Anaferon (pediatric formulation) in prophylactics of acute respiratory viral infection in children.

Prophylactic treatment with anaferon (pediatric formulation) in children groups reduced total morbidity and incidence of acute respiratory viral infections and shortened the duration of fever, intoxication, and catarrhal syndromes. No allergic and other reactions caused by administration of the preparation were noted.

Epitope Specificity of Monoclonal Antibodies to Porcine Immunoglobulin G and Light Chains.

Monoclonal antibodies to porcine IgG and light chains were produced and characterized. The specificity of selected mAbs was revealed by their selective reactivity with porcine immunoglobulins in ELISA, immunodiffusion, immunoelectrophoresis, and immunoblot analysis. For determining the presence of different IgG and light chains epitopes responsible for cross-reactivity (i.e. common epitopes) on the heterologous immunoglobulins, all mAbs were tested using sera and IgG from 12 animal species and man. All mAbs were used in unlabeled and HRP-labeled form in competitive ELISA to map the antigenic structure of the IgG and L chains. Four different epitopes shared by isotypes IgG1 and IgG2, and therefore specific for IgG class determinants, were recognized. In addition, four different epitopes specific for IgG1 were identified. Three mAbs are directed to different L-chains of porcine immunoglobulins, 2 mAbs recognized slower-migrating light chain L1, whereas one mAb reacted with the faster-migrating light chain L2 in porcine immunoglobulins. For the detection and quantitation of IgG1, a sandwich ELISA was developed. The availability of the set of mAbs to porcine immunoglobulin isotypes will stimulate and facilitate a further study of the porcine immune system.

Determination of Cross-Reactive (Common) Epitopes of Animal and Human Immunoglobulins Using Monoclonal Antibodies.

Monoclonal antibodies to cross-reactive epitopes of IgG and IgM of some animal species and man were produced. Specificity and cross-reactivity of the monoclonal antibodies were determined by ELISA, immunoblotting, immunodiffusion and immunoelectrophoresis. The presence of immunoglobulin epitopes common to some animal species and man that were localized on both native Ig molecules (conformational) and polypeptide chains (linear) was demonstrated. The use of cross-reactive monoclonal antibodies for quantitation and qualitation of animal and human Ig in biological fluids by different immunoassays has been showed.