Comparison among three different serological methods for the detection of equine influenza virus infection.

The equine influenza virus (EIV) H3N8 subtype is responsible for all EIV outbreaks worldwide while the H7N7 subtype is less pathogenic and is considered extinct as it has not been confirmed in outbreaks since 1980. Although EIV is enzootic in Brazil, few reports describe the actual EIV antibody status in the country. The aims of this study were: - to evaluate the efficiency of different serum treatments described by the World Organisation for Animal Health (OIE) and the World Health Organization (WHO) to remove non-specific haemagglutination inhibitors for the haemagglutination inhibition (HI) assay for EIV - to evaluate the presence of EIV antibodies by HI, enzyme-linked immunosorbent assay and agar gel immunodiffusion in 83 non-vaccinated equines from São Paulo State - to evaluate a strategy to better analyse equine sera for EIV antibodies. Although there was no statistical difference among treatments, receptor-destroying enzyme treatment followed by chicken erythrocyte adsorption showed more consistent results, which corroborate the OIE and WHO recommendation to use this treatment preferentially. The HI results suggest equine H3N8 virus circulation among the animals tested from São Paulo State. The algorithm suggested here could be used to guide antibody detection against equine influenza virus in equines, improving the test specificity by aiming to avoid false positive results.

Tous les foyers de grippe équine dans le monde sont dus au sous-type H3N8 du virus. Le sous-type H7N7, moins pathogène, est considéré comme éteint, sa présence n'ayant été confirmée dans aucun des foyers enregistrés depuis 1980. Au Brésil, la grippe équine est enzootique mais la prévalence d'anticorps dans le pays est peu documentée. La présente étude avait trois objectifs : ­ évaluer l'efficacité de plusieurs traitements de sérums décrits par l'Organisation mondiale de la santé animale (OIE) et l'Organisation mondiale de la santé (OMS) sur la suppression des inhibiteurs d'hémagglutination non spécifiques, afin de pouvoir utiliser l'épreuve d'inhibition de l'hémagglutination pour la détection de la grippe équine, ­ évaluer la présence d'anticorps dirigés contre la grippe équine chez 83 chevaux non vaccinés de l'état de São Paulo en utilisant l'inhibition de l'hémagglutination, l'épreuve immuno-enzymatique (ELISA) et l'épreuve d'immunodiffusion en gélose (IDG) ; ­ évaluer une stratégie visant à améliorer les techniques sérologiques de détection des anticorps dirigés contre la grippe équine. S'il n'y a pas eu de différence statistique significative entre les traitements, celui faisant appel à l'enzyme de destruction du récepteur suivi d'une adsorption sur érythrocytes de poule a permis d'obtenir les résultats les plus cohérents, ce qui corrobore les recommandations de l'OIE et de l'OMS en faveur de ce traitement. Les résultats obtenus au moyen de l'inhibition de l'hémagglutination indiquent que le virus H3N8 est présent parmi les animaux testés de l'état de São Paulo. L'algorithme présenté par les auteurs pourrait servir de modèle pour détecter la présence d'anticorps dirigés contre le virus de la grippe équine chez les chevaux : en effet, il permet d'éviter les résultats faussement positifs, ce qui améliore la spécificité du test utilisé.

El subtipo H3N8 del virus de la gripe equina (VGE) es el agente etiológico de todos los brotes que se producen en el mundo, mientras que el subtipo H7N7, menos patogénico, se da por extinto, en la medida en que desde 1980 no se ha confirmado su intervención en brote alguno. Aunque en el Brasil el VGE es enzoótico, existen pocos trabajos que den cuenta de la situación real del país en cuanto a la presencia de anticuerpos contra el virus. Los autores describen un estudio que perseguía los siguientes objetivos: ­ evaluar la eficacia de distintos tratamientos séricos descritos por la Organización Mundial de Sanidad Animal (OIE) y la Organización Mundial de la Salud (OMS) para eliminar los inhibidores inespecíficos de la hemaglutinación con objeto de aplicar la técnica de inhibición de la hemaglutinación a la detección del VGE; ­ evaluar la presencia de anticuerpos contra el VGE por inhibición de la hemaglutinación, ensayo inmunoenzimático (ELISA) e inmunodifusión en gel de agar en 83 ejemplares equinos no vacunados del estado de São Paulo; ­ evaluar una estrategia encaminada a analizar más eficazmente sueros equinos para detectar en ellos anticuerpos anti-VGE. Aunque no se observaron diferencias estadísticamente significativas entre los tratamientos, el uso de enzimas destructores de receptores seguido de la técnica de adsorción de eritrocitos de pollo arrojó resultados más coherentes, cosa que avala la recomendación de la OIE y la OMS de privilegiar este tratamiento. Los resultados obtenidos por inhibición de la hemaglutinación parecen indicar que el virus H3N8 equino circula entre los animales analizados del estado de São Paulo. El algoritmo aquí propuesto podría servir de guía para detectar en equinos la presencia de anticuerpos contra el VGE. Puesto que apunta a evitar falsos positivos, su aplicación mejoraría la especificidad de la prueba.

The influence of vibrissal somatosensory processing in rat superior colliculus on prey capture.

The lateral part of intermediate layer of superior colliculus (SCl) is a critical substrate for successful predation by rats. Hunting-evoked expression of the activity marker Fos is concentrated in SCl while prey capture in rats with NMDA lesions in SCl is impaired. Particularly affected are rapid orienting and stereotyped sequences of actions associated with predation of fast moving prey. Such deficits are consistent with the view that the deep layers of SC are important for sensory guidance of movement. Although much of the relevant evidence involves visual control of movement, less is known about movement guidance by somatosensory input from vibrissae. Indeed, our impression is that prey contact with whiskers is a likely stimulus to trigger predation. Moreover, SCl receives whisker and orofacial somatosensory information directly from trigeminal complex, and indirectly from zona incerta, parvicelular reticular formation and somatosensory barrel cortex. To better understand sensory guidance of predation by vibrissal information we investigated prey capture by rats after whisker removal and the role of superior colliculus (SC) by comparing Fos expression after hunting with and without whiskers. Rats were allowed to hunt cockroaches, after which their whiskers were removed. Two days later they were allowed to hunt cockroaches again. Without whiskers the rats were less able to retain the cockroaches after capture and less able to pursue them in the event of the cockroach escaping. The predatory behaviour of rats with re-grown whiskers returned to normal. In parallel, Fos expression in SCl induced by predation was significantly reduced in whiskerless animals. We conclude that whiskers contribute to the efficiency of rat prey capture and that the loss of vibrissal input to SCl, as reflected by reduced Fos expression, could play a critical role in predatory deficits of whiskerless rats.

3-D retinal surface inference: stereo or monocular fundus camera?

Three-dimensional (3-D) imaging of the eye fundus, and in particular of the optic disc, is widely used to assess glaucoma progression over time. In the literature, 3-D images of the optic disc have been obtained from stereo and monocular fundus cameras. While stereo systems are the gold standard for optic disc examination, monocular systems are less expensive, and therefore of more practical use. This stimulated a thorough investigation of the limits and advantages of these two imaging modalities. Our conclusion is that monocular imaging is generally not suitable for 3-D estimation. This is attributed to the fact that monocular systems do not allow a change in the vantage point from which the retinal surface is observed, despite variations in the relative pose between the eye and the fundus camera. To validate this analysis we carry out several experiments on both stereo and monocular fundus cameras with standard 3-D reconstruction algorithms. Furthermore, we devise a calibration procedure to quantify experimentally the highest accuracy achievable with a stereo system.

A geometric approach to shape from defocus.

We introduce a novel approach to shape from defocus, i.e., the problem of inferring the three-dimensional (3D) geometry of a scene from a collection of defocused images. Typically, in shape from defocus, the task of extracting geometry also requires deblurring the given images. A common approach to bypass this task relies on approximating the scene locally by a plane parallel to the image (the so-called equifocal assumption). We show that this approximation is indeed not necessary, as one can estimate 3D geometry while avoiding deblurring without strong assumptions on the scene. Solving the problem of shape from defocus requires modeling how light interacts with the optics before reaching the imaging surface. This interaction is described by the so-called point spread function (PSF). When the form of the PSF is known, we propose an optimal method to infer 3D geometry from defocused images that involves computing orthogonal operators which are regularized via functional singular value decomposition. When the form of the PSF is unknown, we propose a simple and efficient method that first learns a set of projection operators from blurred images and then uses these operators to estimate the 3D geometry of the scene from novel blurred images. Our experiments on both real and synthetic images show that the performance of the algorithm is relatively insensitive to the form of the PSF. Our general approach is to minimize the Euclidean norm of the difference between the estimated images and the observed images. The method is geometric in that we reduce the minimization to performing projections onto linear subspaces, by using inner product structures on both infinite and finite-dimensional Hilbert spaces. Both proposed algorithms involve only simple matrix-vector multiplications which can be implemented in real-time.

[Hormone replacement therapy and the wellbeing of menopausal women]. / Terapia ormonale sostitutiva e benessere della donna in menopausa.

BACKGROUND AND AIMS: The authors performed a retrospective analysis of 682 patients attending the Menopause Centre from 1994 to today. These patients were referred for a wide variety of symptoms occurring in premenopause and menopause. METHODS: All patients initially underwent general screening which was followed by appropriate therapy on the basis of objective examination, blood chemical and instrumental tests, and the subjective clinical situation. RESULTS AND CONCLUSIONS: It was found that the most frequently used treatment was sequential or combined oestroprogestin replacement treatment (63%), with tibolone in 9% of cases and veralipride in 5%. The best results from the point of view of the improved wellbeing of the woman were obtained using HRT and tibolone.

[Relation between Doppler echo-flowmetry and oncogenic markers in pelvic masses]. / Rapporto tra ecoflussometria Doppler e marker oncogeni nelle masse pelviche.

BACKGROUND: The differential diagnosis between benign and malignant pelvic masses is still difficult and is based on clinical semeiotics and echography, whereas a reliable diagnosis can only be obtained by histological analysis. METHODS: A total of 40 patients were examined using transvaginal Doppler echoflowmetry which was used to evaluate the vascular distribution and intralesional and perilesional flowmetric values. These values were then compared with oncogenic markers and with histological tests of the neoformations. RESULTS: Histological analysis revealed that a flow with high PI is a marker of benignancy, whereas a low PI value tends to show malignancy. On the contrary oncogenic markers reveal a high level of sensitivity but low specificity, especially CA 125.

Increasing the relevancy and usefulness of programme evaluation: a comprehensive evaluation.

Most organizations implement evaluation research to determine how to improve a programme or to decide if a programme should be continued. However, evaluation research can be used to help organizations make relevant and valid decisions for the cycle of programme planning, programme development, programme implementation, and programme operation. This paper describes a "comprehensive evaluation model" which will provide a framework for understanding the importance of evaluation activities at all programme phases (i.e. planning, development, implementation, and operation). Four sequential evaluation stages are presented as being the framework for the "comprehensive evaluation model". It is argued that these stages are essential to the organization and the evaluator in helping them adequately provide an assessment of a programme's efficiency and effectiveness.