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1.
Mol Microbiol ; 112(3): 1024-1040, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31286583

RESUMO

Differentiation of Trypanosoma brucei, a flagellated protozoan parasite, between life cycle stages typically occurs through an asymmetric cell division process, producing two morphologically distinct daughter cells. Conversely, proliferative cell divisions produce two daughter cells, which look similar but are not identical. To examine in detail differences between the daughter cells of a proliferative division of procyclic T. brucei we used the recently identified constituents of the flagella connector. These segregate asymmetrically during cytokinesis allowing the new-flagellum and the old-flagellum daughters to be distinguished. We discovered that there are distinct morphological differences between the two daughters, with the new-flagellum daughter in particular re-modelling rapidly and extensively in early G1. This re-modelling process involves an increase in cell body, flagellum and flagellum attachment zone length and is accompanied by architectural changes to the anterior cell end. The old-flagellum daughter undergoes a different G1 re-modelling, however, despite this there was no difference in G1 duration of their respective cell cycles. This work demonstrates that the two daughters of a proliferative division of T. brucei are non-equivalent and enables more refined morphological analysis of mutant phenotypes. We suggest all proliferative divisions in T. brucei and related organisms will involve non-equivalence.


Assuntos
Flagelos/metabolismo , Trypanosoma brucei brucei/citologia , Divisão Celular , Proliferação de Células , Citocinese , Flagelos/genética , Estágios do Ciclo de Vida , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/crescimento & desenvolvimento , Trypanosoma brucei brucei/metabolismo
2.
PLoS One ; 8(5): e61319, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23658690

RESUMO

BACKGROUND: Staphylococcus aureus is a major cause of healthcare associated mortality, but like many important bacterial pathogens, it is a common constituent of the normal human body flora. Around a third of healthy adults are carriers. Recent evidence suggests that evolution of S. aureus during nasal carriage may be associated with progression to invasive disease. However, a more detailed understanding of within-host evolution under natural conditions is required to appreciate the evolutionary and mechanistic reasons why commensal bacteria such as S. aureus cause disease. Therefore we examined in detail the evolutionary dynamics of normal, asymptomatic carriage. Sequencing a total of 131 genomes across 13 singly colonized hosts using the Illumina platform, we investigated diversity, selection, population dynamics and transmission during the short-term evolution of S. aureus. PRINCIPAL FINDINGS: We characterized the processes by which the raw material for evolution is generated: micro-mutation (point mutation and small insertions/deletions), macro-mutation (large insertions/deletions) and the loss or acquisition of mobile elements (plasmids and bacteriophages). Through an analysis of synonymous, non-synonymous and intergenic mutations we discovered a fitness landscape dominated by purifying selection, with rare examples of adaptive change in genes encoding surface-anchored proteins and an enterotoxin. We found evidence for dramatic, hundred-fold fluctuations in the size of the within-host population over time, which we related to the cycle of colonization and clearance. Using a newly-developed population genetics approach to detect recent transmission among hosts, we revealed evidence for recent transmission between some of our subjects, including a husband and wife both carrying populations of methicillin-resistant S. aureus (MRSA). SIGNIFICANCE: This investigation begins to paint a picture of the within-host evolution of an important bacterial pathogen during its prevailing natural state, asymptomatic carriage. These results also have wider significance as a benchmark for future systematic studies of evolution during invasive S. aureus disease.


Assuntos
Evolução Molecular , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/genética , Adulto , Infecções Assintomáticas , Portador Sadio , Genoma Bacteriano , Humanos , Mutação INDEL , Nariz/microbiologia , Polimorfismo de Nucleotídeo Único , Seleção Genética , Análise de Sequência de DNA , Infecções Estafilocócicas/transmissão
3.
Cytoskeleton (Hoboken) ; 69(11): 931-41, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23027706

RESUMO

Cytokinesis is a crucial step in the cell division cycle whereby the cell membrane and underlying cortex is remodelled and drawn together to create two new daughter cells. While in many eukaryotic systems this process is accomplished by an actomyosin contractile ring, the protozoan parasite Trypanosoma brucei displays an unusual mechanism for cytokinesis, with an increased reliance on microtubules. There are a number of crucial preparatory steps involving the replication and segregation of organelles that must be undertaken in order for cytokinesis to occur. In this review, we will discuss the cellular architecture of the trypanosome and its importance within cytokinesis, and the recent progress in understanding the regulatory systems involved. Recent advances in three-dimensional imaging techniques have improved our understanding of the mechanisms driving cytokinesis and are likely to yield further insights in the future.


Assuntos
Citocinese/fisiologia , Microtúbulos/metabolismo , Trypanosoma brucei brucei/fisiologia , Microtúbulos/genética
4.
Cell Rep ; 2(1): 185-97, 2012 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-22840408

RESUMO

Identification of replication initiation sites, termed origins, is a crucial step in understanding genome transmission in any organism. Transcription of the Trypanosoma brucei genome is highly unusual, with each chromosome comprising a few discrete transcription units. To understand how DNA replication occurs in the context of such organization, we have performed genome-wide mapping of the binding sites of the replication initiator ORC1/CDC6 and have identified replication origins, revealing that both localize to the boundaries of the transcription units. A remarkably small number of active origins is seen, whose spacing is greater than in any other eukaryote. We show that replication and transcription in T. brucei have a profound functional overlap, as reducing ORC1/CDC6 levels leads to genome-wide increases in mRNA levels arising from the boundaries of the transcription units. In addition, ORC1/CDC6 loss causes derepression of silent Variant Surface Glycoprotein genes, which are critical for host immune evasion.


Assuntos
Replicação do DNA/genética , Genoma de Protozoário , Origem de Replicação/fisiologia , Análise de Sequência de DNA , Transcrição Gênica/genética , Trypanosoma brucei brucei/genética , Sítios de Ligação/genética , Epistasia Genética , Regulação da Expressão Gênica , Genoma de Protozoário/genética , Modelos Biológicos , Complexo de Reconhecimento de Origem/análise , Complexo de Reconhecimento de Origem/metabolismo , Origem de Replicação/genética
5.
PLoS One ; 7(3): e32674, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22412905

RESUMO

DNA replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with Cdt1. Eukaryotic ORC is considered to be composed of six subunits, named Orc1-6, and monomeric Cdc6 is closely related in sequence to Orc1. However, ORC has been little explored in protists, and only a single ORC protein, related to both Orc1 and Cdc6, has been shown to act in DNA replication in Trypanosoma brucei. Here we identify three highly diverged putative T. brucei ORC components that interact with ORC1/CDC6 and contribute to cell division. Two of these factors are so diverged that we cannot determine if they are eukaryotic ORC subunit orthologues, or are parasite-specific replication factors. The other we show to be a highly diverged Orc4 orthologue, demonstrating that this is one of the most widely conserved ORC subunits in protists and revealing it to be a key element of eukaryotic ORC architecture. Additionally, we have examined interactions amongst the T. brucei MCM subunits and show that this has the conventional eukaryotic heterohexameric structure, suggesting that divergence in the T. brucei replication machinery is limited to the earliest steps in origin licensing.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Complexo de Reconhecimento de Origem/metabolismo , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/metabolismo , Sequência de Aminoácidos , Proteínas de Ciclo Celular/química , DNA Helicases/química , DNA Helicases/classificação , DNA Helicases/genética , Dados de Sequência Molecular , Complexo de Reconhecimento de Origem/química , Filogenia , Ligação Proteica , Multimerização Proteica , Subunidades Proteicas/genética , Interferência de RNA , Alinhamento de Sequência
6.
Cell Motil Cytoskeleton ; 66(1): 24-35, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19009637

RESUMO

Eukaryotic cilia and flagella are highly conserved structures composed of a canonical 9+2 microtubule axoneme. Several recent proteomic studies of cilia and flagella have been published, including a proteome of the flagellum of the protozoan parasite Trypanosoma brucei. Comparing proteomes reveals many novel proteins that appear to be widely conserved in evolution. Amongst these, we found a previously uncharacterised protein which localised to the axoneme in T. brucei, and therefore named it Trypanosome Axonemal protein (TAX)-2. Ablation of the protein using RNA interference in the procyclic form of the parasite has no effect on growth but causes a reduction in motility. Using transmission electron microscopy, various structural defects were seen in some axonemes, most frequently with microtubule doublets missing from the 9+2 arrangement. RNAi knockdown of TAX-2 expression in the bloodstream form of the parasite caused defects in growth and cytokinesis, a further example of the effects caused by loss of flagellar function in bloodstream form T. brucei. In procyclic cells we used a new set of vectors to ablate protein expression in cells expressing a GFP:TAX-2 fusion protein, which enabled us to easily quantify protein reduction and visualise axonemes made before and after RNAi induction. This establishes a useful generic technique but also revealed a specific observation that the new flagellum on the daughter trypanosome continues growth after cytokinesis. Our results provide evidence for TAX-2 function within the axoneme, where we suggest that it is involved in processes linking the outer doublet microtubules and the central pair.


Assuntos
Axonema/metabolismo , Divisão Celular , Sequência Conservada , Evolução Molecular , Proteínas de Protozoários/química , Proteínas de Protozoários/metabolismo , Trypanosoma brucei brucei/citologia , Sequência de Aminoácidos , Animais , Axonema/ultraestrutura , Movimento Celular , Biologia Computacional , Citocromos b5/química , Citocinese , Flagelos/ultraestrutura , Proteínas de Fluorescência Verde/metabolismo , Dados de Sequência Molecular , Estrutura Terciária de Proteína , Transporte Proteico , Interferência de RNA , Trypanosoma brucei brucei/crescimento & desenvolvimento , Trypanosoma brucei brucei/ultraestrutura
7.
BMC Biol ; 5: 33, 2007 Aug 07.
Artigo em Inglês | MEDLINE | ID: mdl-17683645

RESUMO

BACKGROUND: Impairment of cilia and flagella function underlies a growing number of human genetic diseases. Mutations in hydin in hy3 mice cause lethal communicating hydrocephalus with early onset. Hydin was recently identified as an axonemal protein; however, its function is as yet unknown. RESULTS: Here we use RNAi in Trypanosoma brucei to address this issue and demonstrate that loss of Hydin causes slow growth and a loss of cell motility. We show that two separate defects in newly-formed flagellar central pair microtubules underlie the loss of cell motility. At early time-points after RNAi induction, the central pair becomes mispositioned, while at later time points the central pair is lost. While the basal body is unaffected, both defects originate at the basal plate, reflecting a role for TbHydin throughout the length of the central pair. CONCLUSION: Our data provide the first evidence of Hydin's role within the trypanosome axoneme, and reveal central pair anomalies and thus impairment of ependymal ciliary motility as the likely cause of the hydrocephalus observed in the hy3 mouse.


Assuntos
Hidrocefalia/genética , Proteínas dos Microfilamentos/genética , Microtúbulos , Proteínas de Protozoários/fisiologia , Trypanosoma brucei brucei/genética , Animais , Sequência de Bases , Primers do DNA , Camundongos , Interferência de RNA
8.
J Cell Sci ; 120(Pt 1): 7-15, 2007 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-17182899

RESUMO

Cilia, either motile or immotile, exist on most cells in the human body. There are several different mechanisms of ciliogenesis, which enable the production of many kinds of cilia and flagella: motile and immotile, transient and long-lived. These can be linked to the cell cycle or associated with differentiation. A primary cilium is extended from a basal body analogous to the mitotic centrioles, whereas the several hundred centrioles needed to form the cilia of a multi-ciliated cell can be generated by centriolar or acentriolar pathways. Little is known about the molecular control of these pathways and most of our knowledge comes from ultrastructural studies. The increasing number of genetic diseases linked to dysfunctional cilia and basal bodies has renewed interest in this area, and recent proteomic and cell biological studies in model organisms have helped to shed light on the molecular components of these enigmatic organelles.


Assuntos
Movimento Celular/fisiologia , Centríolos/fisiologia , Cílios/fisiologia , Células Epiteliais/fisiologia , Flagelos/fisiologia , Animais , Centríolos/ultraestrutura , Células Epiteliais/ultraestrutura
9.
Nature ; 440(7081): 224-7, 2006 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-16525475

RESUMO

The 9 + 2 microtubule axoneme of flagella and cilia represents one of the most iconic structures built by eukaryotic cells and organisms. Both unity and diversity are present among cilia and flagella on the evolutionary as well as the developmental scale. Some cilia are motile, whereas others function as sensory organelles and can variously possess 9 + 2 and 9 + 0 axonemes and other associated structures. How such unity and diversity are reflected in molecular repertoires is unclear. The flagellated protozoan parasite Trypanosoma brucei is endemic in sub-Saharan Africa, causing devastating disease in humans and other animals. There is little hope of a vaccine for African sleeping sickness and a desperate need for modern drug therapies. Here we present a detailed proteomic analysis of the trypanosome flagellum. RNA interference (RNAi)-based interrogation of this proteome provides functional insights into human ciliary diseases and establishes that flagellar function is essential to the bloodstream-form trypanosome. We show that RNAi-mediated ablation of various proteins identified in the trypanosome flagellar proteome leads to a rapid and marked failure of cytokinesis in bloodstream-form (but not procyclic insect-form) trypanosomes, suggesting that impairment of flagellar function may provide a method of disease control. A postgenomic meta-analysis, comparing the evolutionarily ancient trypanosome with other eukaryotes including humans, identifies numerous trypanosome-specific flagellar proteins, suggesting new avenues for selective intervention.


Assuntos
Sangue/parasitologia , Flagelos/fisiologia , Movimento , Trypanosoma brucei brucei/citologia , Trypanosoma brucei brucei/fisiologia , Animais , Biologia Computacional , Flagelos/química , Flagelos/genética , Humanos , Fenótipo , Proteoma/genética , Proteoma/metabolismo , Proteômica , Proteínas de Protozoários/genética , Proteínas de Protozoários/metabolismo , Interferência de RNA , Trypanosoma brucei brucei/genética
10.
J Cell Sci ; 118(Pt 23): 5421-30, 2005 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-16278296

RESUMO

Eukaryotic cilia and flagella are highly conserved structures composed of a canonical 9+2 microtubule axoneme. Comparative genomics of flagellated and non-flagellated eukaryotes provides one way to identify new putative flagellar proteins. We identified the Parkin co-regulated gene, or PACRG, from such a screen. Male mice deficient in PACRG are sterile, but its function has been little explored. The flagellated protozoan parasite Trypanosoma brucei possesses two homologues of PACRG. We performed RNA interference knockdown experiments of the two genes independently and both together. Simultaneous ablation of both proteins produced slow growth and paralysis of the flagellum with consequent effects on organelle segregation. Moreover, using transmission electron microscopy, structural defects were seen in the axoneme, with microtubule doublets missing from the canonical 9+2 formation. The occurrence of missing doublets increased toward the distal end of the flagellum and sequential loss of doublets was observed along individual axonemes. GFP fusion proteins of both PACRG homologues localised along the full length of the axoneme. Our results provide the first evidence for PACRG function within the axoneme, where we suggest that PACRG acts to maintain functional stability of the axonemal outer doublets of both motile and sensory cilia and flagella.


Assuntos
Flagelos/metabolismo , Proteínas Associadas aos Microtúbulos/fisiologia , Microtúbulos/fisiologia , Morfogênese/fisiologia , Proteínas de Protozoários/fisiologia , Trypanosoma brucei brucei/crescimento & desenvolvimento , Sequência de Aminoácidos , Animais , Sequência Conservada , Flagelos/ultraestrutura , Proteínas Associadas aos Microtúbulos/genética , Microtúbulos/genética , Microtúbulos/ultraestrutura , Dados de Sequência Molecular , Proteínas de Protozoários/genética , Interferência de RNA/fisiologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Homologia de Sequência de Aminoácidos , Trypanosoma brucei brucei/genética , Trypanosoma brucei brucei/ultraestrutura
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