1.
Methods Mol Biol
; 2049: 73-85, 2019.
Artigo
em Inglês
| MEDLINE
| ID: mdl-31602605
RESUMO
Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.