Assuntos
Antropometria , Desenvolvimento Infantil , Idade Gestacional , Humanos , Lactente , Recém-NascidoRESUMO
OBJECTIVES: To analyze the presence of bovine beta-LG in breast milk. METHODS: Human milk samples from 14 healthy non-atopic women on diets with different cow's milk contents were examined. The total concentration of beta-LG immuno-like proteins (beta-LGIP) was determined by enzyme linked immunosorbent assay (ELISA). Identification of antigens was done by N-terminal sequencing. RESULTS: beta-LGIP reactivity of the milk from subjects on different diets was not significantly different. Human lactoferrin, beta-casein and alpha-lactalbumin, were identified as cross-reacting antigens. CONCLUSIONS: False-positive results in ELISA determinations of bovine beta-LG in human milk might be due to cross-reactions between polyclonal antibodies and different protein antigens.
Assuntos
Lactoglobulinas/análise , Leite Humano/química , Leite/química , Animais , Bovinos , Cromatografia de Afinidade , Dieta , Ensaio de Imunoadsorção Enzimática/métodos , Ensaio de Imunoadsorção Enzimática/estatística & dados numéricos , Reações Falso-Positivas , Feminino , Humanos , Reprodutibilidade dos Testes , Fatores de TempoRESUMO
Triploidy is a complete extra set of chromosomes and results in a clinically recognizable lethal syndrome with hydatiform placental changes, severe intrauterine growth deficiency, cranial bone abnormalities, eye defects, malformed ears, syndactyly, genital anomalies, congenital heart defects and brain anomalies. Less severe are instances of diploid-tetraploid mixoploidy and patients are more likely to survive. The present report is another contribution to the knowledge of human triploidy: we describe a 69,XXY triploid male occurred to our observation.
