RESUMO
OBJECTIVE: Pseudohypoparathyroidism type Ia (PHP-Ia) is a hereditary disorder characterized by resistance to multiple hormones that work via cAMP such as PTH and TSH, accompanied by typical skeletal features including short stature and brachydactyly, termed Albright hereditary osteodystrophy (AHO). In affected kindreds, some members may have AHO but not hormone resistance; they are termed as pseudopseudohypoparathyroidism (PPHP). The molecular basis for the disorder is heterozygous inactivating mutation of the Gsalpha gene. In affected families, subjects with both PHP-Ia and PPHP have the same Gsalpha mutations. The skeletal features common to PPHP and PHP-Ia are presumably caused by tissue-specific Gsalpha haploinsufficiency. Other features that distinguish between PPHP and PHP-Ia, such as the multihormone resistance, are presumably caused by tissue-specific paternal imprinting of Gsalpha. This suggests that major differences in phenotype between PHP-Ia and PPHP point to specific tissues with Gsalpha imprinting. One such major difference may be cognitive function in PHP-Ia and PPHP. DESIGN: Description of a large family with PHP-Ia and PPHP. PATIENTS: Eleven affected subjects with PHP-Ia or PPHP in one family. MEASUREMENTS: Cognitive impairment (CI) was defined by a history of developmental delay, learning disability and the Wechsler intelligence scale. RESULTS: CI occurred only in the five PHP-Ia but not in the six PPHP subjects. Hypothyroidism which occurred in all PHP-Ia subjects was apparently not the cause of CI as it was mild, and was treated promptly. Analysis of additional Israeli cases, and the published cases from the literature, all with documented Gsalpha mutations, revealed that CI is prevalent in PHP-Ia [60 of 77 subjects (79%)] but not in PPHP [3 of 30 subjects (10%)] (P < 1 x 10(-6)). CONCLUSION: We suggest that Gsalpha is imprinted in the brain.
Assuntos
Encéfalo/metabolismo , Transtornos Cognitivos/genética , Subunidades alfa Gs de Proteínas de Ligação ao GTP/genética , Impressão Genômica/genética , Pseudo-Hipoparatireoidismo/genética , Pseudopseudo-Hipoparatireoidismo/genética , Adolescente , Adulto , Criança , Pré-Escolar , Transtornos Cognitivos/etiologia , Feminino , Displasia Fibrosa Poliostótica/genética , Heterozigoto , Humanos , Lactente , Masculino , Mutação , Pseudo-Hipoparatireoidismo/fisiopatologia , Pseudopseudo-Hipoparatireoidismo/fisiopatologia , Adulto JovemRESUMO
BACKGROUND: WNK [With No K (lysine)] kinases are essential for regulation of blood pressure and potassium homeostasis. WNK4 expression was recently found not only in the distal nephron but also in chloride-transporting epithelia. To establish a physiological role for this distribution we studied patients with familial hyperkalaemia and hypertension (FHH), [pseudohypoaldosteronism type II (PHAII)], which is caused by mutations in WNK4. DESIGN: Measurement of nasal potential difference (NPD) and sweat electrolytes were performed in controls, in six subjects with FHH and ten subjects with cystic fibrosis (CF). RESULTS: Basal NPD was higher in FHH compared with controls (n = 20): 22.8 +/- 5.7 vs. 16.2 +/- 5.3 mV, respectively (P = 0.014). Maximal response to amiloride was also higher in FHH compared with controls: 14.8 +/- 3.5 vs. 10.0 +/- 4.8 mV, respectively (P = 0.03). In CF these values were 42.9 +/- 9.3 and 29.9 +/- 7.4 mV, respectively. The kinetics of the amiloride effect were faster in FHH, and as first reported here also in CF, compared with controls. At 30 s, amiloride-inhibitable residual PD in FHH was 50 +/- 30 vs. 81 +/- 9% in controls (P = 0.0003) and 56 +/- 7% in CF. The response to chloride-free and isoproterenol solutions, which determines chloride transport activity, was similar in FHH compared with controls [16.0 +/- 8.6 vs. 10.4 +/- 5.9 mV (P = 0.08)]. Sweat conductivity in FHH was 49.7 +/- 7.3 vs. 38.2 +/- 8.1 mmol (NaCl eq) L-1 in 16 controls (P = 0.007) and 94.0 +/- 19.3 in CF. CONCLUSIONS: Mutant WNK4 increases Na+ transport in airways, and therefore it is regulated by wild-type WNK4. This may be caused by a regulation of ENaC or a K+ channel.
Assuntos
Hiperpotassemia/fisiopatologia , Hipertensão/fisiopatologia , Proteínas Serina-Treonina Quinases/fisiologia , Sódio/metabolismo , Fibrose Cística/fisiopatologia , Transporte de ÍonsRESUMO
Antiphospholipid antibody syndrome (APS) is associated with adverse pregnancy outcomes and maternal complications including thrombotic events and early pre-eclampsia. HELLP syndrome (Hemolysis, Elevated Liver enzymes, Low Platelets) represents a unique form in the spectrum of pre-eclampsia. This report describes four patients with pregnancy-associated hepatic infarctions. All four had APS and HELLP syndrome, which was complete in one patient and incomplete in three patients, with elevated liver enzymes in all, and either thrombocytopenia or hemolysis in two. In the literature, we found descriptions of an additional 24 patients who had 26 pregnancies with concomitant hepatic infarction. Of the total 28 patients, anticardiolipin antibodies (aCL) and/or lupus anticoagulant (LAC) were assessed in 16 patients, out of whom 15 were found to be positive. Hepatic infartction during pregnancy was associated almost always with APS, with HELLP (2/3 complete, 1/3 incomplete), and only in one-third of the pregnancies with pre-eclampsia (PE).
Assuntos
Aborto Habitual/etiologia , Síndrome Antifosfolipídica/diagnóstico , Síndrome HELLP/diagnóstico , Hepatopatias/diagnóstico , Complicações Cardiovasculares na Gravidez/imunologia , Aborto Habitual/epidemiologia , Adulto , Síndrome Antifosfolipídica/complicações , Feminino , Síndrome HELLP/complicações , Humanos , Infarto/diagnóstico , Infarto/etiologia , Hepatopatias/complicações , Gravidez , Resultado da Gravidez , Trombose Venosa/diagnóstico , Trombose Venosa/etiologiaRESUMO
Gitelman's syndrome is manifested by hypokalemic alkalosis, hypomagnesemia, hypocalciuria, normotensive hyperreninemia and hyperaldosteronism. Hypokalemia can at times be refractory to treatment. We present a patient refractory to a variety of drugs including indomethacin, the nonspecific COX inhibitor. Rofecoxib, a specific COX 2 inhibitor, promptly elevated serum potassium concentration with normalization of plasma aldosterone and near normalization of renin without a change in serum magnesium. Our patient also had rhabdomyolysis, a rarely reported complication, which was also ameliorated by COX 2 inhibition.
Assuntos
Inibidores de Ciclo-Oxigenase/uso terapêutico , Hipopotassemia/tratamento farmacológico , Lactonas/uso terapêutico , Rabdomiólise/tratamento farmacológico , Adulto , Alcalose/tratamento farmacológico , Cálcio/urina , Humanos , Hiperaldosteronismo , Hipopotassemia/diagnóstico , Magnésio/sangue , Masculino , Rabdomiólise/diagnóstico , Sulfonas , Síndrome , Resultado do TratamentoRESUMO
BACKGROUND: An abnormal distribution of subsets of gammadelta T cells, which are a component of the inflammatory infiltrate in arthritic synovium, has been demonstrated in the peripheral blood (PB) of patients with arthritis and neutropenia. OBJECTIVE: To evaluate whether the clinical manifestations of patients with arthritis and neutropenia are related to the specific gammadelta T cell subset predominant in the PB. METHODS: Flow cytometry of PB lymphocytes in six consecutive patients with chronic neutropenia and arthritis was performed. Variable (V) gamma and delta gene families were analysed by polymerase chain reaction. cDNA was subjected to direct automated sequencing of T cell receptor (TCR) genes. RESULTS: Three patients had non-deforming and non-erosive rheumatoid factor (RF)(+) polyarticular rheumatoid arthritis, RF(+) oligoarticular arthritis, or RF(-) non-deforming oligoarticular psoriatic arthritis with persistent expansions of Vgamma1(+)/Vdelta2(+), Vgamma2(+)/Vdelta2(+), or Vgamma1(+)/Vdelta (undetermined (2- 1-)) T cells, respectively. The other three patients, without persistent expansion of gammadelta T cells, had either non-deforming and non-erosive oligo- or polyarthritis with a balanced distribution of several Vdelta and Vgamma genes, or severe erosive RF(+) arthritis with deficiency of all but Vgamma1(+)/Vdelta1(+) T cells. CONCLUSIONS: gammadelta T cell lymphoproliferations in chronic neutropenia and arthritis use different Vgamma and Vdelta gene families, often forming T cell receptor (TCR) structures that are infrequent in normal adult PB. Arthritis with Vgamma1(+)/Vdelta2(+), Vgamma2(+)/Vdelta2(+), or Vgamma1(+)/Vdelta2(-)/Vdelta1(-) gammadelta T cells in the PB is non-deforming and non-erosive, suggesting a protective effect of these cells, as opposed to a more pathogenic contribution of Vgamma1(+)/Vdelta1(+) cells.
Assuntos
Artrite/imunologia , Neutropenia/imunologia , Receptores de Antígenos de Linfócitos T gama-delta , Membrana Sinovial/imunologia , Subpopulações de Linfócitos T/imunologia , Idoso , Doença Crônica , Feminino , Citometria de Fluxo , Genes Codificadores dos Receptores de Linfócitos T , Humanos , Imunidade Celular , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da PolimeraseRESUMO
A unique patient who developed pseudoaneurysm of the ascending aorta after coronary artery bypass grafting is presented. This case is peculiar due to the presenting symptom being fever of unknown origin. It is the first description of a patient on hemodialysis, who developed ascending aortic pseudoaneurysm.
Assuntos
Falso Aneurisma/etiologia , Aneurisma da Aorta Abdominal/etiologia , Ponte de Artéria Coronária/efeitos adversos , Febre de Causa Desconhecida , Falso Aneurisma/cirurgia , Aneurisma da Aorta Abdominal/cirurgia , Evolução Fatal , Humanos , Masculino , Pessoa de Meia-Idade , Diálise RenalRESUMO
Hypertension is a major public health problem of largely unknown cause. Here, we identify two genes causing pseudohypoaldosteronism type II, a Mendelian trait featuring hypertension, increased renal salt reabsorption, and impaired K+ and H+ excretion. Both genes encode members of the WNK family of serine-threonine kinases. Disease-causing mutations in WNK1 are large intronic deletions that increase WNK1 expression. The mutations in WNK4 are missense, which cluster in a short, highly conserved segment of the encoded protein. Both proteins localize to the distal nephron, a kidney segment involved in salt, K+, and pH homeostasis. WNK1 is cytoplasmic, whereas WNK4 localizes to tight junctions. The WNK kinases and their associated signaling pathway(s) may offer new targets for the development of antihypertensive drugs.
Assuntos
Hipertensão/genética , Mutação , Proteínas Serina-Treonina Quinases/genética , Pseudo-Hipoaldosteronismo/genética , Sequência de Aminoácidos , Sequência de Bases , Mapeamento Cromossômico , Cromossomos Humanos Par 12/genética , Cromossomos Humanos Par 17/genética , Citoplasma/enzimologia , Feminino , Regulação Enzimológica da Expressão Gênica , Ligação Genética , Humanos , Hipertensão/enzimologia , Hipertensão/fisiopatologia , Junções Intercelulares/enzimologia , Peptídeos e Proteínas de Sinalização Intracelular , Íntrons , Túbulos Renais Coletores/enzimologia , Túbulos Renais Coletores/ultraestrutura , Túbulos Renais Distais/enzimologia , Túbulos Renais Distais/ultraestrutura , Masculino , Proteínas de Membrana/metabolismo , Microscopia de Fluorescência , Antígenos de Histocompatibilidade Menor , Dados de Sequência Molecular , Mutação de Sentido Incorreto , Linhagem , Fosfoproteínas/metabolismo , Proteínas Serina-Treonina Quinases/química , Proteínas Serina-Treonina Quinases/metabolismo , Pseudo-Hipoaldosteronismo/enzimologia , Pseudo-Hipoaldosteronismo/fisiopatologia , Deleção de Sequência , Transdução de Sinais , Proteína Quinase 1 Deficiente de Lisina WNK , Proteína da Zônula de Oclusão-1RESUMO
BACKGROUND: Trans-tubular potassium gradient (TTKG) is considered to reflect mainly aldosterone bioactivity with regard to its kaliuretic response. We determined both TTKG and aldosterone serum concentrations in patients with severe drug-induced hyperkalemia (DIH). METHODS: Ten hyperkalemic patients with serum potassium of more than 5.5 mEq/l, and serum creatinine of less than 2.5 mg/dl (221 micromol/l) were studied prospectively. Two control groups of 10 patients each were used. Control 1 group with normal renal function, and control 2 group with normokalemia and renal failure of the same magnitude as that of the hyperkalemic patients. Serum osmolarity, electrolytes, creatinine, aldosterone and urine electrolytes and osmolarity were measured and TTKG calculated. RESULTS: DIH patients had lower TTKG values than control 1 patients (2.58 +/- 0.36 vs. 6.68 +/- 0.55, p < 0.001), and also lower than that of the control 2 patients (2.58 +/- 0.36 vs. 5.51 +/- 0.87, p < 0.01). Serum aldosterone concentration in the DIH group was higher than that of the control 1 group [24.30 +/- 5.0 vs. 7.4 +/- 2.1 pg/ml (674 +/- 139 vs. 205 +/- 58 pmol/l), p < 0.006] but not different from that of the control 2 group [24.3 +/- 5.0 vs. 15.3 +/- 3.8 pg/ml (674 +/- 139 vs. 424 +/- 106 pmol/l), respectively, p = 0.18]. Although there was some overlap in TTKG between DIH and control groups, 6 of 10 DIH patients had TTKG of less than 2.5, while none of the control patients had such a low value. CONCLUSION: DIH is characterized by lower TTKG values than those observed in patients with normal or mild-to-moderate renal failure. Other factors in addition to aldosterone seem to be involved.
Assuntos
Hiperpotassemia/metabolismo , Túbulos Renais/metabolismo , Potássio/metabolismo , Idoso , Idoso de 80 Anos ou mais , Aldosterona/sangue , Inibidores da Enzima Conversora de Angiotensina/efeitos adversos , Captopril/efeitos adversos , Creatinina/sangue , Feminino , Humanos , Hiperpotassemia/induzido quimicamente , Masculino , Antagonistas de Receptores de Mineralocorticoides/efeitos adversos , Insuficiência Renal/metabolismo , Espironolactona/efeitos adversos , Uremia/sangueAssuntos
Ponte de Artéria Coronária , Síndrome Pós-Pericardiotomia/complicações , Síndrome do Desconforto Respiratório/complicações , Anti-Inflamatórios/uso terapêutico , Humanos , Hidrocortisona/uso terapêutico , Masculino , Pessoa de Meia-Idade , Síndrome Pós-Pericardiotomia/fisiopatologia , Radiografia , Síndrome do Desconforto Respiratório/diagnóstico por imagem , Síndrome do Desconforto Respiratório/tratamento farmacológico , Síndrome do Desconforto Respiratório/fisiopatologia , Resultado do TratamentoRESUMO
OBJECTIVE: To report the case of a patient with manic-depressive disorder who developed lithium intoxcation following carbamazepine-induced acute renal failure. CASE SUMMARY: A 33-year-old white man with bipolar manic-depressive disorder was treated with lithium for the last 18 months. Three weeks prior to admission, carbamazepine 600 mg was added to the drug regimen due to a recurrence of the psychiatric disorder. He was admitted wh signs of lithium intoxication. Acute renal failure due to carbamazepine-induced interstitial nephritis was diagnosed. DISCUSSION: The combination of carbamazepine and lithium is known to cause neurotoxicity. We describe a different interaction in which the toxic lithium concentrations were the result of carbamazepine-induced acute renal failure. CONCLUSIONS: When considering adding carbamazepine to lithium, careful follow-up of the patients is warranted to prevent this indirect drug in interaction.
Assuntos
Injúria Renal Aguda/induzido quimicamente , Anticonvulsivantes/efeitos adversos , Transtorno Bipolar/tratamento farmacológico , Carbamazepina/efeitos adversos , Lítio/efeitos adversos , Adulto , Interações Medicamentosas , Humanos , MasculinoAssuntos
Ética Médica , Holocausto , Judeus , Médicos , Sistemas Políticos , Alemanha , Humanos , Publicações Periódicas como Assunto , EditoraçãoRESUMO
Patients with intractable angina pectoris despite optimal drug therapy, who are not candidates for revascularization procedures, pose a very difficult problem. We evaluated the role of chronic opioid therapy in four such patients. The patients (mean age 79.5 years) were treated by low doses (mean 40 mg/day) of controlled-release oral morphine (CRM) for 1 to 5 years. The treatment was followed by a marked decline in the rate of admissions and hospitalization periods. The number of admissions decreased from a mean of 6 during the year prior to CRM therapy to 1.5 the following year. The duration of hospitalization for angina pectoris during these periods decreased from a mean of 42 +/- 35 days to 6 +/- 10 days (p < 0.05). Side effects were negligible and consisted mainly of lactulose-responsive constipation. We suggest that prolonged oral morphine therapy may be administered with good efficacy and no significant side effects in selected patients with intractable angina pectoris.
Assuntos
Analgésicos Opioides/uso terapêutico , Angina Pectoris/tratamento farmacológico , Angina Pectoris/fisiopatologia , Morfina/uso terapêutico , Administração Oral , Idoso , Idoso de 80 Anos ou mais , Preparações de Ação Retardada , Feminino , Hospitalização , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Fatores de TempoRESUMO
An acute severe onset of celiac disease is very uncommon in adults. We describe a patient with adult celiac disease who presented with acute diarrhea that lead rapidly to a life threatening hypokalemia and acidosis, the so-called celiac crisis. Celiac crisis, described mainly in children younger than two years of age, has become very rare due to earlier diagnosis and effective therapy of the disease. The case described is an example of the heterogeneous clinical course of celiac disease and emphasizes the need to consider it in the differential diagnosis, even in adults suffering from acute diarrhea and acidosis.
Assuntos
Acidose/etiologia , Doença Celíaca/dietoterapia , Doença Celíaca/diagnóstico , Diarreia/etiologia , Hipopotassemia/etiologia , Adulto , Doença Celíaca/complicações , Estado Terminal , Progressão da Doença , Endoscopia Gastrointestinal , Feminino , Seguimentos , Humanos , Resultado do TratamentoRESUMO
People without hemophilia but with autoantibodies specifically directed against the procoagulant activity of factor VIII are known to have acquired hemophilia. The bleeding diathesis in these patients is often severe and life-threatening. The definite laboratory diagnosis of this disorder includes demonstration of low factor VIII levels in plasma with a high titer of factor VIII inhibitors, but the initial suspicion for its presence should rise in view of a prolonged partial thromboblastin time (PTT) and a normal prothrombin time associated with an acquired bleeding disorder. Oral anticoagulant treatment is known to prolong PTT as well, and the merger of these 2 situations may cause delayed diagnosis of acquired hemophilia with devastating consequences. We describe here the first reported case of acquired hemophilia diagnosed in a patient treated with warfarin. In such patients prolonged PTT may be ascribed to warfarin therapy rather than to acquired hemophilia, thus causing a dangerous delay in diagnosis.
Assuntos
Anticoagulantes/efeitos adversos , Hemofilia A/diagnóstico , Varfarina/efeitos adversos , Idoso , Autoanticorpos/sangue , Transtornos da Coagulação Sanguínea/diagnóstico , Diagnóstico Diferencial , Fator VIII/imunologia , Feminino , Hemofilia A/sangue , Hemofilia A/imunologia , Humanos , Tempo de Tromboplastina ParcialRESUMO
One of the four types of Kaposi's sarcoma (KS), KS after organ transplantation under immunosuppression, is a well-known entity and has been abundantly described in renal, heart and liver recipients. We report the second case of cutaneous KS after lung transplantation, under regular immunosuppression, in a Sephardic Jewish woman. This case, when added to the other 10 cases of posttransplantation KS reported from Israel, all being Sephardic Jews, indicates that in Israel, Sephardic Jews are at higher risk than Ashkenazi Jews to develop posttransplantation KS. This observation should be added to the well-known increased risk of Ashkenazi Jews to develop classic KS. Moreover, in Israel Ashkenazi Jews develop classic KS at higher rates than Sephardic Jews. This apparent discrepancy in the ethnic distribution between Sephardic and Ashkenazi Jews in classic versus posttransplantation KS may shed light on the pathogenesis of KS in general.