[Clinical evaluation of composite inlays in defective molars].

OBJECTIVE: To evaluate clinical effect of composite inlays in the defective molars. METHODS: A total of 200 defective molars from 163 patients were divided into two groups, including 100 molars of each. One group was restored with the direct composite inlays and another group with the traditional composite fillings. All the restorations were evaluated in oral cavity after 6-month and 5-year filling or insertion with United States public health service criterions. The data were analyzed using SPSS 11.0 software with the chi-square test. The significance level was set at 5%. RESULTS: In clinical service for 6 months, the successful rate of composite inlays was 91.8% (90/98) and the corresponding figure for traditional composite fillings was 91.8% (89/97), but there was no statistically significant difference (P > 0.05). In clinical service for 5 years, the successful rate of composite inlay was 87.9% (80/91), the corresponding figure for the traditional composite fillings being 67.4% (60/89) and the difference was statistically significant (P < 0.05). CONCLUSIONS: In clinical, the defective molars can be well restored with the direct composite inlays. Especially in the long-term clinical service, the composite inlays show significant superiority over the traditional composite fillings.

[Effect of focal adhesion kinase on biological characteristics of Tca8113 tongue squamous cell carcinoma].

OBJECTIVE: To investigate the effect of FAK on biological characteristics of tongue squamous cell carcinoma cell Tca8113. METHODS: Lipofect was used to transfect FAK into Tca8113 cell lines. The difference in expression of FAK between the transfected and non-transfected cell lines was detected by immunofluorescence assay. Then by use of transwell room and wash way, the difference in invasion and adhesion ability between the transfected and non-transfected cell lines was tested. MTT method was adopted in finding the change of proliferation. RESULTS: RT-PCR analysis showed that the FAK gene was downregulated by Tca8113 cells. FAK expression was significantly down regulated by the transfection of FAK ASODN (P<0.05). Antisense FAK ODN significantly inhibited proliferation, adhesion and the invasion of Tca8113 cells. CONCLUSION: FAK plays an important role on biological characteristics of tongue squamous cell carcinoma cell Tca8113.

[Expression and clinical significance of focal adhesion kinase in oral squamous cell carcinoma].

OBJECTIVE: To study the correlation between expression levels of focal adhesion kinase (FAK) in oral squamous cell carcinoma (OSCC) and clinical prognosis with pathologic parameters. METHODS: Eighty paraffin embedded tissue specimens of OSCC were studied by immunohistochemistry. The data was analyzed by SPSS statistical software. RESULTS: FAK was highly expressed in OSCC tissues, in contrast to none or a low expression in normal oral epithelial tissue, and the heterogeneous staining was mainly located in the cell membrane and cytoplasm. FAK overexpression significantly correlated with histological differentiation and TNM stage, lymphatic metastasis. Multivariate analysis revealed that overexpression of FAK was the independent prognostic factor. CONCLUSION: The overexpression of FAK in the peripheral area of OSCC may serve as a useful biological factor affecting prognosis.