Aminoglycoside resistance in domestic sewage and clinical Escherichia coli isolates

SUMMARY Introduction: Escherichia coli, a Gram-negative bacillus, is found in diverse environments and causes several human diseases, such as pneumonia and urinary tract infections. Aminoglycosides are antimicrobials that present high activity against Gram-negative species, including multidrug-resistant pathogens. However, the indiscriminate use of these compounds has selected resistant microorganisms, mainly due to the production of aminoglycoside-modifying enzymes (AME). Material and methods: The minimal inhibitory concentration of the aminoglycosides amikacin, gentamicin, and neomycin against clinical (CI, n = 52, only urinary) and domestic sewage (DS, n = 33) E. coli isolates was determined by the microdilution method, according to the European Committee on Antimicrobial Susceptibility Testing. The presence of AMEs among E. coli isolates was determined based on the susceptibility profile to amikacin, gentamicin, kanamycin, and tobramycin, according to Mancini et al. (2019). Results: Overall, 33.3% of the DS isolates and 100% of the CI isolates presented mechanisms of resistance to amikacin, gentamicin, or neomycin. The extended-spectrum beta-lactamase enzymes-producing isolates (23/27, 85%) showed mechanisms of resistance to gentamicin and/or neomycin and resistance to amikacin was simultaneously observed only in CI isolates. All DS isolates were considered wild-type-no AME, while APH (3') (14/52) and AAC (3') (10/52) enzymes were detected among CI isolates, one of which produces APH (3') and AAC (6')-I simultaneously. Conclusion: Resistance to aminoglycosides is present among E. coli isolates in Brazil, but to a lesser extent in environmental isolates. Besides, AMEs are frequent in CI isolates, and surveillance for antimicrobial resistance should be implemented to monitor aminoglycoside-resistant E. coli infections.

Introducción: Escherichia coli se encuentra en diversos ambientes y causa enfermedades humanas. Los aminoglucósidos son antimicrobianos que presentan actividad contra especies gramnegativas. Sin embargo, el uso indiscriminado de estos compuestos ha seleccionado microorganismos resistentes, principalmente debido a la producción de enzimas modificadoras de aminoglucósidos (AME). Material y métodos: La concentración mínima inhibitoria de aminoglucósidos frente a aislados de E.coli clínicos (CI, n = 52) y de aguas residuales sanitarias (DS, n = 33) se determinó mediante el método de microdilución, según la European Committee on Antimicrobial Susceptibility Testing. La presencia de AME se determinó con base en el perfil de susceptibilidad a amikacina, gentamicina, kanamicina y tobra-micina, según Mancini et al. (2019). Resultados: 33,3% de los aislados de DS y 100% de los CI presentaron resistencia a amikacina, gentamicina o neomicina. Los aislados productores de enzimas betalactamasas de espectro extendido (23/27, 85%) mostraron resistencia a gentamicina y/o neomicina y la resistencia a amikacina se observó simultáneamente solo en CI. Todos los aislados de DS se consideraron wild type sin AME, mientras que las enzimas APH (3') (14/52) y AAC (3') (10/52) se detectaron entre CI, uno de los cuales produce APH (3') y AAC (6')-I simultáneamente. Conclusión: La resistencia a los aminoglucósidos está presente entre los aislados de E. coli en Brasil, pero en menor grado en los aislados ambientales. Se debe implementar la vigilancia de la resistencia a los antimicrobianos para monitorear las infecciones por E. coli resistentes a los aminoglucósidos.

SUMÁRIO Introdução: Escherichia coli é encontrada em vários ambientes e causa doenças em humanos. Os aminoglicosídeos são antimicrobianos que exibem atividade contra espécies Gram-negativas. No entanto, o uso indiscriminado desses compostos tem selecionado microrganismos resistentes, principalmente devido à produção de enzimas modificadoras de aminoglicosídeos (EMA). Material e métodos: A concentração inibitória mínima de aminoglicosídeos contra isolados de E. coli recuperadas de amostras clínicas (IC, n=52) e de águas residuais sanitárias (AR, n=33) foi determinada pelo método de microdiluição, de acordo com o European Committee on Antimicrobial Susceptibility Testing. A presença de EMA foi determinada com base no perfil de suscetibilidade à amicacina, gentamicina, canamicina e tobramicina, de acordo com Mancini et al. (2019). Resultados: 33,3% dos ARS e 100% dos ICs apresentaram resistência à amicacina, gentamicina ou neomicina. Os isolados produtores de enzima beta-lactamase de espectro estendido (23/27, 85%) mostraram resistência à gentamicina e/ou neomicina e resistência à amicacina foi observada simultaneamente apenas em um IC. Todos os ARs foram considerados de tipo selvagem sem EMA, enquanto as enzimas APH (3') (14/52) e AAC (3') (10/52) foram detectadas entre os ICs, um dos quais produz APH (3') e AAC (6')-I simultaneamente. Conclusão: A resistência aos aminoglicosídeos está presente entre isolados clínicos de E. coli no Brasil, mas em menor grau em isolados ambientais. Assim a vigilância da resistência antimicrobiana deve ser implementada para monitorar infecções por E. coli resistentes aos aminoglicosídeos.

Repeated dose of meloxicam induces genotoxicity and histopathological changes in cardiac tissue of mice.

Meloxicam is the non-steroidal anti-inflammatory drug most used in small animals; however, studies on genotoxicity, oxidative stress, and histopathologic alterations in cardiac tissue are limited, especially at therapeutical doses used in these animals. This study evaluated the toxic effects caused by the treatment involving repeated low at higher doses of meloxicam in mice, by genotoxicity, oxidative stress, and histopathological parameters. Mice (CF1, male) received, by gavage, meloxicam at the therapeutic dose indicated for small animals (0.1 mg/kg) and at higher doses (0.5 and 1 mg/kg) for 28 days. Later, they were euthanized for blood and organ analysis. Oxidative stress was analyzed by the plasma ferric reduction capacity (FRAP) and catalase, and genotoxicity, by the comet assay and the micronucleus test. Heart, liver, lung, and kidney tissues were analyzed by the histology, and stomach and duodenum were analyzed with a magnifying glass. The relative weight of organs did not present significant alterations. However, congestion of duodenum vessels was observed at the three tested doses and caused hyperemia of stomach mucosa at 1 mg/kg. In the heart histology there was a reduction in the number of cardiomyocytes, accompanied by an increase in cell diameter (possible cell hypertrophy) dose-dependent. The highest tested dose of meloxicam also increased the DNA damage index, without alterations in the micronucleus test. Meloxicam did not affect the catalase activity but increased the FRAP (1 mg/kg). Meloxicam at the dose prescribed for small animals could potentially cause cardiac histopathologic alterations and genotoxic effects.

Risk factors for frontal fibrosing alopecia: A case-control study in a multiracial population.

BACKGROUND: Frontal fibrosing alopecia (FFA) is a chronic cicatricial alopecia with unknown etiology and a worldwide rising incidence. OBJECTIVE: The objective of this study was to evaluate the association of FFA with demographic and exposure factors in a Brazilian multiracial population. METHODS: A multicenter case-control study was conducted in 11 referral centers throughout Brazil. The study was a case-control study that prospectively recruited 902 participants (451 patients with FFA and 451 sex-matched control individuals). Study participants completed a thorough questionnaire comprising variables grouped as baseline demographics, environmental exposure, diet, hormonal factors, allergies, and hair and skin care. RESULTS: When adjusted by sex, age, menopause, and skin color, FFA was associated with hair straightening with formalin (odds ratio [OR], 3.18), use of ordinary (nondermatologic) facial soap (OR, 2.09) and facial moisturizer (OR, 1.99), thyroid disorders (OR, 1.69), and rosacea (OR, 2.08). Smokers (OR, 0.33) and users of antiresidue/clarifying shampoo (OR, 0.35) presented a negative association with FFA. There was no association with the use of sunscreen. LIMITATIONS: Recall bias. CONCLUSIONS: The association with moisturizers, ordinary facial soap, and hair straightening with formalin and the negative association with antiresidue/clarifying shampoo reinforce the possibility of an exogenous particle triggering FFA.

Linfoma intestinal em um cão da raça boxer: relato de caso / Intestinal lymphoma in a Boxer breed dog: case report / Linfoma intestinal en un perro Bóxer: relato de caso

O linfoma intestinal é definido pela presença da neoplasia no trato gastrintestinal e/ou nos linfonodos mesentéricos, sendo o segundo tipo de linfoma mais comum em cães. Os principais sinais clínicos incluem vômito, diarreia, anorexia e perda de peso. O diagnóstico definitivo é realizado por citologia ou histopatologia do órgão lesionado. O protocolo terapêutico mais indicado é a poliquimioterapia. No presente trabalho relata-se o caso de um canino da raça boxer de cinco anos de idade, com histórico de hematoquezia, apatia havia 7 dias e anorexia e perda de peso persistente havia mais de 20 dias. A ultrassonografia abdominal identificou líquido livre e presença de uma massa atingindo parte do intestino. Realizaram-se laparotomia exploratória e coleta de material para o exame histopatológico, confirmando o diagnóstico de linfoma intestinal. Entretanto, durante o procedimento, o paciente veio a óbito.(AU)

Intestinal lymphoma is the second most common form of lymphoma in dogs. It is characterized by the presence of neoplastic lymphocytes in the gastrointestinal tract or mesenteric lymph nodes. The chief clinical signs are vomiting, diarrhea, anorexia and weight loss. The diagnosis is made through fine needle aspiration and cytology, or by tissue biopsy and histopathology, and chemotherapy is the treatment of choice. We report a case of intestinal lymphoma in a five years old Boxer, presented with history of hematochezia and lethargy of 7 days duration, and 20 days of anorexia and persistent weight loss. Abdominal ultrasonography revealed free fluid in the abdominal cavity, and an intestinal mass. Exploratory laparotomy was performed and a tissue biopsy was performed. Histopathology confirmed the diagnosis of intestinal lymphoma, but the patient died during the surgical procedure.(AU)

El linfoma intestinal es el segundo tipo de neoplasia más frecuente en perros y se caracteriza por la presencia de este tumor en el sistema gastrointestinal o linfonódulos mesentéricos. Los signos clínicos más importantes son vómitos, diarrea, anorexia y pérdida de peso. El diagnóstico definitivo se realiza por cito o histopatología. El protocolo terapéutico más indicado es la poliquimioterapia. En este trabajo se relata el caso de un perro Bóxer de cinco anos, que presentaba histórico de hematoquecia, apatía de una semana y anorexia y pérdida de peso persistente durante más de 20 días. La ecografía abdominal permitió identificar líquido libre y una masa que tomaba parte del intestino. Se realizó una laparotomía exploratoria, obteniéndose material para histopatología, examen este que confirmó la presencia de un linfoma intestinal. No obstante, el paciente murió durante la cirugía.(AU)

Linfoma intestinal em um cão da raça boxer: relato de caso / Intestinal lymphoma in a Boxer breed dog: case report / Linfoma intestinal en un perro Bóxer: relato de caso

O linfoma intestinal é definido pela presença da neoplasia no trato gastrintestinal e/ou nos linfonodos mesentéricos, sendo o segundo tipo de linfoma mais comum em cães. Os principais sinais clínicos incluem vômito, diarreia, anorexia e perda de peso. O diagnóstico definitivo é realizado por citologia ou histopatologia do órgão lesionado. O protocolo terapêutico mais indicado é a poliquimioterapia. No presente trabalho relata-se o caso de um canino da raça boxer de cinco anos de idade, com histórico de hematoquezia, apatia havia 7 dias e anorexia e perda de peso persistente havia mais de 20 dias. A ultrassonografia abdominal identificou líquido livre e presença de uma massa atingindo parte do intestino. Realizaram-se laparotomia exploratória e coleta de material para o exame histopatológico, confirmando o diagnóstico de linfoma intestinal. Entretanto, durante o procedimento, o paciente veio a óbito.

Intestinal lymphoma is the second most common form of lymphoma in dogs. It is characterized by the presence of neoplastic lymphocytes in the gastrointestinal tract or mesenteric lymph nodes. The chief clinical signs are vomiting, diarrhea, anorexia and weight loss. The diagnosis is made through fine needle aspiration and cytology, or by tissue biopsy and histopathology, and chemotherapy is the treatment of choice. We report a case of intestinal lymphoma in a five years old Boxer, presented with history of hematochezia and lethargy of 7 days duration, and 20 days of anorexia and persistent weight loss. Abdominal ultrasonography revealed free fluid in the abdominal cavity, and an intestinal mass. Exploratory laparotomy was performed and a tissue biopsy was performed. Histopathology confirmed the diagnosis of intestinal lymphoma, but the patient died during the surgical procedure.

El linfoma intestinal es el segundo tipo de neoplasia más frecuente en perros y se caracteriza por la presencia de este tumor en el sistema gastrointestinal o linfonódulos mesentéricos. Los signos clínicos más importantes son vómitos, diarrea, anorexia y pérdida de peso. El diagnóstico definitivo se realiza por cito o histopatología. El protocolo terapéutico más indicado es la poliquimioterapia. En este trabajo se relata el caso de un perro Bóxer de cinco anos, que presentaba histórico de hematoquecia, apatía de una semana y anorexia y pérdida de peso persistente durante más de 20 días. La ecografía abdominal permitió identificar líquido libre y una masa que tomaba parte del intestino. Se realizó una laparotomía exploratoria, obteniéndose material para histopatología, examen este que confirmó la presencia de un linfoma intestinal. No obstante, el paciente murió durante la cirugía.

Trichoscopy of Steroid-Induced Atrophy.

Intralesional corticosteroid (IL-CS) injections have been used to treat a variety of dermatological and nondermatological diseases. Although an important therapeutic tool in dermatology, a number of local side effects, including skin atrophy, have been reported following IL-CS injections. We recently noticed that a subset of patients with steroid-induced atrophy presented with ivory-colored areas under trichoscopy. We performed a retrospective analysis of trichoscopic images and medical records from patients presenting ivory-colored areas associated with atrophic scalp lesions. In this paper, we associate this feature with the presence of steroid deposits in the dermis and report additional trichoscopic features of steroid-induced atrophy on the scalp, such as prominent blood vessels and visualization of hair bulbs.

Draft genome sequence of Bacillus thuringiensis 147, a Brazilian strain with high insecticidal activity.

Bacillus thuringiensis is a ubiquitous Gram-positive and sporulating bacterium. Its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. We report here the draft genome sequence of B. thuringiensis147, a strain isolated from Brazil and with high insecticidal activity. The assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). We identified 12 coding regions, located in two plasmids, which encode insecticidal proteins.

Draft genome sequence of Bacillus thuringiensis 147, a Brazilian strain with high insecticidal activity

Bacillus thuringiensisis a ubiquitous Gram-positive and sporulating bacterium. Its crystals and secreted toxins are useful tools against larvae of diverse insect orders and, as a consequence, an alternative to recalcitrant chemical insecticides. We report here the draft genome sequence ofB. thuringiensis147, a strain isolated from Brazil and with high insecticidal activity. The assembled genome contained 6,167,994 bp and was distributed in seven replicons (a chromosome and 6 plasmids). We identified 12 coding regions, located in two plasmids, which encode insecticidal proteins.

Saberes sobre saúde entre pessoas vivendo em situação de rua / Knowledge about health among people living on streets

Esta pesquisa teve como objetivo identificar os saberes da população vivendo em situação de rua sobre a saúde. As informações foram colhidas por meio de entrevistas individuaisfeitas com 27 sujeitos moradores de rua, frequentadores de uma instituição filantrópica situada em Belém – Pará. Os dados foram analisados por meio da técnica de análise de conteúdo temática. Os resultados mostraram que os moradores de rua consideram saúde como sendo um estado ou situação que, na maioria das vezes, não condiz com seu estado atual e que comumente configura-se para eles como uma realidade quase inalcançável. Avaliam que a saúde é resultante da somatória das condições de alimentação, habitação, educação, renda, meio ambiente, trabalho, transporte, emprego, lazer e liberdade. Conclui-se que os problemas frequentes no contexto da saúde, enfrentados por essa população, têm variadas causas,entretanto merece destaque o uso de drogas que foi citado pelos sujeitos como principal motivo para a vivência na rua e consequentemente a perda do vínculo familiar e o desemprego. Adependência das drogas é um fator considerado na auto avaliação do estado de saúde. Assim, no cotidiano das ruas, os que nela vivem constroem seus saberes sobre a saúde, baseadosprincipalmente naquilo que a afeta. (AU)

This research aimed to identify the knowledge of population living on streets about health. Data were collected through individual interviews made with 27 homeless subjects, frequenters of a philanthropic institution located in Belém – Pará. Data were analyzed using the technique of thematic content analysis. The results showed homeless consider health as a state or situation that, in most cases, is not consistent with its current state, and that usually sets up for them as an almost unreachable reality. They estimate that health is the sum result conditions of food, housing, education, income, environment, labor, transportation, employment, leisure and freedom. We conclude that common problems in the context of health, faced by this population, have varied causes, however noteworthy, drug use, which was cited by the subjects as the main reason for living on streets, and consequently the loss of family bond and unemployment. Drug addiction is a factor considered in the self-assessment of health status. Thus, on streets everyday, people who live in building their knowledge about health based mostly on what the affects. (AU)

Saberes sobre saúde entre pessoas vivendo em situação de rua / Knowledge about health among people living on streets

Esta pesquisa teve como objetivo identificar os saberes da população vivendo em situação de rua sobre a saúde. As informações foram colhidas por meio de entrevistas individuaisfeitas com 27 sujeitos moradores de rua, frequentadores de uma instituição filantrópica situada em Belém – Pará. Os dados foram analisados por meio da técnica de análise de conteúdo temática. Os resultados mostraram que os moradores de rua consideram saúde como sendo um estado ou situação que, na maioria das vezes, não condiz com seu estado atual e que comumente configura-se para eles como uma realidade quase inalcançável. Avaliam que a saúde é resultante da somatória das condições de alimentação, habitação, educação, renda, meio ambiente, trabalho, transporte, emprego, lazer e liberdade. Conclui-se que os problemas frequentes no contexto da saúde, enfrentados por essa população, têm variadas causas,entretanto merece destaque o uso de drogas que foi citado pelos sujeitos como principal motivo para a vivência na rua e consequentemente a perda do vínculo familiar e o desemprego. Adependência das drogas é um fator considerado na auto avaliação do estado de saúde. Assim, no cotidiano das ruas, os que nela vivem constroem seus saberes sobre a saúde, baseadosprincipalmente naquilo que a afeta.

This research aimed to identify the knowledge of population living on streets about health. Data were collected through individual interviews made with 27 homeless subjects, frequenters of a philanthropic institution located in Belém – Pará. Data were analyzed using the technique of thematic content analysis. The results showed homeless consider health as a state or situation that, in most cases, is not consistent with its current state, and that usually sets up for them as an almost unreachable reality. They estimate that health is the sum result conditions of food, housing, education, income, environment, labor, transportation, employment, leisure and freedom. We conclude that common problems in the context of health, faced by this population, have varied causes, however noteworthy, drug use, which was cited by the subjects as the main reason for living on streets, and consequently the loss of family bond and unemployment. Drug addiction is a factor considered in the self-assessment of health status. Thus, on streets everyday, people who live in building their knowledge about health based mostly on what the affects.

Consensus on melanonychia nail plate dermoscopy.

This statement, focused on melanonychia and nail plate dermoscopy, is intended to guide medical professionals working with melanonychia and to assist choosing appropriate management for melanonychia patients. The International Study Group on Melanonychia was founded in 2007 and currently has 30 members, including nail experts and dermatopathologists with special expertise in nails. The need for common definitions of nail plate dermoscopy was addressed during the Second Meeting of this Group held in February 2008. Prior to this meeting and to date (2010) there have been no evidence-based guidelines on the use of dermoscopy in the management of nail pigmentation.

Amputações por pé diabético e fatores sociais: implicações para cuidados preventivos de enfermagem / Amputations for diabetic foot and social factors: implications for nursing preventive care

Muitos estudos têm apontado fatores de risco associados à ocorrência de amputação por pé diabético, no entanto, a análise dos fatores sociais tem sido pouco estudado. O objetivo deste estudo foi o de verificar a ocorrência de amputações em portadores de pé diabético segundo fatores sociais e suas respectivas implicações para os cuidados preventivos de enfermagem. Estudo epidemiológico retrospectivo, do tipo caso-controle. A amostra foi composta por 64 portadores de pé diabético internados na clínica vascular de um hospital público do Recife. Para cada paciente submetido à amputação coube o exame de um controle. Os resultados mostraram a existência de associação entre amputação e anos de estudo inferior a cinco anos, renda familiar de até um salário mínimo e número de 3 a mais pessoas residindo no domicílio. O conhecimento destas associações requer de gestores públicos e dos profissionais da atenção básica o planejamento de estratégias diferenciadas de abordagem e acompanhamento da população em risco.

O'Brien's actinic granuloma: an unusually extensive presentation.

O'Brien's actinic granuloma is a rare skin disease. Controversy continues over whether it should be considered a specific condition or a form of granuloma annulare located in sun-exposed areas. Its pathogenesis is unknown; however, the most widely accepted hypothesis suggests that solar radiation is the triggering factor. This paper describes the case of a 78-year old, fair-skinned male, who presented with a 10-year history of an infiltrate of annular erythematous papules on his forehead and left malar region. The diagnosis of O'Brien actinic granuloma was established from histopathology, since the clinical condition of the patient was extensive, unlike cases reported in the literature.

Granuloma actínico de O'Brien: apresentação clínica exuberante / O'Brien's actinic granuloma: an unusually extensive presentation

O granuloma actínico de O'Brien é uma doença cutânea rara. Há muitas controvérsias na sua caracterização, podendo representar uma entidade nosológica distinta ou uma forma de granuloma anular em áreas expostas. A patogênese é desconhecida; no entanto, acredita-se que a radiação solar seja um fator desencadeante. Os autores relatam o caso de um paciente de 78 anos, branco, que apre sentava há 10 anos pápulas eritematosas e infiltradas na fronte e hemiface à esquerda. O diagnóstico de granuloma actínico de O'Brien foi estabelecido a partir do exame histopatológico, uma vez que o quadro clínico exuberante apresentado pelo paciente diferia do de casos anteriormente observados.

O'Brien's actinic granuloma is a rare skin disease. Controversy continues over whether it should be considered a specific condition or a form of granuloma annulare located in sun-exposed areas. Its pathogenesis is unknown; however, the most widely accepted hypothesis suggests that solar radiation is the triggering factor. This paper describes the case of a 78-year old, fair-skinned male, who presented with a 10-year history of an infiltrate of annular erythematous papules on his forehead and left malar region. The diagnosis of O'Brien actinic granuloma was established from histopathology, since the clinical condition of the patient was extensive, unlike cases reported in the literature.

[Dermoscopy in nail psoriasis]. / Aspectos dermatoscópicos na psoríase ungueal.

The authors report on their experience with the use of dermoscopy in nail psoriasis and describe their findings with this diagnostic tool.

Aspectos dermatoscópicos na psoríase ungueal / Dermoscopy in nail psoriasis

Os autores relatam sua experiência no uso da dermatoscopia na psoríase ungueal e descrevem os achados dessa ferramenta diagnóstica.

The authors report on their experience with the use of dermoscopy in nail psoriasis and describe their findings with this diagnostic tool.

Frequency and main sites of allergic contact dermatitis caused by nail varnish.

BACKGROUND: Liquid nail varnish has been used since 1919, and allergic contact dermatitis (ACD) has been recognized for at least 80 years, but it is difficult for nonspecialists to identify this condition. OBJECTIVES: (1) To verify the frequency of ACD from nail varnish in patients with a presumptive diagnosis of contact dermatitis seen at an outpatient clinic, (2) to characterize the groups studied according to site of skin disorder, and (3) to determine the main sensitizer related to varnish. METHODS: Patients with a final diagnosis of ACD caused by nail varnish were assessed by means of retrospective analysis of medical charts and protocols used in the clinic from January 1996 to December 2006. Patch tests with the Brazilian standard series and a complementary series were applied to all patients. RESULTS: Diagnosis of ACD from nail varnish was made in 8% of cases (157 of 1,971). The most affected sites were the face and neck; however, involvement of some uncommon areas, such as periungual and perianal regions, was also observed. CONCLUSIONS: ACD from nail polishes is a common event and recognition of the condition must be improved. Toluenesulfonamide formaldehyde resin (TSFR) was the most common allergen in the group studied.

Você conhece esta síndrome? / Do you know this syndrome?

Paquidermoperiostose é genodermatose autossômica dominante rara, que afeta pele e ossos, porém sua prevalência e incidência permanecem desconhecidas. Ocorre predominantemente em homens, que também mostram fenótipo mais grave da doença. Descrevese o caso de paciente do sexo masculino, de 60 anos.

Pachydermoperiostosis is a rare autosomal dominant genodermatosis characterized by bone and cutaneous involvement, even though its prevalence and incidence are ignored. It occurs predominantly in men, which present a more serious clinical presentation. We report a case of a 60-year-old man with this disease.

Histological study of the liver and biochemistry of the blood of Wistar rats following ligature of right hepatic duct.

PURPOSE: To observe the histological alterations in the liver and biochemistry in the blood that can happen in Wistar rat, after the ligature of right hepatic duct. METHODS: In this study were used rats (n=46) of Wistar pedigree. The animal groups (n=46) were distributed in 6 experimented sub-groups (n=6). It was held a ligature surgery of the right hepatic duct and euthanasia in 7, 14, 21, 28, 60 and 90 days and the biochemistry control group (n=10), that animals had 2ml of their blood taken by cardiac puncture for biochemistry study with value analyses of bilirubins, transaminasis, lactic desidrogenasis, alkaline phophatase and gamma-glutamil-transferase. Given the expected time of each group, the animals were submitted to anesthesia procedure and cavity re-opening, being held intra-cardiac puncture and with 2ml blood collected for biochemistry analyses. It was proceeded the liver resection, being the liver put in formol solution to 10% for a period of 24 hours and taken to the histology. RESULTS: It was not possible to identify results that express significant differences as the existence of alterations histological and biochemical between the different groups. CONCLUSION: At the end of the study, it was not possible to identify histological and biochemical alterations that express significant differences between livers of the animals from the right linked hepatic duct and the animals of the control group.