global spread of antibiotic resistance.


Assuntos
Antibacterianos , Aves , Animais , Animais Selvagens , Antibacterianos/farmacologia , Bactérias/genética , Farmacorresistência Bacteriana/genética , Resistência Microbiana a Medicamentos/genética , Humanos
5.
Zhonghua Liu Xing Bing Xue Za Zhi ; 41(9): 1420-1423, 2020 Sep 10.
Artigo em Chinês | MEDLINE | ID: mdl-33076591

RESUMO

Objective: Based on an investigation of an outbreak of COVID-19 in Nanchang, to understand the transmission process, analyze the infectivity of the cases in incubation period and asymptomatic carrier, and evaluate the transmission risks in different exposures. Methods: Case investigation was based on the traditional epidemiological survey, combined with analysis based on big data about population movement trajectories. Transmission chain was identified to indicate transmission relationship. Results: A total of 27 cases were found in this cluster epidemic, including 25 confirmed cases, 1 suspected case (index case) and 1 asymptomatic carrier. A total of 347 close contacts were found. The secondary attack rate was 7.2% (25/347). The infection rates in close contacts of the first, second, third and fourth generation cases were 52.6% (10/19), 6.1% (13/213), 2.3% (2/88) and 0.0% (0/27), respectively. Asymptomatic carrier caused household transmission. The infection rates in close contacts after having meals, sharing rooms/beds, having work contacts, having neighbor contacts, having same time medical services or sharing wards and sharing vehicles with the patients were 10.6%(17/160), 10.0%(20/201), 5.3%(5/94), 0.0%(0/30), 0.0%(0/18) and 0.0%(0/17), respectively. Conclusions: The infection source of this cluster epidemic was a suspected case from Wuhan. Analysis based on big data about population movement trajectories can help to search the cases and close contacts accurately. The proposed epidemic prevention and control measures based on this investigation were effective.


Assuntos
Infecções por Coronavirus/transmissão , Epidemias , Pneumonia Viral/transmissão , COVID-19 , China/epidemiologia , Análise por Conglomerados , Infecções por Coronavirus/epidemiologia , Humanos , Pandemias , Pneumonia Viral/epidemiologia
6.
Eur Rev Med Pharmacol Sci ; 24(17): 8940-8946, 2020 09.
Artigo em Inglês | MEDLINE | ID: mdl-32964984

RESUMO

OBJECTIVE: The aim of this study was to elucidate the role of FOXC2-AS1 in promoting the proliferative ability and inhibiting apoptosis of melanoma by silencing p15, thereafter regulating the progression of melanoma. PATIENTS AND METHODS: FOXC2-AS1 levels in melanoma patients with or without metastasis and those with the tumor in different stages were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Regulatory effects of FOXC2-AS1 on viability and apoptosis in melanoma cells were assessed, and subcellular distribution of FOXC2-AS1 was analyzed. Subsequently, the interactions of FOXC2-AS1 with EZH2 and SUZ12 were explored by RNA-Binding Protein Immunoprecipitation (RNA-RIP) assay. Through chromatin immunoprecipitation (ChIP) assay, the role of FOXC2-AS1 to regulate p15 transcription by recruiting EZH2 was verified. At last, regulatory effects of FOXC2-AS1/p15 axis on viability and apoptosis in melanoma cells were investigated. RESULTS: It was found that FOXC2-AS1 was upregulated in melanoma tissues, especially those with metastasis or stage II-IV. Melanoma patients expressing high level of FOXC2-AS1 showed worse survival than those with low level. Knockdown of FOXC2-AS1 inhibited viability, and stimulated apoptosis in A375 and sk-mel-110 cells. Besides, P15 level was upregulated in melanoma cells transfected with si-FOXC2-AS1, and FOXC2-AS1 was mainly distributed in cytoplasm. RNA-RIP assay confirmed that FOXC2-AS1 was mainly enriched in anti-EZH2 and aniti-SUZ12. Knockdown of EZH2 could markedly upregulate protein level of p15 in melanoma cells. Furthermore, it was verified that FOXC2-AS1 inhibited p15 transcription via recruiting EZH2, and the knockdown of p15 could partially reverse the regulatory effects of FOXC2-AS1 on viability and apoptosis in melanoma. CONCLUSIONS: FOXC2-AS1 stimulates proliferative ability in melanoma via silencing p15.


Assuntos
Inibidor de Quinase Dependente de Ciclina p15/metabolismo , Proteína Potenciadora do Homólogo 2 de Zeste/metabolismo , Melanoma/metabolismo , RNA Longo não Codificante/metabolismo , Neoplasias Cutâneas/metabolismo , Proliferação de Células , Inibidor de Quinase Dependente de Ciclina p15/genética , Proteína Potenciadora do Homólogo 2 de Zeste/genética , Humanos , Melanoma/patologia , RNA Longo não Codificante/genética , Neoplasias Cutâneas/patologia , Células Tumorais Cultivadas
8.
Zhonghua Er Ke Za Zhi ; 58(5): 408-412, 2020 May 02.
Artigo em Chinês | MEDLINE | ID: mdl-32392958

RESUMO

Objective: To explore the clinical, pathological and genetic characteristics of early-onset facioscapulohumeral muscular dystrophy type 1 (FSHD1), in order to increase awareness of the disease. Methods: In this retrospective study, the history of 3 patients, who were diagnosed with early-onset FSHD1 by molecular genetic test in Pediatric Outpatient Department of Peking University First Hospital from 4(th) June 2012 to 4(th) June 2018, were collected. Their clinical data, genotypes, phenotypes and pathological features of muscle biopsy were analyzed. Results: All the three patients were males at the age of 14 years, 11 years and 9 years 11 months, respectively, whose onset age was between infancy and early childhood and they got confirmed diagnosis within 4 to 10 years after the onset of illness. Their molecular genetic testing indicated that the number of D4Z4 repeat arrays located in 4qA were 2, 3 and 4, which was consistent with the characteristics of early-onset FSHD1. Their common clinical manifestations were facial, scapular and proximal lower limb muscle progressively and asymmetrically weakness. All patients had different severity of spine deformity and high-frequency dominant sensorineural hearing loss, however, the phenotype of the third patient with 4 D4Z4 repeats was significantly the most severe. Conclusions: Early-onset FSHD1 usually concealed onset and is difficult to diagnose. Its precise diagnosis depends on molecular genetic techniques, but the genotypes of 3 patients here are not corresponding to phenotypes strictly and it is necessary to accumulate more cases for further analysis in order to provide a more reliable basis for the relationship of genotype-phenotype and prognosis evaluation of the disease.


Assuntos
Distrofia Muscular Facioescapuloumeral/diagnóstico , Distrofia Muscular Facioescapuloumeral/genética , Adolescente , Criança , Genótipo , Humanos , Masculino , Fenótipo , Prognóstico , Estudos Retrospectivos
10.
Bioinspir Biomim ; 15(3): 036014, 2020 03 31.
Artigo em Inglês | MEDLINE | ID: mdl-32079775

RESUMO

The amazing multi-modal locomotion of flying squid helps to achieve fast-speed migration and predator-escape behavior. Observation of flying squid has been rarely reported in recent years, since it is challenging to clearly record the flying squid's aquatic-aerial locomotion in a marine environment. The existing reports of squid-flying events are rare and merely record the in-air motion. Therefore, the water-air locomotor transition of flying squid is still unknown. This paper proposes the idea of using CFD to simulate the process of the flying squid (Sthenoteuthis oualaniensis (S. oualaniensis)) launching from water into air. The results for the first time reveal the flow field information of squid in launching phase and show the kinematic parameters of flying squid in quantification. Both a trailing jet and pinch-off vortex rings are formed to generate launching thrust, and the formation number L ω /D ω is 5.22, demonstrating that the jet strategy is to produce greater time-averaged thrust rather than higher propulsion efficiency. The results also indicate that the maximum flying speed negatively correlates with the launch angle, indicating that a lower launch angle could result in a larger flying speed for the flying squid to escape. These findings explore the multi-modal locomotion of flying squid from a new perspective, helping to explain the trade-off strategy of water-to-air transition, and further enhance the performance of aquatic-aerial vehicles.


Assuntos
Comportamento Animal/fisiologia , Decapodiformes/fisiologia , Animais , Fenômenos Biomecânicos , Simulação por Computador , Locomoção , Natação
11.
Int J Biol Macromol ; 123: 622-628, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30447364

RESUMO

The property of biomaterials to control the sequential release of growth factors has been widely concerned in the field of bone repair and regeneration. Double-factor sustained-release scaffolds have good biocompatibility and bioactivity. O­Carboxymethyl chitosan microspheres (O-CMCS) has antimicrobial activity, biodegradability, biocompatibility and film formation. This study developed scaffolds materials with microspheres with a purpose of forming a controlled slow release secondary structure. O-CMCS were used as drug carrier to construct the compound sustained-release system with rhBMP-2 and VEGF double factors release. The O-CMCS were loaded with rhBMP-2 and the hydroxyapatite collagen (HC) scaffolds were loaded with VEGF to prepare the scaffolds. These were double factors composite sustained-release system scaffolds. The biocompatibility of the sustained-release system was evaluated by in vitro and in vivo experiments. HC composite scaffolds were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction detection (XRD), in vitro sustained release test, in vitro cell culture and in vivo animal experiments. The results showed that rhBMP-2/VEGF in HC composite scaffolds (HCBV) successfully achieved the sequential release of the double factors, which could benefit bone regeneration.


Assuntos
Materiais Biocompatíveis/química , Proteína Morfogenética Óssea 2/administração & dosagem , Regeneração Óssea/efeitos dos fármacos , Engenharia Tecidual , Fator de Crescimento Transformador beta/administração & dosagem , Fator A de Crescimento do Endotélio Vascular/administração & dosagem , Animais , Materiais Biocompatíveis/administração & dosagem , Materiais Biocompatíveis/síntese química , Proteína Morfogenética Óssea 2/química , Proteína Morfogenética Óssea 2/genética , Regeneração Óssea/genética , Osso e Ossos/efeitos dos fármacos , Quitosana/análogos & derivados , Quitosana/química , Colágeno/administração & dosagem , Colágeno/química , Liberação Controlada de Fármacos/efeitos dos fármacos , Durapatita/síntese química , Durapatita/química , Humanos , Microscopia Eletrônica de Varredura , Microesferas , Osteogênese/efeitos dos fármacos , Ratos , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Espectroscopia de Infravermelho com Transformada de Fourier , Alicerces Teciduais/química , Fator de Crescimento Transformador beta/química , Fator de Crescimento Transformador beta/genética , Fator A de Crescimento do Endotélio Vascular/química , Fator A de Crescimento do Endotélio Vascular/genética
12.
Comput Methods Biomech Biomed Engin ; 21(5): 413-426, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29974805

RESUMO

Both finite element models and multi-body models of human head-neck complex had been widely used in neck injuries analysis, as the former could be used to generate detailed stress strain information and the later could generate dynamic responses with high efficiency. Sometimes, detailed stress and strain information were hoped to be obtained more efficiently, but current methods were not effective enough when they were used to analyze responses of human head neck complex to long duration undulate accelerations. In this paper, a two-step procedure for 'parallel' development and 'sequential' usage of a pair of human head neck models was discussed. The pair of models contained a finite element model and a multi-body model, which were developed based on the coupling 'parallel' procedure using the same bio-realistic geometry. After being validated using available data, the pair of human neck models were applied to analyze biomechanical responses of pilot's neck during arrested landing operation according to the 'sequential' procedure, because typical sustained undulate accelerations usually appeared during such processes. The results, including both kinematic and detailed biomechanical responses of human head-neck complex, were obtained with preferable efficiency. This research provided an effective way for biomechanical analysis of human head neck responses to sustained undulate accelerations.


Assuntos
Modelos Anatômicos , Pescoço/anatomia & histologia , Pescoço/fisiologia , Aceleração , Fenômenos Biomecânicos , Análise de Elementos Finitos , Humanos , Modelos Biológicos , Músculos/anatomia & histologia , Lesões do Pescoço/patologia , Lesões do Pescoço/fisiopatologia , Amplitude de Movimento Articular , Reprodutibilidade dos Testes , Coluna Vertebral/anatomia & histologia , Estresse Mecânico
13.
Int J Biol Macromol ; 113: 792-803, 2018 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-29529585

RESUMO

Owing to low bearing capacity and efficiency, traditional filters or adsorbents for removal of contaminants like crystal violet (CV) dye required frequent replacement. Besides, the combination of three-dimensional (3D) printing and bionics could break the constraints of traditional configuration. In this study, a novel depth-type hybrid polylactic acid (PLA)@graphene oxide (GO)/chitosan (CS) sponge filter with bionic fish-mouth structure was prepared and fabricated, assisted by 3D printing and double freeze-drying technology, according to the theories of vertical cross-step filtration and swirling flow. And GO/CS sponge and its filtering device were characterized by FITR, SEM, water adsorption and so on. Moreover, it was explained that the impact factors on dye removal mechanism, like GO content (or CS content), contact time, pH, temperature and bionic configuration. As a result, the bionic 3D filtering device demonstrated excellent removal efficiency (97.8±0.5% for CV) and GO/CS sponge exhibited higher strength (74.5±3.5MPa) at the condition of GO content of 9wt%, contact time of 46min, pH of 8 and 35°C, respectively. Therefore, the resulting 3D PLA@GO/CS sponge bionic filter via gravity and vortex driving provided new alternatives for effectively dye-water separation, and it showed great promise for application of biological macromolecules in adsorption.


Assuntos
Quitosana/química , Violeta Genciana/química , Violeta Genciana/isolamento & purificação , Grafite/química , Óxidos/química , Poliésteres/química , Adsorção , Corantes/química , Corantes/isolamento & purificação , Filtração , Concentração de Íons de Hidrogênio , Fenômenos Mecânicos , Modelos Moleculares , Conformação Molecular , Porosidade , Temperatura , Água/química , Poluentes Químicos da Água/química , Poluentes Químicos da Água/isolamento & purificação
14.
Zhonghua Er Ke Za Zhi ; 54(10): 756-760, 2016 Oct 02.
Artigo em Chinês | MEDLINE | ID: mdl-27784478

RESUMO

Objective: To analyze the clinical and magnetic resonance imaging (MRI) features of congenital muscular dystrophy (CMD) to improve the diagnostic level. Method: Clinical manifestations and thigh muscle MRI results of 8 cases of CMD diagnosed on genetic level from April 2013 to November 2015 were investigated. MRI was performed on the thigh muscles of all cases. Fatty infiltration of different muscles described in T1WI was graded to evaluate. Clinical symptoms and signs, as well as muscle MRI features were analyzed by statistical description. Result: Among these 8 cases, 2 cases were diagnosed with Ullrich congenital muscular dystrophy (UCMD), 1 case had rigid spine with muscular dystrophy type 1 (RSMD1), 1 case had LMNA related muscular dystrophy (L-CMD), 1 case had congenital muscular dystrophy 1C (MDC1C) and 3 cases had congenital muscular dystrophy 1A (MDC1A), with 4 were males and 4 females, aged from 0.9 year to 4.8 years (median age was 2.2 years). All of these 8 cases presented with muscle weakness and hypotonia from birth to within the first six months, together with delayed motor development and joint contractures. Some cases had spinal deformity or skin changes. Various degrees of fatty infiltration in gluteus maximus and thigh muscles were shown in all of the cases, and differences among CMD subtypes in the form of fatty infiltration were detected; muscle edema was present in 5 cases, and muscle atrophy in 7 cases. However, none of them has muscle hypertrophy. Semimembranous muscle absence was detected in 1 case. Conclusion: The clinical manifestations and thigh muscle MRI findings of CMD have some features, and vary in certain CMD subtypes. MRI examination combined with clinical features may provide useful information to select appropriate genetic or other diagnostic techniques, which may help clinicians to make accurate diagnosis.


Assuntos
Corpos de Mallory/patologia , Distrofias Musculares/patologia , Esclerose/patologia , Escoliose/patologia , Coxa da Perna/patologia , Criança , Pré-Escolar , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Músculo Esquelético , Distrofia Muscular do Cíngulo dos Membros
15.
Int J Sports Med ; 35(7): 615-24, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24920560

RESUMO

We investigated the effects of vibration (35 Hz, 45 Hz and 55 Hz) as countermeasure locally applied to unloading hind limbs on bone, muscle and Achilles tendon. 40 female Sprague Dawley rats were divided into 5 groups (n=8, each): tail-suspension (TS), TS plus 35 Hz/0.3 g vibration (TSV35), TS plus 45 Hz/0.3 g vibration (TSV45), TS plus 55 Hz/0.3 g vibration (TSV55) and control (CON). After 21 days, bone mineral density (BMD) and the microstructure of the femur and tibia were evaluated by µCT in vivo. The biomechanical properties of the femur and Achilles tendon were determined by a materials testing system. Ash weight of bone, isotonic contraction and wet weight of soleus were also investigated. 35 Hz and 45 Hz localized vibration were able to significantly ameliorate the decrease in trabecular BMD (expressed as the percentage change from TS, TSV35: 48.11%, TSV45: 31.09%), microstructure and ash weight of the femur and tibia induced by TS. Meanwhile, 35 Hz vibration significantly improved the biomechanical properties of the femur (57.24% bending rigidity and 41.66% Young's modulus vs. TS) and Achilles tendon (45.46% maximum load and 66.67% Young's modulus vs. TS). Additionally, Young's modulus of the femur was highly correlated with microstructural parameters. Localized vibration was useful for counteracting microgravity-induced musculoskeletal loss. In general, the efficacy of 35 Hz was better than 45 Hz or 55 Hz in tail-suspended rats.


Assuntos
Densidade Óssea , Vibração , Tendão do Calcâneo/patologia , Animais , Atrofia/prevenção & controle , Fenômenos Biomecânicos , Feminino , Fêmur/anatomia & histologia , Fêmur/fisiologia , Membro Posterior , Elevação dos Membros Posteriores , Contração Muscular , Músculo Esquelético/patologia , Osteoporose/etiologia , Osteoporose/prevenção & controle , Condicionamento Físico Animal , Ratos Sprague-Dawley , Resistência à Tração , Tíbia/anatomia & histologia , Tíbia/fisiologia , Vibração/uso terapêutico , Ausência de Peso/efeitos adversos
16.
J Musculoskelet Neuronal Interact ; 13(2): 166-77, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23728103

RESUMO

Human performance in microgravity is characterized by reversed skeletal muscle actions in terms of active vs. passive mode contractions of agonist/antagonist groups that may challenge principal biodynamics (biomechanical forces translated from muscle to bone) of the skeletal muscle-bone unit. We investigated active vs. passive muscle motions of the unloaded hindlimb skeletal muscle-bone unit in the 21 days tail-suspended (TS) rat using a newly designed stepper exercise device. The regimen included both active mode motions (TSA) and passive mode motions (TSP). A TS-only group and a normal cage group (CON) served as positive or negative controls. The muscle and bone decrements observed in TS-only group were not seen in the other groups except TSP. Active mode motions supported femur and tibia bone quality (5% BMD, 10% microtrabecular BV/TV, Tb.Th., Tb.N. parameters), whole soleus muscle/myofiber size and type II distribution, 20% increased sarcolemma NOS1 immunosignals vs. CON, with 25% more hybrid fiber formation (remodeling sign) for all TS groups. We propose a new custom-made stepper device to be used in the TS rat model that allows for detailed investigations of the unique biodynamic properties of the muscle-bone unit during resistive-load exercise countermeasure trials on the ground or in microgravity.


Assuntos
Osso e Ossos/anatomia & histologia , Osso e Ossos/fisiologia , Elevação dos Membros Posteriores/fisiologia , Músculo Esquelético/anatomia & histologia , Músculo Esquelético/fisiologia , Condicionamento Físico Animal/fisiologia , Absorciometria de Fóton , Animais , Fenômenos Biomecânicos/fisiologia , Peso Corporal/fisiologia , Osso e Ossos/diagnóstico por imagem , Eletromiografia , Imunofluorescência , Imuno-Histoquímica , Extremidade Inferior/fisiologia , Terapia Passiva Contínua de Movimento , Músculo Esquelético/diagnóstico por imagem , Óxido Nítrico Sintase Tipo I/metabolismo , Tamanho do Órgão , Ratos , Ratos Sprague-Dawley , Treinamento Resistido , Tíbia/anatomia & histologia , Tíbia/diagnóstico por imagem , Tíbia/fisiologia , Tomografia Computadorizada por Raios X
17.
Cytopathology ; 23(2): 108-13, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21294792

RESUMO

OBJECTIVE: The aim of this study was to evaluate the diagnostic utility of glucose transporter 1 (GLUT1) mRNA expression in bronchial brushing specimens from patients with lung cancer. METHODS: GLUT1 mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR) in SurePath(TM) liquid-based cytology bronchial brushing specimens from patients with lung cancer (n=76) and benign lung disease (n=154). RESULTS: Compared with patients with benign disease and compared with cytology, GLUT1 mRNA was found significantly more frequently in patients with all carcinomas, squamous cell carcinomas, adenocarcinomas and small cell carcinomas, as well as central, peripheral and diffuse carcinomas (P<0.01). Minor differences were noted in GLUT1 mRNA and cytology results between histological types and tumour location but were not statistically significant. The diagnostic performance of RT-PCR analysis of GLUT1 mRNA was significantly higher than cytology in terms of sensitivity (97.4 ± 3.6% versus 65.8 ± 10.7) and negative predictive value (98.6 ± 1.9%, versus 85.6 ± 5.1%) but specificity (90.9 ± 4.5%) and positive predictive value (84.1 ± 7.6%) were lower than cytology (100%). CONCLUSIONS: Using liquid-based cytology, RT-PCR can be performed on bronchial brushing specimens to detect GLUT1 mRNA expression, and may be a useful adjunct to cytology diagnosis. It was more sensitive than cytology but its lower specificity should be taken into account.


Assuntos
Brônquios/metabolismo , Brônquios/patologia , Técnicas Citológicas/métodos , Transportador de Glucose Tipo 1/genética , Neoplasias Pulmonares/genética , Neoplasias Pulmonares/patologia , Manejo de Espécimes/métodos , Adulto , Idoso , Feminino , Regulação da Expressão Gênica , Transportador de Glucose Tipo 1/metabolismo , Humanos , Masculino , Pessoa de Meia-Idade , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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