A comparison of canine whole and intact parathyroid hormone concentrations as measured by different assays.

OBJECTIVES: To compare an intact immunoradiometric parathyroid hormone assay with (1) a non-isotopic technique; and, (2) a whole parathyroid hormone immunoradiometric assay. MATERIALS AND METHODS: Intact parathyroid hormone concentrations were measured using immunoradiometric (Scantibodies) and chemiluminescent (Immulite 2000) assays. Whole parathyroid hormone concentration was measured using an immunoradiometric assay (Scantibodies). RESULTS: A total of 48 and 47 samples, respectively, were used to compare immunoradiometric and chemiluminescent intact parathyroid concentrations and intact and whole parathyroid hormone concentrations by immunoradiometric assays. Using chemiluminescence, 39 (81.3%) samples had intact parathyroid hormone concentrations at or below the reported limit of detection of the assay (0.3 pmol/L). Intact [6.3 (2.0 to 95.5) pmol/L] and whole [3.3 (0.8 to 125.2) pmol/L] immunoradiometric parathyroid hormone concentrations exhibited excellent correlation. CLINICAL SIGNIFICANCE: Not all parathyroid hormone assays perform similarly. The chemiluminescent assay in this study cannot be recommended for use in dogs. The immunoradiometric intact parathyroid hormone assay proved to be a more reliable method. Given the correlation between intact and whole parathyroid hormone concentrations, it remains unclear which one is superior for routine clinical decision-making.

Replication of the association between variants in WFS1 and risk of type 2 diabetes in European populations.

AIMS/HYPOTHESIS: Mutations at the gene encoding wolframin (WFS1) cause Wolfram syndrome, a rare neurological condition. Associations between single nucleotide polymorphisms (SNPs) at WFS1 and type 2 diabetes have recently been reported. Thus, our aim was to replicate those associations in a northern Swedish case-control study of type 2 diabetes. We also performed a meta-analysis of published and previously unpublished data from Sweden, Finland and France, to obtain updated summary effect estimates. METHODS: Four WFS1 SNPs (rs10010131, rs6446482, rs752854 and rs734312 [H611R]) were genotyped in a type 2 diabetes case-control study (n = 1,296/1,412) of Swedish adults. Logistic regression was used to assess the association between each WFS1 SNP and type 2 diabetes, following adjustment for age, sex and BMI. We then performed a meta-analysis of 11 studies of type 2 diabetes, comprising up to 14,139 patients and 16,109 controls, to obtain a summary effect estimate for the WFS1 variants. RESULTS: In the northern Swedish study, the minor allele at rs752854 was associated with reduced type 2 diabetes risk [odds ratio (OR) 0.85, 95% CI 0.75-0.96, p=0.010]. Borderline statistical associations were observed for the remaining SNPs. The meta-analysis of the four independent replication studies for SNP rs10010131 and correlated variants showed evidence for statistical association (OR 0.87, 95% CI 0.82-0.93, p=4.5 x 10(-5)). In an updated meta-analysis of all 11 studies, strong evidence of statistical association was also observed (OR 0.89, 95% CI 0.86-0.92; p=4.9 x 10(-11)). CONCLUSIONS/INTERPRETATION: In this study of WFS1 variants and type 2 diabetes risk, we have replicated the previously reported associations between SNPs at this locus and the risk of type 2 diabetes.

PARL Leu262Val is not associated with fasting insulin levels in UK populations.

AIMS/HYPOTHESIS: PARL, the gene encoding presenilins-associated rhomboid-like protein, maps to chromosome 3q27 within a quantitative trait locus that influences components of the metabolic syndrome. Recently, an amino acid substitution (Leu262Val, rs3732581) in PARL was associated with fasting plasma insulin levels in a US white population (N=1031). This variant was also found to modify the positive association between age and fasting insulin. The aim of this study was to test whether these findings could be replicated in two UK population-based cohorts. METHODS: Participants from the Medical Research Council Ely and Hertfordshire cohort studies were genotyped for this variant using a SNaPshot primer extension assay and Taqman assay respectively. Full phenotypic and genotypic data were available for 3,666 study participants. RESULTS: Based on a dominant model, we found no association between the Leu262Val polymorphism and fasting insulin levels (p=0.79) or BMI (p=0.98). We did not observe the previously reported interaction between age and genotype on fasting insulin (p=0.14). CONCLUSIONS/INTERPRETATION: Despite having greater statistical power, our data do not support the previously reported association between PARL Leu262Val and fasting plasma insulin levels, a measure of insulin resistance. Our findings indicate that this variant is unlikely to be an important contributor to insulin resistance in UK populations.

Adverse events experienced while transferring the critically ill patient from the emergency department to the intensive care unit.

OBJECTIVES: To determine the incidence and nature of adverse events and delay to patient transfer from emergency department to intensive care unit (ICU) in a metropolitan tertiary hospital. METHOD: A 6-month prospective observational study in conjunction with a retrospective chart audit on all emergency department patients admitted to ICU, including those admitted via theatre or after a computed tomography scan. RESULTS: Equipment problems was the most common adverse event occurring in 9% of patient transfers (n = 290). Hypothermia events occurred in 7% of transfers, cardiovascular events in 6% of patient transfers, delays to transfer >20 min occurred in 38% of the prospectively audited cases, with 14% waiting >1 h. One patient was found to have an incorrect patient identification band during a preoperative check. CONCLUSIONS: This study generally reported lower rates of adverse events than noted in previous studies involving critically ill transfers. The most significant finding was the application of an incorrect patient identification band and has prompted a review of practice. The establishment of benchmark indicators for adverse events and delays in transfer will be useful for future audits.

Inter-taxa differences in root uptake of 103/106Ru by plants.

Ruthenium-106 is of potential radioecological importance but soil-to-plant Transfer Factors for it are available only for few plant species. A Residual Maximum Likelihood (REML) procedure was used to construct a database of relative (103/106)Ru concentrations in 114 species of flowering plants including 106 species from experiments and 12 species from the literature (with 4 species in both). An Analysis of Variance (ANOVA), coded using a recent phylogeny for flowering plants, was used to identify a significant phylogenetic effect on relative mean (103/106)Ru concentrations in flowering plants. There were differences of 2,465-fold in the concentration to which plant species took up (103/106)Ru. Thirty-nine percent of the variance in inter-species differences could be ascribed to the taxonomic level of Order or above. Plants in the Orders Geraniales and Asterales had notably high uptake of (103/106)Ru compared to other plant groups. Plants on the Commelinoid monocot clades, and especially the Poaceae, had notably low uptake of (103/106)Ru. These data demonstrate that plant species are not independent units for (103/106)Ru concentrations but are linked through phylogeny. It is concluded that models of soil-to-plant transfer of (103/106)Ru should assume that; neither soil variables alone affect transfer nor plant species are independent units, and taking account of plant phylogeny might aid predictions of soil-to-plant transfer of (103/106)Ru, especially for species for which Transfer Factors are not available.

Death of a wild chimpanzee community member: possible outcome of intense sexual competition.

Our observations strongly support the view that a lethal gang attack occurred against a young adult male within a wild chimpanzee (Pan troglodytes schweinfurthii) community. This is the first report of such an occurrence. At least three adult male chimpanzees are believed to have been involved in the attack. This case is unusual because the attack was fatal, involved directed group aggression within a community, and was not linked to the overthrow of the alpha male. During the period before the attack, the percentage of cycling females in the community was very low (15 males:17 females, a maximum of two regularly cycling). This fact, coupled with the presence of one female in full genital tumescence, may have incited and escalated the violence of the attack. The lethal attack is interpreted as an act of intra-community male sexual competition resulting in the complete exclusion of one male from estrous females.

Postpartum affect and depressive symptoms in mothers and fathers.

In a study of the postpartum affective experiences of couples, mothers and fathers completed questionnaires on coping, marital satisfaction, stress, positive and negative affect, and depression one month pre- and then one month postpartum. More than one-fourth of both mothers and fathers reported elevated depressive symptoms, which correlated significantly between parents. Prepartum coping, stress, and affect significantly predicted postpartum affect. Research and clinical applications of the findings are discussed.

The relationship between childhood sexual abuse and subsequent onset of bulimia nervosa.

Seventy-two young adult women identified by the Bulimic Investigatory Test, Edinburgh (BITE) as having a high probability of suffering from bulimia nervosa were compared with 72 matched controls who did not display bulimic symptoms. Both groups were given measures of sexual abuse history, dissociation, and early family mealtime experiences. Rates of self-reported sexual abuse after the age of 12 with an adult relative as the perpetrator were significantly greater in the women diagnosed as bulimic. Nonsignificant but high rates of sexual abuse prior to age 12 were also found for this group. Dissociative experiences were also significantly more common in the bulimic group overall, and higher still for bulimic women who reported sexual abuse as children. Bulimic women had more negative and unusual mealtime experiences than nonbulimic women. Results suggest that sexual abuse may be related to the subsequent onset of bulimia nervosa for some women.

Correlates of bulimia nervosa: early family mealtime experiences.

The relationship of early mealtime experiences to later bulimia was investigated in a sample of 128 female college students. Significant group differences were found between bulimics, nonbulimics, and repeat dieters on an early meal experience questionnaire, with the bulimic group reporting the most negative and unusual experiences. Significant differences were also found between groups on depression but not on family adaptability or cohesion. These findings suggest that bulimic, repeat dieter, and nonbulimic females have differentiating histories regarding eating-related issues in the family context.

Parent preparation for the NICU-to-home transition: staff and parent perceptions.

Thirty-four nurses who were employed more than 20 hr per week in a neonatal intensive care unit (NICU) at a midwestern teaching hospital participated in a survey on discharge teaching. The survey consisted of 43 topics on which parents may or may not receive instruction prior to their baby's discharge from the NICU. For each topic, nurses were asked how important the topic is for parents to learn about prior to discharge, whether parents in the NICU are currently receiving instruction on the topic, and who among the NICU staff is responsible for instruction on the topic. Data obtained from this survey were compared to data obtained from a similar survey conducted 18 months previously with parents in the same NICU. Nurses rated the majority of topics in the survey as being very important for parents to learn about prior to their baby's discharge. For many of the items, a significant discrepancy existed between the percentage of nurses reporting the topics that were routinely taught and the percentage of parents who actually recalled learning about the topics. Suggestions on ways to improve the effectiveness of discharge teaching are provided.

Sexual attitudes and behavior of bulimic women: A preliminary investigation.

One-hundred and seventeen college-aged women at varying risk for developing bulimia answered a number of questions addressing their attitudes toward and comfort with sexuality as well as their sexual and contraceptive history. Risk for developing bulimia was unrelated to sexual attitudes and weakly related to greater comfort with sexuality. High-risk women reported first engaging in intercourse at earlier ages and were also more likely to engage in risky contraceptive behavior than their lower risk counterparts. Despite their tendency to report a greater number of previous sexual partners, high-risk women currently engaged and expected to continue to engage in sex less frequently than lower risk women. Taken together with previous findings, results suggest that the relationship between risk for bulimia and certain patterns of sexual behavior reflects a general cycle of impulsive and controlling behavior exhibited by high-risk women. Limitations and implications of the present study are discussed.

An observational study of affective and assertive family interactions during adolescence.

The purpose of this study was to describe the role of affective and assertive interactions in familial adaptation to adolescent pubertal development. Twenty-five family triads consisting of the adolescent and parents were asked to engage in an Unrevealed Differences task. This interaction was audiorecorded, and statements were coded as affective or assertive using a modified version of Bales's ([1970) Personality and Interpersonal Behavior, Holt, Rhinehart and Winston, Inc., New York) Interaction Process Analysis. Family relationships appear redefined during transpuberty, a time when physical changes associated with the onset of puberty are at apeak. This redefinition of transpubertal family relations is characterized by a decline in the use of affective statements coupled with an increase in the use of assertive statements relative to prepubertal and postpubertal families. Differences in mother-adolescent and father-adolescent interactions also are discussed.