Osteotomia periférica no tratamento de cisto odontogênico glandular / Peripheral osteotomy in the treatment of glandular odontogenic cyst / Osteotomía periférica en el tratamiento del quiste odontogénico glandular

O objetivo deste artigo é relatar um caso de COG em sínfise mandibular, tratado com enucleação e osteotomia periférica. Relato de Caso: Paciente do sexo feminino, 58 anos de idade, foi encaminhada para avaliação de uma lesão mandibular, apresentando-se assintomática no momento da consulta. Foi observado um leve aumento de volume vestibular na região da sínfise mandibular, sem sensibilidade ao toque. Na tomografia foi observada imagem hipodensa, multiloculada, bem delimitada, na região de sínfise, próxima as raízes dentárias e a basilar mandibular, além de um dente incluso intralesional. Foi realizada biópsia incisional e exame histopatológico, através do qual foi estabelecido o diagnóstico de COG. Como forma de tratamento, o paciente foi submetido a enucleação com curetagem de toda lesão e a osteotomia periférica, além da remoção do dente incluso. Após um ano de acompanhamento, a paciente encontra-se livre de recorrências. Conclusão: Por fim, este caso destaca a importância de um tratamento eficaz de COG com a osteotomia periférica, considerando o tamanho da lesão, suas características e sua localização, a fim de reduzir suas chances de recidiva... (AU)

The aim of this article is to report a case of COG in mandibular symphysis, treated with enucleation and peripheral osteotomy. Case Report: A 58-yearold female patient was referred for evaluation of a mandibular lesion, instrument was asymptomatic at the time of consultation. An increase in vestibular volume was observed in the region of the mandibular symphysis, without sensitivity to touch. The tomography showed a hypodense, multiloculated, well-delimited image in the symphysis region, close to tooth roots and a mandibular basilar, in addition to an intralesional impacted tooth. An incisional biopsy and histopathological examination were performed, through which the diagnosis of COG was established. As a form of treatment, the patient underwent enucleation with curettage of the entire lesion and peripheral osteotomy, in addition to removal of the impacted tooth. After a year of follow-up, the patient is free from recurrences. Conclusion: Finally, this case highlights the importance of an effective treatment of COG with peripheral osteotomy, considering the size of the lesion, its characteristics and its location, in order to reduce its chances of recurrence... (AU)

El objetivo de este artículo es reportar un caso de COG en la sínfisis mandibular, tratado con enucleación y osteotomía periférica. Caso Clínico: Paciente femenino, de 58 años de edad, fue remitida para valoración de lesión mandibular, presentándose asintomática al momento de la consulta. Se observa ligero aumento de volumen vestibular en la región de la sínfisis mandibular, sin sensibilidad al tacto. En la tomografía se observó una imagen hipodensa, multiloculada, bien delimitada en la región de la sínfisis, próxima a las raíces dentarias y la base de la mandíbula, además de un diente intralesional. Se realizó biopsia incisional y examen histopatológico, a través del cual se estableció el diagnóstico de GOC. Como forma de tratamiento, el paciente fue sometido a enucleación con curetaje de toda la lesión y osteotomía periférica, además de extracción del diente impactado. Después de un año de seguimiento, un paciente está libre de recurrencias. Conclusión: Finalmente, este caso destaca la importancia de un tratamiento efectivo de la GOC con osteotomía periférica, considerando el tamaño de la lesión, sus características y su ubicación, para reducir sus posibilidades de recurrencia... (AU)

Potential of tellurite resistance in heterotrophic bacteria from mining environments.

Untreated mining wastes and improper disposal of high-tech devices generate an environmental increase of bioavailable metalloids, exerting stress on autochthonous microbial populations. Tellurium is a metalloid, an element with raising economic importance; nevertheless, its interaction with living organisms is not yet fully understood. Here we characterized aerobic heterotrophic bacteria, isolated from high metal-content mining residues, able to resist/reduce tellurite into tellurium structures and to determine the presence of confirmed tellurite resistance genetic determinants in resistant strains. We identified over 50 tellurite-resistant strains, among 144 isolates, eight strains reduced tellurite to tellurium at different rates, with the concomitant production of tellurium deposits. Most tellurite resistance genes were found in strains from Bacillales, with the prevalence of genes of the ter operon. This work demonstrated that bacterial isolates, from environments with a persistent selective pressure, are potential candidates for uncovering strategies for tellurite resistance and/or production of valuable Te-containing materials.

Impact of Tellurite on the Metabolism of Paenibacillus pabuli AL109b With Flagellin Production Explaining High Reduction Capacity.

Tellurium (Te) is a metalloid with scarce and scattered abundance but with an increased interest in human activity for its uses in emerging technologies. As is seen for other metals and metalloids, the result of mining activity and improper disposal of high-tech devices will lead to niches with increased abundance of Te. This metalloid will be more available to bacteria and represent an increasing selective pressure. This environmental problem may constitute an opportunity to search for microorganisms with genetic and molecular mechanisms of microbial resistance to Te toxic anions. Organisms from Te-contaminated niches could provide tools for Te remediation and fabrication of Te-containing structures with added value. The objective of this study was to determine the ability of a high metal-resistant Paenibacillus pabuli strain ALJ109b, isolated from high metal content mining residues, to reduce tellurite ion, and to evaluate the formation of metallic tellurium by cellular reduction, isolate the protein responsible, and determine the metabolic response to tellurite during growth. P. pabuli ALJ109b demonstrated to be resistant to Te (IV) at concentrations higher than reported for its genus. It can efficiently remove soluble Te (IV) from solution, over 20% in 8 h of growth, and reduce it to elemental Te, forming monodisperse nanostructures, verified by scattering electron microscopy. Cultivation of P. pabuli ALJ109b in the presence of Te (IV) affected the general protein expression pattern, and hence the metabolism, as demonstrated by high-throughput proteomic analysis. The Te (IV)-induced metabolic shift is characterized by an activation of ROS response. Flagellin from P. pabuli ALJ109b demonstrates high Te (0) forming activity in neutral to basic conditions in a range of temperatures from 20°C to 37°C. In conclusion, the first metabolic characterization of a strain of P. pabuli response to Te (IV) reveals a highly resistant strain with a unique Te (IV) proteomic response. This strain, and its flagellin, display, all the features of potential tools for Te nanoparticle production.

Vivenciando as desordens na prática do cuidado do enfermeiro obstetra: o olhar complexo ao fenômeno / Living disorders in the practice of obstetric nurse care: the complex look at the phenomenon / Vivenciando los desordes en la práctica del cuidado del enfermero obstetra: la mirada complejo al fenómeno

Objetivo: Compreender as desordens vivenciadas pelo enfermeiro em sua prática do cuidado no parto, à luz da Complexidade. Método: Estudo qualitativo com delineamento da Teoria Fundamentada nos Dados como referencial metodológico e a Teoria da Complexidade como suporte teórico. Foram entrevistados 31 participantes de maternidades do Rio Grande do Norte, Brasil organizados em três grupos amostrais de enfermeiros, gestores de saúde e médicos. Resultados: Os enfermeiros obstetras vivenciam desordens em relação à sua autonomia, ao poder do médico e a violência obstétrica no processo de parto, à falta de apoio da gestão de saúde e gestão das maternidades, à falta de organização da rede e, como consequência, vivenciam sentimentos negativos na atuação. Conclusão: Essas deverão ser superadas como possibilidade de mudança no modelo de atenção do enfermeiro obstetra

Objective: Understand the disorders experienced by nurses in their practice of childbirth care, in the light of Complexity. We used the Grounded Theory as a methodological reference and the Complexity Theory as theoretical support. Method: Qualitative study. We used the Grounded Theory as a methodological reference and the Complexity Theory as theoretical support. We interviewed 31 participants in three sample groups of nurses, health managers and physicians. Results: Obstetric nurses experience disorders in relation to their autonomy, the power of the doctor and obstetric violence in the birthing process, the lack of support from the health management and maternity management, the lack of organization of the network and, as a consequence, they experience feelings performance. Conclusion: These should be overcome as a possibility of change in the care model of the obstetrician nurse

Objetivo: Comprender los desórdenes vivenciados por el enfermero en su práctica del cuidado en el parto, a la luz de la complejidad. Se utilizaron la Teoría Fundamentada en los Datos como referencial metodológico y la Teoría de la Complejidad como soporte teórico. Método: Estudio de naturaleza cualitativa. Se utilizaron la Teoría Fundamentada en los Datos como referencial metodológico y la Teoría de la Complejidad como soporte teórico. Se entrevistó a 31 participantes organizados en tres grupos muestrales de enfermeros, gestores de salud y médicos. Resultado: Los enfermeros obstetras experimentan desordenes en relación a su autonomía, al poder del médico y la violencia obstétrica en el proceso de parto, a la falta de apoyo de la gestión de salud y gestión de las maternidades, a la falta de organización de la red y, como consecuencia, experimentan sentimientos negativos en la actuación. Conclusión: Estas deben ser superadas como posibilidad de cambio en el modelo de atención del enfermero obstetra

Insights into the earthworm gut multi-kingdom microbial communities.

Earthworms are widely known to impact soil health, having a key role in nutrient cycling and are often referred to as soil engineers. They are vital for soil microbial assemblages particularly through their feeding and burrowing activity in soil. Earthworms feed on soil organic matter and litter, and the resulting casts alter the soil microbial community. However, the gut microbiome of earthworms remains less known. In this study, we used amplicon sequencing of the 16S rRNA gene for bacteria and 18S rRNA gene for eukaryotes to assess the gut community assemblages of earthworm species within three genera Aporrectodea, Allolobophora and Lumbricus that represent different life forms sharing the same habitat. The objective was to compare the gut microbiome profiles of eukaryotic and prokaryotic organisms to assess significance of earthworm life forms, and to explore the cross kingdom networks in an attempt to identify keystone species. We found a high eukaryotic diversity with a dominance of the SAR supergroup along with fungi and metazoan in the earthworm gut. The bacterial community were dominated by members of Proteobacteria, Acidobacteria, Actinobacteria, Firmicutes, and Verrucomicrobia. The eukaryotic and prokaryotic communities showed similar differences in alpha diversity, being lowest in Lumbricus herculeus. The beta diversity showed earthworm species as a key factor in shaping gut microbiomes with L. herculeus harboring distinct microbiomes compared to species of Aporrectodea caliginosa, A. longa, A. tuberculata and Allolobophora chlorotica. Cross kingdom networks showed high interactions between several protist and bacterial OTUs. In conclusion, this study suggested that the community assemblages of gut microbiomes were shaped by earthworm species and life form, and such assemblage consists of cross kingdom interactions among eukaryotes and prokaryotes.

Tailings microbial community profile and prediction of its functionality in basins of tungsten mine.

In a circular economy concept, where more than 300 million tons of mining and quarrying wastes are produced annually, those are valuable resources, supplying metals that are extracted today by other processes, if innovative methods and processes for efficient extraction of these elements are applied. This work aims to assess microbiological and chemical spatial distribution within two tailing basins from a tungsten mine, using a MiSeq approach targeting the 16S rRNA gene, to relate microbial composition and function with chemical variability, thus, providing information to enhance the efficiency of the exploitation of these secondary sources. The tailings sediments core microbiome comprised members of family Anaerolineacea and genera Acinetobacter, Bacillus, Cellulomonas, Pseudomonas, Streptococcus and Rothia, despite marked differences in tailings physicochemical properties. The higher contents of Al and K shaped the community of Basin 1, while As-S-Fe contents were correlated with the microbiome composition of Basin 2. The predicted metabolic functions of the microbiome were rich in genes related to metabolism pathways and environmental information processing pathways. An in-depth understanding of the tailings microbiome and its metabolic capabilities can provide a direction for the management of tailings disposal sites and maximize their potential as secondary resources.

Órteses de EVA no tratamento para pé torto congênito em recém-nascidos / EVA brace for treatment of congenital clubfoot in newborns

Resumo Introdução: Pé torto congênito (PTC) é uma deformidade ortopédica caracterizada por uma displasia congênita das estruturas musculoesqueléticas como músculos, tendões, ligamentos, estruturas osteoarticulares e neurovasculares, de origem multifatorial e causa idiopática. Objetivo Oferecer intervenção precoce no tratamento de pé torto congênito de recém-nascidos e apresentar o uso de Etil Vinil Acetato (EVA) como um material para produção de órteses ainda no período neonatal. Método Foi realizada intervenção em recém-nascidos de idade gestacional >37 semanas, de ambos os sexos, diagnosticados com pé torto congênito flexível. As órteses foram desenvolvidas com EVA de espessura 4 mm, velcro e cola quente, utilizando-se prancha de cabelo e tesoura para confecção. Resultados Foram incluídos 15 neonatos no estudo, com PTC bilateral. Para cada confecção de órtese foi usado em média 20 cm2 de EVA e nenhum recém-nascido evoluiu com úlcera de pressão. As órteses foram mantidas por 18 horas inicialmente e nenhuma família apresentou qualquer queixa ou dificuldade em manipular o material em casa, relatando integral satisfação. Aos 60 dias de tratamento, todos os bebês em acompanhamento alcançaram a pontuação 0 pela escala de Pirani, entretanto, por não apresentarem simetria dos pés, foram acompanhados por mais 30 dias. Na análise multivariada, observaram-se diferenças entre as avaliações (p = 0,001), e na comparação de pares, apenas a pontuação dos 30 com 60 dias, foram semelhantes (p = 0,45). Conclusão A rápida intervenção, realizada por profissional habilitado, utilizando-se órteses confeccionadas com EVA, mostrou-se eficaz para o tratamento conservador do PTC em recém-nascidos.

Abstract Introduction Congenital clubfoot (CC) is an orthopedic deformity characterized by congenital dysplasia of musculoskeletal structures such as muscles, tendons, ligaments, osteoarticular and neurovascular structures, of multifactorial and idiopathic origin. Objective Offer early intervention for the treatment of congenital clubfoot in newborns, using ethyl vinyl acetate (EVA) to produce braces in the neonatal period. Method Intervention was performed in newborns of both sexes, with gestational age >37 weeks, diagnosed with flexible congenital clubfoot. The braces were manufactured with 4 mm-thick EVA, Velcro and hot glue, using flat irons and scissors. Results Fifteen newborns were included in the study, with bilateral CC and 32 braces. An average of 20 cm2 of EVA was used for each brace and none of the newborns developed pressure sores. Braces were worn initially for 18 hours and no family member expressed any complaint or difficulty in handling the device at home, reporting complete satisfaction. After 60 days of treatment, all the infants in the follow-up achieved a score of 0 on the Pirani scale, but were monitored for a further 30 days because they did not exhibit symmetrical feet. Multivariate analysis revealed differences between assessments (p = 0.001), and in pair comparison, only a score of 30 after 60 days was similar (p = 0.45). Conclusion Rapid intervention, performed by a qualified professional using EVA braces, proved to be effective for conservative treatment of CC in newborns.

[Nutrition and gastronomy in the Principality of Asturias]. / Nutrición y gastronomía en el Principado de Asturias.

INTRODUCTION: Introduction: Asturias is renowned for its beautiful and diverse nature and landscapes and for its rich gastronomic tradition. Objectives: to understand the traditional gastronomic identity of Asturias and its relationship with food and nutritional intake and health indicators of the population. Methods: bibliographic search (PubMed, Google Scholar) with different combinations of keywords. Results: the gastronomic tradition of Asturias is characterized by a great variety of foods and dishes, with emblematic culinary preparations. Different reports and studies agree that it is one of the Spanish Regions with the highest food consumption, except for vegetables and cereals and derivatives. The energy intake is also among the highest, but with the highest rates of diet quality indexes. The high consumption of food and the high energy intake, together with an aged population with a high prevalence of sedentary lifestyles, could explain that the prevalence of obesity in the adult population of Asturias is the highest of all the Spanish Regions, with an increasing incidence of diabetes. Conclusions: it is necessary to implement public health policies aimed at improving food selection and health indicators. Furthermore, the study of the contribution of traditional dishes to the dietary habits, the nutritional intake and the health indicators of the population of Asturias would help in improving food selection.

INTRODUCCIÓN: Introducción: Asturias es reconocida por su riqueza natural y paisajística y por su rica tradición gastronómica. Objetivos: conocer la identidad gastronómica tradicional de Asturias y su relación con los hábitos de consumo alimentario, la ingesta nutricional y los indicadores de salud de la población. Métodos: búsqueda bibliográfica (PubMed, Google Scholar) con distintas combinaciones de las palabras clave. Resultados: Asturias tiene una gastronomía caracterizada por una gran variedad de alimentos y platos, con preparaciones culinarias emblemáticas. Distintos informes y estudios coinciden en que es una de las comunidades autónomas con mayor consumo y gasto per cápita en alimentación, a pesar de lo cual está entre las de menor consumo de verduras, hortalizas y cereales y derivados (excepto el pan). La ingesta energética está también entre las más altas, si bien el perfil calórico y el perfil lipídico muestran los valores más cercanos a las recomendaciones. El elevado consumo de alimentos y la alta ingesta energética, unidos al envejecimiento poblacional y al elevado sedentarismo, podrían explicar que la prevalencia de obesidad en la población adulta de Asturias sea la más alta de todas las comunidades autónomas, con una creciente incidencia de diabetes. Conclusiones: se hace necesaria la implementación de políticas públicas de salud orientadas a la mejora de la selección de alimentos, así como el estudio de la contribución de los platos tradicionales al conjunto de los hábitos alimenticios y de la ingesta de energía y nutrientes de la población asturiana.

Nutrición y gastronomía en el Principado de Asturias / Nutrition and gastronomy in the Principality of Asturias

Introducción: Asturias es reconocida por su riqueza natural y paisajística y por su rica tradición gastronómica. Objetivos: conocer la identidad gastronómica tradicional de Asturias y su relación con los hábitos de consumo alimentario, la ingesta nutricional y los indicadores de salud de la población. Métodos: búsqueda bibliográfica (PubMed, Google Scholar) con distintas combinaciones de las palabras clave. Resultados: Asturias tiene una gastronomía caracterizada por una gran variedad de alimentos y platos, con preparaciones culinarias emblemáticas. Distintos informes y estudios coinciden en que es una de las comunidades autónomas con mayor consumo y gasto per cápita en alimentación, a pesar de lo cual está entre las de menor consumo de verduras, hortalizas y cereales y derivados (excepto el pan). La ingesta energética está también entre las más altas, si bien el perfil calórico y el perfil lipídico muestran los valores más cercanos a las recomendaciones. El elevado consumo de alimentos y la alta ingesta energética, unidos al envejecimiento poblacional y al elevado sedentarismo, podrían explicar que la prevalencia de obesidad en la población adulta de Asturias sea la más alta de todas las comunidades autónomas, con una creciente incidencia de diabetes. Conclusiones: se hace necesaria la implementación de políticas públicas de salud orientadas a la mejora de la selección de alimentos, así como el estudio de la contribución de los platos tradicionales al conjunto de los hábitos alimenticios y de la ingesta de energía y nutrientes de la población asturiana

Introduction: Asturias is renowned for its beautiful and diverse nature and landscapes and for its rich gastronomic tradition. Objectives: to understand the traditional gastronomic identity of Asturias and its relationship with food and nutritional intake and health indicators of the population. Methods: bibliographic search (PubMed, Google Scholar) with different combinations of keywords. Results: the gastronomic tradition of Asturias is characterized by a great variety of foods and dishes, with emblematic culinary preparations. Different reports and studies agree that it is one of the Spanish Regions with the highest food consumption, except for vegetables and cereals and derivatives. The energy intake is also among the highest, but with the highest rates of diet quality indexes. The high consumption of food and the high energy intake, together with an aged population with a high prevalence of sedentary lifestyles, could explain that the prevalence of obesity in the adult population of Asturias is the highest of all the Spanish Regions, with an increasing incidence of diabetes. Conclusions: it is necessary to implement public health policies aimed at improving food selection and health indicators. Furthermore, the study of the contribution of traditional dishes to the dietary habits, the nutritional intake and the health indicators of the population of Asturias would help in improving food selection

Autonomy for obstetric nurse on low-risk childbirth care / Autonomia do enfermeiro obstetra na assistência ao parto de risco habitual

Abstract Objectives: to understand the cultural context presented in hospitals and its relation to the obstetric nurse's autonomous practice on low-risk childbirth care. Methods: ethnographic research performed in three public maternities in Rio Grande do Norte, Brazil. Three managers and twenty-three obstetric nurses participated in this research. Results: distinctive on cultural, organizational and structural aspects of the hospital institution interfering directly with the obstetric nurse's autonomous practice. Among these aspects, professional appreciation on low-risk childbirth care contributes for the nurse's autonomy Conclusions: it was noticed that obstetric nurses undergo different contexts of action, which directly interfere with their autonomy on low-risk childbirth care and their decisionmaking abilities. It is necessary, then, to eradicate the relation of dominance and submission that it is still imposed by medical hegemony.

Resumo Objetivos: compreender o contexto cultural da instituição hospitalar e sua relação com a prática autônoma do enfermeiro obstetra na assistência ao parto de risco habitual. Métodos: pesquisa etnográfica desenvolvida em três maternidades públicas do Estado do Rio Grande do Norte, Brasil, com três gestores e 23 enfermeiros obstetras. Resultados: diferentes aspectos de ordem cultural, organizacional e estrutural da instituição hospitalar interferem diretamente na prática autônoma do enfermeiro obstetra. Dentre estes aspectos, a valorização profissional na assistência ao parto de risco habitual contribui para a autonomia do profissional em questão. Conclusões: constatou-se que o enfermeiro obstetra vivencia distintos contextos de atuação, os quais interferem diretamente na sua autonomia na assistência ao parto de risco habitual e no seu poder de decisão. Faz-se necessário, então, desvencilhar-se das relações de domínio e submissão, ainda impostas pela hegemonia médica.

Percepção de mulheres quanto à prática do aleitamento materno: uma revisão integrativa / Perception of women regarding the practice of breastfeeding: an integrative review / Percepción de la mujer en cuanto a la práctica de la lactancia materna: una revisión integradora

Objetivo: Identificarel conocimiento científico acerca de la percepción de las mujeres con respecto a la lactancia materna. Método: Una revisión integradora para buscar artículos en MEDLINE, Pub / Med, LILACS, BDENF base de datos central de PubMed se realizó: PMCE CINAHL, de 2008 a 2015, se seleccionaron 30 artículos. Resultado: Surgió a partir del análisis las categorías: Beneficios de la lactancia materna; mitos y tabúes que rodean a la lactancia materna; sentimientos contradictorios durante la lactancia, aspectos culturales,la gestión de la lactancia materna y recomendaciones para la práctica. Conclusión:Percepciones de las mujeres sobre la lactancia materna, pueden contribuir a la formulación de recomendaciones con el fin de ayudar a la enfermera en una perspectiva que va más allá de la técnica respetando los significados y prácticas atribuidas a las mujeres que amamantan

Objective: Identify the contribution of the research on the scientific knowledge about the perception of women regarding breastfeeding. Method: An integrative review was carried and the search for articles occurred in MEDLINE, Pub/Med, LILACS, BDENF, PubMed Central: PMCe CINAHL, from 2008 to 2015, containing 30 articles. Results: Emerging from the analysis were the following categories: benefits of breastfeeding, myths and taboos surrounding breastfeeding; contradictory feelings when breastfeeding; cultural aspects and management of breastfeeding. Conclusion: The results indicate aspects about women's perceptions regarding breastfeeding, which may contribute to the development of recommendation sin order to assist the breast feeder in a perspective that goes beyond the technical, respecting the perceptions, meanings and practices attributed to breastfeeding by women

Objetivo: Identificar o conhecimento científico produzido acerca da percepção das mulheres quanto à prática do aleitamento materno.Método:Revisão integrativa com busca dos artigos nas bases de dados MEDLINE, Pub/Med, LILACS, BDENF, PubMed Central: PMC e CINAHL, de 2008 a 2015, sendo selecionados 30 artigos. Resultados: Da análise, emergiram as categorias: vantagens do aleitamento materno; mitos e tabus em torno da amamentação; sentimentos contraditórios ao amamentar; aspectos culturais; manejo da amamentação; e recomendações para a prática. Conclusão: As percepções das mulheres em relação à amamentação podem contribuir para a elaboração de recomendações com vistas a assistir a nutriz numa perspectiva que vai além da técnica, respeitando os significados e práticas atribuídas à amamentação pelas mulheres

Impact of type of delivery on thoracoabdominal mobility of newborns / Impacto do tipo de parto sobre a mobilidade Toracoabdominal de recém-nascidos

INTRODUCTION: In newborns delivered by cesarean section, there is less chest compression and little amount of fluid is drained by gravity, which temporarily reduces thoracoabdominal mobility OBJETIVE: The objective of this study is to evaluate the impact of the type of delivery on newborns Thoracoabdominal Mobility METHODS: This is a cross-sectional study with newborns of gestational age between 37 and 41 weeks, of both sexes, with up to 72 hours of life, breathing in ambient air and born by normal delivery or cesarean section. The Thoracoabdominal Mobility was evaluated by videogrammetry using MATLAB Software and considered, in metric units (cm2), as the difference between the highest and lowest thoracoabdominal expansibility for each respiratory cycle RESULTS: Twenty-six infants were included, 11 were male and 50% were born by cesarean section. The mean gestational age was 39 ± 0,9 and 28 ± 18 hours of life. The mobility, difference between greater and lesser expansion, of the thoracic area in vaginal and cesarean delivery was 6 ± 3 cm2 and 7 ± 5 cm2 and the abdominal area was 29 ± 22 cm2 and 21 ± 14 cm2, respectively. This difference was not statistically significant between the two types of delivery for the thoracic area, but was statistically different for the abdominal area (p= 0.01). And the higher the respiratory rate, the lower the abdominal mobility (r= -0.57, p= 0.02 CONCLUSION: The data indicate that the type of delivery seems to influence abdominal mobility and respiratory rate. In the studied sample, newborns with cesarean section presented lower abdominal mobility

INTRODUÇÃO: Nos recém-nascidos de parto cesáreo, ocorre menor compressão torácica e pouca quantidade de líquido é drenada por ação da gravidade, o que diminui, transitoriamente, a mobilidade toracoabdominal OBJETIVO: O objetivo do estudo é avaliar o impacto do tipo de parto na mobilidade torácica e abdominal em recém-nascidos MÉTODO: Trata-se de um estudo transversal com recém-nascidos de idade gestacional entre 37 a 41 semanas, de ambos os sexos, com até 72 horas de vida, respirando em ar ambiente e nascidos de parto normal ou parto cesáreo. A mobilidade torácica e abdominal foram avaliadas pela videogrametria por meio do Software MATLAB e considerada, em unidades métricas (cm2), como a diferença da maior e menor expansibilidade toracoabdominal para cada ciclo respiratório RESULTADOS: Foram inclusos 26 recém-nascidos 11 do sexo masculino e 50% nascidos de parto cesáreo. A idade gestacional média foi de 39 ± 0,9 sem e tinham 28 ± 18 horas de vida. A mobilidade, diferença entre a maior e menor expansibilidade, da área torácica no parto vaginal e cesáreo foi 6 ± 3 cm2 e 7 ± 5 cm2 e da área abdominal foi de 29±22 cm2 e 21± 14 cm2, respectivamente. Esta diferença não foi estatisticamente significante entre os dois tipos de parto para a área torácica, mas mostrou-se diferente estatisticamente para a área abdominal (p = 0,01). E para os recém-nascidos de parto cesáreo, quanto maior a frequência respiratória, menor a mobilidade abdominal (r= -0,57; p = 0,02 CONCLUSÃO: Os dados indicam que o tipo de parto parece influenciar a mobilidade abdominal e a frequência respiratória. Na amostra estudada os recém-nascidos de parto cesáreo apresentaram menor mobilidade abdominal

Tungsten accumulation by highly tolerant marine hydrothermal Sulfitobacter dubius strains carrying a tupBCA cluster.

Tungsten (W) has industrial and economic importance, and is in the European Union list of metals with a high supply risk. It is used by living organisms, which transport it into the cell, in the form of tungstate ion (WO42-), using three different ABC-type transporters from the specific W-uptake system coded by tupABC gene cluster. In this study, strains from a collection recovered from deep-sea hydrothermal sediments were selected according to their ability to tolerate metals and to possess the tup genetic determinants. Three multimetal-tolerant strains, Sulfitobacter dubius NA4, As(V)4 and Sb5, were chosen. The strains were able to grow in the presence of high tungsten concentrations and their growth was unaffected by 1mM tungsten. Moreover, strain Sb5 was able to accumulate up to 52µg W mg-1 protein. Their tup genes were shown to be organized as tupBCA, which is not the most usual gene arrangement. All three strains had the classical TupA conserved motif TTTS, comprising a first Thr replaced by a Val, which seems to be a common feature of the genus Sulfitobacter. This study was an important first step in the exploration of new biological strategies for recovering tungsten from natural or anthropogenic W-impacted environments.

Hospital microbial surface colonization revealed during monitoring of Klebsiella spp., Pseudomonas aeruginosa, and non-tuberculous mycobacteria.

Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital. A total of 173 samples were obtained from the wards Hematology, Urology, Medicine, and Renal Transplants, and 102 presumptive strains recovered. Per sampling, Pseudomonas Isolation agar showed 42.8 to 73.3% of presumptive P. aeruginosa colonies and MacConkey agar recovered mostly Staphylococcus. Most of the colonies recovered in Middlebrook 7H10-PANTA belonged to the genus Methylobacterium. Taps and WC shower curtains carry high bacterial species diversity. The Redundancy Analysis grouped the samples in those mostly handled by patients, and those mostly handled by healthcare staff or of mixed use. This study shows that the preferential users of the space and equipment seem to be important contributors to the microbial community. The most recovered genus was Methylobacterium, known as colonizer of the water distribution system therefore, it is possible that the water points and biofilms in taps also contribute as dispersion hotspots.

Natural hot spots for gain of multiple resistances: arsenic and antibiotic resistances in heterotrophic, aerobic bacteria from marine hydrothermal vent fields.

Microorganisms are responsible for multiple antibiotic resistances that have been associated with resistance/tolerance to heavy metals, with consequences to public health. Many genes conferring these resistances are located on mobile genetic elements, easily exchanged among phylogenetically distant bacteria. The objective of the present work was to isolate arsenic-, antimonite-, and antibiotic-resistant strains and to determine the existence of plasmids harboring antibiotic/arsenic/antimonite resistance traits in phenotypically resistant strains, in a nonanthropogenically impacted environment. The hydrothermal Lucky Strike field in the Azores archipelago (North Atlantic, between 11°N and 38°N), at the Mid-Atlantic Ridge, protected under the OSPAR Convention, was sampled as a metal-rich pristine environment. A total of 35 strains from 8 different species were isolated in the presence of arsenate, arsenite, and antimonite. ACR3 and arsB genes were amplified from the sediment's total DNA, and 4 isolates also carried ACR3 genes. Phenotypic multiple resistances were found in all strains, and 7 strains had recoverable plasmids. Purified plasmids were sequenced by Illumina and assembled by EDENA V3, and contig annotation was performed using the "Rapid Annotation using the Subsystems Technology" server. Determinants of resistance to copper, zinc, cadmium, cobalt, and chromium as well as to the antibiotics ß-lactams and fluoroquinolones were found in the 3 sequenced plasmids. Genes coding for heavy metal resistance and antibiotic resistance in the same mobile element were found, suggesting the possibility of horizontal gene transfer and distribution of theses resistances in the bacterial population.

Persistence of microbial communities including Pseudomonas aeruginosa in a hospital environment: a potential health hazard.

BACKGROUND: The persistence of microbial communities and how they change in indoor environments is of immense interest to public health. Moreover, hospital acquired infections are significant contributors to morbidity and mortality. Evidence suggests that, in hospital environments agent transfer between surfaces causes healthcare associated infections in humans, and that surfaces are an important transmission route and may act as a reservoir for some of the pathogens.This study aimed to evaluate the diversity of microorganisms that persist on noncritical equipment and surfaces in a main hospital in Portugal, and are able to grow in selective media for Pseudomonas, and relate them with the presence of Pseudomonas aeruginosa. RESULTS: During 2 years, a total of 290 environmental samples were analyzed, in 3 different wards. The percentage of equipment in each ward that showed low contamination level varied between 22% and 38%, and more than 50% of the equipment sampled was highly contaminated. P. aeruginosa was repeatedly isolated from sinks (10 times), from the taps' biofilm (16 times), and from the showers and bedside tables (two times). Two ERIC clones were isolated more than once. The contamination level of the different taps analyzed showed correlation with the contamination level of the hand gels support, soaps and sinks. Ten different bacteria genera were frequently isolated in the selective media for Pseudomonas. Organisms usually associated with nosocomial infections as Stenotrophomonas maltophilia, Enterococcus feacalis, Serratia nematodiphila were also repeatedly isolated on the same equipment. CONCLUSIONS: The environment may act as a reservoir for at least some of the pathogens implicated in nosocomial infections. The bacterial contamination level was related to the presence of humidity on the surfaces, and tap water (biofilm) was a point of dispersion of bacterial species, including potentially pathogenic organisms. The materials of the equipment sampled could not be related to the microbial contamination level. The presence of a disinfectant in the isolation medium suggests that the number of microorganism in the environment could be higher and shows the diversity of disinfectant resistant species. The statistical analysis suggests that the presence of bacteria could increase the risk of transmission by hand manipulation.

O cuidado de enfermagem na visita domiciliar gerontológica: uma perspectiva humanística / Nursing care in home gerontoloy visit: a humanistic perspective / El cuidado de enfermería en la visita domiciliar gerontológica: una perspectiva humanística

Trata-se de um estudo descritivo, qualitativo, do tipo relato de experiência, com a finalidade de integrar conhecimentos teóricos e práticos na solidificação de uma aprendizagem científica adquirida nos componentes curriculares Atenção integral à saúde II e Métodos e modelos assistências de enfermagem, do qual se originou o suporte teórico acerca da teoria de Paterson e Zderad, relacionando-a às vivências e ações assistenciais da Enfermagem Gerontológica na atenção primária à saúde, vivenciada por discentes do Curso de Graduação em Enfermagem da Universidade Federal do Rio Grande do Norte (UFRN). O estudo é descrito por etapas do processo de enfermagem, respaldado pelas teoristas em questão e relacionando ao cuidado ao idoso no domicílio, sob a perspectiva da Visita Domiciliar na Estratégia Saúde da Família. Portanto, a integralidade do cuidado, referenciado pela teoria de enfermagem humanística, nos levou à reflexão sobre o rompimento de uma perspectiva de assistência curativa, para analisar o indivíduo pelo seu potencial humano como membro ativo da comunidade, construir seu bem-estar e vir-à-ser demonstrando sua existencialidade.

This is a descriptive and qualitative study, experience report type, with the aim of integrating theoretical and practical knowledge in the solidification of scientific learning gained in curricular components: Integral attention to health II and method and nursing models assistance, which originated the theoretical support for the theory of Paterson and Zderad, relating it to the experiences and aassistance ctions of Gerontological Nursing in primary health care, experienced by students of the Undergraduate degree in nursing from the Federal University of Rio Grande do Norte (UFRN). The study is described by steps of the nursing process supported by these theorists, and relating to the care to the elderly at home, from the perspective of the Domiciliary Visit in the Family Health Strategy. Therefore, the completeness of care referenced by the humanistic nursing theory, took us to reflection about the disruption of a curative assistance perspective, to analyze the individual by his human potential as an active member of the community, building his well-being and demonstrating his existencial.

Este es un estudio descriptivo con abordaje cualitativo del tipo de relato de experiencia, con el objetivo de integrar los conocimientos teóricos y prácticos en la solidificación de la formación científica adquirida en las disciplinas: Integral de Salud II y el método de enfermería y los modelos de asistencias, que se resumieron en el soporte teórico de la teoría de Paterson y Zderad, en relación con las experiencias y acciones de cuidado gerontológico en la atención primaria de salud, experimentadas por los estudiantes del pregrado de Enfermería, Universidad Federal de Rio Grande do Norte (UFRN). El estudio se describe los pasos del proceso de enfermería con el apoyo de los teóricos de que se trate, por lo que la presentación de informes sobre personas mayores visitas de atención domiciliaria. Portar, la atención integral al que hace referencia la teoría de la enfermería humanística a reflexionar sobre la ruptura del enfoque curativo, y por lo tanto, al analizar el potencial humano individual para construir su bienestar por venir y ser un miembro activo de la comunidad y demostrar su existencial.

A seguridade social no Brasil e os obstáculos institucionais à sua implementaçäo / The social security in Brazil and the institutional obstacles to your implementation

Estuda a questäo da seguridade social no Brasil. Aborda alguns dos principais aspectos que têm condicionado o funcionamento de nosso sistema de proteçäo social, com ênfase na sua dimensäo político-administrativa. Analisa a influência dos problemas de natureza político-administrativa sobre o processo de concretizaçäo da seguridade social no Brasil. Busca estabelecer um referencial conceitual e histórico, a partir da descriçäo do processo de evoluçäo das políticas sociais de Estado, com destaque para as trajetórias dos países europeus e do Brasil. Procura caracterizar os modelos de proteçäo social que se difundiram pelo mundo e foram aplicados em nosso país até meados de 80, influenciando os constituintes nacionais ainda no final daquela década. Busca identificar elementos comuns e as abordagens teóricas, pelas quais foram influenciados os parlamentares brasileiros ao final da década de 80, quando da definiçäo do sistema de seguridade adotado pela atual Constituiçäo federal. Descreve e analisa os problemas constatados na operacionalizaçäo recente das políticas públicas referentes aos três componentes básicos de nosso sistema de seguridade social, quais sejam: saúde, assistência e previdência social. Analisa a gestäo do sistema de seguridade social tendo como referência um conjunto de quatro importantes variáveis do atual ciclo de gestäo no setor público: os processos de planejamento e orçamentaçäo, as estruturas organizacionais, as práticas diretivas e de controle e a articulaçäo intergovernamental. Enuncia algumas propostas com vista à consolidaçäo de nosso sistema de seguridade social, tendo como princípios a descentralizaçäo, o controle social e a manutençäo de um permanente processo de avaliaçäo institucional.