RESUMO
The aim of the present study was to identify biomarkers in osteosarcoma (OS) cell serum by antibody microarray profiling, which may be used for OS diagnosis and therapy. An antibody microarray was used to detect the expression levels of cytokines in serum samples from 20 patients with OS and 20 healthy individuals. Significantly expressed cytokines in OS serum were selected when P<0.05 and fold change >2. An enzyme-linked immunosorbent assay (ELISA) was used to validate the antibody microarray results. Finally, classification accuracy was calculated by cluster analysis. Twenty one cytokines were significantly upregulated in OS cell serum samples compared with control samples. Expression of interleukin-6, monocyte chemoattractant protein-1, tumor growth factor-ß, growth-related oncogene, hepatocyte growth factor, chemokine ligand 16, Endoglin, matrix metalloproteinase-9 and platelet-derived growth factor-AA was validated by ELISAs. OS serum samples and control samples were distinguished by significantly expressed cytokines with an accuracy of 95%. The results demonstrated that expressed cytokines identified by antibody microarray may be used as biomarkers for OS diagnosis and therapy.
Assuntos
Biomarcadores Tumorais/sangue , Neoplasias Ósseas/sangue , Condroblastoma/sangue , Citocinas/sangue , Osteoblastoma/sangue , Adolescente , Adulto , Anticorpos/química , Biomarcadores Tumorais/genética , Neoplasias Ósseas/diagnóstico , Neoplasias Ósseas/genética , Neoplasias Ósseas/patologia , Estudos de Casos e Controles , Condroblastoma/diagnóstico , Condroblastoma/genética , Condroblastoma/patologia , Análise por Conglomerados , Citocinas/genética , Ensaio de Imunoadsorção Enzimática , Feminino , Expressão Gênica , Humanos , Masculino , Osteoblastoma/diagnóstico , Osteoblastoma/genética , Osteoblastoma/patologia , Análise Serial de Proteínas/normas , Sensibilidade e EspecificidadeRESUMO
Polychlorinated biphenyl (PCB) contents were analyzed in samples collected from facilities related to PCB-containing products or wastes in South Korea. Average concentrations of the atmospheric Σ(209) PCBs were 7420 (37.0-104,048)pgm(-3) and 16.8 (ND-34.2 )fg WHO-TEQ m(-3) in indoor air samples; and 1670 (106-13,382)pgm(-3) and 5.64 (ND-36.0) fg WHO-TEQ m(-3) in outdoor air samples. The highest levels were observed in indoor air samples from disposal facilities (7336-104,048 pg m(-3)), followed by production (330-25,057 pg m(-3)), recycling, and storage facilities, indicating that PCB emissions from PCB-containing products and wastes remains very high and the facilities related with those may be an important source to atmospheric PCBs. Principal component analysis of PCB profiles showed that the homologue patterns of PCBs in outdoor and indoor air samples collected from the facilities were similar to those of boundary air samples and PCB commercial products, e.g. Aroclor 1016, 1221, 1232 and 1242. Evaluation of the PCB mass balance in a facility, dismantling and solvent-washing PCB-contaminated transformers, showed that of the total PCBs treated in this facility, approximately 0.0022% was emitted to the atmosphere, and most was transferred to waste oil for disposal by incineration or chemical methods.
