RESUMO
OBJECTIVE: To investigate the biological characters of human skin fibroblasts in fibroblast populated collagen lattice (FPCL). METHODS: The human fibroblasts were cultured in 3D and the collagen of the rat tail was also prepared. They were examined with the comprising cell cycle and apoptosis, mRNA expression of TGF beta1, and fibronectin, and cell morphology. RESULTS: The flow cytometry showed that the G0/G1, stage cells were 79% +/- 3%, 87% +/- 2% after the 7 days and 14 days separately, and there were not apoptosis peak observed. RT-PCR analysis revealed that the mRNA expression of TGF beta1, and fibronectin had no difference between human skin fibroblasts cultured in 3D and 2D. Electron microscope showed the cells were plenty of chromatin and organelles. CONCLUSIONS: The proliferation of the human skin fibroblasts in FPCL is slow, but its biological viability is better.
Assuntos
Fibroblastos/citologia , Pele/citologia , Engenharia Tecidual/métodos , Animais , Técnicas de Cultura de Células , Divisão Celular , Células Cultivadas , Colágeno , Matriz Extracelular , Humanos , RatosRESUMO
OBJECTIVE: To search an ideal carrier of transferred keratinocytes for transplantation. METHODS: The transferred keratinocytes were seeded on the surfaces of the artificial dermis and the silicone membrane and cultured in vitro for 2 weeks. The growth of the keratinocytes was observed by microscope and scanning electron microscope. RESULTS: The keratinocytes implanted on the artificial dermis began to rupture and died after 2 to 3 days. While the keratinocytes adhered well on the surface of silicone membrane with pseudopodia formation after 1 week under scanning electron microscope, and the cells kept normal morphological and proliferative properties 2 weeks later. CONCLUSION: The silicone membrane can be applied as an useful carrier for the keratinocytes transplantation.
