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ObjectiveTo observe the effect of estrogenic-like effects ofWujia-Shenghua capsule and effective medication site.MethodsThrough D-101 macroporous resin column methods,Wujia-Shenghua capsule with 60% ethanol extraction was separated for water elution part, 20% ethanol elution part, 40% ethanol elution part, 60% ethanol elution part. Then each elution part was respectively mixed into concentration of 10mg/ml,1mg/ml, 0.1mg/ml, 0.01 mg/ml and acted on the MCF-7 cell to have, MTT test and rate of PR calculation.Results Compared with the blank control group(100%), when the concentration was 0.1 mg/ml, water elution part, 20% ethanol elution part and 60% ethanol elution part(98.10%, 101.06%, 106.04%)had no effect on the proliferation of MCF-7 cells, there was not statistically significant(P>0.05), while the 40% ethanol elution part(108.22%)can promote the proliferation of MCF-7 cells,there was a statistically significant(P<0.05). When the concentration was 1 mg/ml, 20%, 40% and 60% ethanol elution part(111.72%, 122.48%, 115.35%)can distinctly promote the proliferation of MCF-7 cells, there was a significant difference between four groups(P<0.01).ConclusionThe 40% ethanol elution part ofWujia-shenghua capsule has the strongest estrogen activity on plant.
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Objective To investigate the liver protective effects of Xiaoyaosan in carbon tetrachloride-induced acute liver injury in mice.Methods Thirty mice were randomly divided into a control group, a model group, and aXiaoyaosan group, with 10 mice in each group. The mice in theXiaoyaosan group were intragastrically administrated withXiaoyaosan, the mice in the remaining two groups were fed with an equal volume of distilled water. After 7 days, acute liver injury were inducedvia intraperitoneal injection of carbon tetrachloride peanut oil solution. The serum levels of alanine transaminase(ALT), aspartate transaminase (AST), malondialdehyde(MDA)and superoxide dismutase(SOD)were measured, and pathological changes of liver tissue was tested after 16 hours.Results The serum levels of ALT(136.46±15.75 U/Lvs. 22.96±6.23 U/L), AST(145.37±16.39 U/Lvs. 31.89±7.26 U/L), and MDA level in the liver tissue(17.48±3.45 nmol/mgvs. 4.22±1.08 nmol/mg)in the model group were significantly higher than those in the control group(allP<0.01), SOD level in the liver tissue significantly lower than that in the control group(261.60±20.29 U/mgvs. 336.73±25.34 U/mg,P<0.01). The serum levels of ALT(89.38±6.96 U/L,P<0.01), AST(119.04±20.44 U/L, P<0.05), MDA level(10.30±2.22 nmol/mg,P<0.01) in the liver tissue in theXiaoyaosan group levels were significantly lower than those in the model group, and SOD level(304.77±31.71 U/mg,P<0.01) in the liver tissue were significantly higher than that in the model group.ConclusionXiaoyaosan has liver protective effects in carbon tetrachloride-induced acute liver injury in mice.
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This study was aimed to establish GC-MS fingerprint of Bu-yang Huan-wu (BYHW) decoction. The 5HP-MS quartz capillary column was used. The temperature program carrier gas was He. The velocity was 1.0 mL·min-1. The injection volume was 1 μL. The split ratio was 1:20. The MS conditions were electron impact (EI) ion source with the transmission line temperature at 280oC, ion source temperature at 230oC, and quadrupole temperature at 150oC. The mass scan range was m/z 50~550. And the results were contrasted with ChemStation and NIST 05a. The results showed that nine common peaks were obtained in the fingerprint of eleven batches of BYHW decoction by peaks matched with the Similarity Evaluation System for Chromatographic Fingerprint (2004 A Version). The similari-ty of fingerprints was calculated more than 0.93. It was concluded that fingerprinting can be used as a method of quality control and provide evidence for the research of volatile ingredient of BYHW decoction.
