Substance P combined with epidermal stem cells promotes wound healing and nerve regeneration in diabetes mellitus.

Exogenous substance P accelerates wound healing in diabetes, but the mechanism remains poorly understood. Here, we established a rat model by intraperitoneally injecting streptozotocin. Four wounds (1.8 cm diameter) were drilled using a self-made punch onto the back, bilateral to the vertebral column, and then treated using amniotic membrane with epidermal stem cells and/or substance P around and in the middle of the wounds. With the combined treatment the wound-healing rate was 100% at 14 days. With prolonged time, type I collagen content gradually increased, yet type III collagen content gradually diminished. Abundant protein gene product 9.5- and substance P-immunoreactive nerve fibers regenerated. Partial nerve fiber endings extended to the epidermis. The therapeutic effects of combined substance P and epidermal stem cells were better than with amniotic membrane and either factor alone. Our results suggest that the combination of substance P and epidermal stem cells effectively contributes to nerve regeneration and wound healing in diabetic rats.

[Rapid identification of Dendrobium plants based on near infrared diffuse reflection spectroscopy].

Near infrared diffuse reflection spectra of 15 species' 171 samples of Dendrobium combined with chemometrics statistical analysis were used to build prediction model, in order to discriminate different species of Dendrobium quickly and nondestructively. Hotelling T2 was applied to stability analysis of spectrum of 5 random drawing samples, and the results showed that the samples spectrum possessed good stability. Orthogonal test L24 (2 x 4 x 3 x 8) was designed to optimize optical path type, spectral band, derivative and smooth. The result of orthogonal test was analyzed by principal component analysis, which revealed that when 6500-4000 cm(-1) spectral band was applied, and with multiplicative scatter correction, second derivative, Norris smooth, and the number of principal components 7, the spectrum distinguishing accuracy was 100%. With the optimized condition of orthogonal test as the input value of partial least squares discriminant analysis and random drawing 123 samples as calibration set to establish the prediction model, and the rest 48 samples as prediction set were use to assess the property of the prediction model, the results indicated that the accumulating contribution rate of the first 3 principal components of the model was 99.36%, the identification of the standard deviation was +/- 0.1, and the correct recognition rate of the model was 97.92%. The results were satisfied. The method provided a new way for the rapid identification of different species of Dendrobium, and also supplied a reference for the authentication of medicinal plants.

[Direct determination of copper and manganese in Pinellia ternata by atomic absorption spectrometry using secondary sensitive wavelength].

A method for the direct determination of copper and manganese in Pinellia ternata by flame atomic absorption spectrometry with secondary sensitive wavelength was developed. This method can greatly decrease the analytical errors caused by small amount of samples orbig dilution multiple, so its accuracy is better than that with normal atomic absorption spectrometry using sensitive line when determining Cu-rich and Mn-rich Pinellia ternata samples. The detection limits of Cu-rich and Mn-rich are 0-25 mg x (-1) and 0-12 mg x L(-1), the relative standard deviations are 0.42% and 0.66%, and the recovery rates 96%-103% and 97%-102%, respectively. It was found that the method is simple, rapid, useful, accurate and free from the interference of coexistent ions. It has been widely applied to the determination of Pinellia ternata samples with satisfactory results.